Restriction map for
pFN21AE9547
(Circular) (Six-base) MAPSORT of: pFN21AE9547.seq Check: 9294 from: 1 to: 6364
pFN21AE9547 6364 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2703 4174 278
Size: 53 83 186 936 1167 1471 2468
Fragments arranged by size:
2468 1471 1167 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3707 1937
Size: 1770 4594
Acc65I G'GTAC_C
Cuts at: 3685 3685
Size: 6364
AclI AA'CG_TT
Cuts at: 1563 4491 4864 1563
Size: 2928 373 3063
Fragments arranged by size:
3063 2928 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3115 4427 5811 6079 1632
Size: 1483 1312 1384 268 1917
Fragments arranged by size:
1917 1483 1384 1312 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6167
AflIII A'CryG_T
Cuts at: 1203 2313 5263 6240 1203
Size: 1110 2950 977 1327
Fragments arranged by size:
2950 1327 1110 977
AgeI A'CCGG_T
Cuts at: 5195 5195
Size: 6364
AhdI GACnn_n'nnGTC
Cuts at: 5093 5093
Size: 6364
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6364
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6364
AlwNI CAG_nnn'CTG
Cuts at: 2033 2412 3396 4107 5679 2033
Size: 379 984 711 1572 2718
Fragments arranged by size:
2718 1572 984 711 379
ApaI G_GGCC'C
Cuts at: 2146 2146
Size: 6364
ApaLI G'TGCA_C
Cuts at: 2970 4421 5577 2970
Size: 1451 1156 3757
Fragments arranged by size:
3757 1451 1156
ApoI r'AATT_y
Cuts at: 1493 3084 3673 3899 4051 6160 1493
Size: 1591 589 226 152 2109 1697
Fragments arranged by size:
2109 1697 1591 589 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4918 160
Size: 872 3886 1606
Fragments arranged by size:
3886 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6364
AvaI C'yCGr_G
Cuts at: 1870 1943 3579 3689 1870
Size: 73 1636 110 4545
Fragments arranged by size:
4545 1636 110 73
AvrII C'CTAG_G
Cuts at: 3424 3424
Size: 6364
BamHI G'GATC_C
Cuts at: 2201 3694 4061 2201
Size: 1493 367 4504
Fragments arranged by size:
4504 1493 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2819 3545 3685 5140 618
Size: 325 217 627 1032 726 140 1455 1842
Fragments arranged by size:
1842 1455 1032 726 627 325 217 140
BanII G_rGCy'C
Cuts at: 1391 2146 2236 2393 2877 3281 3683 1391
Size: 755 90 157 484 404 402 4072
Fragments arranged by size:
4072 755 484 404 402 157 90
BbeI G_GCGC'C
Cuts at: 3549 3549
Size: 6364
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2698 928
Size: 971 799 4594
Fragments arranged by size:
4594 971 799
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4555 4589 1903
Size: 34 2618 34 3678
Fragments arranged by size:
3678 2618 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2552 4256 5466 2552
Size: 1704 1210 3450
Fragments arranged by size:
3450 1704 1210
BclI T'GATC_A
Cuts at: 1548 2305 3223 1548
Size: 757 918 4689
Fragments arranged by size:
4689 918 757
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3384 4975 136
Size: 107 122 71 631 605 1712 1591 1525
Fragments arranged by size:
1712 1591 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6359 6359
Size: 6364
BlpI GC'TnA_GC
Cuts at: 3339 3781 3339
Size: 442 5922
Bme1580I G_kGCm'C
Cuts at: 1790 2146 2974 4425 5581 1790
Size: 356 828 1451 1156 2573
Fragments arranged by size:
2573 1451 1156 828 356
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6364
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3364 4095 5053
Size: 416 53 83 399 24 1947 731 958
Cuts at: 5053 442
Size: 1753
Fragments arranged by size:
1947 1753 958 731 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3374 1056
Size: 2318 4046
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2282 2282
Size: 6364
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2891 2999 5024 1239
Size: 1652 108 2025 2579
Fragments arranged by size:
2579 2025 1652 108
Bpu10I CC'TnA_GC
Cuts at: 2392 2421 2392
Size: 29 6335
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2231 2252 3074 4489 5354 5652 2231
Size: 21 822 1415 865 298 2943
Fragments arranged by size:
2943 1415 865 822 298 21
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5027 882
Size: 617 3528 2219
Fragments arranged by size:
3528 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2314 4155 493
Size: 711 1110 1841 2702
Fragments arranged by size:
2702 1841 1110 711
BsaBI GATnn'nnATC
Cuts at: 6358 6358
Size: 6364
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2064 2700 3546 4171
Size: 53 83 186 936 531 636 846 625
Cuts at: 4171 4553 5188 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 846 636 635 625 531 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 3417 4797 5195 5469 5616 6027 3417
Size: 1380 398 274 147 411 3754
Fragments arranged by size:
3754 1380 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2705 2735 1178
Size: 30 165 30 1302 30 4807
Fragments arranged by size:
4807 1302 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2591 3653 1186
Size: 1405 1062 3897
Fragments arranged by size:
3897 1405 1062
BseYI C'CCAG_C
Cuts at: 2011 3541 3549 5567 6063 2011
Size: 1530 8 2018 496 2312
Fragments arranged by size:
2312 2018 1530 496 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2611 3622 1163
Size: 573 875 1011 3905
Fragments arranged by size:
3905 1011 875 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3814 4575 5603 1540
Size: 275 186 1813 761 1028 2301
Fragments arranged by size:
2301 1813 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2135 2393 2974 3281 4425 4510 5581 2135
Size: 258 581 307 1144 85 1071 2918
Fragments arranged by size:
2918 1144 1071 581 307 258 85
BsmI GAATG_Cn'
Cuts at: 2483 3875 3968 2483
Size: 1392 93 4879
Fragments arranged by size:
4879 1392 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2135 2146 2236 2393 2877 2974 3281
Size: 399 345 11 90 157 484 97 307
Cuts at: 3281 3683 4425 4510 5581 1391
Size: 402 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 484 402 399 345 307
157 97 90 85 11
BspEI T'CCGG_A
Cuts at: 6027 6027
Size: 6364
BspHI T'CATG_A
Cuts at: 3193 4253 5983 3193
Size: 1060 1730 3574
Fragments arranged by size:
3574 1730 1060
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2922 3719 844
Size: 2078 797 3489
Fragments arranged by size:
3489 2078 797
BsrBI CCG'CTC
Cuts at: 1394 1823 2787 4251 1394
Size: 429 964 1464 3507
Fragments arranged by size:
3507 1464 964 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4859 5033 65
Size: 1183 3611 174 1396
Fragments arranged by size:
3611 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5008 5195 1700
Size: 110 3198 187 2869
Fragments arranged by size:
3198 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2547 96
Size: 2451 3913
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6364
BssSI C'ACGA_G
Cuts at: 2069 4424 5436 2069
Size: 2355 1012 2997
Fragments arranged by size:
2997 2355 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6364
BstBI TT'CG_AA
Cuts at: 2614 5168 2614
Size: 2554 3810
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6364
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 3634 2011
Size: 1623 4741
BstYI r'GATC_y
Cuts at: 1972 2201 3198 3694 4061 4446 4463 5915 6001
Size: 229 997 496 367 385 17 1452 86
Cuts at: 6001 6013 6359 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 997 496 385 367 346 229
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2079 2451 2553 3621 3633 513
Size: 552 1014 372 102 1068 12 3244
Fragments arranged by size:
3244 1068 1014 552 372 102 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5625
Fragments arranged by size:
5625 683 56
BtsI GCAGTG_nn'
Cuts at: 2410 2623 3610 3880 4674 4694 2410
Size: 213 987 270 794 20 4080
Fragments arranged by size:
4080 987 794 270 213 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6364
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6329
DraI TTT'AAA
Cuts at: 3668 3668
Size: 6364
DraIII CAC_nnn'GTG
Cuts at: 2991 2991
Size: 6364
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5371 809
Size: 507 4055 1802
Fragments arranged by size:
4055 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3624 3811 4700
Size: 54 1425 50 275 18 1794 187 889
Cuts at: 4700 6329 8
Size: 1629 43
Fragments arranged by size:
1794 1629 1425 889 275 187 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3811 1537
Size: 275 1999 4090
Fragments arranged by size:
4090 1999 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2379 2588 3209 3645 4294 1353
Size: 1026 209 621 436 649 3423
Fragments arranged by size:
3423 1026 649 621 436 209
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4951 5325 5471 387
Size: 1064 138 3362 374 146 1280
Fragments arranged by size:
3362 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2391 3279 2391
Size: 888 5476
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2891 2999 3115 4427 5024 5811 6079
Size: 393 1259 108 116 1312 597 787 268
Cuts at: 6079 1239
Size: 1524
Fragments arranged by size:
1524 1312 1259 787 597 393 268 116
108
EcoNI CCTnn'n_nnAGG
Cuts at: 2037 2122 2210 2037
Size: 85 88 6191
Fragments arranged by size:
6191 88 85
EcoO109I rG'GnC_Cy
Cuts at: 2030 2143 2154 2400 3038 2030
Size: 113 11 246 638 5356
Fragments arranged by size:
5356 638 246 113 11
EcoRI G'AATT_C
Cuts at: 3673 4051 3673
Size: 378 5986
FspI TGC'GCA
Cuts at: 4870 4870
Size: 6364
HaeII r_GCGC'y
Cuts at: 1678 3465 3549 5511 1678
Size: 1787 84 1962 2531
Fragments arranged by size:
2531 1962 1787 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2095 2127 2290 2322 3384
Size: 32 135 32 523 32 163 32 1062
Cuts at: 3384 3416 5005 5037 5079 5111 1373
Size: 32 1589 32 42 32 2626
Fragments arranged by size:
2626 1589 1062 523 163 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2532 3270 3708 3954 669
Size: 988 281 594 738 438 246 3079
Fragments arranged by size:
3079 988 738 594 438 281 246
HindIII A'AGCT_T
Cuts at: 748 3092 3470 748
Size: 2344 378 3642
Fragments arranged by size:
3642 2344 378
HpaI GTT'AAC
Cuts at: 3954 3954
Size: 6364
KasI G'GCGC_C
Cuts at: 3545 3545
Size: 6364
KpnI G_GTAC'C
Cuts at: 3689 3689
Size: 6364
MfeI C'AATT_G
Cuts at: 3963 3963
Size: 6364
MluI A'CGCG_T
Cuts at: 6240 6240
Size: 6364
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5478 5662 1305
Size: 4173 184 2007
Fragments arranged by size:
4173 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3626 10
Size: 54 1425 343 1794 2748
Fragments arranged by size:
2748 1794 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2645 4322 4681 4840 518
Size: 648 1479 1677 359 159 2042
Fragments arranged by size:
2042 1677 1479 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2542 3780 4457 5605 5850 1606
Size: 936 1238 677 1148 245 2120
Fragments arranged by size:
2120 1238 1148 936 677 245
NarI GG'CG_CC
Cuts at: 3546 3546
Size: 6364
NcoI C'CATG_G
Cuts at: 513 1065 2079 3621 3633 513
Size: 552 1014 1542 12 3244
Fragments arranged by size:
3244 1542 1014 552 12
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6364
NheI G'CTAG_C
Cuts at: 1052 3370 1052
Size: 2318 4046
NotI GC'GGCC_GC
Cuts at: 3811 3811
Size: 6364
NspI r_CATG'y
Cuts at: 1132 1229 2253 3722 5267 1132
Size: 97 1024 1469 1545 2229
Fragments arranged by size:
2229 1545 1469 1024 97
PasI CC'CwG_GG
Cuts at: 1348 2097 2147 2955 1348
Size: 749 50 808 4757
Fragments arranged by size:
4757 808 749 50
PciI A'CATG_T
Cuts at: 5263 5263
Size: 6364
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6364
PfoI T'CCnGG_A
Cuts at: 2335 2335
Size: 6364
PmeI GTTT'AAAC
Cuts at: 3668 3668
Size: 6364
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4433 4465 5946 5978 4433
Size: 32 1481 32 4819
Fragments arranged by size:
4819 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3038 3038
Size: 6364
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6364
PsiI TTA'TAA
Cuts at: 3141 3934 3141
Size: 793 5571
PspOMI G'GGCC_C
Cuts at: 2142 2142
Size: 6364
PstI C_TGCA'G
Cuts at: 830 3392 3486 3716 830
Size: 2562 94 230 3478
Fragments arranged by size:
3478 2562 230 94
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6364
PvuII CAG'CTG
Cuts at: 2542 2542
Size: 6364
SacI G_AGCT'C
Cuts at: 2393 3281 2393
Size: 888 5476
SalI G'TCGA_C
Cuts at: 1936 3706 1936
Size: 1770 4594
SbfI CC_TGCA'GG
Cuts at: 3716 3716
Size: 6364
ScaI AGT'ACT
Cuts at: 4612 6324 4612
Size: 1712 4652
SfcI C'TryA_G
Cuts at: 826 1045 2404 3388 3482 3712 4847 5528 5719
Size: 219 1359 984 94 230 1135 681 191
Cuts at: 5719 826
Size: 1471
Fragments arranged by size:
1471 1359 1135 984 681 230 219 191
94
SfoI GGC'GCC
Cuts at: 3547 3547
Size: 6364
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6364
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6364
SmaI CCC'GGG
Cuts at: 1872 3581 3691 1872
Size: 1709 110 4545
Fragments arranged by size:
4545 1709 110
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2210 2267 3089 4468 5369 5631
Size: 197 926 267 57 822 1379 901 262
Cuts at: 5631 820
Size: 1553
Fragments arranged by size:
1553 1379 926 901 822 267 262 197
57
SnaBI TAC'GTA
Cuts at: 493 4155 493
Size: 3662 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6364
SphI G_CATG'C
Cuts at: 1132 3722 1132
Size: 2590 3774
SspI AAT'ATT
Cuts at: 5 52 4288 5
Size: 47 4236 2081
Fragments arranged by size:
4236 2081 47
StuI AGG'CCT
Cuts at: 1517 2731 2850 1517
Size: 1214 119 5031
Fragments arranged by size:
5031 1214 119
StyI C'CwwG_G
Cuts at: 513 1065 2079 2372 3424 3621 3633 3803 513
Size: 552 1014 293 1052 197 12 170 3074
Fragments arranged by size:
3074 1052 1014 552 293 197 170 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2736 2807 4391 4408 4561 4746 5191
Size: 1255 1156 71 1584 17 153 185 445
Cuts at: 5191 325
Size: 1498
Fragments arranged by size:
1584 1498 1255 1156 445 185 153 71
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2238 2547 2686
Size: 274 80 33 51 1443 261 309 139
Cuts at: 2686 4610 6322 96
Size: 1924 1712 138
Fragments arranged by size:
1924 1712 1443 309 274 261 139 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2869 2901 6324 6356 2869
Size: 32 3423 32 2877
Fragments arranged by size:
3423 2877 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2702 1316
Size: 1386 4978
XbaI T'CTAG_A
Cuts at: 3700 3700
Size: 6364
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6364
XmaI C'CCGG_G
Cuts at: 1870 3579 3689 1870
Size: 1709 110 4545
Fragments arranged by size:
4545 1709 110
XmnI GAAnn'nnTTC
Cuts at: 3413 4493 3413
Size: 1080 5284
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2701 4172 276
Size: 53 83 186 936 1167 1471 2468
Fragments arranged by size:
2468 1471 1167 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbvCI BfrBI BsiWI
BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI