Restriction map for
pFN21AE9530
(Circular) (Six-base) MAPSORT of: pFN21AE9530.seq Check: 6976 from: 1 to: 5950
pFN21AE9530 5950 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3088 3088
Size: 5950
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3760 278
Size: 53 83 186 936 2224 2468
Fragments arranged by size:
2468 2224 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3293 1937
Size: 1356 4594
Acc65I G'GTAC_C
Cuts at: 3271 3271
Size: 5950
AclI AA'CG_TT
Cuts at: 1563 4077 4450 1563
Size: 2514 373 3063
Fragments arranged by size:
3063 2514 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2009 2242 2860 4013 5397 5665 1632
Size: 377 233 618 1153 1384 268 1917
Fragments arranged by size:
1917 1384 1153 618 377 268 233
AfeI AGC'GCT
Cuts at: 2435 2435
Size: 5950
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5753
AflIII A'CryG_T
Cuts at: 1203 4849 5826 1203
Size: 3646 977 1327
Fragments arranged by size:
3646 1327 977
AgeI A'CCGG_T
Cuts at: 4781 4781
Size: 5950
AhdI GACnn_n'nnGTC
Cuts at: 4679 4679
Size: 5950
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5950
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5950
AlwNI CAG_nnn'CTG
Cuts at: 3693 5265 3693
Size: 1572 4378
ApaI G_GGCC'C
Cuts at: 2352 2494 2851 2352
Size: 142 357 5451
Fragments arranged by size:
5451 357 142
ApaLI G'TGCA_C
Cuts at: 4007 5163 4007
Size: 1156 4794
ApoI r'AATT_y
Cuts at: 1493 3259 3485 3637 5746 1493
Size: 1766 226 152 2109 1697
Fragments arranged by size:
2109 1766 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4504 160
Size: 872 3472 1606
Fragments arranged by size:
3472 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5950
AvaI C'yCGr_G
Cuts at: 1870 1943 3275 1870
Size: 73 1332 4545
Fragments arranged by size:
4545 1332 73
BamHI G'GATC_C
Cuts at: 2499 3280 3647 2499
Size: 781 367 4802
Fragments arranged by size:
4802 781 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2484 3271 4726 618
Size: 325 217 627 697 787 1455 1842
Fragments arranged by size:
1842 1455 787 697 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2352 2494 2851 2963 3269 1391
Size: 961 142 357 112 306 4072
Fragments arranged by size:
4072 961 357 306 142 112
BbeI G_GCGC'C
Cuts at: 2488 2488
Size: 5950
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2240 2938 928
Size: 971 341 698 3940
Fragments arranged by size:
3940 971 698 341
BbvCI CC'TCA_GC
Cuts at: 2408 2408
Size: 5950
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4141 4175 1903
Size: 34 2204 34 3678
Fragments arranged by size:
3678 2204 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3842 5052 3842
Size: 1210 4740
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5950
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4561 136
Size: 107 122 71 631 605 2889 1525
Fragments arranged by size:
2889 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5945 5945
Size: 5950
BlpI GC'TnA_GC
Cuts at: 2962 3367 2962
Size: 405 5545
Bme1580I G_kGCm'C
Cuts at: 1790 2352 2494 2851 4011 5167 1790
Size: 562 142 357 1160 1156 2573
Fragments arranged by size:
2573 1160 1156 562 357 142
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5950
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3681 4639 442
Size: 416 53 83 399 24 2264 958 1753
Fragments arranged by size:
2264 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5950
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2327 4610 1239
Size: 1088 2283 2579
Fragments arranged by size:
2579 2283 1088
Bpu10I CC'TnA_GC
Cuts at: 2408 2957 2408
Size: 549 5401
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2771 4075 4940 5238 2771
Size: 1304 865 298 3483
Fragments arranged by size:
3483 1304 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2275 2378 4613 882
Size: 617 776 103 2235 2219
Fragments arranged by size:
2235 2219 776 617 103
BsaAI yAC'GTr
Cuts at: 493 1204 2440 3741 493
Size: 711 1236 1301 2702
Fragments arranged by size:
2702 1301 1236 711
BsaBI GATnn'nnATC
Cuts at: 5944 5944
Size: 5950
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2237 2485 3757 4139
Size: 53 83 186 936 704 248 1272 382
Cuts at: 4139 4774 275
Size: 635 1451
Fragments arranged by size:
1451 1272 936 704 635 382 248 186
83 53
BsaWI w'CCGG_w
Cuts at: 4383 4781 5055 5202 5613 4383
Size: 398 274 147 411 4720
Fragments arranged by size:
4720 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5725
Fragments arranged by size:
5725 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2108 2672 2903 1186
Size: 922 564 231 4233
Fragments arranged by size:
4233 922 564 231
BseYI C'CCAG_C
Cuts at: 3057 3084 5153 5649 3057
Size: 27 2069 496 3358
Fragments arranged by size:
3358 2069 496 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5377
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3400 4161 5189 1540
Size: 275 186 1399 761 1028 2301
Fragments arranged by size:
2301 1399 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2480 2532 2963 4011 4096 5167 2480
Size: 52 431 1048 85 1071 3263
Fragments arranged by size:
3263 1071 1048 431 85 52
BsmI GAATG_Cn'
Cuts at: 2035 2304 2773 3014 3113 3461 3554 2035
Size: 269 469 241 99 348 93 4431
Fragments arranged by size:
4431 469 348 269 241 99 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2352 2480 2494 2532 2851 2963 3269
Size: 399 562 128 14 38 319 112 306
Cuts at: 3269 4011 4096 5167 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 562 399 319 306 128
112 85 38 14
BspEI T'CCGG_A
Cuts at: 5613 5613
Size: 5950
BspHI T'CATG_A
Cuts at: 3839 5569 3839
Size: 1730 4220
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3088 3305 844
Size: 2244 217 3489
Fragments arranged by size:
3489 2244 217
BsrBI CCG'CTC
Cuts at: 1394 1823 3837 1394
Size: 429 2014 3507
Fragments arranged by size:
3507 2014 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2197 4445 4619 65
Size: 1183 949 2248 174 1396
Fragments arranged by size:
2248 1396 1183 949 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4594 4781 1700
Size: 110 2784 187 2869
Fragments arranged by size:
2869 2784 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5950
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5950
BssSI C'ACGA_G
Cuts at: 4010 5022 4010
Size: 1012 4938
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5950
BstBI TT'CG_AA
Cuts at: 4754 4754
Size: 5950
BstEII G'GTnAC_C
Cuts at: 1184 2659 1184
Size: 1475 4475
BstYI r'GATC_y
Cuts at: 1972 2040 2062 2499 2637 3280 3647 4032 4049
Size: 68 22 437 138 643 367 385 17
Cuts at: 4049 5501 5587 5599 5945 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 643 437 385 367 346 138
86 68 22 17 12
Bsu36I CC'TnA_GG
Cuts at: 2495 2495
Size: 5950
BtgI C'CryG_G
Cuts at: 513 1065 2352 2557 513
Size: 552 1287 205 3906
Fragments arranged by size:
3906 1287 552 205
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5211
Fragments arranged by size:
5211 683 56
BtsI GCAGTG_nn'
Cuts at: 3466 4260 4280 3466
Size: 794 20 5136
Fragments arranged by size:
5136 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5950
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2154 2189 529
Size: 35 1590 35 4290
Fragments arranged by size:
4290 1590 35 35
DraI TTT'AAA
Cuts at: 3254 3254
Size: 5950
DraIII CAC_nnn'GTG
Cuts at: 3118 3118
Size: 5950
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2282 4957 809
Size: 507 966 2675 1802
Fragments arranged by size:
2675 1802 966 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2442 2607 3397
Size: 54 1425 50 275 18 612 165 790
Cuts at: 3397 4286 5915 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 790 612 275 165 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3397 1537
Size: 275 1585 4090
Fragments arranged by size:
4090 1585 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2261 2537 2876 3037 3880 1353
Size: 908 276 339 161 843 3423
Fragments arranged by size:
3423 908 843 339 276 161
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4537 4911 5057 387
Size: 1064 138 2948 374 146 1280
Fragments arranged by size:
2948 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2961 2961
Size: 5950
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2009 2242 2327 2860 4013 4610 5397
Size: 393 377 233 85 533 1153 597 787
Cuts at: 5397 5665 1239
Size: 268 1524
Fragments arranged by size:
1524 1153 787 597 533 393 377 268
233 85
EcoO109I rG'GnC_Cy
Cuts at: 2491 2589 2848 3140 2491
Size: 98 259 292 5301
Fragments arranged by size:
5301 292 259 98
EcoRI G'AATT_C
Cuts at: 3259 3637 3259
Size: 378 5572
FspI TGC'GCA
Cuts at: 4456 4456
Size: 5950
HaeII r_GCGC'y
Cuts at: 1678 2437 2488 5097 1678
Size: 759 51 2609 2531
Fragments arranged by size:
2609 2531 759 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4591 4623 4665 4697 1373
Size: 32 135 32 3019 32 42 32 2626
Fragments arranged by size:
3019 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2803 2971 3294 3540 669
Size: 988 281 865 168 323 246 3079
Fragments arranged by size:
3079 988 865 323 281 246 168
HindIII A'AGCT_T
Cuts at: 748 3229 748
Size: 2481 3469
HpaI GTT'AAC
Cuts at: 3540 3540
Size: 5950
KasI G'GCGC_C
Cuts at: 2484 2484
Size: 5950
KpnI G_GTAC'C
Cuts at: 3275 3275
Size: 5950
MfeI C'AATT_G
Cuts at: 3549 3549
Size: 5950
MluI A'CGCG_T
Cuts at: 5826 5826
Size: 5950
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5064 5248 1305
Size: 3759 184 2007
Fragments arranged by size:
3759 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2444 10
Size: 54 1425 343 612 3516
Fragments arranged by size:
3516 1425 612 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3908 4267 4426 518
Size: 648 2742 359 159 2042
Fragments arranged by size:
2742 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2966 3088 3366 4043 5191 5436 1606
Size: 1360 122 278 677 1148 245 2120
Fragments arranged by size:
2120 1360 1148 677 278 245 122
NarI GG'CG_CC
Cuts at: 2485 2485
Size: 5950
NcoI C'CATG_G
Cuts at: 513 1065 2557 513
Size: 552 1492 3906
Fragments arranged by size:
3906 1492 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5950
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5950
NotI GC'GGCC_GC
Cuts at: 3397 3397
Size: 5950
NspI r_CATG'y
Cuts at: 1132 1229 3308 4853 1132
Size: 97 2079 1545 2229
Fragments arranged by size:
2229 2079 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5950
PciI A'CATG_T
Cuts at: 4849 4849
Size: 5950
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3118 1108
Size: 2010 3940
PfoI T'CCnGG_A
Cuts at: 2036 2036
Size: 5950
PmeI GTTT'AAAC
Cuts at: 3254 3254
Size: 5950
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4019 4051 5532 5564 4019
Size: 32 1481 32 4405
Fragments arranged by size:
4405 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2589 3140 2589
Size: 551 5399
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5950
PsiI TTA'TAA
Cuts at: 3520 3520
Size: 5950
PspOMI G'GGCC_C
Cuts at: 2348 2490 2847 2348
Size: 142 357 5451
Fragments arranged by size:
5451 357 142
PstI C_TGCA'G
Cuts at: 830 2220 2475 2792 3302 830
Size: 1390 255 317 510 3478
Fragments arranged by size:
3478 1390 510 317 255
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5950
PvuII CAG'CTG
Cuts at: 2966 2966
Size: 5950
SacI G_AGCT'C
Cuts at: 2963 2963
Size: 5950
SalI G'TCGA_C
Cuts at: 1936 3292 1936
Size: 1356 4594
SanDI GG'GwC_CC
Cuts at: 2589 2589
Size: 5950
SapI GCTCTTCn'nnn_
Cuts at: 2537 2537
Size: 5950
SbfI CC_TGCA'GG
Cuts at: 3302 3302
Size: 5950
ScaI AGT'ACT
Cuts at: 4198 5910 4198
Size: 1712 4238
SfcI C'TryA_G
Cuts at: 826 1045 2216 2471 2788 3298 4433 5114 5305
Size: 219 1171 255 317 510 1135 681 191
Cuts at: 5305 826
Size: 1471
Fragments arranged by size:
1471 1171 1135 681 510 317 255 219
191
SfoI GGC'GCC
Cuts at: 2486 2486
Size: 5950
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5950
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5950
SmaI CCC'GGG
Cuts at: 1872 3277 1872
Size: 1405 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2750 4054 4955 5217 820
Size: 197 926 807 1304 901 262 1553
Fragments arranged by size:
1553 1304 926 901 807 262 197
SnaBI TAC'GTA
Cuts at: 493 3741 493
Size: 3248 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5950
SphI G_CATG'C
Cuts at: 1132 3308 1132
Size: 2176 3774
SspI AAT'ATT
Cuts at: 5 52 2776 3874 5
Size: 47 2724 1098 2081
Fragments arranged by size:
2724 2081 1098 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5950
StyI C'CwwG_G
Cuts at: 513 1065 2293 2557 3389 513
Size: 552 1228 264 832 3074
Fragments arranged by size:
3074 1228 832 552 264
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2880 3143 3977 3994 4147 4332 4777
Size: 1255 1300 263 834 17 153 185 445
Cuts at: 4777 325
Size: 1498
Fragments arranged by size:
1498 1300 1255 834 445 263 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4196 5908 96
Size: 274 80 33 51 1443 2219 1712 138
Fragments arranged by size:
2219 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5910 5942 5910
Size: 32 5918
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5950
XbaI T'CTAG_A
Cuts at: 3286 3286
Size: 5950
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2425 2590 2011
Size: 414 165 5371
Fragments arranged by size:
5371 414 165
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5950
XmaI C'CCGG_G
Cuts at: 1870 3275 1870
Size: 1405 4545
XmnI GAAnn'nnTTC
Cuts at: 2022 3095 4079 2022
Size: 1073 984 3893
Fragments arranged by size:
3893 1073 984
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3758 276
Size: 53 83 186 936 2224 2468
Fragments arranged by size:
2468 2224 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstXI BstZ17I EcoNI EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII
SacII SexAI SfiI SrfI SwaI