Restriction map for
pFN21AE9517
(Circular) (Six-base) MAPSORT of: pFN21AE9517.seq Check: 2566 from: 1 to: 6427
pFN21AE9517 6427 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4237 278
Size: 53 83 186 936 2701 2468
Fragments arranged by size:
2701 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3210 3297 3770 1937
Size: 1273 87 473 4594
Fragments arranged by size:
4594 1273 473 87
Acc65I G'GTAC_C
Cuts at: 3748 3748
Size: 6427
AclI AA'CG_TT
Cuts at: 1563 4554 4927 1563
Size: 2991 373 3063
Fragments arranged by size:
3063 2991 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2833 3409 4490 5874 6142 1632
Size: 1201 576 1081 1384 268 1917
Fragments arranged by size:
1917 1384 1201 1081 576 268
AfeI AGC'GCT
Cuts at: 2957 2957
Size: 6427
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6230
AflIII A'CryG_T
Cuts at: 1203 2496 5326 6303 1203
Size: 1293 2830 977 1327
Fragments arranged by size:
2830 1327 1293 977
AgeI A'CCGG_T
Cuts at: 5258 5258
Size: 6427
AhdI GACnn_n'nnGTC
Cuts at: 5156 5156
Size: 6427
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6427
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2147 3680 1184
Size: 963 1533 3931
Fragments arranged by size:
3931 1533 963
AlwNI CAG_nnn'CTG
Cuts at: 4170 5742 4170
Size: 1572 4855
ApaI G_GGCC'C
Cuts at: 2519 2519
Size: 6427
ApaLI G'TGCA_C
Cuts at: 2173 2252 2332 2462 4484 5640 2173
Size: 79 80 130 2022 1156 2960
Fragments arranged by size:
2960 2022 1156 130 80 79
ApoI r'AATT_y
Cuts at: 1493 3736 3962 4114 6223 1493
Size: 2243 226 152 2109 1697
Fragments arranged by size:
2243 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4981 160
Size: 872 3949 1606
Fragments arranged by size:
3949 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6427
AvaI C'yCGr_G
Cuts at: 1870 1943 2246 2432 2587 3752 1870
Size: 73 303 186 155 1165 4545
Fragments arranged by size:
4545 1165 303 186 155 73
BamHI G'GATC_C
Cuts at: 3757 4124 3757
Size: 367 6060
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2090 2650 2696 2993 3283
Size: 325 217 627 303 560 46 297 290
Cuts at: 3283 3377 3675 3748 5203 618
Size: 94 298 73 1455 1842
Fragments arranged by size:
1842 1455 627 560 325 303 298 297
290 217 94 73 46
BanII G_rGCy'C
Cuts at: 1391 2519 3578 3746 1391
Size: 1128 1059 168 4072
Fragments arranged by size:
4072 1128 1059 168
BbeI G_GCGC'C
Cuts at: 2094 2700 3287 3679 2094
Size: 606 587 392 4842
Fragments arranged by size:
4842 606 587 392
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3415 3583 928
Size: 971 1516 168 3772
Fragments arranged by size:
3772 1516 971 168
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2164 2198 2323 2357 4618
Size: 34 50 34 143 34 125 34 2261
Cuts at: 4618 4652 1903
Size: 34 3678
Fragments arranged by size:
3678 2261 143 125 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3362 4319 5529 3362
Size: 957 1210 4260
Fragments arranged by size:
4260 1210 957
BclI T'GATC_A
Cuts at: 1548 3597 1548
Size: 2049 4378
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2437 2523 3128
Size: 107 122 71 631 605 765 86 605
Cuts at: 3128 3161 3555 5038 136
Size: 33 394 1483 1525
Fragments arranged by size:
1525 1483 765 631 605 605 394 122
107 86 71 33
BglII A'GATC_T
Cuts at: 2478 6422 2478
Size: 3944 2483
BlpI GC'TnA_GC
Cuts at: 3224 3844 3224
Size: 620 5807
Bme1580I G_kGCm'C
Cuts at: 1790 2056 2177 2256 2336 2466 2519 2655 2996
Size: 266 121 79 80 130 53 136 341
Cuts at: 2996 3171 3447 3546 4488 5644 1790
Size: 175 276 99 942 1156 2573
Fragments arranged by size:
2573 1156 942 341 276 266 175 136
130 121 99 80 79 53
BmgBI CAC'GTC
Cuts at: 1318 2499 3315 3525 1318
Size: 1181 816 210 4220
Fragments arranged by size:
4220 1181 816 210
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3508 4158 5116
Size: 416 53 83 399 24 2091 650 958
Cuts at: 5116 442
Size: 1753
Fragments arranged by size:
2091 1753 958 650 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6427
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2977 3151 2977
Size: 174 6253
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3235 3416 5087 1239
Size: 1996 181 1671 2579
Fragments arranged by size:
2579 1996 1671 181
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2630 4552 5417 5715 2630
Size: 1922 865 298 3342
Fragments arranged by size:
3342 1922 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5090 882
Size: 617 3591 2219
Fragments arranged by size:
3591 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2914 4218 493
Size: 711 1710 1304 2702
Fragments arranged by size:
2702 1710 1304 711
BsaBI GATnn'nnATC
Cuts at: 6421 6421
Size: 6427
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2091 2611 2697 3284
Size: 53 83 186 936 558 520 86 587
Cuts at: 3284 3420 3676 4234 4616 5251 275
Size: 136 256 558 382 635 1451
Fragments arranged by size:
1451 936 635 587 558 558 520 382
256 186 136 86 83 53
BsaWI w'CCGG_w
Cuts at: 4860 5258 5532 5679 6090 4860
Size: 398 274 147 411 5197
Fragments arranged by size:
5197 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6202
Fragments arranged by size:
6202 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2759 2762 1186
Size: 1573 3 4851
Fragments arranged by size:
4851 1573 3
BseYI C'CCAG_C
Cuts at: 2071 2215 5630 6126 2071
Size: 144 3415 496 2372
Fragments arranged by size:
3415 2372 496 144
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2300 2995 1163
Size: 573 564 695 4595
Fragments arranged by size:
4595 695 573 564
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2025 3099 3877 4638 5666 1540
Size: 275 186 24 1074 778 761 1028 2301
Fragments arranged by size:
2301 1074 1028 778 761 275 186 24
BsiHKAI G_wGCw'C
Cuts at: 2177 2256 2336 2343 2466 3033 3435 3498 4488
Size: 79 80 7 123 567 402 63 990
Cuts at: 4488 4573 5644 2177
Size: 85 1071 2960
Fragments arranged by size:
2960 1071 990 567 402 123 85 80
79 63 7
BsmI GAATG_Cn'
Cuts at: 3938 4031 3938
Size: 93 6334
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2056 2177 2256 2336 2343 2466 2519
Size: 399 266 121 79 80 7 123 53
Cuts at: 2519 2655 2996 3033 3171 3435 3447 3498 3546
Size: 136 341 37 138 264 12 51 48
Cuts at: 3546 3578 3746 4488 4573 5644 1391
Size: 32 168 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 341 266 264 168
138 136 123 121 85 80 79 53
51 48 37 32 12 7
BspEI T'CCGG_A
Cuts at: 6090 6090
Size: 6427
BspHI T'CATG_A
Cuts at: 2406 3363 3594 4316 6046 2406
Size: 957 231 722 1730 2787
Fragments arranged by size:
2787 1730 957 722 231
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2220 2565 2574 3228 3782 844
Size: 1376 345 9 654 554 3489
Fragments arranged by size:
3489 1376 654 554 345 9
BsrBI CCG'CTC
Cuts at: 1394 1823 2027 2437 2863 3228 4314 1394
Size: 429 204 410 426 365 1086 3507
Fragments arranged by size:
3507 1086 429 426 410 365 204
BsrDI GCAATG_nn'
Cuts at: 65 1248 4922 5096 65
Size: 1183 3674 174 1396
Fragments arranged by size:
3674 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2918 3098 3329 5071 5258 1700
Size: 110 1108 180 231 1742 187 2869
Fragments arranged by size:
2869 1742 1108 231 187 180 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6427
BssHII G'CGCG_C
Cuts at: 1925 2380 1925
Size: 455 5972
BssSI C'ACGA_G
Cuts at: 3116 4487 5499 3116
Size: 1371 1012 4044
Fragments arranged by size:
4044 1371 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6427
BstBI TT'CG_AA
Cuts at: 5231 5231
Size: 6427
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6427
BstXI CCAn_nnnn'nTGG
Cuts at: 2215 3460 2215
Size: 1245 5182
BstYI r'GATC_y
Cuts at: 1972 2478 3645 3757 4124 4509 4526 5978 6064
Size: 506 1167 112 367 385 17 1452 86
Cuts at: 6064 6076 6422 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1167 506 385 367 346 112
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2085 2110 2140 2904 3198 513
Size: 552 1020 25 30 764 294 3742
Fragments arranged by size:
3742 1020 764 552 294 30 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2167 487
Size: 683 56 941 4747
Fragments arranged by size:
4747 941 683 56
BtsI GCAGTG_nn'
Cuts at: 2288 2532 3943 4737 4757 2288
Size: 244 1411 794 20 3958
Fragments arranged by size:
3958 1411 794 244 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6427
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6392
DraI TTT'AAA
Cuts at: 3731 3731
Size: 6427
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3587 3667 5434 809
Size: 507 2271 80 1767 1802
Fragments arranged by size:
2271 1802 1767 507 80
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2022 2901 2916
Size: 54 1425 50 275 18 192 879 15
Cuts at: 2916 3048 3096 3153 3195 3327 3556 3874 4763
Size: 132 48 57 42 132 229 318 889
Cuts at: 4763 6392 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 879 318 275 229 192
132 132 57 54 50 48 43 42
18 15
EagI C'GGCC_G
Cuts at: 1537 1812 2022 3096 3874 1537
Size: 275 210 1074 778 4090
Fragments arranged by size:
4090 1074 778 275 210
EarI CTCTTCn'nnn_
Cuts at: 1353 3026 3308 3350 4357 1353
Size: 1673 282 42 1007 3423
Fragments arranged by size:
3423 1673 1007 282 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5014 5388 5534 387
Size: 1064 138 3425 374 146 1280
Fragments arranged by size:
3425 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2833 3235 3409 3416 4490 5087 5874
Size: 393 1201 402 174 7 1074 597 787
Cuts at: 5874 6142 1239
Size: 268 1524
Fragments arranged by size:
1524 1201 1074 787 597 402 393 268
174 7
EcoNI CCTnn'n_nnAGG
Cuts at: 2930 2930
Size: 6427
EcoO109I rG'GnC_Cy
Cuts at: 2011 2515 2592 3217 2011
Size: 504 77 625 5221
Fragments arranged by size:
5221 625 504 77
EcoRI G'AATT_C
Cuts at: 3736 4114 3736
Size: 378 6049
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2437 2437
Size: 6427
FspI TGC'GCA
Cuts at: 3413 4933 3413
Size: 1520 4907
FspAI rTGC'GCAy
Cuts at: 3413 3413
Size: 6427
HaeII r_GCGC'y
Cuts at: 1678 2069 2094 2405 2700 2758 2848 2959 3287
Size: 391 25 311 295 58 90 111 328
Cuts at: 3287 3679 5574 1678
Size: 392 1895 2531
Fragments arranged by size:
2531 1895 392 391 328 311 295 111
90 58 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5068 5100 5142 5174 1373
Size: 32 135 32 3496 32 42 32 2626
Fragments arranged by size:
3496 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3771 4017 669
Size: 988 281 1833 246 3079
Fragments arranged by size:
3079 1833 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6427
HpaI GTT'AAC
Cuts at: 4017 4017
Size: 6427
KasI G'GCGC_C
Cuts at: 2090 2696 3283 3675 2090
Size: 606 587 392 4842
Fragments arranged by size:
4842 606 587 392
KpnI G_GTAC'C
Cuts at: 3752 3752
Size: 6427
MfeI C'AATT_G
Cuts at: 4026 4026
Size: 6427
MluI A'CGCG_T
Cuts at: 6303 6303
Size: 6427
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2481 5541 5725 1305
Size: 1176 3060 184 2007
Fragments arranged by size:
3060 2007 1176 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3050 3197 10
Size: 54 1425 343 1218 147 3240
Fragments arranged by size:
3240 1425 1218 343 147 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2129 3362 4385 4744 4903 518
Size: 648 963 1233 1023 359 159 2042
Fragments arranged by size:
2042 1233 1023 963 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2087 2170 2269 3843 4520 5668 5913 1606
Size: 481 83 99 1574 677 1148 245 2120
Fragments arranged by size:
2120 1574 1148 677 481 245 99 83
NaeI GCC'GGC
Cuts at: 2920 3100 3331 2920
Size: 180 231 6016
Fragments arranged by size:
6016 231 180
NarI GG'CG_CC
Cuts at: 2091 2697 3284 3676 2091
Size: 606 587 392 4842
Fragments arranged by size:
4842 606 587 392
NcoI C'CATG_G
Cuts at: 513 1065 3198 513
Size: 552 2133 3742
Fragments arranged by size:
3742 2133 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6427
NgoMIV G'CCGG_C
Cuts at: 2918 3098 3329 2918
Size: 180 231 6016
Fragments arranged by size:
6016 231 180
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6427
NotI GC'GGCC_GC
Cuts at: 2022 3874 2022
Size: 1852 4575
NspI r_CATG'y
Cuts at: 1132 1229 2842 3785 5330 1132
Size: 97 1613 943 1545 2229
Fragments arranged by size:
2229 1613 1545 943 97
PasI CC'CwG_GG
Cuts at: 1348 2041 1348
Size: 693 5734
PciI A'CATG_T
Cuts at: 5326 5326
Size: 6427
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3063 1108
Size: 1955 4472
PmeI GTTT'AAAC
Cuts at: 3731 3731
Size: 6427
PmlI CAC'GTG
Cuts at: 2914 2914
Size: 6427
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4496 4528 6009 6041 4496
Size: 32 1481 32 4882
Fragments arranged by size:
4882 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2011 3217 2011
Size: 1206 5221
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6427
PsiI TTA'TAA
Cuts at: 3997 3997
Size: 6427
PspOMI G'GGCC_C
Cuts at: 2515 2515
Size: 6427
PspXI vC'TCGA_Gb
Cuts at: 2246 2246
Size: 6427
PstI C_TGCA'G
Cuts at: 830 3522 3669 3779 830
Size: 2692 147 110 3478
Fragments arranged by size:
3478 2692 147 110
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6427
SacII CC_GC'GG
Cuts at: 2088 2088
Size: 6427
SalI G'TCGA_C
Cuts at: 1936 3769 1936
Size: 1833 4594
SanDI GG'GwC_CC
Cuts at: 2011 2011
Size: 6427
SbfI CC_TGCA'GG
Cuts at: 3779 3779
Size: 6427
ScaI AGT'ACT
Cuts at: 3143 4675 6387 3143
Size: 1532 1712 3183
Fragments arranged by size:
3183 1712 1532
SexAI A'CCwGG_T
Cuts at: 2829 2829
Size: 6427
SfcI C'TryA_G
Cuts at: 826 1045 3518 3665 3775 4910 5591 5782 826
Size: 219 2473 147 110 1135 681 191 1471
Fragments arranged by size:
2473 1471 1135 681 219 191 147 110
SfiI GGCCn_nnn'nGGCC
Cuts at: 3128 3161 3128
Size: 33 6394
SfoI GGC'GCC
Cuts at: 2092 2698 3285 3677 2092
Size: 606 587 392 4842
Fragments arranged by size:
4842 606 587 392
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6427
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6427
SmaI CCC'GGG
Cuts at: 1872 2589 3754 1872
Size: 717 1165 4545
Fragments arranged by size:
4545 1165 717
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2246 2645 4531 5432 5694 820
Size: 197 926 303 399 1886 901 262 1553
Fragments arranged by size:
1886 1553 926 901 399 303 262 197
SnaBI TAC'GTA
Cuts at: 493 4218 493
Size: 3725 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6427
SphI G_CATG'C
Cuts at: 1132 3785 1132
Size: 2653 3774
SspI AAT'ATT
Cuts at: 5 52 4351 5
Size: 47 4299 2081
Fragments arranged by size:
4299 2081 47
StuI AGG'CCT
Cuts at: 1517 3485 1517
Size: 1968 4459
StyI C'CwwG_G
Cuts at: 513 1065 3198 3866 513
Size: 552 2133 668 3074
Fragments arranged by size:
3074 2133 668 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2669 2761 3401 4454 4471 4624 4809
Size: 1255 1089 92 640 1053 17 153 185
Cuts at: 4809 5254 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1089 1053 640 445 185 153
92 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2733 3141 4673
Size: 274 80 33 51 1443 756 408 1532
Cuts at: 4673 6385 96
Size: 1712 138
Fragments arranged by size:
1712 1532 1443 756 408 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6387 6419 6387
Size: 32 6395
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6427
XbaI T'CTAG_A
Cuts at: 2929 3763 2929
Size: 834 5593
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3022 3190 3457 3022
Size: 168 267 5992
Fragments arranged by size:
5992 267 168
XhoI C'TCGA_G
Cuts at: 1943 2246 1943
Size: 303 6124
XmaI C'CCGG_G
Cuts at: 1870 2587 3752 1870
Size: 717 1165 4545
Fragments arranged by size:
4545 1165 717
XmnI GAAnn'nnTTC
Cuts at: 2414 4556 2414
Size: 2142 4285
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4235 276
Size: 53 83 186 936 2701 2468
Fragments arranged by size:
2701 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI SacII SalI SanDI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI
NruI NsiI PacI PfoI PsrI PvuII RsrII SacI
SapI SrfI SwaI