Restriction map for
pFN21AE9537
(Circular) (Six-base) MAPSORT of: pFN21AE9537.seq Check: 1343 from: 1 to: 5677
pFN21AE9537 5677 bp
With 182 enzymes: SgfI *
December 28, 2011 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3487 278
Size: 53 83 186 936 1951 2468
Fragments arranged by size:
2468 1951 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3020 1937
Size: 1083 4594
Acc65I G'GTAC_C
Cuts at: 2998 2998
Size: 5677
AclI AA'CG_TT
Cuts at: 1563 3804 4177 1563
Size: 2241 373 3063
Fragments arranged by size:
3063 2241 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2237 2682 2784 3740 5124 5392 1632
Size: 605 445 102 956 1384 268 1917
Fragments arranged by size:
1917 1384 956 605 445 268 102
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5480
AflIII A'CryG_T
Cuts at: 1203 2053 4576 5553 1203
Size: 850 2523 977 1327
Fragments arranged by size:
2523 1327 977 850
AgeI A'CCGG_T
Cuts at: 2974 4508 2974
Size: 1534 4143
AhdI GACnn_n'nnGTC
Cuts at: 4406 4406
Size: 5677
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5677
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5677
AlwNI CAG_nnn'CTG
Cuts at: 2021 2453 2705 2816 3420 4992 2021
Size: 432 252 111 604 1572 2706
Fragments arranged by size:
2706 1572 604 432 252 111
ApaI G_GGCC'C
Cuts at: 2508 2508
Size: 5677
ApaLI G'TGCA_C
Cuts at: 3734 4890 3734
Size: 1156 4521
ApoI r'AATT_y
Cuts at: 1493 2857 2986 3212 3364 5473 1493
Size: 1364 129 226 152 2109 1697
Fragments arranged by size:
2109 1697 1364 226 152 129
AseI AT'TA_AT
Cuts at: 160 1032 4231 160
Size: 872 3199 1606
Fragments arranged by size:
3199 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5677
AvaI C'yCGr_G
Cuts at: 1870 1943 2241 3002 1870
Size: 73 298 761 4545
Fragments arranged by size:
4545 761 298 73
BamHI G'GATC_C
Cuts at: 3007 3374 3007
Size: 367 5310
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2090 2998 4453 618
Size: 325 217 627 303 908 1455 1842
Fragments arranged by size:
1842 1455 908 627 325 303 217
BanII G_rGCy'C
Cuts at: 1391 2469 2508 2996 1391
Size: 1078 39 488 4072
Fragments arranged by size:
4072 1078 488 39
BbeI G_GCGC'C
Cuts at: 2094 2094
Size: 5677
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2077 928
Size: 971 178 4528
Fragments arranged by size:
4528 971 178
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3868 3902 1903
Size: 34 1931 34 3678
Fragments arranged by size:
3678 1931 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3569 4779 3569
Size: 1210 4467
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5677
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4288 136
Size: 107 122 71 631 605 2616 1525
Fragments arranged by size:
2616 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5672 5672
Size: 5677
BlpI GC'TnA_GC
Cuts at: 2025 3094 2025
Size: 1069 4608
Bme1580I G_kGCm'C
Cuts at: 1790 2508 3738 4894 1790
Size: 718 1230 1156 2573
Fragments arranged by size:
2573 1230 1156 718
BmgBI CAC'GTC
Cuts at: 1318 2056 1318
Size: 738 4939
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2329 2431 3408
Size: 416 53 83 399 24 912 102 977
Cuts at: 3408 4366 442
Size: 958 1753
Fragments arranged by size:
1753 977 958 912 416 399 102 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5677
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2135 2135
Size: 5677
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4337 1239
Size: 3098 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2165 3802 4667 4965 2165
Size: 1637 865 298 2877
Fragments arranged by size:
2877 1637 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4340 882
Size: 617 2841 2219
Fragments arranged by size:
2841 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3468 493
Size: 711 2264 2702
Fragments arranged by size:
2702 2264 711
BsaBI GATnn'nnATC
Cuts at: 5671 5671
Size: 5677
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2091 3484 3866 4501
Size: 53 83 186 936 558 1393 382 635
Cuts at: 4501 275
Size: 1451
Fragments arranged by size:
1451 1393 936 635 558 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2974 4110 4508 4782 4929 5340 2974
Size: 1136 398 274 147 411 3311
Fragments arranged by size:
3311 1136 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2241 2271 1178
Size: 30 165 30 838 30 4584
Fragments arranged by size:
4584 838 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5677
BseYI C'CCAG_C
Cuts at: 4880 5376 4880
Size: 496 5181
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2283 2829 1163
Size: 573 547 546 4011
Fragments arranged by size:
4011 573 547 546
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2299 2712 3127 3888 4916 1540
Size: 275 186 298 413 415 761 1028 2301
Fragments arranged by size:
2301 1028 761 415 413 298 275 186
BsiHKAI G_wGCw'C
Cuts at: 2181 3738 3823 4894 2181
Size: 1557 85 1071 2964
Fragments arranged by size:
2964 1557 1071 85
BsmI GAATG_Cn'
Cuts at: 2578 3188 3281 2578
Size: 610 93 4974
Fragments arranged by size:
4974 610 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2181 2469 2508 2996 3738 3823 4894
Size: 399 391 288 39 488 742 85 1071
Cuts at: 4894 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 488 399 391 288 85
39
BspEI T'CCGG_A
Cuts at: 5340 5340
Size: 5677
BspHI T'CATG_A
Cuts at: 3566 5296 3566
Size: 1730 3947
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3032 844
Size: 2188 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2238 3564 1394
Size: 429 415 1326 3507
Fragments arranged by size:
3507 1326 429 415
BsrDI GCAATG_nn'
Cuts at: 65 1248 4172 4346 65
Size: 1183 2924 174 1396
Fragments arranged by size:
2924 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2974 4321 4508 1700
Size: 110 1164 1347 187 2869
Fragments arranged by size:
2869 1347 1164 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5677
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5677
BssSI C'ACGA_G
Cuts at: 3737 4749 3737
Size: 1012 4665
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2021 794
Size: 1227 4450
BstBI TT'CG_AA
Cuts at: 4481 4481
Size: 5677
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5677
BstYI r'GATC_y
Cuts at: 1972 2610 3007 3374 3759 3776 5228 5314 5326
Size: 638 397 367 385 17 1452 86 12
Cuts at: 5326 5672 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 638 397 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2172 2205 513
Size: 552 1107 33 3985
Fragments arranged by size:
3985 1107 552 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4938
Fragments arranged by size:
4938 683 56
BtsI GCAGTG_nn'
Cuts at: 3193 3987 4007 3193
Size: 794 20 4863
Fragments arranged by size:
4863 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5677
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5642
DraI TTT'AAA
Cuts at: 2981 2981
Size: 5677
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2054 4684 809
Size: 507 738 2630 1802
Fragments arranged by size:
2630 1802 738 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2578 3124 4013
Size: 54 1425 50 275 18 748 546 889
Cuts at: 4013 5642 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 748 546 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3124 1537
Size: 275 1312 4090
Fragments arranged by size:
4090 1312 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2552 2595 3607 1353
Size: 1199 43 1012 3423
Fragments arranged by size:
3423 1199 1012 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4264 4638 4784 387
Size: 1064 138 2675 374 146 1280
Fragments arranged by size:
2675 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2237 2682 2784 3740 4337 5124 5392
Size: 393 605 445 102 956 597 787 268
Cuts at: 5392 1239
Size: 1524
Fragments arranged by size:
1524 956 787 605 597 445 393 268
102
EcoO109I rG'GnC_Cy
Cuts at: 2192 2401 2504 2505 2790 2192
Size: 209 103 1 285 5079
Fragments arranged by size:
5079 285 209 103 1
EcoRI G'AATT_C
Cuts at: 2857 2986 3364 2857
Size: 129 378 5170
Fragments arranged by size:
5170 378 129
FspI TGC'GCA
Cuts at: 4183 4183
Size: 5677
HaeII r_GCGC'y
Cuts at: 1678 2094 4824 1678
Size: 416 2730 2531
Fragments arranged by size:
2730 2531 416
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2292 2324 2816 2848 4318
Size: 32 135 32 720 32 492 32 1470
Cuts at: 4318 4350 4392 4424 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1470 720 492 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3021 3267 669
Size: 988 281 1083 246 3079
Fragments arranged by size:
3079 1083 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5677
HpaI GTT'AAC
Cuts at: 3267 3267
Size: 5677
KasI G'GCGC_C
Cuts at: 2090 2090
Size: 5677
KpnI G_GTAC'C
Cuts at: 3002 3002
Size: 5677
MfeI C'AATT_G
Cuts at: 3276 3276
Size: 5677
MluI A'CGCG_T
Cuts at: 5553 5553
Size: 5677
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2442 4791 4975 1305
Size: 1137 2349 184 2007
Fragments arranged by size:
2349 2007 1137 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2580 10
Size: 54 1425 343 748 3107
Fragments arranged by size:
3107 1425 748 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3635 3994 4153 518
Size: 648 2469 359 159 2042
Fragments arranged by size:
2469 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2018 3093 3770 4918 5163 1606
Size: 412 1075 677 1148 245 2120
Fragments arranged by size:
2120 1148 1075 677 412 245
NarI GG'CG_CC
Cuts at: 2091 2091
Size: 5677
NcoI C'CATG_G
Cuts at: 513 1065 2172 513
Size: 552 1107 4018
Fragments arranged by size:
4018 1107 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5677
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5677
NotI GC'GGCC_GC
Cuts at: 3124 3124
Size: 5677
NspI r_CATG'y
Cuts at: 1132 1229 2145 2202 2377 3035 4580 1132
Size: 97 916 57 175 658 1545 2229
Fragments arranged by size:
2229 1545 916 658 175 97 57
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5677
PciI A'CATG_T
Cuts at: 4576 4576
Size: 5677
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2620 1108
Size: 1512 4165
PfoI T'CCnGG_A
Cuts at: 2722 2722
Size: 5677
PmeI GTTT'AAAC
Cuts at: 2981 2981
Size: 5677
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3746 3778 5259 5291 3746
Size: 32 1481 32 4132
Fragments arranged by size:
4132 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2401 2790 2401
Size: 389 5288
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5677
PsiI TTA'TAA
Cuts at: 3247 3247
Size: 5677
PspOMI G'GGCC_C
Cuts at: 2504 2504
Size: 5677
PstI C_TGCA'G
Cuts at: 830 2017 2274 2496 2938 3029 830
Size: 1187 257 222 442 91 3478
Fragments arranged by size:
3478 1187 442 257 222 91
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5677
PvuII CAG'CTG
Cuts at: 2018 2018
Size: 5677
SalI G'TCGA_C
Cuts at: 1936 3019 1936
Size: 1083 4594
SanDI GG'GwC_CC
Cuts at: 2401 2401
Size: 5677
SbfI CC_TGCA'GG
Cuts at: 3029 3029
Size: 5677
ScaI AGT'ACT
Cuts at: 3925 5637 3925
Size: 1712 3965
SfcI C'TryA_G
Cuts at: 826 1045 2013 2270 2492 2640 2934 3025 4160
Size: 219 968 257 222 148 294 91 1135
Cuts at: 4160 4841 5032 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 968 681 294 257 222 219
191 148 91
SfoI GGC'GCC
Cuts at: 2092 2092
Size: 5677
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5677
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5677
SmaI CCC'GGG
Cuts at: 1872 2243 3004 1872
Size: 371 761 4545
Fragments arranged by size:
4545 761 371
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2180 3781 4682 4944 820
Size: 197 926 237 1601 901 262 1553
Fragments arranged by size:
1601 1553 926 901 262 237 197
SnaBI TAC'GTA
Cuts at: 493 3468 493
Size: 2975 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5677
SphI G_CATG'C
Cuts at: 1132 2145 2377 3035 1132
Size: 1013 232 658 3774
Fragments arranged by size:
3774 1013 658 232
SspI AAT'ATT
Cuts at: 5 52 2661 3601 5
Size: 47 2609 940 2081
Fragments arranged by size:
2609 2081 940 47
StuI AGG'CCT
Cuts at: 1517 2873 1517
Size: 1356 4321
StyI C'CwwG_G
Cuts at: 513 1065 2172 3116 513
Size: 552 1107 944 3074
Fragments arranged by size:
3074 1107 944 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2313 3704 3721 3874 4059 4504 325
Size: 1255 733 1391 17 153 185 445 1498
Fragments arranged by size:
1498 1391 1255 733 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3923 5635 96
Size: 274 80 33 51 1443 1946 1712 138
Fragments arranged by size:
1946 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5637 5669 5637
Size: 32 5645
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5677
XbaI T'CTAG_A
Cuts at: 3013 3013
Size: 5677
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5677
XmaI C'CCGG_G
Cuts at: 1870 2241 3002 1870
Size: 371 761 4545
Fragments arranged by size:
4545 761 371
XmnI GAAnn'nnTTC
Cuts at: 3806 3806
Size: 5677
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3485 276
Size: 53 83 186 936 1951 2468
Fragments arranged by size:
2468 1951 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI RsrII SacI SacII
SapI SexAI SfiI SrfI SwaI XcmI