Restriction map for
pFN21AE9521
(Circular) (Six-base) MAPSORT of: pFN21AE9521.seq Check: 1088 from: 1 to: 5656
pFN21AE9521 5656 bp
With 182 enzymes: SgfI *
January 30, 2014 17:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2384 2384
Size: 5656
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3466 278
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2999 1937
Size: 1062 4594
Acc65I G'GTAC_C
Cuts at: 2977 2977
Size: 5656
AclI AA'CG_TT
Cuts at: 1563 3783 4156 1563
Size: 2220 373 3063
Fragments arranged by size:
3063 2220 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2512 3719 5103 5371 1632
Size: 880 1207 1384 268 1917
Fragments arranged by size:
1917 1384 1207 880 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5459
AflIII A'CryG_T
Cuts at: 1203 4555 5532 1203
Size: 3352 977 1327
Fragments arranged by size:
3352 1327 977
AgeI A'CCGG_T
Cuts at: 4487 4487
Size: 5656
AhdI GACnn_n'nnGTC
Cuts at: 4385 4385
Size: 5656
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5656
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2402 1184
Size: 1218 4438
AlwNI CAG_nnn'CTG
Cuts at: 2350 2400 3399 4971 2350
Size: 50 999 1572 3035
Fragments arranged by size:
3035 1572 999 50
ApaI G_GGCC'C
Cuts at: 2771 2771
Size: 5656
ApaLI G'TGCA_C
Cuts at: 2316 3713 4869 2316
Size: 1397 1156 3103
Fragments arranged by size:
3103 1397 1156
ApoI r'AATT_y
Cuts at: 1493 2965 3191 3343 5452 1493
Size: 1472 226 152 2109 1697
Fragments arranged by size:
2109 1697 1472 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4210 160
Size: 872 3178 1606
Fragments arranged by size:
3178 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
AvaI C'yCGr_G
Cuts at: 1870 1943 2328 2640 2807 2981 1870
Size: 73 385 312 167 174 4545
Fragments arranged by size:
4545 385 312 174 167 73
BamHI G'GATC_C
Cuts at: 2018 2986 3353 2018
Size: 968 367 4321
Fragments arranged by size:
4321 968 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2106 2411 2977 4432 618
Size: 325 217 627 319 305 566 1455 1842
Fragments arranged by size:
1842 1455 627 566 325 319 305 217
BanII G_rGCy'C
Cuts at: 1391 2042 2104 2136 2771 2919 2975 1391
Size: 651 62 32 635 148 56 4072
Fragments arranged by size:
4072 651 635 148 62 56 32
BbeI G_GCGC'C
Cuts at: 2415 2415
Size: 5656
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2035 2518 2578 928
Size: 971 136 483 60 4006
Fragments arranged by size:
4006 971 483 136 60
BbvCI CC'TCA_GC
Cuts at: 2141 2141
Size: 5656
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3847 3881 1903
Size: 34 1910 34 3678
Fragments arranged by size:
3678 1910 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3548 4758 3548
Size: 1210 4446
BclI T'GATC_A
Cuts at: 1548 2370 1548
Size: 822 4834
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2501 2718 4267
Size: 107 122 71 631 605 829 217 1549
Cuts at: 4267 136
Size: 1525
Fragments arranged by size:
1549 1525 829 631 605 217 122 107
71
BglII A'GATC_T
Cuts at: 5651 5651
Size: 5656
BlpI GC'TnA_GC
Cuts at: 3073 3073
Size: 5656
Bme1580I G_kGCm'C
Cuts at: 1790 2320 2771 3717 4873 1790
Size: 530 451 946 1156 2573
Fragments arranged by size:
2573 1156 946 530 451
BmgBI CAC'GTC
Cuts at: 1318 2368 1318
Size: 1050 4606
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3387 4345 442
Size: 416 53 83 399 24 1970 958 1753
Fragments arranged by size:
1970 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5656
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2132 2303 2327 4316 1239
Size: 893 171 24 1989 2579
Fragments arranged by size:
2579 1989 893 171 24
Bpu10I CC'TnA_GC
Cuts at: 2141 2141
Size: 5656
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2915 3781 4646 4944 2915
Size: 866 865 298 3627
Fragments arranged by size:
3627 866 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4319 882
Size: 617 2820 2219
Fragments arranged by size:
2820 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2560 2820 3447 493
Size: 711 1356 260 627 2702
Fragments arranged by size:
2702 1356 711 627 260
BsaBI GATnn'nnATC
Cuts at: 5650 5650
Size: 5656
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2412 3463 3845 4480
Size: 53 83 186 936 879 1051 382 635
Cuts at: 4480 275
Size: 1451
Fragments arranged by size:
1451 1051 936 879 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2202 4089 4487 4761 4908 5319 2202
Size: 1887 398 274 147 411 2539
Fragments arranged by size:
2539 1887 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5431
Fragments arranged by size:
5431 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5656
BseYI C'CCAG_C
Cuts at: 2070 2074 2743 2763 4859 5355 2070
Size: 4 669 20 2096 496 2371
Fragments arranged by size:
2371 2096 669 496 20 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2033 2195 1163
Size: 573 297 162 4624
Fragments arranged by size:
4624 573 297 162
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2292 3106 3867 4895 1540
Size: 275 186 291 814 761 1028 2301
Fragments arranged by size:
2301 1028 814 761 291 275 186
BsiHKAI G_wGCw'C
Cuts at: 2320 2610 2814 2919 3717 3802 4873 2320
Size: 290 204 105 798 85 1071 3103
Fragments arranged by size:
3103 1071 798 290 204 105 85
BsmI GAATG_Cn'
Cuts at: 3167 3260 3167
Size: 93 5563
BsmBI CGTCTCn'nnnn_
Cuts at: 2279 2279
Size: 5656
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2042 2104 2136 2320 2610 2771 2814
Size: 399 252 62 32 184 290 161 43
Cuts at: 2814 2919 2975 3717 3802 4873 1391
Size: 105 56 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 290 252 184 161
105 85 62 56 43 32
BspEI T'CCGG_A
Cuts at: 5319 5319
Size: 5656
BspHI T'CATG_A
Cuts at: 3545 5275 3545
Size: 1730 3926
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2217 2265 2384 3011 844
Size: 1373 48 119 627 3489
Fragments arranged by size:
3489 1373 627 119 48
BsrBI CCG'CTC
Cuts at: 1394 1823 2268 3543 1394
Size: 429 445 1275 3507
Fragments arranged by size:
3507 1275 445 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2878 4151 4325 65
Size: 1183 1630 1273 174 1396
Fragments arranged by size:
1630 1396 1273 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2312 4300 4487 1700
Size: 110 502 1988 187 2869
Fragments arranged by size:
2869 1988 502 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5656
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5656
BssSI C'ACGA_G
Cuts at: 2579 3716 4728 2579
Size: 1137 1012 3507
Fragments arranged by size:
3507 1137 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5656
BstBI TT'CG_AA
Cuts at: 4460 4460
Size: 5656
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5656
BstXI CCAn_nnnn'nTGG
Cuts at: 2422 2512 2708 2743 2422
Size: 90 196 35 5335
Fragments arranged by size:
5335 196 90 35
BstYI r'GATC_y
Cuts at: 1972 2018 2986 3353 3738 3755 5207 5293 5305
Size: 46 968 367 385 17 1452 86 12
Cuts at: 5305 5651 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 968 385 367 346 86 46
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2421 2480 513
Size: 552 939 417 59 3689
Fragments arranged by size:
3689 939 552 417 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2176 2401 2527 2650 487
Size: 683 56 950 225 126 123 3493
Fragments arranged by size:
3493 950 683 225 126 123 56
BtsI GCAGTG_nn'
Cuts at: 2450 3172 3966 3986 2450
Size: 722 794 20 4120
Fragments arranged by size:
4120 794 722 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5656
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5621
DraI TTT'AAA
Cuts at: 2960 2960
Size: 5656
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4663 809
Size: 507 3347 1802
Fragments arranged by size:
3347 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2184 2289 2502
Size: 54 1425 50 275 18 354 105 213
Cuts at: 2502 2514 2562 2613 3103 3992 5621 8
Size: 12 48 51 490 889 1629 43
Fragments arranged by size:
1629 1425 889 490 354 275 213 105
54 51 50 48 43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2289 3103 1537
Size: 275 477 814 4090
Fragments arranged by size:
4090 814 477 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2141 2543 3586 1353
Size: 788 402 1043 3423
Fragments arranged by size:
3423 1043 788 402
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4243 4617 4763 387
Size: 1064 138 2654 374 146 1280
Fragments arranged by size:
2654 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2917 2917
Size: 5656
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2132 2303 2327 2512 3719 4316 5103
Size: 393 500 171 24 185 1207 597 787
Cuts at: 5103 5371 1239
Size: 268 1524
Fragments arranged by size:
1524 1207 787 597 500 393 268 185
171 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2306 2306
Size: 5656
EcoO109I rG'GnC_Cy
Cuts at: 2767 2768 2767
Size: 1 5655
EcoRI G'AATT_C
Cuts at: 2965 3343 2965
Size: 378 5278
FspI TGC'GCA
Cuts at: 4162 4162
Size: 5656
HaeII r_GCGC'y
Cuts at: 1678 2415 2692 2724 4803 1678
Size: 737 277 32 2079 2531
Fragments arranged by size:
2531 2079 737 277 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4297 4329 4371 4403 1373
Size: 32 135 32 2725 32 42 32 2626
Fragments arranged by size:
2725 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3000 3246 669
Size: 988 281 1062 246 3079
Fragments arranged by size:
3079 1062 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5656
HpaI GTT'AAC
Cuts at: 3246 3246
Size: 5656
KasI G'GCGC_C
Cuts at: 2411 2411
Size: 5656
KpnI G_GTAC'C
Cuts at: 2981 2981
Size: 5656
MfeI C'AATT_G
Cuts at: 3255 3255
Size: 5656
MluI A'CGCG_T
Cuts at: 5532 5532
Size: 5656
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2232 4770 4954 1305
Size: 927 2538 184 2007
Fragments arranged by size:
2538 2007 927 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2186 2516 10
Size: 54 1425 343 354 330 3150
Fragments arranged by size:
3150 1425 354 343 330 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2366 2420 2510 2615 3614 3973
Size: 648 843 357 54 90 105 999 359
Cuts at: 3973 4132 518
Size: 159 2042
Fragments arranged by size:
2042 999 843 648 359 357 159 105
90 54
MspA1I CmG'CkG
Cuts at: 1606 2074 2350 2482 3072 3749 4897 5142 1606
Size: 468 276 132 590 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 590 468 276 245 132
NarI GG'CG_CC
Cuts at: 2412 2412
Size: 5656
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4165
Fragments arranged by size:
4165 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5656
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5656
NotI GC'GGCC_GC
Cuts at: 3103 3103
Size: 5656
NspI r_CATG'y
Cuts at: 1132 1229 2635 2949 3014 4559 1132
Size: 97 1406 314 65 1545 2229
Fragments arranged by size:
2229 1545 1406 314 97 65
PasI CC'CwG_GG
Cuts at: 1348 2659 1348
Size: 1311 4345
PciI A'CATG_T
Cuts at: 4555 4555
Size: 5656
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2635 1108
Size: 1527 4129
PmeI GTTT'AAAC
Cuts at: 2960 2960
Size: 5656
PmlI CAC'GTG
Cuts at: 2560 2820 2560
Size: 260 5396
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2198 2230 3725 3757 5238 5270 2198
Size: 32 1495 32 1481 32 2584
Fragments arranged by size:
2584 1495 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2291 1613
Size: 678 4978
PsiI TTA'TAA
Cuts at: 3226 3226
Size: 5656
PspOMI G'GGCC_C
Cuts at: 2767 2767
Size: 5656
PstI C_TGCA'G
Cuts at: 830 2346 2381 3008 830
Size: 1516 35 627 3478
Fragments arranged by size:
3478 1516 627 35
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5656
PvuII CAG'CTG
Cuts at: 2074 2350 2074
Size: 276 5380
SacI G_AGCT'C
Cuts at: 2919 2919
Size: 5656
SacII CC_GC'GG
Cuts at: 2483 2483
Size: 5656
SalI G'TCGA_C
Cuts at: 1936 2998 1936
Size: 1062 4594
SapI GCTCTTCn'nnn_
Cuts at: 2141 2543 2141
Size: 402 5254
SbfI CC_TGCA'GG
Cuts at: 3008 3008
Size: 5656
ScaI AGT'ACT
Cuts at: 3904 5616 3904
Size: 1712 3944
SfcI C'TryA_G
Cuts at: 826 1045 2342 2377 3004 4139 4820 5011 826
Size: 219 1297 35 627 1135 681 191 1471
Fragments arranged by size:
1471 1297 1135 681 627 219 191 35
SfoI GGC'GCC
Cuts at: 2413 2413
Size: 5656
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5656
SmaI CCC'GGG
Cuts at: 1872 2330 2642 2983 1872
Size: 458 312 341 4545
Fragments arranged by size:
4545 458 341 312
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2930 3760 4661 4923 820
Size: 197 926 987 830 901 262 1553
Fragments arranged by size:
1553 987 926 901 830 262 197
SnaBI TAC'GTA
Cuts at: 493 3447 493
Size: 2954 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5656
SphI G_CATG'C
Cuts at: 1132 3014 1132
Size: 1882 3774
SrfI GCCC'GGGC
Cuts at: 2330 2642 2330
Size: 312 5344
SspI AAT'ATT
Cuts at: 5 52 3580 5
Size: 47 3528 2081
Fragments arranged by size:
3528 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5656
StyI C'CwwG_G
Cuts at: 513 1065 2004 3095 513
Size: 552 939 1091 3074
Fragments arranged by size:
3074 1091 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2233 2500 3683 3700 3853 4038 4483
Size: 1255 653 267 1183 17 153 185 445
Cuts at: 4483 325
Size: 1498
Fragments arranged by size:
1498 1255 1183 653 445 267 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2223 3902 5614
Size: 274 80 33 51 1443 246 1679 1712
Cuts at: 5614 96
Size: 138
Fragments arranged by size:
1712 1679 1443 274 246 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2781 2813 5616 5648 2781
Size: 32 2803 32 2789
Fragments arranged by size:
2803 2789 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2357 1316
Size: 1041 4615
XbaI T'CTAG_A
Cuts at: 2992 2992
Size: 5656
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2071 2705 2071
Size: 634 5022
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5656
XmaI C'CCGG_G
Cuts at: 1870 2328 2640 2981 1870
Size: 458 312 341 4545
Fragments arranged by size:
4545 458 341 312
XmnI GAAnn'nnTTC
Cuts at: 3785 3785
Size: 5656
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3464 276
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PfoI PpuMI PspXI PsrI
RsrII SanDI SexAI SfiI SwaI