Restriction map for
pFN21AE9535
(Circular) (Six-base) MAPSORT of: pFN21AE9535.seq Check: 1249 from: 1 to: 6802
pFN21AE9535 6802 bp
With 182 enzymes: SgfI *
December 28, 2011 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3752 3752
Size: 6802
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4612 278
Size: 53 83 186 936 3076 2468
Fragments arranged by size:
3076 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3366 4145 1937
Size: 1429 779 4594
Fragments arranged by size:
4594 1429 779
Acc65I G'GTAC_C
Cuts at: 4123 4123
Size: 6802
AclI AA'CG_TT
Cuts at: 1563 4929 5302 1563
Size: 3366 373 3063
Fragments arranged by size:
3366 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2057 4865 6249 6517 1632
Size: 425 2808 1384 268 1917
Fragments arranged by size:
2808 1917 1384 425 268
AfeI AGC'GCT
Cuts at: 2076 2076
Size: 6802
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6605
AflIII A'CryG_T
Cuts at: 1203 5701 6678 1203
Size: 4498 977 1327
Fragments arranged by size:
4498 1327 977
AgeI A'CCGG_T
Cuts at: 5633 5633
Size: 6802
AhdI GACnn_n'nnGTC
Cuts at: 5531 5531
Size: 6802
AleI CACnn'nnGTG
Cuts at: 1166 2841 1166
Size: 1675 5127
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2129 3238 1184
Size: 945 1109 4748
Fragments arranged by size:
4748 1109 945
AlwNI CAG_nnn'CTG
Cuts at: 2035 2713 2838 4545 6117 2035
Size: 678 125 1707 1572 2720
Fragments arranged by size:
2720 1707 1572 678 125
ApaLI G'TGCA_C
Cuts at: 4859 6015 4859
Size: 1156 5646
ApoI r'AATT_y
Cuts at: 1493 2452 2466 2875 3648 3923 4111 4337 4489
Size: 959 14 409 773 275 188 226 152
Cuts at: 4489 6598 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 959 773 409 275 226 188
152 14
AseI AT'TA_AT
Cuts at: 160 1032 3111 3677 5356 160
Size: 872 2079 566 1679 1606
Fragments arranged by size:
2079 1679 1606 872 566
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6802
AvaI C'yCGr_G
Cuts at: 1870 1943 4127 1870
Size: 73 2184 4545
Fragments arranged by size:
4545 2184 73
BamHI G'GATC_C
Cuts at: 4132 4499 4132
Size: 367 6435
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2123 3751 4123 5578 618
Size: 325 217 627 336 1628 372 1455 1842
Fragments arranged by size:
1842 1628 1455 627 372 336 325 217
BanII G_rGCy'C
Cuts at: 1391 2103 2352 3234 4121 1391
Size: 712 249 882 887 4072
Fragments arranged by size:
4072 887 882 712 249
BbeI G_GCGC'C
Cuts at: 2127 2127
Size: 6802
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2428 928
Size: 971 529 5302
Fragments arranged by size:
5302 971 529
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4993 5027 1903
Size: 34 3056 34 3678
Fragments arranged by size:
3678 3056 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4694 5904 4694
Size: 1210 5592
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6802
BfrBI ATG'CAT
Cuts at: 2582 2582
Size: 6802
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5413 136
Size: 107 122 71 631 605 3741 1525
Fragments arranged by size:
3741 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6797 6797
Size: 6802
BlpI GC'TnA_GC
Cuts at: 3917 4219 3917
Size: 302 6500
Bme1580I G_kGCm'C
Cuts at: 1790 2118 4863 6019 1790
Size: 328 2745 1156 2573
Fragments arranged by size:
2745 2573 1156 328
BmgBI CAC'GTC
Cuts at: 1318 2757 1318
Size: 1439 5363
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4533 5491 442
Size: 416 53 83 399 24 3116 958 1753
Fragments arranged by size:
3116 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6802
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3494 3494
Size: 6802
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2630 2654 2831 5462 1239
Size: 1391 24 177 2631 2579
Fragments arranged by size:
2631 2579 1391 177 24
Bpu10I CC'TnA_GC
Cuts at: 2097 2097
Size: 6802
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2187 2407 4927 5792 6090 2187
Size: 220 2520 865 298 2899
Fragments arranged by size:
2899 2520 865 298 220
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2159 5465 882
Size: 617 660 3306 2219
Fragments arranged by size:
3306 2219 660 617
BsaAI yAC'GTr
Cuts at: 493 1204 4593 493
Size: 711 3389 2702
Fragments arranged by size:
3389 2702 711
BsaBI GATnn'nnATC
Cuts at: 6796 6796
Size: 6802
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2124 4609 4991 5626
Size: 53 83 186 936 591 2485 382 635
Cuts at: 5626 275
Size: 1451
Fragments arranged by size:
2485 1451 936 635 591 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5235 5633 5907 6054 6465 5235
Size: 398 274 147 411 5572
Fragments arranged by size:
5572 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2804 2834 1178
Size: 30 165 30 1401 30 5146
Fragments arranged by size:
5146 1401 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2285 2431 1186
Size: 1099 146 5557
Fragments arranged by size:
5557 1099 146
BseYI C'CCAG_C
Cuts at: 2831 2988 6005 6501 2831
Size: 157 3017 496 3132
Fragments arranged by size:
3132 3017 496 157
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3340 1163
Size: 573 1604 4625
Fragments arranged by size:
4625 1604 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2151 4252 5013 6041 1540
Size: 275 186 150 2101 761 1028 2301
Fragments arranged by size:
2301 2101 1028 761 275 186 150
BsiHKAI G_wGCw'C
Cuts at: 3037 4863 4948 6019 3037
Size: 1826 85 1071 3820
Fragments arranged by size:
3820 1826 1071 85
BsmI GAATG_Cn'
Cuts at: 2582 4313 4406 2582
Size: 1731 93 4978
Fragments arranged by size:
4978 1731 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2103 2118 2352 3037 3234 4121 4863
Size: 399 313 15 234 685 197 887 742
Cuts at: 4863 4948 6019 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 887 742 685 399 313 234
197 85 15
BspEI T'CCGG_A
Cuts at: 6465 6465
Size: 6802
BspHI T'CATG_A
Cuts at: 4691 6421 4691
Size: 1730 5072
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3752 4157 844
Size: 2908 405 3489
Fragments arranged by size:
3489 2908 405
BsrBI CCG'CTC
Cuts at: 1394 1823 4689 1394
Size: 429 2866 3507
Fragments arranged by size:
3507 2866 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4049 5297 5471 65
Size: 1183 2801 1248 174 1396
Fragments arranged by size:
2801 1396 1248 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2150 5446 5633 1700
Size: 110 340 3296 187 2869
Fragments arranged by size:
3296 2869 340 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6802
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6802
BssSI C'ACGA_G
Cuts at: 2070 4862 5874 2070
Size: 2792 1012 2998
Fragments arranged by size:
2998 2792 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2713 794
Size: 1919 4883
BstBI TT'CG_AA
Cuts at: 3664 5606 3664
Size: 1942 4860
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6802
BstXI CCAn_nnnn'nTGG
Cuts at: 3559 3559
Size: 6802
BstYI r'GATC_y
Cuts at: 1972 4132 4499 4884 4901 6353 6439 6451 6797
Size: 2160 367 385 17 1452 86 12 346
Cuts at: 6797 1972
Size: 1977
Fragments arranged by size:
2160 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3089 3089
Size: 6802
BtgI C'CryG_G
Cuts at: 513 1065 2145 2659 3552 513
Size: 552 1080 514 893 3763
Fragments arranged by size:
3763 1080 893 552 514
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6063
Fragments arranged by size:
6063 683 56
BtsI GCAGTG_nn'
Cuts at: 2836 3324 4318 5112 5132 2836
Size: 488 994 794 20 4506
Fragments arranged by size:
4506 994 794 488 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6802
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6767
DraI TTT'AAA
Cuts at: 4106 4106
Size: 6802
DraIII CAC_nnn'GTG
Cuts at: 4098 4098
Size: 6802
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5809 809
Size: 507 4493 1802
Fragments arranged by size:
4493 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2148 4249 5138
Size: 54 1425 50 275 18 318 2101 889
Cuts at: 5138 6767 8
Size: 1629 43
Fragments arranged by size:
2101 1629 1425 889 318 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2148 4249 1537
Size: 275 336 2101 4090
Fragments arranged by size:
4090 2101 336 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4732 1353
Size: 3379 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5389 5763 5909 387
Size: 1064 138 3800 374 146 1280
Fragments arranged by size:
3800 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2057 2630 2654 2831 4865 5462 6249
Size: 393 425 573 24 177 2034 597 787
Cuts at: 6249 6517 1239
Size: 268 1524
Fragments arranged by size:
2034 1524 787 597 573 425 393 268
177 24
EcoO109I rG'GnC_Cy
Cuts at: 2710 3094 2710
Size: 384 6418
EcoRI G'AATT_C
Cuts at: 4111 4489 4111
Size: 378 6424
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3812 3812
Size: 6802
FspI TGC'GCA
Cuts at: 5308 5308
Size: 6802
HaeII r_GCGC'y
Cuts at: 1678 2027 2078 2090 2127 5949 1678
Size: 349 51 12 37 3822 2531
Fragments arranged by size:
3822 2531 349 51 37 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2185 2217 5443 5475 5517
Size: 32 135 32 613 32 3226 32 42
Cuts at: 5517 5549 1373
Size: 32 2626
Fragments arranged by size:
3226 2626 613 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2823 4146 4392 669
Size: 988 281 885 1323 246 3079
Fragments arranged by size:
3079 1323 988 885 281 246
HindIII A'AGCT_T
Cuts at: 748 2205 748
Size: 1457 5345
HpaI GTT'AAC
Cuts at: 4392 4392
Size: 6802
KasI G'GCGC_C
Cuts at: 2123 2123
Size: 6802
KpnI G_GTAC'C
Cuts at: 4127 4127
Size: 6802
MfeI C'AATT_G
Cuts at: 4401 4401
Size: 6802
MluI A'CGCG_T
Cuts at: 6678 6678
Size: 6802
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4011 5916 6100 1305
Size: 2706 1905 184 2007
Fragments arranged by size:
2706 2007 1905 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4980
Fragments arranged by size:
4980 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2841 3028 3557 3746 4760 5119 5278
Size: 648 1675 187 529 189 1014 359 159
Cuts at: 5278 518
Size: 2042
Fragments arranged by size:
2042 1675 1014 648 529 359 189 187
159
MspA1I CmG'CkG
Cuts at: 1606 2014 2032 2065 3779 4218 4895 6043 6288
Size: 408 18 33 1714 439 677 1148 245
Cuts at: 6288 1606
Size: 2120
Fragments arranged by size:
2120 1714 1148 677 439 408 245 33
18
NarI GG'CG_CC
Cuts at: 2124 2124
Size: 6802
NcoI C'CATG_G
Cuts at: 513 1065 3552 513
Size: 552 2487 3763
Fragments arranged by size:
3763 2487 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6802
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6802
NotI GC'GGCC_GC
Cuts at: 4249 4249
Size: 6802
NsiI A_TGCA'T
Cuts at: 2584 2584
Size: 6802
NspI r_CATG'y
Cuts at: 1132 1229 3452 4160 5705 1132
Size: 97 2223 708 1545 2229
Fragments arranged by size:
2229 2223 1545 708 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6802
PciI A'CATG_T
Cuts at: 5701 5701
Size: 6802
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3300 1108
Size: 2192 4610
PfoI T'CCnGG_A
Cuts at: 2808 2808
Size: 6802
PmeI GTTT'AAAC
Cuts at: 4106 4106
Size: 6802
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4871 4903 6384 6416 4871
Size: 32 1481 32 5257
Fragments arranged by size:
5257 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3094 3094
Size: 6802
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6802
PsiI TTA'TAA
Cuts at: 2363 4372 2363
Size: 2009 4793
PstI C_TGCA'G
Cuts at: 830 2031 2058 4154 830
Size: 1201 27 2096 3478
Fragments arranged by size:
3478 2096 1201 27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6802
PvuII CAG'CTG
Cuts at: 2032 3779 2032
Size: 1747 5055
SalI G'TCGA_C
Cuts at: 1936 4144 1936
Size: 2208 4594
SbfI CC_TGCA'GG
Cuts at: 4154 4154
Size: 6802
ScaI AGT'ACT
Cuts at: 3281 5050 6762 3281
Size: 1769 1712 3321
Fragments arranged by size:
3321 1769 1712
SfcI C'TryA_G
Cuts at: 826 1045 2027 2054 4150 5285 5966 6157 826
Size: 219 982 27 2096 1135 681 191 1471
Fragments arranged by size:
2096 1471 1135 982 681 219 191 27
SfoI GGC'GCC
Cuts at: 2125 2125
Size: 6802
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6802
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6802
SmaI CCC'GGG
Cuts at: 1872 4129 1872
Size: 2257 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2202 2386 4906 5807 6069 820
Size: 197 926 259 184 2520 901 262 1553
Fragments arranged by size:
2520 1553 926 901 262 259 197 184
SnaBI TAC'GTA
Cuts at: 493 4593 493
Size: 4100 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6802
SphI G_CATG'C
Cuts at: 1132 4160 1132
Size: 3028 3774
SspI AAT'ATT
Cuts at: 5 52 3115 3879 4726 5
Size: 47 3063 764 847 2081
Fragments arranged by size:
3063 2081 847 764 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6802
StyI C'CwwG_G
Cuts at: 513 1065 2682 3270 3552 4241 513
Size: 552 1617 588 282 689 3074
Fragments arranged by size:
3074 1617 689 588 552 282
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3306 4829 4846 4999 5184 5629 325
Size: 1255 1726 1523 17 153 185 445 1498
Fragments arranged by size:
1726 1523 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2569 3279 5048
Size: 274 80 33 51 1443 592 710 1769
Cuts at: 5048 6760 96
Size: 1712 138
Fragments arranged by size:
1769 1712 1443 710 592 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6762 6794 6762
Size: 32 6770
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6802
XbaI T'CTAG_A
Cuts at: 3388 4138 3388
Size: 750 6052
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6802
XmaI C'CCGG_G
Cuts at: 1870 4127 1870
Size: 2257 4545
XmnI GAAnn'nnTTC
Cuts at: 2435 4931 2435
Size: 2496 4306
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4610 276
Size: 53 83 186 936 3076 2468
Fragments arranged by size:
3076 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BsiWI BsmBI
BstZ17I EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV
NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI