Restriction map for
pFN21AE9520
(Circular) (Six-base) MAPSORT of: pFN21AE9520.seq Check: 5450 from: 1 to: 6040
pFN21AE9520 6040 bp
With 182 enzymes: SgfI *
March 28, 2012 12:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2052 2248 2427 2052
Size: 196 179 5665
Fragments arranged by size:
5665 196 179
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3850 278
Size: 53 83 186 936 2314 2468
Fragments arranged by size:
2468 2314 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3383 1937
Size: 1446 4594
Acc65I G'GTAC_C
Cuts at: 3361 3361
Size: 6040
AclI AA'CG_TT
Cuts at: 1563 4167 4540 1563
Size: 2604 373 3063
Fragments arranged by size:
3063 2604 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2607 4103 5487 5755 1632
Size: 975 1496 1384 268 1917
Fragments arranged by size:
1917 1496 1384 975 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5843
AflIII A'CryG_T
Cuts at: 1203 3297 4939 5916 1203
Size: 2094 1642 977 1327
Fragments arranged by size:
2094 1642 1327 977
AgeI A'CCGG_T
Cuts at: 4871 4871
Size: 6040
AhdI GACnn_n'nnGTC
Cuts at: 4769 4769
Size: 6040
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6040
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2300 2392 1184
Size: 1116 92 4832
Fragments arranged by size:
4832 1116 92
AlwNI CAG_nnn'CTG
Cuts at: 2263 2630 2835 3783 5355 2263
Size: 367 205 948 1572 2948
Fragments arranged by size:
2948 1572 948 367 205
ApaLI G'TGCA_C
Cuts at: 4097 5253 4097
Size: 1156 4884
ApoI r'AATT_y
Cuts at: 1493 3349 3575 3727 5836 1493
Size: 1856 226 152 2109 1697
Fragments arranged by size:
2109 1856 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4594 160
Size: 872 3562 1606
Fragments arranged by size:
3562 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6040
AvaI C'yCGr_G
Cuts at: 1870 1943 2513 3365 1870
Size: 73 570 852 4545
Fragments arranged by size:
4545 852 570 73
BamHI G'GATC_C
Cuts at: 3370 3737 3370
Size: 367 5673
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2301 2540 2780 3104 3361
Size: 325 217 627 514 239 240 324 257
Cuts at: 3361 4816 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 514 325 324 257 240
239 217
BanII G_rGCy'C
Cuts at: 1391 2072 2716 3086 3251 3359 1391
Size: 681 644 370 165 108 4072
Fragments arranged by size:
4072 681 644 370 165 108
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2636 928
Size: 971 737 4332
Fragments arranged by size:
4332 971 737
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4231 4265 1903
Size: 34 2294 34 3678
Fragments arranged by size:
3678 2294 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3932 5142 3932
Size: 1210 4830
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6040
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2288 2318 2844
Size: 107 122 71 631 605 616 30 526
Cuts at: 2844 3233 4651 136
Size: 389 1418 1525
Fragments arranged by size:
1525 1418 631 616 605 526 389 122
107 71 30
BglII A'GATC_T
Cuts at: 6035 6035
Size: 6040
BlpI GC'TnA_GC
Cuts at: 3457 3457
Size: 6040
Bme1580I G_kGCm'C
Cuts at: 1790 2279 2470 2522 2602 3107 4101 5257 1790
Size: 489 191 52 80 505 994 1156 2573
Fragments arranged by size:
2573 1156 994 505 489 191 80 52
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6040
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2309 3771 4729
Size: 416 53 83 399 24 892 1462 958
Cuts at: 4729 442
Size: 1753
Fragments arranged by size:
1753 1462 958 892 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6040
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2227 2687 4700 1239
Size: 988 460 2013 2579
Fragments arranged by size:
2579 2013 988 460
Bpu10I CC'TnA_GC
Cuts at: 3080 3080
Size: 6040
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2431 2588 4165 5030 5328 2431
Size: 157 1577 865 298 3143
Fragments arranged by size:
3143 1577 865 298 157
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4703 882
Size: 617 3204 2219
Fragments arranged by size:
3204 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3169 3298 3831 493
Size: 711 1965 129 533 2702
Fragments arranged by size:
2702 1965 711 533 129
BsaBI GATnn'nnATC
Cuts at: 6034 6034
Size: 6040
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2923 3847 4229 4864
Size: 53 83 186 936 1390 924 382 635
Cuts at: 4864 275
Size: 1451
Fragments arranged by size:
1451 1390 936 924 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2029 4473 4871 5145 5292 5703 2029
Size: 2444 398 274 147 411 2366
Fragments arranged by size:
2444 2366 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5815
Fragments arranged by size:
5815 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2224 1186
Size: 1038 5002
BseYI C'CCAG_C
Cuts at: 2263 2842 2950 3019 3037 5243 5739 2263
Size: 579 108 69 18 2206 496 2564
Fragments arranged by size:
2564 2206 579 496 108 69 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2276 2516 2915 3022 3342 1163
Size: 573 540 240 399 107 320 3861
Fragments arranged by size:
3861 573 540 399 320 240 107
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3212 3490 4251 5279 1540
Size: 275 186 1211 278 761 1028 2301
Fragments arranged by size:
2301 1211 1028 761 278 275 186
BsiHKAI G_wGCw'C
Cuts at: 4101 4186 5257 4101
Size: 85 1071 4884
Fragments arranged by size:
4884 1071 85
BsmI GAATG_Cn'
Cuts at: 2332 3551 3644 2332
Size: 1219 93 4728
Fragments arranged by size:
4728 1219 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2161 2161
Size: 6040
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2072 2279 2470 2522 2602 2716 3086
Size: 399 282 207 191 52 80 114 370
Cuts at: 3086 3107 3251 3359 4101 4186 5257 1391
Size: 21 144 108 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 370 282 207 191
144 114 108 85 80 52 21
BspEI T'CCGG_A
Cuts at: 5703 5703
Size: 6040
BspHI T'CATG_A
Cuts at: 3929 5659 3929
Size: 1730 4310
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2052 2227 2248 2427 2994 3395 844
Size: 1208 175 21 179 567 401 3489
Fragments arranged by size:
3489 1208 567 401 179 175 21
BsrBI CCG'CTC
Cuts at: 1394 1823 2557 3927 1394
Size: 429 734 1370 3507
Fragments arranged by size:
3507 1370 734 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3215 4535 4709 65
Size: 1183 1967 1320 174 1396
Fragments arranged by size:
1967 1396 1320 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3207 3211 3227 4684 4871 1700
Size: 110 1397 4 16 1457 187 2869
Fragments arranged by size:
2869 1457 1397 187 110 16 4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6040
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6040
BssSI C'ACGA_G
Cuts at: 3125 4100 5112 3125
Size: 975 1012 4053
Fragments arranged by size:
4053 1012 975
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2381 2606 2835 794
Size: 1587 225 229 3999
Fragments arranged by size:
3999 1587 229 225
BstBI TT'CG_AA
Cuts at: 4844 4844
Size: 6040
BstEII G'GTnAC_C
Cuts at: 1184 2197 1184
Size: 1013 5027
BstXI CCAn_nnnn'nTGG
Cuts at: 2136 2251 2971 3244 2136
Size: 115 720 273 4932
Fragments arranged by size:
4932 720 273 115
BstYI r'GATC_y
Cuts at: 1972 2091 3370 3737 4122 4139 5591 5677 5689
Size: 119 1279 367 385 17 1452 86 12
Cuts at: 5689 6035 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1279 385 367 346 119 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2802 2802
Size: 6040
BtgI C'CryG_G
Cuts at: 513 1065 2004 2988 513
Size: 552 939 984 3565
Fragments arranged by size:
3565 984 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5301
Fragments arranged by size:
5301 683 56
BtsI GCAGTG_nn'
Cuts at: 2750 2836 2962 3556 4350 4370 2750
Size: 86 126 594 794 20 4420
Fragments arranged by size:
4420 794 594 126 86 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6040
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6005
DraI TTT'AAA
Cuts at: 3344 3344
Size: 6040
DraIII CAC_nnn'GTG
Cuts at: 2308 2308
Size: 6040
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2237 5047 809
Size: 507 921 2810 1802
Fragments arranged by size:
2810 1802 921 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2853 2985 3209
Size: 54 1425 50 275 18 1023 132 224
Cuts at: 3209 3234 3487 4376 6005 8
Size: 25 253 889 1629 43
Fragments arranged by size:
1629 1425 1023 889 275 253 224 132
54 50 43 25 18
EagI C'GGCC_G
Cuts at: 1537 1812 3209 3487 1537
Size: 275 1397 278 4090
Fragments arranged by size:
4090 1397 278 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2063 2724 2733 3970 1353
Size: 710 661 9 1237 3423
Fragments arranged by size:
3423 1237 710 661 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4627 5001 5147 387
Size: 1064 138 3038 374 146 1280
Fragments arranged by size:
3038 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2227 2607 2687 4103 4700 5487 5755
Size: 393 595 380 80 1416 597 787 268
Cuts at: 5755 1239
Size: 1524
Fragments arranged by size:
1524 1416 787 597 595 393 380 268
80
EcoNI CCTnn'n_nnAGG
Cuts at: 2495 2495
Size: 6040
EcoO109I rG'GnC_Cy
Cuts at: 2239 3006 2239
Size: 767 5273
EcoRI G'AATT_C
Cuts at: 3349 3727 3349
Size: 378 5662
FseI GG_CCGG'CC
Cuts at: 3211 3211
Size: 6040
FspI TGC'GCA
Cuts at: 4546 4546
Size: 6040
HaeII r_GCGC'y
Cuts at: 1678 3071 3264 5187 1678
Size: 1393 193 1923 2531
Fragments arranged by size:
2531 1923 1393 193
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2678 2685 2710 2717 4681
Size: 32 135 32 1106 7 25 7 1964
Cuts at: 4681 4713 4755 4787 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1964 1106 135 42 32 32 32
32 25 7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 3384 3630 669
Size: 988 281 1446 246 3079
Fragments arranged by size:
3079 1446 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6040
HpaI GTT'AAC
Cuts at: 3630 3630
Size: 6040
KpnI G_GTAC'C
Cuts at: 3365 3365
Size: 6040
MfeI C'AATT_G
Cuts at: 3639 3639
Size: 6040
MluI A'CGCG_T
Cuts at: 5916 5916
Size: 6040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2117 5154 5338 1305
Size: 812 3037 184 2007
Fragments arranged by size:
3037 2007 812 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2987 3236 10
Size: 54 1425 343 1155 249 2814
Fragments arranged by size:
2814 1425 1155 343 249 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2249 2374 2993 3242 3998 4357 4516
Size: 648 1083 125 619 249 756 359 159
Cuts at: 4516 518
Size: 2042
Fragments arranged by size:
2042 1083 756 648 619 359 249 159
125
MspA1I CmG'CkG
Cuts at: 1606 2627 3456 4133 5281 5526 1606
Size: 1021 829 677 1148 245 2120
Fragments arranged by size:
2120 1148 1021 829 677 245
NaeI GCC'GGC
Cuts at: 3209 3213 3229 3209
Size: 4 16 6020
Fragments arranged by size:
6020 16 4
NcoI C'CATG_G
Cuts at: 513 1065 2004 2988 513
Size: 552 939 984 3565
Fragments arranged by size:
3565 984 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6040
NgoMIV G'CCGG_C
Cuts at: 3207 3211 3227 3207
Size: 4 16 6020
Fragments arranged by size:
6020 16 4
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6040
NotI GC'GGCC_GC
Cuts at: 3487 3487
Size: 6040
NspI r_CATG'y
Cuts at: 1132 1229 2379 3398 4943 1132
Size: 97 1150 1019 1545 2229
Fragments arranged by size:
2229 1545 1150 1019 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6040
PciI A'CATG_T
Cuts at: 4939 4939
Size: 6040
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2047 1108
Size: 939 5101
PmeI GTTT'AAAC
Cuts at: 3344 3344
Size: 6040
PmlI CAC'GTG
Cuts at: 3298 3298
Size: 6040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4109 4141 5622 5654 4109
Size: 32 1481 32 4495
Fragments arranged by size:
4495 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2239 3006 2239
Size: 767 5273
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6040
PsiI TTA'TAA
Cuts at: 3610 3610
Size: 6040
PspXI vC'TCGA_Gb
Cuts at: 2513 2513
Size: 6040
PstI C_TGCA'G
Cuts at: 830 3392 830
Size: 2562 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6040
PvuII CAG'CTG
Cuts at: 2627 2627
Size: 6040
SalI G'TCGA_C
Cuts at: 1936 3382 1936
Size: 1446 4594
SbfI CC_TGCA'GG
Cuts at: 3392 3392
Size: 6040
ScaI AGT'ACT
Cuts at: 4288 6000 4288
Size: 1712 4328
SfcI C'TryA_G
Cuts at: 826 1045 3388 4523 5204 5395 826
Size: 219 2343 1135 681 191 1471
Fragments arranged by size:
2343 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6040
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6040
SmaI CCC'GGG
Cuts at: 1872 3367 1872
Size: 1495 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2410 2513 2567 4144 5045 5307
Size: 197 926 467 103 54 1577 901 262
Cuts at: 5307 820
Size: 1553
Fragments arranged by size:
1577 1553 926 901 467 262 197 103
54
SnaBI TAC'GTA
Cuts at: 493 3831 493
Size: 3338 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6040
SphI G_CATG'C
Cuts at: 1132 2379 3398 1132
Size: 1247 1019 3774
Fragments arranged by size:
3774 1247 1019
SspI AAT'ATT
Cuts at: 5 52 3964 5
Size: 47 3912 2081
Fragments arranged by size:
3912 2081 47
StuI AGG'CCT
Cuts at: 1517 2460 2700 2807 1517
Size: 943 240 107 4750
Fragments arranged by size:
4750 943 240 107
StyI C'CwwG_G
Cuts at: 513 1065 2004 2988 3009 3479 513
Size: 552 939 984 21 470 3074
Fragments arranged by size:
3074 984 939 552 470 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2029 2086 2981 3130 4067 4084 4237
Size: 1255 449 57 895 149 937 17 153
Cuts at: 4237 4422 4867 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 937 895 449 445 185 153
149 57 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4286 5998 96
Size: 274 80 33 51 1443 2309 1712 138
Fragments arranged by size:
2309 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2776 2808 6000 6032 2776
Size: 32 3192 32 2784
Fragments arranged by size:
3192 2784 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6040
XbaI T'CTAG_A
Cuts at: 3376 3376
Size: 6040
XhoI C'TCGA_G
Cuts at: 1943 2513 1943
Size: 570 5470
XmaI C'CCGG_G
Cuts at: 1870 3365 1870
Size: 1495 4545
XmnI GAAnn'nnTTC
Cuts at: 4169 4169
Size: 6040
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3848 276
Size: 53 83 186 936 2314 2468
Fragments arranged by size:
2468 2314 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstZ17I EcoICRI EcoRV FalI FspAI
KasI NarI NruI NsiI PacI PfoI PspOMI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI