Restriction map for
pFN21AE9524
(Circular) (Six-base) MAPSORT of: pFN21AE9524.seq Check: 3693 from: 1 to: 5749
pFN21AE9524 5749 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3559 278
Size: 53 83 186 936 2023 2468
Fragments arranged by size:
2468 2023 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3029 3092 1937
Size: 1092 63 4594
Fragments arranged by size:
4594 1092 63
Acc65I G'GTAC_C
Cuts at: 3070 3070
Size: 5749
AclI AA'CG_TT
Cuts at: 1563 3048 3876 4249 1563
Size: 1485 828 373 3063
Fragments arranged by size:
3063 1485 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2037 2422 3812 5196 5464 1632
Size: 405 385 1390 1384 268 1917
Fragments arranged by size:
1917 1390 1384 405 385 268
AfeI AGC'GCT
Cuts at: 2399 2399
Size: 5749
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5552
AflIII A'CryG_T
Cuts at: 1203 2229 4648 5625 1203
Size: 1026 2419 977 1327
Fragments arranged by size:
2419 1327 1026 977
AgeI A'CCGG_T
Cuts at: 4580 4580
Size: 5749
AhdI GACnn_n'nnGTC
Cuts at: 4478 4478
Size: 5749
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5749
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5749
AlwNI CAG_nnn'CTG
Cuts at: 2674 3492 5064 2674
Size: 818 1572 3359
Fragments arranged by size:
3359 1572 818
ApaI G_GGCC'C
Cuts at: 2683 2683
Size: 5749
ApaLI G'TGCA_C
Cuts at: 3806 4962 3806
Size: 1156 4593
ApoI r'AATT_y
Cuts at: 1493 3058 3284 3436 5545 1493
Size: 1565 226 152 2109 1697
Fragments arranged by size:
2109 1697 1565 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4303 160
Size: 872 3271 1606
Fragments arranged by size:
3271 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5749
AvaI C'yCGr_G
Cuts at: 1870 1943 3074 1870
Size: 73 1131 4545
Fragments arranged by size:
4545 1131 73
BamHI G'GATC_C
Cuts at: 2771 3079 3446 2771
Size: 308 367 5074
Fragments arranged by size:
5074 367 308
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2579 3070 4525 618
Size: 325 217 627 792 491 1455 1842
Fragments arranged by size:
1842 1455 792 627 491 325 217
BanII G_rGCy'C
Cuts at: 1391 2683 3068 1391
Size: 1292 385 4072
Fragments arranged by size:
4072 1292 385
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2128 2260 2269 2299 928
Size: 971 229 132 9 30 4378
Fragments arranged by size:
4378 971 229 132 30 9
BbvCI CC'TCA_GC
Cuts at: 2675 2675
Size: 5749
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3940 3974 1903
Size: 34 2003 34 3678
Fragments arranged by size:
3678 2003 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2291 3641 4851 2291
Size: 1350 1210 3189
Fragments arranged by size:
3189 1350 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5749
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2387 4360 136
Size: 107 122 71 631 605 715 1973 1525
Fragments arranged by size:
1973 1525 715 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5744 5744
Size: 5749
BlpI GC'TnA_GC
Cuts at: 2700 3166 2700
Size: 466 5283
Bme1580I G_kGCm'C
Cuts at: 1790 2132 2348 2582 2683 2796 3810 4966 1790
Size: 342 216 234 101 113 1014 1156 2573
Fragments arranged by size:
2573 1156 1014 342 234 216 113 101
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5749
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3480 4438 442
Size: 416 53 83 399 24 2063 958 1753
Fragments arranged by size:
2063 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5749
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2264 2264
Size: 5749
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2096 2539 4409 1239
Size: 857 443 1870 2579
Fragments arranged by size:
2579 1870 857 443
Bpu10I CC'TnA_GC
Cuts at: 2675 2675
Size: 5749
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3874 4739 5037 3874
Size: 865 298 4586
Fragments arranged by size:
4586 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4412 882
Size: 617 2913 2219
Fragments arranged by size:
2913 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2607 2791 3540 493
Size: 711 1403 184 749 2702
Fragments arranged by size:
2702 1403 749 711 184
BsaBI GATnn'nnATC
Cuts at: 5743 5743
Size: 5749
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2493 3556 3938 4573
Size: 53 83 186 936 960 1063 382 635
Cuts at: 4573 275
Size: 1451
Fragments arranged by size:
1451 1063 960 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4182 4580 4854 5001 5412 4182
Size: 398 274 147 411 4519
Fragments arranged by size:
4519 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2012 2042 1178
Size: 30 165 30 609 30 4885
Fragments arranged by size:
4885 609 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2240 1186
Size: 1054 4695
BseYI C'CCAG_C
Cuts at: 4952 5448 4952
Size: 496 5253
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5176
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2314 3199 3960 4988 1540
Size: 275 186 313 885 761 1028 2301
Fragments arranged by size:
2301 1028 885 761 313 275 186
BsiHKAI G_wGCw'C
Cuts at: 3810 3895 4966 3810
Size: 85 1071 4593
Fragments arranged by size:
4593 1071 85
BsmI GAATG_Cn'
Cuts at: 3260 3353 3260
Size: 93 5656
BsmBI CGTCTCn'nnnn_
Cuts at: 2500 2500
Size: 5749
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2132 2348 2582 2683 2796 3068 3810
Size: 399 342 216 234 101 113 272 742
Cuts at: 3810 3895 4966 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 342 272 234 216
113 101 85
BspEI T'CCGG_A
Cuts at: 5412 5412
Size: 5749
BspHI T'CATG_A
Cuts at: 2919 2946 3638 5368 2919
Size: 27 692 1730 3300
Fragments arranged by size:
3300 1730 692 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2345 3104 844
Size: 1501 759 3489
Fragments arranged by size:
3489 1501 759
BsrBI CCG'CTC
Cuts at: 1394 1823 3636 1394
Size: 429 1813 3507
Fragments arranged by size:
3507 1813 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2085 4244 4418 65
Size: 1183 837 2159 174 1396
Fragments arranged by size:
2159 1396 1183 837 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2523 4393 4580 1700
Size: 110 713 1870 187 2869
Fragments arranged by size:
2869 1870 713 187 110
BsrGI T'GTAC_A
Cuts at: 96 2814 96
Size: 2718 3031
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5749
BssSI C'ACGA_G
Cuts at: 3809 4821 3809
Size: 1012 4737
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5749
BstBI TT'CG_AA
Cuts at: 4553 4553
Size: 5749
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5749
BstXI CCAn_nnnn'nTGG
Cuts at: 2170 2818 2170
Size: 648 5101
BstYI r'GATC_y
Cuts at: 1972 2771 3079 3446 3831 3848 5300 5386 5398
Size: 799 308 367 385 17 1452 86 12
Cuts at: 5398 5744 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 799 385 367 346 308 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2535 2535
Size: 5749
BtgI C'CryG_G
Cuts at: 513 1065 2063 2163 2574 2836 513
Size: 552 998 100 411 262 3426
Fragments arranged by size:
3426 998 552 411 262 100
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5010
Fragments arranged by size:
5010 683 56
BtsI GCAGTG_nn'
Cuts at: 2180 2552 3265 4059 4079 2180
Size: 372 713 794 20 3850
Fragments arranged by size:
3850 794 713 372 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5749
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5714
DraI TTT'AAA
Cuts at: 3053 3053
Size: 5749
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4756 809
Size: 507 3440 1802
Fragments arranged by size:
3440 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2175 3196 4085
Size: 54 1425 50 275 18 345 1021 889
Cuts at: 4085 5714 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1021 889 345 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3196 1537
Size: 275 1384 4090
Fragments arranged by size:
4090 1384 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2019 2056 2621 3679 1353
Size: 666 37 565 1058 3423
Fragments arranged by size:
3423 1058 666 565 37
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4336 4710 4856 387
Size: 1064 138 2747 374 146 1280
Fragments arranged by size:
2747 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2037 2096 2422 2539 3812 4409 5196
Size: 393 405 59 326 117 1273 597 787
Cuts at: 5196 5464 1239
Size: 268 1524
Fragments arranged by size:
1524 1273 787 597 405 393 326 268
117 59
EcoO109I rG'GnC_Cy
Cuts at: 2539 2679 2680 2539
Size: 140 1 5608
Fragments arranged by size:
5608 140 1
EcoRI G'AATT_C
Cuts at: 3058 3436 3058
Size: 378 5371
FspI TGC'GCA
Cuts at: 4255 4255
Size: 5749
HaeII r_GCGC'y
Cuts at: 1678 2401 4896 1678
Size: 723 2495 2531
Fragments arranged by size:
2531 2495 723
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2289 2321 4390 4422 4464
Size: 32 135 32 717 32 2069 32 42
Cuts at: 4464 4496 1373
Size: 32 2626
Fragments arranged by size:
2626 2069 717 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2227 2824 3093 3339 669
Size: 988 281 289 597 269 246 3079
Fragments arranged by size:
3079 988 597 289 281 269 246
HindIII A'AGCT_T
Cuts at: 748 2937 748
Size: 2189 3560
HpaI GTT'AAC
Cuts at: 3339 3339
Size: 5749
KpnI G_GTAC'C
Cuts at: 3074 3074
Size: 5749
MfeI C'AATT_G
Cuts at: 3348 3348
Size: 5749
MluI A'CGCG_T
Cuts at: 5625 5625
Size: 5749
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1998 3009 4863 5047 1305
Size: 693 1011 1854 184 2007
Fragments arranged by size:
2007 1854 1011 693 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2177 10
Size: 54 1425 343 345 3582
Fragments arranged by size:
3582 1425 345 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2322 2816 2951 3707 4066 4225 518
Size: 648 1156 494 135 756 359 159 2042
Fragments arranged by size:
2042 1156 756 648 494 359 159 135
MspA1I CmG'CkG
Cuts at: 1606 2289 2671 3165 3842 4990 5235 1606
Size: 683 382 494 677 1148 245 2120
Fragments arranged by size:
2120 1148 683 677 494 382 245
NcoI C'CATG_G
Cuts at: 513 1065 2163 2836 513
Size: 552 1098 673 3426
Fragments arranged by size:
3426 1098 673 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5749
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5749
NotI GC'GGCC_GC
Cuts at: 3196 3196
Size: 5749
NspI r_CATG'y
Cuts at: 1132 1229 2453 2994 3107 4652 1132
Size: 97 1224 541 113 1545 2229
Fragments arranged by size:
2229 1545 1224 541 113 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5749
PciI A'CATG_T
Cuts at: 4648 4648
Size: 5749
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2842 1108
Size: 1734 4015
PmeI GTTT'AAAC
Cuts at: 3053 3053
Size: 5749
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3818 3850 5331 5363 3818
Size: 32 1481 32 4204
Fragments arranged by size:
4204 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2462 1613
Size: 849 4900
PsiI TTA'TAA
Cuts at: 3319 3319
Size: 5749
PspOMI G'GGCC_C
Cuts at: 2679 2679
Size: 5749
PstI C_TGCA'G
Cuts at: 830 2547 3101 830
Size: 1717 554 3478
Fragments arranged by size:
3478 1717 554
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5749
PvuII CAG'CTG
Cuts at: 2289 2671 2289
Size: 382 5367
SalI G'TCGA_C
Cuts at: 1936 3091 1936
Size: 1155 4594
SbfI CC_TGCA'GG
Cuts at: 3101 3101
Size: 5749
ScaI AGT'ACT
Cuts at: 3997 5709 3997
Size: 1712 4037
SfcI C'TryA_G
Cuts at: 826 1045 2349 2543 3097 4232 4913 5104 826
Size: 219 1304 194 554 1135 681 191 1471
Fragments arranged by size:
1471 1304 1135 681 554 219 194 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2387 2387
Size: 5749
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5749
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5749
SmaI CCC'GGG
Cuts at: 1872 3076 1872
Size: 1204 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3853 4754 5016 820
Size: 197 926 1910 901 262 1553
Fragments arranged by size:
1910 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3540 493
Size: 3047 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5749
SphI G_CATG'C
Cuts at: 1132 2453 3107 1132
Size: 1321 654 3774
Fragments arranged by size:
3774 1321 654
SspI AAT'ATT
Cuts at: 5 52 3673 5
Size: 47 3621 2081
Fragments arranged by size:
3621 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5749
StyI C'CwwG_G
Cuts at: 513 1065 2163 2836 3188 513
Size: 552 1098 673 352 3074
Fragments arranged by size:
3074 1098 673 552 352
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2300 3776 3793 3946 4131 4576 325
Size: 1255 720 1476 17 153 185 445 1498
Fragments arranged by size:
1498 1476 1255 720 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2814 3995 5707
Size: 274 80 33 51 1443 837 1181 1712
Cuts at: 5707 96
Size: 138
Fragments arranged by size:
1712 1443 1181 837 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2367 2399 5709 5741 2367
Size: 32 3310 32 2375
Fragments arranged by size:
3310 2375 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5749
XbaI T'CTAG_A
Cuts at: 3085 3085
Size: 5749
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2170 2170
Size: 5749
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5749
XmaI C'CCGG_G
Cuts at: 1870 3074 1870
Size: 1204 4545
XmnI GAAnn'nnTTC
Cuts at: 3878 3878
Size: 5749
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3557 276
Size: 53 83 186 936 2023 2468
Fragments arranged by size:
2468 2023 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfoI SrfI SwaI