Restriction map for
pFN21AE9526
(Circular) (Six-base) MAPSORT of: pFN21AE9526.seq Check: 6762 from: 1 to: 6019
pFN21AE9526 6019 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3829 278
Size: 53 83 186 936 2293 2468
Fragments arranged by size:
2468 2293 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3362 1937
Size: 1425 4594
Acc65I G'GTAC_C
Cuts at: 3340 3340
Size: 6019
AclI AA'CG_TT
Cuts at: 1563 4146 4519 1563
Size: 2583 373 3063
Fragments arranged by size:
3063 2583 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2224 2440 3182 4082 5466 5734 1632
Size: 592 216 742 900 1384 268 1917
Fragments arranged by size:
1917 1384 900 742 592 268 216
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5822
AflIII A'CryG_T
Cuts at: 1203 4918 5895 1203
Size: 3715 977 1327
Fragments arranged by size:
3715 1327 977
AgeI A'CCGG_T
Cuts at: 2810 4850 2810
Size: 2040 3979
AhdI GACnn_n'nnGTC
Cuts at: 2296 4748 2296
Size: 2452 3567
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6019
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6019
AlwNI CAG_nnn'CTG
Cuts at: 3076 3109 3219 3762 5334 3076
Size: 33 110 543 1572 3761
Fragments arranged by size:
3761 1572 543 110 33
ApaLI G'TGCA_C
Cuts at: 4076 5232 4076
Size: 1156 4863
ApoI r'AATT_y
Cuts at: 1493 2916 3328 3554 3706 5815 1493
Size: 1423 412 226 152 2109 1697
Fragments arranged by size:
2109 1697 1423 412 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4573 160
Size: 872 3541 1606
Fragments arranged by size:
3541 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6019
AvaI C'yCGr_G
Cuts at: 1870 1943 3092 3344 1870
Size: 73 1149 252 4545
Fragments arranged by size:
4545 1149 252 73
BamHI G'GATC_C
Cuts at: 3349 3716 3349
Size: 367 5652
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2221 3183 3254 3340 4795
Size: 325 217 627 434 962 71 86 1455
Cuts at: 4795 618
Size: 1842
Fragments arranged by size:
1842 1455 962 627 434 325 217 86
71
BanII G_rGCy'C
Cuts at: 1391 2679 3338 1391
Size: 1288 659 4072
Fragments arranged by size:
4072 1288 659
BbeI G_GCGC'C
Cuts at: 3258 3258
Size: 6019
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2230 2617 2811 928
Size: 971 331 387 194 4136
Fragments arranged by size:
4136 971 387 331 194
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3165 3199 4210 4244 1903
Size: 34 50 34 1144 34 1011 34 3678
Fragments arranged by size:
3678 1144 1011 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2815 3911 5121 2815
Size: 1096 1210 3713
Fragments arranged by size:
3713 1210 1096
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6019
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4630 136
Size: 107 122 71 631 605 2958 1525
Fragments arranged by size:
2958 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6014 6014
Size: 6019
BlpI GC'TnA_GC
Cuts at: 2615 3436 2615
Size: 821 5198
Bme1580I G_kGCm'C
Cuts at: 1790 2226 2844 3188 4080 5236 1790
Size: 436 618 344 892 1156 2573
Fragments arranged by size:
2573 1156 892 618 436 344
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6019
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3280 3750 4708
Size: 416 53 83 399 24 1863 470 958
Cuts at: 4708 442
Size: 1753
Fragments arranged by size:
1863 1753 958 470 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6019
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2542 4679 1239
Size: 1303 2137 2579
Fragments arranged by size:
2579 2137 1303
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4144 5009 5307 4144
Size: 865 298 4856
Fragments arranged by size:
4856 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2139 2204 3299 4682 882
Size: 617 640 65 1095 1383 2219
Fragments arranged by size:
2219 1383 1095 640 617 65
BsaAI yAC'GTr
Cuts at: 493 1204 2184 3810 493
Size: 711 980 1626 2702
Fragments arranged by size:
2702 1626 980 711
BsaBI GATnn'nnATC
Cuts at: 6013 6013
Size: 6019
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2166 3018 3255 3826
Size: 53 83 186 936 633 852 237 571
Cuts at: 3826 4208 4843 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 852 635 633 571 382 237
186 83 53
BsaWI w'CCGG_w
Cuts at: 2810 4452 4850 5124 5271 5682 2810
Size: 1642 398 274 147 411 3147
Fragments arranged by size:
3147 1642 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2199 2229 3260 3290 1178
Size: 30 165 30 796 30 1031 30 3907
Fragments arranged by size:
3907 1031 796 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3049 1186
Size: 1863 4156
BseYI C'CCAG_C
Cuts at: 2817 5222 5718 2817
Size: 2405 496 3118
Fragments arranged by size:
3118 2405 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2139 2450 2455 3170 1163
Size: 573 403 311 5 715 4012
Fragments arranged by size:
4012 715 573 403 311 5
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3469 4230 5258 1540
Size: 275 186 1468 761 1028 2301
Fragments arranged by size:
2301 1468 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4080 4165 5236 4080
Size: 85 1071 4863
Fragments arranged by size:
4863 1071 85
BsmI GAATG_Cn'
Cuts at: 2064 2081 3530 3623 2064
Size: 17 1449 93 4460
Fragments arranged by size:
4460 1449 93 17
BsmBI CGTCTCn'nnnn_
Cuts at: 2297 2297
Size: 6019
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2226 2679 2844 3188 3338 4080 4165
Size: 399 436 453 165 344 150 742 85
Cuts at: 4165 5236 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 453 436 399 344 165
150 85
BspEI T'CCGG_A
Cuts at: 5682 5682
Size: 6019
BspHI T'CATG_A
Cuts at: 3908 5638 3908
Size: 1730 4289
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2045 3374 844
Size: 1201 1329 3489
Fragments arranged by size:
3489 1329 1201
BsrBI CCG'CTC
Cuts at: 1394 1823 3906 1394
Size: 429 2083 3507
Fragments arranged by size:
3507 2083 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4514 4688 65
Size: 1183 3266 174 1396
Fragments arranged by size:
3266 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2810 3180 4663 4850 1700
Size: 110 1000 370 1483 187 2869
Fragments arranged by size:
2869 1483 1000 370 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6019
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6019
BssSI C'ACGA_G
Cuts at: 4079 5091 4079
Size: 1012 5007
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2783 794
Size: 1989 4030
BstBI TT'CG_AA
Cuts at: 4823 4823
Size: 6019
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6019
BstXI CCAn_nnnn'nTGG
Cuts at: 2598 2598
Size: 6019
BstYI r'GATC_y
Cuts at: 1972 3349 3716 4101 4118 5570 5656 5668 6014
Size: 1377 367 385 17 1452 86 12 346
Cuts at: 6014 1972
Size: 1977
Fragments arranged by size:
1977 1452 1377 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 3271 513
Size: 552 2206 3261
Fragments arranged by size:
3261 2206 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5280
Fragments arranged by size:
5280 683 56
BtsI GCAGTG_nn'
Cuts at: 2167 2467 2575 3158 3535 4329 4349 2167
Size: 300 108 583 377 794 20 3837
Fragments arranged by size:
3837 794 583 377 300 108 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6019
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5984
DraI TTT'AAA
Cuts at: 3323 3323
Size: 6019
DraIII CAC_nnn'GTG
Cuts at: 2839 2839
Size: 6019
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5026 809
Size: 507 3710 1802
Fragments arranged by size:
3710 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2646 2700 2787
Size: 54 1425 50 275 18 816 54 87
Cuts at: 2787 2982 3117 3466 4355 5984 8
Size: 195 135 349 889 1629 43
Fragments arranged by size:
1629 1425 889 816 349 275 195 135
87 54 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3466 1537
Size: 275 1654 4090
Fragments arranged by size:
4090 1654 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2459 3949 1353
Size: 1106 1490 3423
Fragments arranged by size:
3423 1490 1106
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4606 4980 5126 387
Size: 1064 138 3017 374 146 1280
Fragments arranged by size:
3017 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2224 2440 2542 3182 4082 4679 5466
Size: 393 592 216 102 640 900 597 787
Cuts at: 5466 5734 1239
Size: 268 1524
Fragments arranged by size:
1524 900 787 640 597 592 393 268
216 102
EcoO109I rG'GnC_Cy
Cuts at: 2752 3106 2752
Size: 354 5665
EcoRI G'AATT_C
Cuts at: 3328 3706 3328
Size: 378 5641
FspI TGC'GCA
Cuts at: 4525 4525
Size: 6019
HaeII r_GCGC'y
Cuts at: 1678 3258 5166 1678
Size: 1580 1908 2531
Fragments arranged by size:
2531 1908 1580
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2199 2231 2282 2314 2576
Size: 32 135 32 627 32 51 32 262
Cuts at: 2576 2608 2739 2771 4660 4692 4734 4766 1373
Size: 32 131 32 1889 32 42 32 2626
Fragments arranged by size:
2626 1889 627 262 135 131 51 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2934 3363 3609 669
Size: 988 281 996 429 246 3079
Fragments arranged by size:
3079 996 988 429 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6019
HpaI GTT'AAC
Cuts at: 2934 3609 2934
Size: 675 5344
KasI G'GCGC_C
Cuts at: 3254 3254
Size: 6019
KpnI G_GTAC'C
Cuts at: 3344 3344
Size: 6019
MfeI C'AATT_G
Cuts at: 3618 3618
Size: 6019
MluI A'CGCG_T
Cuts at: 5895 5895
Size: 6019
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5133 5317 1305
Size: 3828 184 2007
Fragments arranged by size:
3828 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2648 2702 2984 10
Size: 54 1425 343 816 54 282 3045
Fragments arranged by size:
3045 1425 816 343 282 54 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2528 3977 4336 4495 518
Size: 648 1362 1449 359 159 2042
Fragments arranged by size:
2042 1449 1362 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3010 3273 3435 4112 5260 5505 1606
Size: 1404 263 162 677 1148 245 2120
Fragments arranged by size:
2120 1404 1148 677 263 245 162
NarI GG'CG_CC
Cuts at: 3255 3255
Size: 6019
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5467
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6019
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6019
NotI GC'GGCC_GC
Cuts at: 3466 3466
Size: 6019
NspI r_CATG'y
Cuts at: 1132 1229 3377 4922 1132
Size: 97 2148 1545 2229
Fragments arranged by size:
2229 2148 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2398 1348
Size: 1050 4969
PciI A'CATG_T
Cuts at: 4918 4918
Size: 6019
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3028 3109 1108
Size: 1920 81 4018
Fragments arranged by size:
4018 1920 81
PmeI GTTT'AAAC
Cuts at: 3323 3323
Size: 6019
PmlI CAC'GTG
Cuts at: 2184 2184
Size: 6019
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4088 4120 5601 5633 4088
Size: 32 1481 32 4474
Fragments arranged by size:
4474 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3311 1613
Size: 1698 4321
PsiI TTA'TAA
Cuts at: 3589 3589
Size: 6019
PstI C_TGCA'G
Cuts at: 830 2162 2634 2928 3264 3371 830
Size: 1332 472 294 336 107 3478
Fragments arranged by size:
3478 1332 472 336 294 107
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6019
SacII CC_GC'GG
Cuts at: 3274 3274
Size: 6019
SalI G'TCGA_C
Cuts at: 1936 3361 1936
Size: 1425 4594
SbfI CC_TGCA'GG
Cuts at: 3371 3371
Size: 6019
ScaI AGT'ACT
Cuts at: 2636 4267 5979 2636
Size: 1631 1712 2676
Fragments arranged by size:
2676 1712 1631
SfcI C'TryA_G
Cuts at: 826 1045 2158 2335 2404 2630 2924 3260 3367
Size: 219 1113 177 69 226 294 336 107
Cuts at: 3367 4502 5183 5374 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1113 681 336 294 226 219
191 177 107 69
SfoI GGC'GCC
Cuts at: 3256 3256
Size: 6019
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6019
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6019
SmaI CCC'GGG
Cuts at: 1872 3346 1872
Size: 1474 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4123 5024 5286 820
Size: 197 926 2180 901 262 1553
Fragments arranged by size:
2180 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3810 493
Size: 3317 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6019
SphI G_CATG'C
Cuts at: 1132 3377 1132
Size: 2245 3774
SspI AAT'ATT
Cuts at: 5 52 3943 5
Size: 47 3891 2081
Fragments arranged by size:
3891 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6019
StyI C'CwwG_G
Cuts at: 513 1065 3458 513
Size: 552 2393 3074
Fragments arranged by size:
3074 2393 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2085 2590 3301 4046 4063 4216 4401
Size: 1255 505 505 711 745 17 153 185
Cuts at: 4401 4846 325
Size: 445 1498
Fragments arranged by size:
1498 1255 745 711 505 505 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2634 2970 4265
Size: 274 80 33 51 1443 657 336 1295
Cuts at: 4265 5977 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1295 657 336 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3260 3292 5979 6011 3260
Size: 32 2687 32 3268
Fragments arranged by size:
3268 2687 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3193 1316
Size: 1877 4142
XbaI T'CTAG_A
Cuts at: 3355 3355
Size: 6019
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2395 2530 2395
Size: 135 5884
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6019
XmaI C'CCGG_G
Cuts at: 1870 3344 1870
Size: 1474 4545
XmnI GAAnn'nnTTC
Cuts at: 2498 4148 2498
Size: 1650 4369
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3827 276
Size: 53 83 186 936 2293 2468
Fragments arranged by size:
2468 2293 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PstI PvuI SacII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SanDI SapI SexAI SfiI SrfI SwaI