Restriction map for
pFN21AE9541
(Circular) (Six-base) MAPSORT of: pFN21AE9541.seq Check: 1742 from: 1 to: 6049
pFN21AE9541 6049 bp
With 182 enzymes: SgfI *
March 28, 2012 12:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2052 2427 2052
Size: 375 5674
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3859 278
Size: 53 83 186 936 2323 2468
Fragments arranged by size:
2468 2323 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3333 3392 1937
Size: 1396 59 4594
Fragments arranged by size:
4594 1396 59
Acc65I G'GTAC_C
Cuts at: 3370 3370
Size: 6049
AclI AA'CG_TT
Cuts at: 1563 4176 4549 1563
Size: 2613 373 3063
Fragments arranged by size:
3063 2613 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2607 4112 5496 5764 1632
Size: 975 1505 1384 268 1917
Fragments arranged by size:
1917 1505 1384 975 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5852
AflIII A'CryG_T
Cuts at: 1203 3306 4948 5925 1203
Size: 2103 1642 977 1327
Fragments arranged by size:
2103 1642 1327 977
AgeI A'CCGG_T
Cuts at: 4880 4880
Size: 6049
AhdI GACnn_n'nnGTC
Cuts at: 4778 4778
Size: 6049
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6049
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2300 1184
Size: 1116 4933
AlwNI CAG_nnn'CTG
Cuts at: 2263 2696 2810 3792 5364 2263
Size: 433 114 982 1572 2948
Fragments arranged by size:
2948 1572 982 433 114
ApaLI G'TGCA_C
Cuts at: 4106 5262 4106
Size: 1156 4893
ApoI r'AATT_y
Cuts at: 1493 3358 3584 3736 5845 1493
Size: 1865 226 152 2109 1697
Fragments arranged by size:
2109 1865 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4603 160
Size: 872 3571 1606
Fragments arranged by size:
3571 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6049
AvaI C'yCGr_G
Cuts at: 1870 1943 2221 2513 3374 1870
Size: 73 278 292 861 4545
Fragments arranged by size:
4545 861 292 278 73
BamHI G'GATC_C
Cuts at: 2711 3379 3746 2711
Size: 668 367 5014
Fragments arranged by size:
5014 668 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2301 2540 2789 2840 2931
Size: 325 217 627 514 239 249 51 91
Cuts at: 2931 3113 3269 3275 3370 4825 618
Size: 182 156 6 95 1455 1842
Fragments arranged by size:
1842 1455 627 514 325 249 239 217
182 156 95 91 51 6
BanII G_rGCy'C
Cuts at: 1391 2072 2690 3095 3260 3368 1391
Size: 681 618 405 165 108 4072
Fragments arranged by size:
4072 681 618 405 165 108
BbeI G_GCGC'C
Cuts at: 2935 2935
Size: 6049
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5078
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4240 4274 1903
Size: 34 2303 34 3678
Fragments arranged by size:
3678 2303 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3941 5151 3941
Size: 1210 4839
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6049
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2288 2318 2929
Size: 107 122 71 631 605 616 30 611
Cuts at: 2929 4660 136
Size: 1731 1525
Fragments arranged by size:
1731 1525 631 616 611 605 122 107
71 30
BglII A'GATC_T
Cuts at: 6044 6044
Size: 6049
BlpI GC'TnA_GC
Cuts at: 3466 3466
Size: 6049
Bme1580I G_kGCm'C
Cuts at: 1790 2279 2470 2522 2602 3116 4110 5266 1790
Size: 489 191 52 80 514 994 1156 2573
Fragments arranged by size:
2573 1156 994 514 489 191 80 52
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6049
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2309 2717 3780
Size: 416 53 83 399 24 892 408 1063
Cuts at: 3780 4738 442
Size: 958 1753
Fragments arranged by size:
1753 1063 958 892 416 408 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6049
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4709 1239
Size: 3470 2579
Bpu10I CC'TnA_GC
Cuts at: 2411 2568 2689 3089 2411
Size: 157 121 400 5371
Fragments arranged by size:
5371 400 157 121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4174 5039 5337 4174
Size: 865 298 4886
Fragments arranged by size:
4886 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2465 2648 4712 882
Size: 617 966 183 2064 2219
Fragments arranged by size:
2219 2064 966 617 183
BsaAI yAC'GTr
Cuts at: 493 1204 2308 3307 3840 493
Size: 711 1104 999 533 2702
Fragments arranged by size:
2702 1104 999 711 533
BsaBI GATnn'nnATC
Cuts at: 6043 6043
Size: 6049
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2932 3856 4238 4873
Size: 53 83 186 936 1399 924 382 635
Cuts at: 4873 275
Size: 1451
Fragments arranged by size:
1451 1399 936 924 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4482 4880 5154 5301 5712 4482
Size: 398 274 147 411 4819
Fragments arranged by size:
4819 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5824
Fragments arranged by size:
5824 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2741 1186
Size: 1555 4494
BseYI C'CCAG_C
Cuts at: 2020 2173 2851 2959 3046 5252 5748 2020
Size: 153 678 108 87 2206 496 2321
Fragments arranged by size:
2321 2206 678 496 153 108 87
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2924 3031 1163
Size: 573 1188 107 4181
Fragments arranged by size:
4181 1188 573 107
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3221 3499 4260 5288 1540
Size: 275 186 1220 278 761 1028 2301
Fragments arranged by size:
2301 1220 1028 761 278 275 186
BsiHKAI G_wGCw'C
Cuts at: 4110 4195 5266 4110
Size: 85 1071 4893
Fragments arranged by size:
4893 1071 85
BsmI GAATG_Cn'
Cuts at: 3560 3653 3560
Size: 93 5956
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2072 2279 2470 2522 2602 2690 3095
Size: 399 282 207 191 52 80 88 405
Cuts at: 3095 3116 3260 3368 4110 4195 5266 1391
Size: 21 144 108 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 405 399 282 207 191
144 108 88 85 80 52 21
BspEI T'CCGG_A
Cuts at: 5712 5712
Size: 6049
BspHI T'CATG_A
Cuts at: 3938 5668 3938
Size: 1730 4319
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2052 2227 2427 3003 3404 844
Size: 1208 175 200 576 401 3489
Fragments arranged by size:
3489 1208 576 401 200 175
BsrBI CCG'CTC
Cuts at: 1394 1823 3936 1394
Size: 429 2113 3507
Fragments arranged by size:
3507 2113 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2376 2759 3224 4544 4718 65
Size: 1183 1128 383 465 1320 174 1396
Fragments arranged by size:
1396 1320 1183 1128 465 383 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3220 3272 4693 4880 1700
Size: 110 1410 52 1421 187 2869
Fragments arranged by size:
2869 1421 1410 187 110 52
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6049
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6049
BssSI C'ACGA_G
Cuts at: 4109 5121 4109
Size: 1012 5037
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2606 794
Size: 1812 4237
BstBI TT'CG_AA
Cuts at: 4853 4853
Size: 6049
BstEII G'GTnAC_C
Cuts at: 1184 2197 1184
Size: 1013 5036
BstXI CCAn_nnnn'nTGG
Cuts at: 2136 2251 3253 2136
Size: 115 1002 4932
Fragments arranged by size:
4932 1002 115
BstYI r'GATC_y
Cuts at: 1972 2091 2711 3379 3746 4131 4148 5600 5686
Size: 119 620 668 367 385 17 1452 86
Cuts at: 5686 5698 6044 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 668 620 385 367 346 119
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2811 2811
Size: 6049
BtgI C'CryG_G
Cuts at: 513 1065 2004 2836 2997 513
Size: 552 939 832 161 3565
Fragments arranged by size:
3565 939 832 552 161
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5310
Fragments arranged by size:
5310 683 56
BtsI GCAGTG_nn'
Cuts at: 2971 3117 3565 4359 4379 2971
Size: 146 448 794 20 4641
Fragments arranged by size:
4641 794 448 146 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6049
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6014
DraI TTT'AAA
Cuts at: 3353 3353
Size: 6049
DraIII CAC_nnn'GTG
Cuts at: 2308 2338 2308
Size: 30 6019
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2813 5056 809
Size: 507 1497 2243 1802
Fragments arranged by size:
2243 1802 1497 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2994 3218 3243
Size: 54 1425 50 275 18 1164 224 25
Cuts at: 3243 3496 4385 6014 8
Size: 253 889 1629 43
Fragments arranged by size:
1629 1425 1164 889 275 253 224 54
50 43 25 18
EagI C'GGCC_G
Cuts at: 1537 1812 3218 3496 1537
Size: 275 1406 278 4090
Fragments arranged by size:
4090 1406 278 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2727 2742 3979 1353
Size: 1374 15 1237 3423
Fragments arranged by size:
3423 1374 1237 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4636 5010 5156 387
Size: 1064 138 3047 374 146 1280
Fragments arranged by size:
3047 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2607 4112 4709 5496 5764 1239
Size: 393 975 1505 597 787 268 1524
Fragments arranged by size:
1524 1505 975 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2129 2453 2129
Size: 324 5725
EcoO109I rG'GnC_Cy
Cuts at: 2239 2693 3015 3063 2239
Size: 454 322 48 5225
Fragments arranged by size:
5225 454 322 48
EcoRI G'AATT_C
Cuts at: 3358 3736 3358
Size: 378 5671
FspI TGC'GCA
Cuts at: 4555 4555
Size: 6049
HaeII r_GCGC'y
Cuts at: 1678 2174 2531 2935 5196 1678
Size: 496 357 404 2261 2531
Fragments arranged by size:
2531 2261 496 404 357
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4690 4722 4764 4796 1373
Size: 32 135 32 3118 32 42 32 2626
Fragments arranged by size:
3118 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3393 3639 669
Size: 988 281 1455 246 3079
Fragments arranged by size:
3079 1455 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6049
HpaI GTT'AAC
Cuts at: 3639 3639
Size: 6049
KasI G'GCGC_C
Cuts at: 2931 2931
Size: 6049
KpnI G_GTAC'C
Cuts at: 3374 3374
Size: 6049
MfeI C'AATT_G
Cuts at: 3648 3648
Size: 6049
MluI A'CGCG_T
Cuts at: 5925 5925
Size: 6049
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2117 5163 5347 1305
Size: 812 3046 184 2007
Fragments arranged by size:
3046 2007 812 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2996 3245 10
Size: 54 1425 343 1164 249 2814
Fragments arranged by size:
2814 1425 1164 343 249 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2249 3002 3251 4007 4366 4525 518
Size: 648 1083 753 249 756 359 159 2042
Fragments arranged by size:
2042 1083 756 753 648 359 249 159
MspA1I CmG'CkG
Cuts at: 1606 2627 2824 3236 3465 4142 5290 5535 1606
Size: 1021 197 412 229 677 1148 245 2120
Fragments arranged by size:
2120 1148 1021 677 412 245 229 197
NaeI GCC'GGC
Cuts at: 3222 3222
Size: 6049
NarI GG'CG_CC
Cuts at: 2932 2932
Size: 6049
NcoI C'CATG_G
Cuts at: 513 1065 2004 2836 2997 513
Size: 552 939 832 161 3565
Fragments arranged by size:
3565 939 832 552 161
NdeI CA'TA_TG
Cuts at: 387 3175 387
Size: 2788 3261
NgoMIV G'CCGG_C
Cuts at: 3220 3220
Size: 6049
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6049
NotI GC'GGCC_GC
Cuts at: 3496 3496
Size: 6049
NspI r_CATG'y
Cuts at: 1132 1229 3055 3407 4952 1132
Size: 97 1826 352 1545 2229
Fragments arranged by size:
2229 1826 1545 352 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6049
PciI A'CATG_T
Cuts at: 4948 4948
Size: 6049
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2047 2338 1108
Size: 939 291 4819
Fragments arranged by size:
4819 939 291
PmeI GTTT'AAAC
Cuts at: 3353 3353
Size: 6049
PmlI CAC'GTG
Cuts at: 2308 3307 2308
Size: 999 5050
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4118 4150 5631 5663 4118
Size: 32 1481 32 4504
Fragments arranged by size:
4504 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2239 3015 2239
Size: 776 5273
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6049
PsiI TTA'TAA
Cuts at: 3619 3619
Size: 6049
PspXI vC'TCGA_Gb
Cuts at: 2513 2513
Size: 6049
PstI C_TGCA'G
Cuts at: 830 3124 3401 830
Size: 2294 277 3478
Fragments arranged by size:
3478 2294 277
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6049
PvuII CAG'CTG
Cuts at: 2627 2824 3236 2627
Size: 197 412 5440
Fragments arranged by size:
5440 412 197
SalI G'TCGA_C
Cuts at: 1936 3391 1936
Size: 1455 4594
SbfI CC_TGCA'GG
Cuts at: 3401 3401
Size: 6049
ScaI AGT'ACT
Cuts at: 4297 6009 4297
Size: 1712 4337
SfcI C'TryA_G
Cuts at: 826 1045 3120 3397 4532 5213 5404 826
Size: 219 2075 277 1135 681 191 1471
Fragments arranged by size:
2075 1471 1135 681 277 219 191
SfoI GGC'GCC
Cuts at: 2933 2933
Size: 6049
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6049
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6049
SmaI CCC'GGG
Cuts at: 1872 2223 3376 1872
Size: 351 1153 4545
Fragments arranged by size:
4545 1153 351
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2513 4153 5054 5316 820
Size: 197 926 570 1640 901 262 1553
Fragments arranged by size:
1640 1553 926 901 570 262 197
SnaBI TAC'GTA
Cuts at: 493 3840 493
Size: 3347 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6049
SphI G_CATG'C
Cuts at: 1132 3055 3407 1132
Size: 1923 352 3774
Fragments arranged by size:
3774 1923 352
SspI AAT'ATT
Cuts at: 5 52 3973 5
Size: 47 3921 2081
Fragments arranged by size:
3921 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6049
StyI C'CwwG_G
Cuts at: 513 1065 2004 2358 2836 2997 3488 513
Size: 552 939 354 478 161 491 3074
Fragments arranged by size:
3074 939 552 491 478 354 161
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2029 2086 3139 4076 4093 4246 4431
Size: 1255 449 57 1053 937 17 153 185
Cuts at: 4431 4876 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1053 937 449 445 185 153
57 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4295 6007 96
Size: 274 80 33 51 1443 2318 1712 138
Fragments arranged by size:
2318 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3069 3101 6009 6041 3069
Size: 32 2908 32 3077
Fragments arranged by size:
3077 2908 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6049
XbaI T'CTAG_A
Cuts at: 3385 3385
Size: 6049
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3124 3124
Size: 6049
XhoI C'TCGA_G
Cuts at: 1943 2513 1943
Size: 570 5479
XmaI C'CCGG_G
Cuts at: 1870 2221 3374 1870
Size: 351 1153 4545
Fragments arranged by size:
4545 1153 351
XmnI GAAnn'nnTTC
Cuts at: 4178 4178
Size: 6049
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3857 276
Size: 53 83 186 936 2323 2468
Fragments arranged by size:
2468 2323 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI
FspAI NruI NsiI PacI PfoI PspOMI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SrfI SwaI