Restriction map for
pFN21AE9529
(Circular) (Six-base) MAPSORT of: pFN21AE9529.seq Check: 5353 from: 1 to: 5764
pFN21AE9529 5764 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2483 2483
Size: 5764
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3574 278
Size: 53 83 186 936 2038 2468
Fragments arranged by size:
2468 2038 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3107 1937
Size: 1170 4594
Acc65I G'GTAC_C
Cuts at: 3085 3085
Size: 5764
AclI AA'CG_TT
Cuts at: 1563 3891 4264 1563
Size: 2328 373 3063
Fragments arranged by size:
3063 2328 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2944 3827 5211 5479 1632
Size: 1312 883 1384 268 1917
Fragments arranged by size:
1917 1384 1312 883 268
AfeI AGC'GCT
Cuts at: 2754 2754
Size: 5764
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5567
AflIII A'CryG_T
Cuts at: 1203 4663 5640 1203
Size: 3460 977 1327
Fragments arranged by size:
3460 1327 977
AgeI A'CCGG_T
Cuts at: 4595 4595
Size: 5764
AhdI GACnn_n'nnGTC
Cuts at: 4493 4493
Size: 5764
AleI CACnn'nnGTG
Cuts at: 1166 2477 2768 1166
Size: 1311 291 4162
Fragments arranged by size:
4162 1311 291
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5764
AlwNI CAG_nnn'CTG
Cuts at: 2249 3507 5079 2249
Size: 1258 1572 2934
Fragments arranged by size:
2934 1572 1258
ApaI G_GGCC'C
Cuts at: 2797 2797
Size: 5764
ApaLI G'TGCA_C
Cuts at: 2423 3821 4977 2423
Size: 1398 1156 3210
Fragments arranged by size:
3210 1398 1156
ApoI r'AATT_y
Cuts at: 1493 2822 3073 3299 3451 5560 1493
Size: 1329 251 226 152 2109 1697
Fragments arranged by size:
2109 1697 1329 251 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4318 160
Size: 872 3286 1606
Fragments arranged by size:
3286 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5764
AvaI C'yCGr_G
Cuts at: 1870 1943 2466 3089 1870
Size: 73 523 623 4545
Fragments arranged by size:
4545 623 523 73
AvrII C'CTAG_G
Cuts at: 2161 2161
Size: 5764
BamHI G'GATC_C
Cuts at: 3094 3461 3094
Size: 367 5397
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3085 4540 618
Size: 325 217 627 1298 1455 1842
Fragments arranged by size:
1842 1455 1298 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2034 2326 2797 3083 1391
Size: 643 292 471 286 4072
Fragments arranged by size:
4072 643 471 292 286
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2179 2653 928
Size: 971 280 474 4039
Fragments arranged by size:
4039 971 474 280
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3955 3989 1903
Size: 34 2018 34 3678
Fragments arranged by size:
3678 2018 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3656 4866 3656
Size: 1210 4554
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5764
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2203 2240 4375
Size: 107 122 71 631 605 531 37 2135
Cuts at: 4375 136
Size: 1525
Fragments arranged by size:
2135 1525 631 605 531 122 107 71
37
BglII A'GATC_T
Cuts at: 2551 5759 2551
Size: 3208 2556
BlpI GC'TnA_GC
Cuts at: 3181 3181
Size: 5764
Bme1580I G_kGCm'C
Cuts at: 1790 2427 2797 3825 4981 1790
Size: 637 370 1028 1156 2573
Fragments arranged by size:
2573 1156 1028 637 370
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5764
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3495 4453 442
Size: 416 53 83 399 24 2078 958 1753
Fragments arranged by size:
2078 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5764
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2345 2345
Size: 5764
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2076 4424 1239
Size: 837 2348 2579
Fragments arranged by size:
2579 2348 837
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2527 3889 4754 5052 2527
Size: 1362 865 298 3239
Fragments arranged by size:
3239 1362 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4427 882
Size: 617 2928 2219
Fragments arranged by size:
2928 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3555 493
Size: 711 2351 2702
Fragments arranged by size:
2702 2351 711
BsaBI GATnn'nnATC
Cuts at: 5758 5758
Size: 5764
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3571 3953 4588 275
Size: 53 83 186 936 2038 382 635 1451
Fragments arranged by size:
2038 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4197 4595 4869 5016 5427 4197
Size: 398 274 147 411 4534
Fragments arranged by size:
4534 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5539
Fragments arranged by size:
5539 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2318 2570 2717 1186
Size: 1132 252 147 4233
Fragments arranged by size:
4233 1132 252 147
BseYI C'CCAG_C
Cuts at: 2790 4967 5463 2790
Size: 2177 496 3091
Fragments arranged by size:
3091 2177 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2096 1163
Size: 573 360 4831
Fragments arranged by size:
4831 573 360
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2658 3214 3975 5003 1540
Size: 275 186 657 556 761 1028 2301
Fragments arranged by size:
2301 1028 761 657 556 275 186
BsiHKAI G_wGCw'C
Cuts at: 2034 2427 2484 3825 3910 4981 2034
Size: 393 57 1341 85 1071 2817
Fragments arranged by size:
2817 1341 1071 393 85 57
BsmI GAATG_Cn'
Cuts at: 3275 3368 3275
Size: 93 5671
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2326 2427 2484 2797 3083 3825
Size: 399 244 292 101 57 313 286 742
Cuts at: 3825 3910 4981 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 313 292 286 244
101 85 57
BspEI T'CCGG_A
Cuts at: 5427 5427
Size: 5764
BspHI T'CATG_A
Cuts at: 3653 5383 3653
Size: 1730 4034
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2115 2483 3119 844
Size: 1271 368 636 3489
Fragments arranged by size:
3489 1271 636 368
BsrBI CCG'CTC
Cuts at: 1394 1823 3651 1394
Size: 429 1828 3507
Fragments arranged by size:
3507 1828 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4259 4433 65
Size: 1183 3011 174 1396
Fragments arranged by size:
3011 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2952 4408 4595 1700
Size: 110 1142 1456 187 2869
Fragments arranged by size:
2869 1456 1142 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5764
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5764
BssSI C'ACGA_G
Cuts at: 2768 3824 4836 2768
Size: 1056 1012 3696
Fragments arranged by size:
3696 1056 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2119 794
Size: 1325 4439
BstBI TT'CG_AA
Cuts at: 4568 4568
Size: 5764
BstEII G'GTnAC_C
Cuts at: 1184 2470 1184
Size: 1286 4478
BstXI CCAn_nnnn'nTGG
Cuts at: 2100 2100
Size: 5764
BstYI r'GATC_y
Cuts at: 1972 2551 3094 3461 3846 3863 5315 5401 5413
Size: 579 543 367 385 17 1452 86 12
Cuts at: 5413 5759 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 579 543 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4273
Fragments arranged by size:
4273 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5025
Fragments arranged by size:
5025 683 56
BtsI GCAGTG_nn'
Cuts at: 3280 4074 4094 3280
Size: 794 20 4950
Fragments arranged by size:
4950 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5764
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2595 2630 529
Size: 35 2031 35 3663
Fragments arranged by size:
3663 2031 35 35
DraI TTT'AAA
Cuts at: 3068 3068
Size: 5764
DraIII CAC_nnn'GTG
Cuts at: 2119 2119
Size: 5764
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4771 809
Size: 507 3455 1802
Fragments arranged by size:
3455 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2232 2391 2433
Size: 54 1425 50 275 18 402 159 42
Cuts at: 2433 2892 3211 4100 5729 8
Size: 459 319 889 1629 43
Fragments arranged by size:
1629 1425 889 459 402 319 275 159
54 50 43 42 18
EagI C'GGCC_G
Cuts at: 1537 1812 3211 1537
Size: 275 1399 4090
Fragments arranged by size:
4090 1399 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2063 2963 3694 1353
Size: 710 900 731 3423
Fragments arranged by size:
3423 900 731 710
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2470 3073 4351 4725 4871 387
Size: 1064 138 881 603 1278 374 146 1280
Fragments arranged by size:
1280 1278 1064 881 603 374 146 138
EcoICRI GAG'CTC
Cuts at: 2032 2032
Size: 5764
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2076 2944 3827 4424 5211 5479 1239
Size: 393 444 868 883 597 787 268 1524
Fragments arranged by size:
1524 883 868 787 597 444 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2104 2500 2794 2104
Size: 396 294 5074
Fragments arranged by size:
5074 396 294
EcoRI G'AATT_C
Cuts at: 2822 3073 3451 2822
Size: 251 378 5135
Fragments arranged by size:
5135 378 251
FspI TGC'GCA
Cuts at: 4270 4270
Size: 5764
HaeII r_GCGC'y
Cuts at: 1678 2756 4911 1678
Size: 1078 2155 2531
Fragments arranged by size:
2531 2155 1078
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2021 2053 4405 4437 4479
Size: 32 135 32 449 32 2352 32 42
Cuts at: 4479 4511 1373
Size: 32 2626
Fragments arranged by size:
2626 2352 449 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3108 3354 669
Size: 988 281 1170 246 3079
Fragments arranged by size:
3079 1170 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5764
HpaI GTT'AAC
Cuts at: 3354 3354
Size: 5764
KpnI G_GTAC'C
Cuts at: 3089 3089
Size: 5764
MfeI C'AATT_G
Cuts at: 2888 3363 2888
Size: 475 5289
MluI A'CGCG_T
Cuts at: 5640 5640
Size: 5764
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2067 3070 4878 5062 1305
Size: 762 1003 1808 184 2007
Fragments arranged by size:
2007 1808 1003 762 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2234 2393 2435 2894 10
Size: 54 1425 343 402 159 42 459 2880
Fragments arranged by size:
2880 1425 459 402 343 159 54 42
MslI CAynn'nnrTG
Cuts at: 518 1166 2477 2768 3722 4081 4240 518
Size: 648 1311 291 954 359 159 2042
Fragments arranged by size:
2042 1311 954 648 359 291 159
MspA1I CmG'CkG
Cuts at: 1606 2452 3180 3857 5005 5250 1606
Size: 846 728 677 1148 245 2120
Fragments arranged by size:
2120 1148 846 728 677 245
NaeI GCC'GGC
Cuts at: 2954 2954
Size: 5764
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4273
Fragments arranged by size:
4273 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5764
NgoMIV G'CCGG_C
Cuts at: 2952 2952
Size: 5764
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5764
NotI GC'GGCC_GC
Cuts at: 3211 3211
Size: 5764
NspI r_CATG'y
Cuts at: 1132 1229 3122 4667 1132
Size: 97 1893 1545 2229
Fragments arranged by size:
2229 1893 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5764
PciI A'CATG_T
Cuts at: 4663 4663
Size: 5764
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2680 2973 1108
Size: 1572 293 3899
Fragments arranged by size:
3899 1572 293
PfoI T'CCnGG_A
Cuts at: 2817 2817
Size: 5764
PmeI GTTT'AAAC
Cuts at: 3068 3068
Size: 5764
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3833 3865 5346 5378 3833
Size: 32 1481 32 4219
Fragments arranged by size:
4219 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2104 2104
Size: 5764
PshAI GACnn'nnGTC
Cuts at: 1613 2969 1613
Size: 1356 4408
PsiI TTA'TAA
Cuts at: 2985 3334 2985
Size: 349 5415
PspOMI G'GGCC_C
Cuts at: 2793 2793
Size: 5764
PstI C_TGCA'G
Cuts at: 830 3116 830
Size: 2286 3478
PvuI CG_AT'CG
Cuts at: 2001 2658 2001
Size: 657 5107
SacI G_AGCT'C
Cuts at: 2034 2034
Size: 5764
SalI G'TCGA_C
Cuts at: 1936 3106 1936
Size: 1170 4594
SapI GCTCTTCn'nnn_
Cuts at: 2063 2963 2063
Size: 900 4864
SbfI CC_TGCA'GG
Cuts at: 3116 3116
Size: 5764
ScaI AGT'ACT
Cuts at: 4012 5724 4012
Size: 1712 4052
SfcI C'TryA_G
Cuts at: 826 1045 3112 4247 4928 5119 826
Size: 219 2067 1135 681 191 1471
Fragments arranged by size:
2067 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5764
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5764
SmaI CCC'GGG
Cuts at: 1872 2468 3091 1872
Size: 596 623 4545
Fragments arranged by size:
4545 623 596
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2506 3868 4769 5031 820
Size: 197 926 563 1362 901 262 1553
Fragments arranged by size:
1553 1362 926 901 563 262 197
SnaBI TAC'GTA
Cuts at: 493 3555 493
Size: 3062 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5764
SphI G_CATG'C
Cuts at: 1132 3122 1132
Size: 1990 3774
SspI AAT'ATT
Cuts at: 5 52 2626 3688 5
Size: 47 2574 1062 2081
Fragments arranged by size:
2574 2081 1062 47
StuI AGG'CCT
Cuts at: 1517 2594 1517
Size: 1077 4687
StyI C'CwwG_G
Cuts at: 513 1065 2004 2161 2967 3203 513
Size: 552 939 157 806 236 3074
Fragments arranged by size:
3074 939 806 552 236 157
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3791 3808 3961 4146 4591 325
Size: 1255 2211 17 153 185 445 1498
Fragments arranged by size:
2211 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4010 5722 96
Size: 274 80 33 51 1443 2033 1712 138
Fragments arranged by size:
2033 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5724 5756 5724
Size: 32 5732
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5764
XbaI T'CTAG_A
Cuts at: 2026 3100 2026
Size: 1074 4690
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5764
XmaI C'CCGG_G
Cuts at: 1870 2466 3089 1870
Size: 596 623 4545
Fragments arranged by size:
4545 623 596
XmnI GAAnn'nnTTC
Cuts at: 3893 3893
Size: 5764
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3572 276
Size: 53 83 186 936 2038 2468
Fragments arranged by size:
2468 2038 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI
BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PmlI PspXI PsrI
PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI