Restriction map for
pFN21AE9528
(Circular) (Six-base) MAPSORT of: pFN21AE9528.seq Check: 7485 from: 1 to: 5857
pFN21AE9528 5857 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3667 278
Size: 53 83 186 936 2131 2468
Fragments arranged by size:
2468 2131 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2301 3200 1937
Size: 364 899 4594
Fragments arranged by size:
4594 899 364
Acc65I G'GTAC_C
Cuts at: 3178 3178
Size: 5857
AclI AA'CG_TT
Cuts at: 1563 3984 4357 1563
Size: 2421 373 3063
Fragments arranged by size:
3063 2421 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3920 5304 5572 1632
Size: 2288 1384 268 1917
Fragments arranged by size:
2288 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5660
AflIII A'CryG_T
Cuts at: 1203 2592 4756 5733 1203
Size: 1389 2164 977 1327
Fragments arranged by size:
2164 1389 1327 977
AgeI A'CCGG_T
Cuts at: 4688 4688
Size: 5857
AhdI GACnn_n'nnGTC
Cuts at: 4586 4586
Size: 5857
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5857
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5857
AlwNI CAG_nnn'CTG
Cuts at: 2080 3600 5172 2080
Size: 1520 1572 2765
Fragments arranged by size:
2765 1572 1520
ApaLI G'TGCA_C
Cuts at: 3914 5070 3914
Size: 1156 4701
ApoI r'AATT_y
Cuts at: 1493 2008 3166 3392 3544 5653 1493
Size: 515 1158 226 152 2109 1697
Fragments arranged by size:
2109 1697 1158 515 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2309 4411 160
Size: 872 1277 2102 1606
Fragments arranged by size:
2102 1606 1277 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5857
AvaI C'yCGr_G
Cuts at: 1870 1943 2439 3030 3182 1870
Size: 73 496 591 152 4545
Fragments arranged by size:
4545 591 496 152 73
BamHI G'GATC_C
Cuts at: 3187 3554 3187
Size: 367 5490
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3178 4633 618
Size: 325 217 627 1391 1455 1842
Fragments arranged by size:
1842 1455 1391 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3176 1391
Size: 1785 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4886
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4048 4082 1903
Size: 34 2111 34 3678
Fragments arranged by size:
3678 2111 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3749 4959 3749
Size: 1210 4647
BclI T'GATC_A
Cuts at: 1548 2154 2187 2602 1548
Size: 606 33 415 4803
Fragments arranged by size:
4803 606 415 33
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4468 136
Size: 107 122 71 631 605 2796 1525
Fragments arranged by size:
2796 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5852 5852
Size: 5857
BlpI GC'TnA_GC
Cuts at: 3274 3274
Size: 5857
Bme1580I G_kGCm'C
Cuts at: 1790 3918 5074 1790
Size: 2128 1156 2573
Fragments arranged by size:
2573 2128 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5857
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2329 3104 3588
Size: 416 53 83 399 24 912 775 484
Cuts at: 3588 4546 442
Size: 958 1753
Fragments arranged by size:
1753 958 912 775 484 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5857
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4517 1239
Size: 3278 2579
Bpu10I CC'TnA_GC
Cuts at: 2131 2131
Size: 5857
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3982 4847 5145 3982
Size: 865 298 4694
Fragments arranged by size:
4694 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2443 3033 4520 882
Size: 617 944 590 1487 2219
Fragments arranged by size:
2219 1487 944 617 590
BsaAI yAC'GTr
Cuts at: 493 1204 2593 3648 493
Size: 711 1389 1055 2702
Fragments arranged by size:
2702 1389 1055 711
BsaBI GATnn'nnATC
Cuts at: 2103 2192 2868 5851 2103
Size: 89 676 2983 2109
Fragments arranged by size:
2983 2109 676 89
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3664 4046 4681 275
Size: 53 83 186 936 2131 382 635 1451
Fragments arranged by size:
2131 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3024 4290 4688 4962 5109 5520 3024
Size: 1266 398 274 147 411 3361
Fragments arranged by size:
3361 1266 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5632
Fragments arranged by size:
5632 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2636 1186
Size: 1450 4407
BseYI C'CCAG_C
Cuts at: 2073 5060 5556 2073
Size: 2987 496 2374
Fragments arranged by size:
2987 2374 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5284
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3307 4068 5096 1540
Size: 275 186 1306 761 1028 2301
Fragments arranged by size:
2301 1306 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3918 4003 5074 3918
Size: 85 1071 4701
Fragments arranged by size:
4701 1071 85
BsmI GAATG_Cn'
Cuts at: 2074 3368 3461 2074
Size: 1294 93 4470
Fragments arranged by size:
4470 1294 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3176 3918 4003 5074 1391
Size: 399 1386 742 85 1071 2174
Fragments arranged by size:
2174 1386 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5520 5520
Size: 5857
BspHI T'CATG_A
Cuts at: 3746 5476 3746
Size: 1730 4127
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2897 3212 844
Size: 2053 315 3489
Fragments arranged by size:
3489 2053 315
BsrBI CCG'CTC
Cuts at: 1394 1823 3744 1394
Size: 429 1921 3507
Fragments arranged by size:
3507 1921 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2369 2403 2880 2937 4352 4526 65
Size: 1183 1121 34 477 57 1415 174 1396
Fragments arranged by size:
1415 1396 1183 1121 477 174 57 34
BsrFI r'CCGG_y
Cuts at: 1700 1810 4501 4688 1700
Size: 110 2691 187 2869
Fragments arranged by size:
2869 2691 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5857
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5857
BssSI C'ACGA_G
Cuts at: 3917 4929 3917
Size: 1012 4845
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5857
BstBI TT'CG_AA
Cuts at: 4661 4661
Size: 5857
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5857
BstYI r'GATC_y
Cuts at: 1972 3187 3554 3939 3956 5408 5494 5506 5852
Size: 1215 367 385 17 1452 86 12 346
Cuts at: 5852 1972
Size: 1977
Fragments arranged by size:
1977 1452 1215 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5305
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2351 487
Size: 683 56 1125 3993
Fragments arranged by size:
3993 1125 683 56
BtsI GCAGTG_nn'
Cuts at: 3373 4167 4187 3373
Size: 794 20 5043
Fragments arranged by size:
5043 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5857
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5822
DraI TTT'AAA
Cuts at: 3121 3161 3121
Size: 40 5817
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2321 4864 809
Size: 507 1005 2543 1802
Fragments arranged by size:
2543 1802 1005 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2115 3304 4193
Size: 54 1425 50 275 18 285 1189 889
Cuts at: 4193 5822 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1189 889 285 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3304 1537
Size: 275 1492 4090
Fragments arranged by size:
4090 1492 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3787 1353
Size: 2434 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4444 4818 4964 387
Size: 1064 138 2855 374 146 1280
Fragments arranged by size:
2855 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3920 4517 5304 5572 1239
Size: 393 2288 597 787 268 1524
Fragments arranged by size:
2288 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2494 2494
Size: 5857
EcoRI G'AATT_C
Cuts at: 2008 3166 3544 2008
Size: 1158 378 4321
Fragments arranged by size:
4321 1158 378
EcoRV GAT'ATC
Cuts at: 2937 2937
Size: 5857
FspI TGC'GCA
Cuts at: 4363 4363
Size: 5857
HaeII r_GCGC'y
Cuts at: 1678 5004 1678
Size: 3326 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2139 2171 3011 3043 4498
Size: 32 135 32 567 32 840 32 1455
Cuts at: 4498 4530 4572 4604 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1455 840 567 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2950 3201 3447 669
Size: 988 281 1012 251 246 3079
Fragments arranged by size:
3079 1012 988 281 251 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5857
HpaI GTT'AAC
Cuts at: 3447 3447
Size: 5857
KpnI G_GTAC'C
Cuts at: 3182 3182
Size: 5857
MfeI C'AATT_G
Cuts at: 3456 3456
Size: 5857
MluI A'CGCG_T
Cuts at: 5733 5733
Size: 5857
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4971 5155 1305
Size: 3666 184 2007
Fragments arranged by size:
3666 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2117 10
Size: 54 1425 343 285 3750
Fragments arranged by size:
3750 1425 343 285 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2309 2408 2469 2930 3075 3815 4174
Size: 648 1143 99 61 461 145 740 359
Cuts at: 4174 4333 518
Size: 159 2042
Fragments arranged by size:
2042 1143 740 648 461 359 159 145
99 61
MspA1I CmG'CkG
Cuts at: 1606 2425 3273 3950 5098 5343 1606
Size: 819 848 677 1148 245 2120
Fragments arranged by size:
2120 1148 848 819 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5305
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5857
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5857
NotI GC'GGCC_GC
Cuts at: 3304 3304
Size: 5857
NspI r_CATG'y
Cuts at: 1132 1229 2468 3215 4760 1132
Size: 97 1239 747 1545 2229
Fragments arranged by size:
2229 1545 1239 747 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5857
PciI A'CATG_T
Cuts at: 4756 4756
Size: 5857
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2080 1108
Size: 972 4885
PmeI GTTT'AAAC
Cuts at: 3161 3161
Size: 5857
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3926 3958 5439 5471 3926
Size: 32 1481 32 4312
Fragments arranged by size:
4312 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2494 2494
Size: 5857
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5857
PsiI TTA'TAA
Cuts at: 3427 3427
Size: 5857
PspXI vC'TCGA_Gb
Cuts at: 2439 2439
Size: 5857
PstI C_TGCA'G
Cuts at: 830 2661 3209 830
Size: 1831 548 3478
Fragments arranged by size:
3478 1831 548
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5857
PvuII CAG'CTG
Cuts at: 2425 2425
Size: 5857
SalI G'TCGA_C
Cuts at: 1936 3199 1936
Size: 1263 4594
SbfI CC_TGCA'GG
Cuts at: 3209 3209
Size: 5857
ScaI AGT'ACT
Cuts at: 4105 5817 4105
Size: 1712 4145
SfcI C'TryA_G
Cuts at: 826 1045 2657 3205 4340 5021 5212 826
Size: 219 1612 548 1135 681 191 1471
Fragments arranged by size:
1612 1471 1135 681 548 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5857
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5857
SmaI CCC'GGG
Cuts at: 1872 3184 1872
Size: 1312 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2439 3961 4862 5124 820
Size: 197 926 496 1522 901 262 1553
Fragments arranged by size:
1553 1522 926 901 496 262 197
SnaBI TAC'GTA
Cuts at: 493 3648 493
Size: 3155 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5857
SphI G_CATG'C
Cuts at: 1132 3215 1132
Size: 2083 3774
SspI AAT'ATT
Cuts at: 5 52 3781 5
Size: 47 3729 2081
Fragments arranged by size:
3729 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5857
StyI C'CwwG_G
Cuts at: 513 1065 2112 3296 513
Size: 552 1047 1184 3074
Fragments arranged by size:
3074 1184 1047 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3884 3901 4054 4239 4684 325
Size: 1255 2304 17 153 185 445 1498
Fragments arranged by size:
2304 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2760 4103 5815
Size: 274 80 33 51 1443 783 1343 1712
Cuts at: 5815 96
Size: 138
Fragments arranged by size:
1712 1443 1343 783 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5817 5849 5817
Size: 32 5825
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5857
XbaI T'CTAG_A
Cuts at: 3193 3193
Size: 5857
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2324 2392 2647 2324
Size: 68 255 5534
Fragments arranged by size:
5534 255 68
XhoI C'TCGA_G
Cuts at: 1943 2439 1943
Size: 496 5361
XmaI C'CCGG_G
Cuts at: 1870 3182 1870
Size: 1312 4545
XmnI GAAnn'nnTTC
Cuts at: 3986 3986
Size: 5857
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3665 276
Size: 53 83 186 936 2131 2468
Fragments arranged by size:
2468 2131 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI