Restriction map for
pFN21AE9542
(Circular) (Six-base) MAPSORT of: pFN21AE9542.seq Check: 6278 from: 1 to: 6976
pFN21AE9542 6976 bp
With 182 enzymes: SgfI *
March 28, 2012 12:04 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4786 278
Size: 53 83 186 936 3250 2468
Fragments arranged by size:
3250 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4319 1937
Size: 2382 4594
Acc65I G'GTAC_C
Cuts at: 3980 4297 3980
Size: 317 6659
AclI AA'CG_TT
Cuts at: 1563 3889 5103 5476 1563
Size: 2326 1214 373 3063
Fragments arranged by size:
3063 2326 1214 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2387 2444 2591 2978 3947 5039 6423 6691
Size: 755 57 147 387 969 1092 1384 268
Cuts at: 6691 1632
Size: 1917
Fragments arranged by size:
1917 1384 1092 969 755 387 268 147
57
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6779
AflIII A'CryG_T
Cuts at: 1203 3157 5875 6852 1203
Size: 1954 2718 977 1327
Fragments arranged by size:
2718 1954 1327 977
AgeI A'CCGG_T
Cuts at: 5807 5807
Size: 6976
AhdI GACnn_n'nnGTC
Cuts at: 2075 5705 2075
Size: 3630 3346
AleI CACnn'nnGTG
Cuts at: 1166 2404 1166
Size: 1238 5738
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3277 1184
Size: 2093 4883
AlwNI CAG_nnn'CTG
Cuts at: 2137 2467 2893 3845 4719 6291 2137
Size: 330 426 952 874 1572 2822
Fragments arranged by size:
2822 1572 952 874 426 330
ApaLI G'TGCA_C
Cuts at: 5033 6189 5033
Size: 1156 5820
ApoI r'AATT_y
Cuts at: 1493 2640 2668 2794 3003 3066 3131 3189 3656
Size: 1147 28 126 209 63 65 58 467
Cuts at: 3656 3947 4285 4511 4663 6772 1493
Size: 291 338 226 152 2109 1697
Fragments arranged by size:
2109 1697 1147 467 338 291 226 209
152 126 65 63 58 28
AseI AT'TA_AT
Cuts at: 160 1032 2853 3765 5530 160
Size: 872 1821 912 1765 1606
Fragments arranged by size:
1821 1765 1606 912 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6976
AvaI C'yCGr_G
Cuts at: 1870 1943 4301 1870
Size: 73 2358 4545
Fragments arranged by size:
4545 2358 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3480 3513 3480
Size: 33 6943
BamHI G'GATC_C
Cuts at: 4306 4673 4306
Size: 367 6609
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3677 3980 4297 5752 618
Size: 325 217 627 1890 303 317 1455 1842
Fragments arranged by size:
1890 1842 1455 627 325 317 303 217
BanII G_rGCy'C
Cuts at: 1391 3733 4295 1391
Size: 2342 562 4072
Fragments arranged by size:
4072 2342 562
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2014 928
Size: 971 115 5890
Fragments arranged by size:
5890 971 115
BbvCI CC'TCA_GC
Cuts at: 2130 2130
Size: 6976
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5167 5201 1903
Size: 34 3230 34 3678
Fragments arranged by size:
3678 3230 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3921 4868 6078 3921
Size: 947 1210 4819
Fragments arranged by size:
4819 1210 947
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6976
BfrBI ATG'CAT
Cuts at: 3917 3917
Size: 6976
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5587 136
Size: 107 122 71 631 605 3915 1525
Fragments arranged by size:
3915 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2590 6971 2590
Size: 4381 2595
BlpI GC'TnA_GC
Cuts at: 4393 4393
Size: 6976
Bme1580I G_kGCm'C
Cuts at: 1790 5037 6193 1790
Size: 3247 1156 2573
Fragments arranged by size:
3247 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6976
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4707 5665 442
Size: 416 53 83 399 24 3290 958 1753
Fragments arranged by size:
3290 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6976
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2351 2870 5636 1239
Size: 1112 519 2766 2579
Fragments arranged by size:
2766 2579 1112 519
Bpu10I CC'TnA_GC
Cuts at: 2130 2130
Size: 6976
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3023 5101 5966 6264 3023
Size: 2078 865 298 3735
Fragments arranged by size:
3735 2078 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2214 3562 5639 882
Size: 617 715 1348 2077 2219
Fragments arranged by size:
2219 2077 1348 715 617
BsaAI yAC'GTr
Cuts at: 493 1204 4767 493
Size: 711 3563 2702
Fragments arranged by size:
3563 2702 711
BsaBI GATnn'nnATC
Cuts at: 6970 6970
Size: 6976
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4783 5165 5800 275
Size: 53 83 186 936 3250 382 635 1451
Fragments arranged by size:
3250 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5409 5807 6081 6228 6639 5409
Size: 398 274 147 411 5746
Fragments arranged by size:
5746 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4129 4159 1178
Size: 30 165 30 2726 30 3995
Fragments arranged by size:
3995 2726 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6976
BseYI C'CCAG_C
Cuts at: 6179 6675 6179
Size: 496 6480
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2123 1163
Size: 573 387 6016
Fragments arranged by size:
6016 573 387
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4426 5187 6215 1540
Size: 275 186 2425 761 1028 2301
Fragments arranged by size:
2425 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2087 5037 5122 6193 2087
Size: 2950 85 1071 2870
Fragments arranged by size:
2950 2870 1071 85
BsmI GAATG_Cn'
Cuts at: 4487 4580 4487
Size: 93 6883
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2087 3733 4295 5037 5122 6193 1391
Size: 399 297 1646 562 742 85 1071 2174
Fragments arranged by size:
2174 1646 1071 742 562 399 297 85
BspEI T'CCGG_A
Cuts at: 6639 6639
Size: 6976
BspHI T'CATG_A
Cuts at: 2452 4865 6595 2452
Size: 2413 1730 2833
Fragments arranged by size:
2833 2413 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2481 3269 4331 844
Size: 1637 788 1062 3489
Fragments arranged by size:
3489 1637 1062 788
BsrBI CCG'CTC
Cuts at: 1394 1823 4863 1394
Size: 429 3040 3507
Fragments arranged by size:
3507 3040 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2318 2400 5471 5645 65
Size: 1183 1070 82 3071 174 1396
Fragments arranged by size:
3071 1396 1183 1070 174 82
BsrFI r'CCGG_y
Cuts at: 1700 1810 5620 5807 1700
Size: 110 3810 187 2869
Fragments arranged by size:
3810 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6976
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6976
BssSI C'ACGA_G
Cuts at: 5036 6048 5036
Size: 1012 5964
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4186 794
Size: 3392 3584
BstBI TT'CG_AA
Cuts at: 5780 5780
Size: 6976
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6976
BstXI CCAn_nnnn'nTGG
Cuts at: 3143 3143
Size: 6976
BstYI r'GATC_y
Cuts at: 1972 2590 3470 4306 4673 5058 5075 6527 6613
Size: 618 880 836 367 385 17 1452 86
Cuts at: 6613 6625 6971 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 880 836 618 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6424
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6237
Fragments arranged by size:
6237 683 56
BtsI GCAGTG_nn'
Cuts at: 3956 4175 4492 5286 5306 3956
Size: 219 317 794 20 5626
Fragments arranged by size:
5626 794 317 219 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6976
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6941
DraI TTT'AAA
Cuts at: 4069 4280 4069
Size: 211 6765
DraIII CAC_nnn'GTG
Cuts at: 2121 2121
Size: 6976
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2395 5983 809
Size: 507 1079 3588 1802
Fragments arranged by size:
3588 1802 1079 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3477 3771 4172
Size: 54 1425 50 275 18 1647 294 401
Cuts at: 4172 4423 5312 6941 8
Size: 251 889 1629 43
Fragments arranged by size:
1647 1629 1425 889 401 294 275 251
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4423 1537
Size: 275 2611 4090
Fragments arranged by size:
4090 2611 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3705 4906 1353
Size: 2352 1201 3423
Fragments arranged by size:
3423 2352 1201
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5563 5937 6083 387
Size: 1064 138 3974 374 146 1280
Fragments arranged by size:
3974 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2351 2387 2444 2591 2870 2978 3947
Size: 393 719 36 57 147 279 108 969
Cuts at: 3947 5039 5636 6423 6691 1239
Size: 1092 597 787 268 1524
Fragments arranged by size:
1524 1092 969 787 719 597 393 279
268 147 108 57 36
EcoO109I rG'GnC_Cy
Cuts at: 2953 2953
Size: 6976
EcoRI G'AATT_C
Cuts at: 4285 4663 4285
Size: 378 6598
FspI TGC'GCA
Cuts at: 3432 5482 3432
Size: 2050 4926
HaeII r_GCGC'y
Cuts at: 1678 6123 1678
Size: 4445 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2061 2093 2914 2946 3222
Size: 32 135 32 489 32 821 32 276
Cuts at: 3222 3254 3285 3317 5617 5649 5691 5723 1373
Size: 32 31 32 2300 32 42 32 2626
Fragments arranged by size:
2626 2300 821 489 276 135 42 32
32 32 32 32 32 32 32 31
HincII GTy'rAC
Cuts at: 669 1657 1938 2735 3513 4320 4566 669
Size: 988 281 797 778 807 246 3079
Fragments arranged by size:
3079 988 807 797 778 281 246
HindIII A'AGCT_T
Cuts at: 748 2423 748
Size: 1675 5301
HpaI GTT'AAC
Cuts at: 3513 4566 3513
Size: 1053 5923
KpnI G_GTAC'C
Cuts at: 3984 4301 3984
Size: 317 6659
MfeI C'AATT_G
Cuts at: 2972 4575 2972
Size: 1603 5373
MluI A'CGCG_T
Cuts at: 6852 6852
Size: 6976
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3103 6090 6274 1305
Size: 1798 2987 184 2007
Fragments arranged by size:
2987 2007 1798 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3479 4174 10
Size: 54 1425 343 1647 695 2812
Fragments arranged by size:
2812 1647 1425 695 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2064 2284 2404 2862 3287 3828 4160
Size: 648 898 220 120 458 425 541 332
Cuts at: 4160 4934 5293 5452 518
Size: 774 359 159 2042
Fragments arranged by size:
2042 898 774 648 541 458 425 359
332 220 159 120
MspA1I CmG'CkG
Cuts at: 1606 3168 3436 4392 5069 6217 6462 1606
Size: 1562 268 956 677 1148 245 2120
Fragments arranged by size:
2120 1562 1148 956 677 268 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6424
NdeI CA'TA_TG
Cuts at: 387 4141 387
Size: 3754 3222
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6976
NotI GC'GGCC_GC
Cuts at: 4423 4423
Size: 6976
NsiI A_TGCA'T
Cuts at: 3919 3919
Size: 6976
NspI r_CATG'y
Cuts at: 1132 1229 2743 3161 3292 3431 3811 4334 5879
Size: 97 1514 418 131 139 380 523 1545
Cuts at: 5879 1132
Size: 2229
Fragments arranged by size:
2229 1545 1514 523 418 380 139 131
97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6976
PciI A'CATG_T
Cuts at: 3157 5875 3157
Size: 2718 4258
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2329 2893 1108
Size: 1221 564 5191
Fragments arranged by size:
5191 1221 564
PmeI GTTT'AAAC
Cuts at: 4280 4280
Size: 6976
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5045 5077 6558 6590 5045
Size: 32 1481 32 5431
Fragments arranged by size:
5431 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2953 2953
Size: 6976
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6976
PsiI TTA'TAA
Cuts at: 4546 4546
Size: 6976
PstI C_TGCA'G
Cuts at: 830 2816 3256 4182 4328 830
Size: 1986 440 926 146 3478
Fragments arranged by size:
3478 1986 926 440 146
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6976
PvuII CAG'CTG
Cuts at: 3168 3436 3168
Size: 268 6708
SalI G'TCGA_C
Cuts at: 1936 4318 1936
Size: 2382 4594
SbfI CC_TGCA'GG
Cuts at: 4328 4328
Size: 6976
ScaI AGT'ACT
Cuts at: 3140 3451 5224 6936 3140
Size: 311 1773 1712 3180
Fragments arranged by size:
3180 1773 1712 311
SexAI A'CCwGG_T
Cuts at: 3976 3976
Size: 6976
SfcI C'TryA_G
Cuts at: 826 1045 2812 3252 4099 4178 4324 5459 6140
Size: 219 1767 440 847 79 146 1135 681
Cuts at: 6140 6331 826
Size: 191 1471
Fragments arranged by size:
1767 1471 1135 847 681 440 219 191
146 79
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6976
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6976
SmaI CCC'GGG
Cuts at: 1872 4303 1872
Size: 2431 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3038 5080 5981 6243 820
Size: 197 926 1095 2042 901 262 1553
Fragments arranged by size:
2042 1553 1095 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4767 493
Size: 4274 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6976
SphI G_CATG'C
Cuts at: 1132 3292 3431 4334 1132
Size: 2160 139 903 3774
Fragments arranged by size:
3774 2160 903 139
SspI AAT'ATT
Cuts at: 5 52 2051 2228 4900 5
Size: 47 1999 177 2672 2081
Fragments arranged by size:
2672 2081 1999 177 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6976
StyI C'CwwG_G
Cuts at: 513 1065 2089 2323 4415 513
Size: 552 1024 234 2092 3074
Fragments arranged by size:
3074 2092 1024 552 234
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4150 5003 5020 5173 5358 5803 325
Size: 1255 2570 853 17 153 185 445 1498
Fragments arranged by size:
2570 1498 1255 853 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3138 3449 5222
Size: 274 80 33 51 1443 1161 311 1773
Cuts at: 5222 6934 96
Size: 1712 138
Fragments arranged by size:
1773 1712 1443 1161 311 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2066 2098 6936 6968 2066
Size: 32 4838 32 2074
Fragments arranged by size:
4838 2074 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6976
XbaI T'CTAG_A
Cuts at: 3199 4312 3199
Size: 1113 5863
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2751 4155 2751
Size: 1404 5572
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6976
XmaI C'CCGG_G
Cuts at: 1870 4301 1870
Size: 2431 4545
XmnI GAAnn'nnTTC
Cuts at: 2592 2931 4077 5105 2592
Size: 339 1146 1028 4463
Fragments arranged by size:
4463 1146 1028 339
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4784 276
Size: 53 83 186 936 3250 2468
Fragments arranged by size:
3250 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SfiI SfoI SrfI SwaI