Restriction map for
pFN21AE9532
(Circular) (Six-base) MAPSORT of: pFN21AE9532.seq Check: 2392 from: 1 to: 5815
pFN21AE9532 5815 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3025 3025
Size: 5815
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2487 3625 278
Size: 53 83 186 936 951 1138 2468
Fragments arranged by size:
2468 1138 951 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3158 1937
Size: 1221 4594
Acc65I G'GTAC_C
Cuts at: 3100 3136 3100
Size: 36 5779
AclI AA'CG_TT
Cuts at: 1563 3942 4315 1563
Size: 2379 373 3063
Fragments arranged by size:
3063 2379 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2042 3878 5262 5530 1632
Size: 410 1836 1384 268 1917
Fragments arranged by size:
1917 1836 1384 410 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5618
AflIII A'CryG_T
Cuts at: 1203 2403 4714 5691 1203
Size: 1200 2311 977 1327
Fragments arranged by size:
2311 1327 1200 977
AgeI A'CCGG_T
Cuts at: 4646 4646
Size: 5815
AhdI GACnn_n'nnGTC
Cuts at: 4544 4544
Size: 5815
AleI CACnn'nnGTG
Cuts at: 1166 2165 1166
Size: 999 4816
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5815
AlwNI CAG_nnn'CTG
Cuts at: 3558 5130 3558
Size: 1572 4243
ApaI G_GGCC'C
Cuts at: 2533 2642 2533
Size: 109 5706
ApaLI G'TGCA_C
Cuts at: 3872 5028 3872
Size: 1156 4659
ApoI r'AATT_y
Cuts at: 1493 3124 3350 3502 5611 1493
Size: 1631 226 152 2109 1697
Fragments arranged by size:
2109 1697 1631 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4369 160
Size: 872 3337 1606
Fragments arranged by size:
3337 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5815
AvaI C'yCGr_G
Cuts at: 1870 1943 2147 2749 2757 3140 1870
Size: 73 204 602 8 383 4545
Fragments arranged by size:
4545 602 383 204 73 8
BamHI G'GATC_C
Cuts at: 3145 3512 3145
Size: 367 5448
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2132 3100 3136 4591 618
Size: 325 217 627 345 968 36 1455 1842
Fragments arranged by size:
1842 1455 968 627 345 325 217 36
BanII G_rGCy'C
Cuts at: 1391 2013 2084 2154 2175 2202 2533 2573 2642
Size: 622 71 70 21 27 331 40 69
Cuts at: 2642 2756 3134 1391
Size: 114 378 4072
Fragments arranged by size:
4072 622 378 331 114 71 70 69
40 27 21
BbeI G_GCGC'C
Cuts at: 2136 2136
Size: 5815
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2258 2839 928
Size: 971 359 581 3904
Fragments arranged by size:
3904 971 581 359
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4006 4040 1903
Size: 34 2069 34 3678
Fragments arranged by size:
3678 2069 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3707 4917 3707
Size: 1210 4605
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5815
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2149 2528 4426
Size: 107 122 71 631 605 477 379 1898
Cuts at: 4426 136
Size: 1525
Fragments arranged by size:
1898 1525 631 605 477 379 122 107
71
BglII A'GATC_T
Cuts at: 5810 5810
Size: 5815
BlpI GC'TnA_GC
Cuts at: 3232 3232
Size: 5815
Bme1580I G_kGCm'C
Cuts at: 1790 2330 2479 2533 2642 3876 5032 1790
Size: 540 149 54 109 1234 1156 2573
Fragments arranged by size:
2573 1234 1156 540 149 109 54
BmgBI CAC'GTC
Cuts at: 1318 2493 3031 3070 1318
Size: 1175 538 39 4063
Fragments arranged by size:
4063 1175 538 39
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2644 3546 4504
Size: 416 53 83 399 24 1227 902 958
Cuts at: 4504 442
Size: 1753
Fragments arranged by size:
1753 1227 958 902 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5815
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4475 1239
Size: 3236 2579
Bpu10I CC'TnA_GC
Cuts at: 2078 2078
Size: 5815
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2138 3940 4805 5103 2138
Size: 1802 865 298 2850
Fragments arranged by size:
2850 1802 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4478 882
Size: 617 2979 2219
Fragments arranged by size:
2979 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3606 493
Size: 711 2402 2702
Fragments arranged by size:
2702 2402 711
BsaBI GATnn'nnATC
Cuts at: 5809 5809
Size: 5815
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2133 2484 2834 3622
Size: 53 83 186 936 600 351 350 788
Cuts at: 3622 4004 4639 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 788 635 600 382 351 350
186 83 53
BsaWI w'CCGG_w
Cuts at: 2769 4248 4646 4920 5067 5478 2769
Size: 1479 398 274 147 411 3106
Fragments arranged by size:
3106 1479 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5590
Fragments arranged by size:
5590 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5815
BseYI C'CCAG_C
Cuts at: 2866 5018 5514 2866
Size: 2152 496 3167
Fragments arranged by size:
3167 2152 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2899 3053 1163
Size: 573 1163 154 3925
Fragments arranged by size:
3925 1163 573 154
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3265 4026 5054 1540
Size: 275 186 1264 761 1028 2301
Fragments arranged by size:
2301 1264 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2691 3876 3961 5032 2691
Size: 1185 85 1071 3474
Fragments arranged by size:
3474 1185 1071 85
BsmI GAATG_Cn'
Cuts at: 2368 3326 3419 2368
Size: 958 93 4764
Fragments arranged by size:
4764 958 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3089 3089
Size: 5815
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2084 2154 2175 2202 2330 2479
Size: 399 223 71 70 21 27 128 149
Cuts at: 2479 2533 2573 2642 2691 2756 3134 3876 3961
Size: 54 40 69 49 65 378 742 85
Cuts at: 3961 5032 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 378 223 149 128
85 71 70 69 65 54 49 40
27 21
BspEI T'CCGG_A
Cuts at: 5478 5478
Size: 5815
BspHI T'CATG_A
Cuts at: 2715 3704 5434 2715
Size: 989 1730 3096
Fragments arranged by size:
3096 1730 989
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2784 3025 3170 844
Size: 1940 241 145 3489
Fragments arranged by size:
3489 1940 241 145
BsrBI CCG'CTC
Cuts at: 1394 1823 3702 1394
Size: 429 1879 3507
Fragments arranged by size:
3507 1879 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2382 3057 4310 4484 65
Size: 1183 1134 675 1253 174 1396
Fragments arranged by size:
1396 1253 1183 1134 675 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4459 4646 1700
Size: 110 2649 187 2869
Fragments arranged by size:
2869 2649 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5815
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5815
BssSI C'ACGA_G
Cuts at: 3875 4887 3875
Size: 1012 4803
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2113 3003 794
Size: 1319 890 3606
Fragments arranged by size:
3606 1319 890
BstBI TT'CG_AA
Cuts at: 4619 4619
Size: 5815
BstEII G'GTnAC_C
Cuts at: 1184 2158 1184
Size: 974 4841
BstXI CCAn_nnnn'nTGG
Cuts at: 2440 3070 2440
Size: 630 5185
BstYI r'GATC_y
Cuts at: 1972 2613 3145 3512 3897 3914 5366 5452 5464
Size: 641 532 367 385 17 1452 86 12
Cuts at: 5464 5810 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 641 532 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2234 2625 2234
Size: 391 5424
BtgI C'CryG_G
Cuts at: 513 1065 2004 2128 2731 2895 3104 513
Size: 552 939 124 603 164 209 3224
Fragments arranged by size:
3224 939 603 552 209 164 124
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5076
Fragments arranged by size:
5076 683 56
BtsI GCAGTG_nn'
Cuts at: 3331 4125 4145 3331
Size: 794 20 5001
Fragments arranged by size:
5001 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5815
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5780
DraI TTT'AAA
Cuts at: 3119 3119
Size: 5815
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2300 4822 809
Size: 507 984 2522 1802
Fragments arranged by size:
2522 1802 984 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2181 2289 2351
Size: 54 1425 50 275 18 351 108 62
Cuts at: 2351 2520 2898 3262 4151 5780 8
Size: 169 378 364 889 1629 43
Fragments arranged by size:
1629 1425 889 378 364 351 275 169
108 62 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3262 1537
Size: 275 1450 4090
Fragments arranged by size:
4090 1450 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2021 2586 2672 3745 1353
Size: 668 565 86 1073 3423
Fragments arranged by size:
3423 1073 668 565 86
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2729 4402 4776 4922 387
Size: 1064 138 1140 1673 374 146 1280
Fragments arranged by size:
1673 1280 1140 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2042 3878 4475 5262 5530 1239
Size: 393 410 1836 597 787 268 1524
Fragments arranged by size:
1836 1524 787 597 410 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2530 2638 2530
Size: 108 5707
EcoRI G'AATT_C
Cuts at: 3124 3502 3124
Size: 378 5437
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3007 3007
Size: 5815
FspI TGC'GCA
Cuts at: 3053 4321 3053
Size: 1268 4547
HaeII r_GCGC'y
Cuts at: 1678 2136 4962 1678
Size: 458 2826 2531
Fragments arranged by size:
2826 2531 458
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2428 2460 4456 4488 4530
Size: 32 135 32 856 32 1996 32 42
Cuts at: 4530 4562 1373
Size: 32 2626
Fragments arranged by size:
2626 1996 856 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2935 3159 3405 669
Size: 988 281 997 224 246 3079
Fragments arranged by size:
3079 997 988 281 246 224
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5815
HpaI GTT'AAC
Cuts at: 3405 3405
Size: 5815
KasI G'GCGC_C
Cuts at: 2132 2132
Size: 5815
KpnI G_GTAC'C
Cuts at: 3104 3140 3104
Size: 36 5779
MfeI C'AATT_G
Cuts at: 3414 3414
Size: 5815
MluI A'CGCG_T
Cuts at: 5691 5691
Size: 5815
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2173 4929 5113 1305
Size: 868 2756 184 2007
Fragments arranged by size:
2756 2007 868 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2522 2900 10
Size: 54 1425 343 690 378 2925
Fragments arranged by size:
2925 1425 690 378 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2165 2246 2438 3773 4132 4291 518
Size: 648 999 81 192 1335 359 159 2042
Fragments arranged by size:
2042 1335 999 648 359 192 159 81
MspA1I CmG'CkG
Cuts at: 1606 2940 3106 3231 3908 5056 5301 1606
Size: 1334 166 125 677 1148 245 2120
Fragments arranged by size:
2120 1334 1148 677 245 166 125
NarI GG'CG_CC
Cuts at: 2133 2133
Size: 5815
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4324
Fragments arranged by size:
4324 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5815
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5815
NotI GC'GGCC_GC
Cuts at: 3262 3262
Size: 5815
NspI r_CATG'y
Cuts at: 1132 1229 2407 3173 4718 1132
Size: 97 1178 766 1545 2229
Fragments arranged by size:
2229 1545 1178 766 97
PasI CC'CwG_GG
Cuts at: 1348 2923 1348
Size: 1575 4240
PciI A'CATG_T
Cuts at: 2403 4714 2403
Size: 2311 3504
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2866 1108
Size: 1758 4057
PfoI T'CCnGG_A
Cuts at: 2967 2967
Size: 5815
PmeI GTTT'AAAC
Cuts at: 3119 3119
Size: 5815
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3884 3916 5397 5429 3884
Size: 32 1481 32 4270
Fragments arranged by size:
4270 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2837 1613
Size: 1224 4591
PsiI TTA'TAA
Cuts at: 3385 3385
Size: 5815
PspOMI G'GGCC_C
Cuts at: 2529 2638 2529
Size: 109 5706
PstI C_TGCA'G
Cuts at: 830 3167 830
Size: 2337 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5815
PvuII CAG'CTG
Cuts at: 2940 2940
Size: 5815
SacII CC_GC'GG
Cuts at: 3107 3107
Size: 5815
SalI G'TCGA_C
Cuts at: 1936 3157 1936
Size: 1221 4594
SbfI CC_TGCA'GG
Cuts at: 3167 3167
Size: 5815
ScaI AGT'ACT
Cuts at: 4063 5775 4063
Size: 1712 4103
SfcI C'TryA_G
Cuts at: 826 1045 2707 3163 4298 4979 5170 826
Size: 219 1662 456 1135 681 191 1471
Fragments arranged by size:
1662 1471 1135 681 456 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2528 2528
Size: 5815
SfoI GGC'GCC
Cuts at: 2134 2134
Size: 5815
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5815
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5815
SmaI CCC'GGG
Cuts at: 1872 3142 1872
Size: 1270 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2153 3919 4820 5082 820
Size: 197 926 210 1766 901 262 1553
Fragments arranged by size:
1766 1553 926 901 262 210 197
SnaBI TAC'GTA
Cuts at: 493 3606 493
Size: 3113 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5815
SphI G_CATG'C
Cuts at: 1132 3173 1132
Size: 2041 3774
SspI AAT'ATT
Cuts at: 5 52 2266 3739 5
Size: 47 2214 1473 2081
Fragments arranged by size:
2214 2081 1473 47
StuI AGG'CCT
Cuts at: 1517 2987 1517
Size: 1470 4345
StyI C'CwwG_G
Cuts at: 513 1065 2004 2860 3063 3254 513
Size: 552 939 856 203 191 3074
Fragments arranged by size:
3074 939 856 552 203 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3842 3859 4012 4197 4642 325
Size: 1255 2262 17 153 185 445 1498
Fragments arranged by size:
2262 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4061 5773 96
Size: 274 80 33 51 1443 2084 1712 138
Fragments arranged by size:
2084 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5775 5807 5775
Size: 32 5783
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5815
XbaI T'CTAG_A
Cuts at: 3151 3151
Size: 5815
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2530 2530
Size: 5815
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5815
XmaI C'CCGG_G
Cuts at: 1870 3140 1870
Size: 1270 4545
XmnI GAAnn'nnTTC
Cuts at: 3944 3944
Size: 5815
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2485 3623 276
Size: 53 83 186 936 951 1138 2468
Fragments arranged by size:
2468 1138 951 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacII SalI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI
PsrI RsrII SacI SanDI SapI SexAI SrfI SwaI