Restriction map for
pFN21AE9543
(Circular) (Six-base) MAPSORT of: pFN21AE9543.seq Check: 2480 from: 1 to: 6193
pFN21AE9543 6193 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4003 278
Size: 53 83 186 936 2467 2468
Fragments arranged by size:
2468 2467 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3536 1937
Size: 1599 4594
Acc65I G'GTAC_C
Cuts at: 3514 3514
Size: 6193
AclI AA'CG_TT
Cuts at: 1563 4320 4693 1563
Size: 2757 373 3063
Fragments arranged by size:
3063 2757 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2993 3034 4256 5640 5908 1632
Size: 1361 41 1222 1384 268 1917
Fragments arranged by size:
1917 1384 1361 1222 268 41
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5996
AflIII A'CryG_T
Cuts at: 1203 2189 5092 6069 1203
Size: 986 2903 977 1327
Fragments arranged by size:
2903 1327 986 977
AgeI A'CCGG_T
Cuts at: 5024 5024
Size: 6193
AhdI GACnn_n'nnGTC
Cuts at: 4922 4922
Size: 6193
AleI CACnn'nnGTG
Cuts at: 1166 2709 1166
Size: 1543 4650
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6193
AlwNI CAG_nnn'CTG
Cuts at: 3936 5508 3936
Size: 1572 4621
ApaLI G'TGCA_C
Cuts at: 2018 4250 5406 2018
Size: 2232 1156 2805
Fragments arranged by size:
2805 2232 1156
ApoI r'AATT_y
Cuts at: 1493 3502 3728 3880 5989 1493
Size: 2009 226 152 2109 1697
Fragments arranged by size:
2109 2009 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4747 160
Size: 872 3715 1606
Fragments arranged by size:
3715 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6193
AvaI C'yCGr_G
Cuts at: 1870 1943 3518 1870
Size: 73 1575 4545
Fragments arranged by size:
4545 1575 73
BamHI G'GATC_C
Cuts at: 3523 3890 3523
Size: 367 5826
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3416 3514 4969 618
Size: 325 217 627 1629 98 1455 1842
Fragments arranged by size:
1842 1629 1455 627 325 217 98
BanII G_rGCy'C
Cuts at: 1391 2244 3091 3257 3512 1391
Size: 853 847 166 255 4072
Fragments arranged by size:
4072 853 847 255 166
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2605 928
Size: 971 706 4516
Fragments arranged by size:
4516 971 706
BbvCI CC'TCA_GC
Cuts at: 2597 2597
Size: 6193
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2479 2513 4384 4418 1903
Size: 34 542 34 1871 34 3678
Fragments arranged by size:
3678 1871 542 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4085 5295 4085
Size: 1210 4983
BclI T'GATC_A
Cuts at: 1548 2928 1548
Size: 1380 4813
BfrBI ATG'CAT
Cuts at: 2068 2068
Size: 6193
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4804 136
Size: 107 122 71 631 605 3132 1525
Fragments arranged by size:
3132 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2323 6188 2323
Size: 3865 2328
BlpI GC'TnA_GC
Cuts at: 3090 3610 3090
Size: 520 5673
Bme1580I G_kGCm'C
Cuts at: 1790 2022 3419 4254 5410 1790
Size: 232 1397 835 1156 2573
Fragments arranged by size:
2573 1397 1156 835 232
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6193
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2442 3924 4882
Size: 416 53 83 399 24 1025 1482 958
Cuts at: 4882 442
Size: 1753
Fragments arranged by size:
1753 1482 1025 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6193
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2129 2142 3000 4853 1239
Size: 890 13 858 1853 2579
Fragments arranged by size:
2579 1853 890 858 13
Bpu10I CC'TnA_GC
Cuts at: 2238 2597 3434 2238
Size: 359 837 4997
Fragments arranged by size:
4997 837 359
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4318 5183 5481 4318
Size: 865 298 5030
Fragments arranged by size:
5030 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2270 2768 4856 882
Size: 617 771 498 2088 2219
Fragments arranged by size:
2219 2088 771 617 498
BsaAI yAC'GTr
Cuts at: 493 1204 2190 3984 493
Size: 711 986 1794 2702
Fragments arranged by size:
2702 1794 986 711
BsaBI GATnn'nnATC
Cuts at: 6187 6187
Size: 6193
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4000 4382 5017 275
Size: 53 83 186 936 2467 382 635 1451
Fragments arranged by size:
2467 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2538 4626 5024 5298 5445 5856 2538
Size: 2088 398 274 147 411 2875
Fragments arranged by size:
2875 2088 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2300 2330 1178
Size: 30 165 30 897 30 5041
Fragments arranged by size:
5041 897 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2030 1186
Size: 844 5349
BseYI C'CCAG_C
Cuts at: 2823 2892 3244 3250 3420 5396 5892 2823
Size: 69 352 6 170 1976 496 3124
Fragments arranged by size:
3124 1976 496 352 170 69 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2341 2531 2536 3020 3080 1163
Size: 573 605 190 5 484 60 4276
Fragments arranged by size:
4276 605 573 484 190 60 5
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3643 4404 5432 1540
Size: 275 186 1642 761 1028 2301
Fragments arranged by size:
2301 1642 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2022 2244 4254 4339 5410 2022
Size: 222 2010 85 1071 2805
Fragments arranged by size:
2805 2010 1071 222 85
BsmI GAATG_Cn'
Cuts at: 2068 3704 3797 2068
Size: 1636 93 4464
Fragments arranged by size:
4464 1636 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3393 3393
Size: 6193
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2244 3091 3257 3419 3512 4254
Size: 399 232 222 847 166 162 93 742
Cuts at: 4254 4339 5410 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 847 742 399 232 222 166
162 93 85
BspEI T'CCGG_A
Cuts at: 2538 5856 2538
Size: 3318 2875
BspHI T'CATG_A
Cuts at: 4082 5812 4082
Size: 1730 4463
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3548 844
Size: 2704 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4080 1394
Size: 429 2257 3507
Fragments arranged by size:
3507 2257 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3037 4688 4862 65
Size: 1183 1789 1651 174 1396
Fragments arranged by size:
1789 1651 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4837 5024 1700
Size: 110 3027 187 2869
Fragments arranged by size:
3027 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6193
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6193
BssSI C'ACGA_G
Cuts at: 3233 4253 5265 3233
Size: 1020 1012 4161
Fragments arranged by size:
4161 1020 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2658 794
Size: 1864 4329
BstBI TT'CG_AA
Cuts at: 4997 4997
Size: 6193
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6193
BstXI CCAn_nnnn'nTGG
Cuts at: 2107 2518 2107
Size: 411 5782
BstYI r'GATC_y
Cuts at: 1972 2115 2323 3523 3890 4275 4292 5744 5830
Size: 143 208 1200 367 385 17 1452 86
Cuts at: 5830 5842 6188 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1200 385 367 346 208 143
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2517 513
Size: 552 939 513 4189
Fragments arranged by size:
4189 939 552 513
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5454
Fragments arranged by size:
5454 683 56
BtsI GCAGTG_nn'
Cuts at: 2511 2548 2656 3406 3709 4503 4523 2511
Size: 37 108 750 303 794 20 4181
Fragments arranged by size:
4181 794 750 303 108 37 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6193
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6158
DraI TTT'AAA
Cuts at: 3497 3497
Size: 6193
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5200 809
Size: 507 3884 1802
Fragments arranged by size:
3884 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3393 3486 3640
Size: 54 1425 50 275 18 1563 93 154
Cuts at: 3640 4529 6158 8
Size: 889 1629 43
Fragments arranged by size:
1629 1563 1425 889 275 154 93 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3640 1537
Size: 275 1828 4090
Fragments arranged by size:
4090 1828 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2249 2762 4123 1353
Size: 896 513 1361 3423
Fragments arranged by size:
3423 1361 896 513
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4780 5154 5300 387
Size: 1064 138 3191 374 146 1280
Fragments arranged by size:
3191 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2242 2242
Size: 6193
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2129 2142 2993 3000 3034 4256 4853
Size: 393 497 13 851 7 34 1222 597
Cuts at: 4853 5640 5908 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1222 851 787 597 497 393 268
34 13 7
EcoO109I rG'GnC_Cy
Cuts at: 2467 2746 2829 2936 2467
Size: 279 83 107 5724
Fragments arranged by size:
5724 279 107 83
EcoRI G'AATT_C
Cuts at: 3502 3880 3502
Size: 378 5815
FspI TGC'GCA
Cuts at: 4699 4699
Size: 6193
HaeII r_GCGC'y
Cuts at: 1678 5340 1678
Size: 3662 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2300 2332 2357 2389 4834
Size: 32 135 32 728 32 25 32 2445
Cuts at: 4834 4866 4908 4940 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2445 728 135 42 32 32 32
32 32 32 25
HincII GTy'rAC
Cuts at: 669 1657 1938 2962 3537 3783 669
Size: 988 281 1024 575 246 3079
Fragments arranged by size:
3079 1024 988 575 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6193
HpaI GTT'AAC
Cuts at: 2962 3783 2962
Size: 821 5372
KpnI G_GTAC'C
Cuts at: 3518 3518
Size: 6193
MfeI C'AATT_G
Cuts at: 3792 3792
Size: 6193
MluI A'CGCG_T
Cuts at: 6069 6069
Size: 6193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2174 2355 2745 5307 5491 1305
Size: 869 181 390 2562 184 2007
Fragments arranged by size:
2562 2007 869 390 184 181
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3395 3488 10
Size: 54 1425 343 1563 93 2715
Fragments arranged by size:
2715 1563 1425 343 93 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2211 2339 2516 2570 2709 2996 4151
Size: 648 1045 128 177 54 139 287 1155
Cuts at: 4151 4510 4669 518
Size: 359 159 2042
Fragments arranged by size:
2042 1155 1045 648 359 287 177 159
139 128 54
MspA1I CmG'CkG
Cuts at: 1606 2232 3054 3085 3609 4286 5434 5679 1606
Size: 626 822 31 524 677 1148 245 2120
Fragments arranged by size:
2120 1148 822 677 626 524 245 31
NcoI C'CATG_G
Cuts at: 513 1065 2004 2517 513
Size: 552 939 513 4189
Fragments arranged by size:
4189 939 552 513
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6193
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6193
NotI GC'GGCC_GC
Cuts at: 3640 3640
Size: 6193
NsiI A_TGCA'T
Cuts at: 2070 2070
Size: 6193
NspI r_CATG'y
Cuts at: 1132 1229 3463 3551 5096 1132
Size: 97 2234 88 1545 2229
Fragments arranged by size:
2234 2229 1545 97 88
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6193
PciI A'CATG_T
Cuts at: 5092 5092
Size: 6193
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6193
PmeI GTTT'AAAC
Cuts at: 3497 3497
Size: 6193
PmlI CAC'GTG
Cuts at: 2190 2190
Size: 6193
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4262 4294 5775 5807 4262
Size: 32 1481 32 4648
Fragments arranged by size:
4648 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2829 2829
Size: 6193
PshAI GACnn'nnGTC
Cuts at: 1613 3385 1613
Size: 1772 4421
PsiI TTA'TAA
Cuts at: 3763 3763
Size: 6193
PstI C_TGCA'G
Cuts at: 830 3545 830
Size: 2715 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6193
PvuII CAG'CTG
Cuts at: 2232 3054 2232
Size: 822 5371
SacI G_AGCT'C
Cuts at: 2244 2244
Size: 6193
SalI G'TCGA_C
Cuts at: 1936 3535 1936
Size: 1599 4594
SanDI GG'GwC_CC
Cuts at: 2829 2829
Size: 6193
SapI GCTCTTCn'nnn_
Cuts at: 2249 2249
Size: 6193
SbfI CC_TGCA'GG
Cuts at: 3545 3545
Size: 6193
ScaI AGT'ACT
Cuts at: 4441 6153 4441
Size: 1712 4481
SfcI C'TryA_G
Cuts at: 826 1045 2485 3541 4676 5357 5548 826
Size: 219 1440 1056 1135 681 191 1471
Fragments arranged by size:
1471 1440 1135 1056 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6193
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6193
SmaI CCC'GGG
Cuts at: 1872 3520 1872
Size: 1648 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4297 5198 5460 820
Size: 197 926 2354 901 262 1553
Fragments arranged by size:
2354 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3984 493
Size: 3491 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6193
SphI G_CATG'C
Cuts at: 1132 3551 1132
Size: 2419 3774
SspI AAT'ATT
Cuts at: 5 52 4117 5
Size: 47 4065 2081
Fragments arranged by size:
4065 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6193
StyI C'CwwG_G
Cuts at: 513 1065 2004 2517 3632 513
Size: 552 939 513 1115 3074
Fragments arranged by size:
3074 1115 939 552 513
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2201 2294 2914 3434 4220 4237 4390
Size: 1255 621 93 620 520 786 17 153
Cuts at: 4390 4575 5020 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 786 621 620 520 445 185
153 93 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4439 6151 96
Size: 274 80 33 51 1443 2462 1712 138
Fragments arranged by size:
2462 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6153 6185 6153
Size: 32 6161
Tth111I GACn'n_nGTC
Cuts at: 1316 2267 1316
Size: 951 5242
XbaI T'CTAG_A
Cuts at: 3529 3529
Size: 6193
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2134 2268 2134
Size: 134 6059
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6193
XmaI C'CCGG_G
Cuts at: 1870 3518 1870
Size: 1648 4545
XmnI GAAnn'nnTTC
Cuts at: 4322 4322
Size: 6193
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4001 276
Size: 53 83 186 936 2467 2468
Fragments arranged by size:
2468 2467 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PspOMI PspXI PsrI RsrII SacII SexAI SfiI
SfoI SrfI SwaI