Restriction map for
pFN21AE9546
(Circular) (Six-base) MAPSORT of: pFN21AE9546.seq Check: 5375 from: 1 to: 6271
pFN21AE9546 6271 bp
With 182 enzymes: SgfI *
December 28, 2011 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2061 2182 4081 278
Size: 53 83 186 936 525 121 1899 2468
Fragments arranged by size:
2468 1899 936 525 186 121 83 53
AccI GT'mk_AC
Cuts at: 1937 2754 2919 3614 1937
Size: 817 165 695 4594
Fragments arranged by size:
4594 817 695 165
Acc65I G'GTAC_C
Cuts at: 3592 3592
Size: 6271
AclI AA'CG_TT
Cuts at: 1563 4398 4771 1563
Size: 2835 373 3063
Fragments arranged by size:
3063 2835 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2322 2688 3034 4334 5718 5986 1632
Size: 690 366 346 1300 1384 268 1917
Fragments arranged by size:
1917 1384 1300 690 366 346 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6074
AflIII A'CryG_T
Cuts at: 1203 2857 5170 6147 1203
Size: 1654 2313 977 1327
Fragments arranged by size:
2313 1654 1327 977
AgeI A'CCGG_T
Cuts at: 5102 5102
Size: 6271
AhdI GACnn_n'nnGTC
Cuts at: 5000 5000
Size: 6271
AleI CACnn'nnGTG
Cuts at: 1166 2972 1166
Size: 1806 4465
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6271
AlwNI CAG_nnn'CTG
Cuts at: 2241 4014 5586 2241
Size: 1773 1572 2926
Fragments arranged by size:
2926 1773 1572
ApaLI G'TGCA_C
Cuts at: 4328 5484 4328
Size: 1156 5115
ApoI r'AATT_y
Cuts at: 1493 3039 3325 3580 3806 3958 6067 1493
Size: 1546 286 255 226 152 2109 1697
Fragments arranged by size:
2109 1697 1546 286 255 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2400 4825 160
Size: 872 1368 2425 1606
Fragments arranged by size:
2425 1606 1368 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6271
AvaI C'yCGr_G
Cuts at: 1870 1943 3596 1870
Size: 73 1653 4545
Fragments arranged by size:
4545 1653 73
BamHI G'GATC_C
Cuts at: 3601 3968 3601
Size: 367 5904
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2040 2925 3592 5047 618
Size: 325 217 627 253 885 667 1455 1842
Fragments arranged by size:
1842 1455 885 667 627 325 253 217
BanII G_rGCy'C
Cuts at: 1391 2293 3590 1391
Size: 902 1297 4072
Fragments arranged by size:
4072 1297 902
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2940 928
Size: 971 1041 4259
Fragments arranged by size:
4259 1041 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4462 4496 1903
Size: 34 2525 34 3678
Fragments arranged by size:
3678 2525 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4163 5373 4163
Size: 1210 5061
BclI T'GATC_A
Cuts at: 1548 2805 1548
Size: 1257 5014
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2288 4882 136
Size: 107 122 71 631 605 616 2594 1525
Fragments arranged by size:
2594 1525 631 616 605 122 107 71
BglII A'GATC_T
Cuts at: 2961 6266 2961
Size: 3305 2966
BlpI GC'TnA_GC
Cuts at: 3688 3688
Size: 6271
Bme1580I G_kGCm'C
Cuts at: 1790 4332 5488 1790
Size: 2542 1156 2573
Fragments arranged by size:
2573 2542 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6271
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4002 4960 442
Size: 416 53 83 399 24 2585 958 1753
Fragments arranged by size:
2585 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6271
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2906 2906
Size: 6271
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2155 2168 2239 3344 4931 1239
Size: 916 13 71 1105 1587 2579
Fragments arranged by size:
2579 1587 1105 916 71 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2421 4396 5261 5559 2421
Size: 1975 865 298 3133
Fragments arranged by size:
3133 1975 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4934 882
Size: 617 3435 2219
Fragments arranged by size:
3435 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3100 4062 493
Size: 711 1896 962 2702
Fragments arranged by size:
2702 1896 962 711
BsaBI GATnn'nnATC
Cuts at: 6265 6265
Size: 6271
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2058 2179 4078 4460
Size: 53 83 186 936 525 121 1899 382
Cuts at: 4460 5095 275
Size: 635 1451
Fragments arranged by size:
1899 1451 936 635 525 382 186 121
83 53
BsaWI w'CCGG_w
Cuts at: 2071 4704 5102 5376 5523 5934 2071
Size: 2633 398 274 147 411 2408
Fragments arranged by size:
2633 2408 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6046
Fragments arranged by size:
6046 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6271
BseYI C'CCAG_C
Cuts at: 2026 2185 5474 5970 2026
Size: 159 3289 496 2327
Fragments arranged by size:
3289 2327 496 159
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2052 1163
Size: 573 316 5382
Fragments arranged by size:
5382 573 316
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3721 4482 5510 1540
Size: 275 186 1720 761 1028 2301
Fragments arranged by size:
2301 1720 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2104 3426 4332 4417 5488 2104
Size: 1322 906 85 1071 2887
Fragments arranged by size:
2887 1322 1071 906 85
BsmI GAATG_Cn'
Cuts at: 3275 3782 3875 3275
Size: 507 93 5671
Fragments arranged by size:
5671 507 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2065 2065
Size: 6271
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2104 2293 3426 3590 4332 4417 5488
Size: 399 314 189 1133 164 742 85 1071
Cuts at: 5488 1391
Size: 2174
Fragments arranged by size:
2174 1133 1071 742 399 314 189 164
85
BspEI T'CCGG_A
Cuts at: 5934 5934
Size: 6271
BspHI T'CATG_A
Cuts at: 2310 4160 5890 2310
Size: 1850 1730 2691
Fragments arranged by size:
2691 1850 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3626 844
Size: 2782 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2139 4158 1394
Size: 429 316 2019 3507
Fragments arranged by size:
3507 2019 429 316
BsrDI GCAATG_nn'
Cuts at: 65 1248 4766 4940 65
Size: 1183 3518 174 1396
Fragments arranged by size:
3518 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2196 4915 5102 1700
Size: 110 386 2719 187 2869
Fragments arranged by size:
2869 2719 386 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6271
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6271
BssSI C'ACGA_G
Cuts at: 4331 5343 4331
Size: 1012 5259
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2601 794
Size: 1807 4464
BstBI TT'CG_AA
Cuts at: 5075 5075
Size: 6271
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6271
BstXI CCAn_nnnn'nTGG
Cuts at: 2922 2922
Size: 6271
BstYI r'GATC_y
Cuts at: 1972 2961 3601 3968 4353 4370 5822 5908 5920
Size: 989 640 367 385 17 1452 86 12
Cuts at: 5920 6266 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 989 640 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2166 3334 513
Size: 552 1101 1168 3450
Fragments arranged by size:
3450 1168 1101 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5532
Fragments arranged by size:
5532 683 56
BtsI GCAGTG_nn'
Cuts at: 2615 3098 3295 3568 3787 4581 4601 2615
Size: 483 197 273 219 794 20 4285
Fragments arranged by size:
4285 794 483 273 219 197 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6271
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2653 2688 529
Size: 35 2089 35 4112
Fragments arranged by size:
4112 2089 35 35
DraI TTT'AAA
Cuts at: 2764 3575 2764
Size: 811 5460
DraIII CAC_nnn'GTG
Cuts at: 2661 3103 2661
Size: 442 5829
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5278 809
Size: 507 3962 1802
Fragments arranged by size:
3962 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2031 2571 2642
Size: 54 1425 50 275 18 201 540 71
Cuts at: 2642 2939 3718 4607 6236 8
Size: 297 779 889 1629 43
Fragments arranged by size:
1629 1425 889 779 540 297 275 201
71 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3718 1537
Size: 275 1906 4090
Fragments arranged by size:
4090 1906 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2341 2561 3053 4201 1353
Size: 988 220 492 1148 3423
Fragments arranged by size:
3423 1148 988 492 220
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3320 4858 5232 5378 387
Size: 1064 138 1731 1538 374 146 1280
Fragments arranged by size:
1731 1538 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2155 2168 2239 2322 2688 3034 3344
Size: 393 523 13 71 83 366 346 310
Cuts at: 3344 4334 4931 5718 5986 1239
Size: 990 597 787 268 1524
Fragments arranged by size:
1524 990 787 597 523 393 366 346
310 268 83 71 13
EcoNI CCTnn'n_nnAGG
Cuts at: 3069 3069
Size: 6271
EcoO109I rG'GnC_Cy
Cuts at: 2815 2815
Size: 6271
EcoRI G'AATT_C
Cuts at: 3580 3958 3580
Size: 378 5893
FspI TGC'GCA
Cuts at: 4777 4777
Size: 6271
HaeII r_GCGC'y
Cuts at: 1678 5418 1678
Size: 3740 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2048 2080 2318 2350 4912
Size: 32 135 32 476 32 238 32 2562
Cuts at: 4912 4944 4986 5018 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2562 476 238 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3615 3861 669
Size: 988 281 1677 246 3079
Fragments arranged by size:
3079 1677 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3348 3393 748
Size: 2600 45 3626
Fragments arranged by size:
3626 2600 45
HpaI GTT'AAC
Cuts at: 3861 3861
Size: 6271
KpnI G_GTAC'C
Cuts at: 3596 3596
Size: 6271
MfeI C'AATT_G
Cuts at: 2724 3870 2724
Size: 1146 5125
MluI A'CGCG_T
Cuts at: 6147 6147
Size: 6271
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5385 5569 1305
Size: 4080 184 2007
Fragments arranged by size:
4080 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2573 2644 2941 10
Size: 54 1425 343 741 71 297 3340
Fragments arranged by size:
3340 1425 741 343 297 71 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2801 2831 2972 3131 4229 4588 4747
Size: 648 1635 30 141 159 1098 359 159
Cuts at: 4747 518
Size: 2042
Fragments arranged by size:
2042 1635 1098 648 359 159 159 141
30
MspA1I CmG'CkG
Cuts at: 1606 2030 2037 2168 3687 4364 5512 5757 1606
Size: 424 7 131 1519 677 1148 245 2120
Fragments arranged by size:
2120 1519 1148 677 424 245 131 7
NaeI GCC'GGC
Cuts at: 2198 2198
Size: 6271
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5719
NdeI CA'TA_TG
Cuts at: 387 2535 387
Size: 2148 4123
NgoMIV G'CCGG_C
Cuts at: 2196 2196
Size: 6271
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6271
NotI GC'GGCC_GC
Cuts at: 3718 3718
Size: 6271
NspI r_CATG'y
Cuts at: 1132 1229 2608 2861 3629 5174 1132
Size: 97 1379 253 768 1545 2229
Fragments arranged by size:
2229 1545 1379 768 253 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6271
PciI A'CATG_T
Cuts at: 2857 5170 2857
Size: 2313 3958
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2749 1108
Size: 1641 4630
PfoI T'CCnGG_A
Cuts at: 2046 3107 3450 2046
Size: 1061 343 4867
Fragments arranged by size:
4867 1061 343
PmeI GTTT'AAAC
Cuts at: 3575 3575
Size: 6271
PmlI CAC'GTG
Cuts at: 3100 3100
Size: 6271
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4340 4372 5853 5885 4340
Size: 32 1481 32 4726
Fragments arranged by size:
4726 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2815 2815
Size: 6271
PshAI GACnn'nnGTC
Cuts at: 1613 2178 1613
Size: 565 5706
PsiI TTA'TAA
Cuts at: 3841 3841
Size: 6271
PstI C_TGCA'G
Cuts at: 830 2252 2848 3061 3234 3623 830
Size: 1422 596 213 173 389 3478
Fragments arranged by size:
3478 1422 596 389 213 173
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6271
PvuII CAG'CTG
Cuts at: 2037 2037
Size: 6271
SacII CC_GC'GG
Cuts at: 2169 2169
Size: 6271
SalI G'TCGA_C
Cuts at: 1936 3613 1936
Size: 1677 4594
SbfI CC_TGCA'GG
Cuts at: 3623 3623
Size: 6271
ScaI AGT'ACT
Cuts at: 4519 6231 4519
Size: 1712 4559
SfcI C'TryA_G
Cuts at: 826 1045 2248 2730 2844 3057 3230 3619 4754
Size: 219 1203 482 114 213 173 389 1135
Cuts at: 4754 5435 5626 826
Size: 681 191 1471
Fragments arranged by size:
1471 1203 1135 681 482 389 219 213
191 173 114
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6271
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6271
SmaI CCC'GGG
Cuts at: 1872 3598 1872
Size: 1726 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2436 4375 5276 5538 820
Size: 197 926 493 1939 901 262 1553
Fragments arranged by size:
1939 1553 926 901 493 262 197
SnaBI TAC'GTA
Cuts at: 493 4062 493
Size: 3569 2702
SpeI A'CTAG_T
Cuts at: 152 2587 152
Size: 2435 3836
SphI G_CATG'C
Cuts at: 1132 2608 3629 1132
Size: 1476 1021 3774
Fragments arranged by size:
3774 1476 1021
SspI AAT'ATT
Cuts at: 5 52 2404 2629 2876 4195 5
Size: 47 2352 225 247 1319 2081
Fragments arranged by size:
2352 2081 1319 247 225 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6271
StyI C'CwwG_G
Cuts at: 513 1065 3710 513
Size: 552 2645 3074
Fragments arranged by size:
3074 2645 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4298 4315 4468 4653 5098 325
Size: 1255 2718 17 153 185 445 1498
Fragments arranged by size:
2718 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2388 4517 6229
Size: 274 80 33 51 1443 411 2129 1712
Cuts at: 6229 96
Size: 138
Fragments arranged by size:
2129 1712 1443 411 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2094 2126 2487 2519 3089 3121 6231 6263 2094
Size: 32 361 32 570 32 3110 32 2102
Fragments arranged by size:
3110 2102 570 361 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6271
XbaI T'CTAG_A
Cuts at: 3607 3607
Size: 6271
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2034 2992 2034
Size: 958 5313
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6271
XmaI C'CCGG_G
Cuts at: 1870 3596 1870
Size: 1726 4545
XmnI GAAnn'nnTTC
Cuts at: 2012 4400 2012
Size: 2388 3883
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2059 2180 4079 276
Size: 53 83 186 936 525 121 1899 2468
Fragments arranged by size:
2468 1899 936 525 186 121 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI
SfiI SfoI SrfI SwaI