Restriction map for
pFN21AE9516
(Circular) (Six-base) MAPSORT of: pFN21AE9516.seq Check: 7362 from: 1 to: 6931
pFN21AE9516 6931 bp
With 182 enzymes: SgfI *
July 6, 2012 17:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3305 3483 3305
Size: 178 6753
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4741 278
Size: 53 83 186 936 3205 2468
Fragments arranged by size:
3205 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4274 1937
Size: 2337 4594
Acc65I G'GTAC_C
Cuts at: 4252 4252
Size: 6931
AclI AA'CG_TT
Cuts at: 1563 5058 5431 1563
Size: 3495 373 3063
Fragments arranged by size:
3495 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4994 6378 6646 1632
Size: 3362 1384 268 1917
Fragments arranged by size:
3362 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6734
AflIII A'CryG_T
Cuts at: 1203 5830 6807 1203
Size: 4627 977 1327
Fragments arranged by size:
4627 1327 977
AgeI A'CCGG_T
Cuts at: 5762 5762
Size: 6931
AhdI GACnn_n'nnGTC
Cuts at: 5660 5660
Size: 6931
AleI CACnn'nnGTG
Cuts at: 1166 3380 1166
Size: 2214 4717
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6931
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2966 2998 2966
Size: 32 6899
AlwNI CAG_nnn'CTG
Cuts at: 2079 2578 3202 3271 3673 3774 4674 6246 2079
Size: 499 624 69 402 101 900 1572 2764
Fragments arranged by size:
2764 1572 900 624 499 402 101 69
ApaI G_GGCC'C
Cuts at: 4207 4207
Size: 6931
ApaLI G'TGCA_C
Cuts at: 4988 6144 4988
Size: 1156 5775
ApoI r'AATT_y
Cuts at: 1493 2237 4160 4240 4466 4618 6727 1493
Size: 744 1923 80 226 152 2109 1697
Fragments arranged by size:
2109 1923 1697 744 226 152 80
AseI AT'TA_AT
Cuts at: 160 1032 5485 160
Size: 872 4453 1606
Fragments arranged by size:
4453 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6931
AvaI C'yCGr_G
Cuts at: 1870 1943 2287 4200 4256 1870
Size: 73 344 1913 56 4545
Fragments arranged by size:
4545 1913 344 73 56
BamHI G'GATC_C
Cuts at: 2742 2902 4261 4628 2742
Size: 160 1359 367 5045
Fragments arranged by size:
5045 1359 367 160
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2091 2680 2694 4061 4252
Size: 325 217 627 304 589 14 1367 191
Cuts at: 4252 5707 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1367 627 589 325 304 217
191 14
BanII G_rGCy'C
Cuts at: 1391 3458 3506 3636 4207 4250 1391
Size: 2067 48 130 571 43 4072
Fragments arranged by size:
4072 2067 571 130 48 43
BbeI G_GCGC'C
Cuts at: 2684 4065 2684
Size: 1381 5550
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5960
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2199 2233 5122 5156 1903
Size: 34 262 34 2889 34 3678
Fragments arranged by size:
3678 2889 262 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4823 6033 4823
Size: 1210 5721
BclI T'GATC_A
Cuts at: 1548 3372 1548
Size: 1824 5107
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2442 3624 3699
Size: 107 122 71 631 605 770 1182 75
Cuts at: 3699 4059 4211 5542 136
Size: 360 152 1331 1525
Fragments arranged by size:
1525 1331 1182 770 631 605 360 152
122 107 75 71
BglII A'GATC_T
Cuts at: 6926 6926
Size: 6931
BlpI GC'TnA_GC
Cuts at: 2602 4348 2602
Size: 1746 5185
Bme1580I G_kGCm'C
Cuts at: 1790 2086 2096 3246 3714 4207 4992 6148 1790
Size: 296 10 1150 468 493 785 1156 2573
Fragments arranged by size:
2573 1156 1150 785 493 468 296 10
BmgBI CAC'GTC
Cuts at: 1318 3178 3349 4176 1318
Size: 1860 171 827 4073
Fragments arranged by size:
4073 1860 827 171
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3256 4662 5620
Size: 416 53 83 399 24 1839 1406 958
Cuts at: 5620 442
Size: 1753
Fragments arranged by size:
1839 1753 1406 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6931
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2383 2383
Size: 6931
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2056 2550 2682 2913 3023 3650 3826 5591
Size: 817 494 132 231 110 627 176 1765
Cuts at: 5591 1239
Size: 2579
Fragments arranged by size:
2579 1765 817 627 494 231 176 132
110
Bpu10I CC'TnA_GC
Cuts at: 3314 3314
Size: 6931
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3152 5056 5921 6219 3152
Size: 1904 865 298 3864
Fragments arranged by size:
3864 1904 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3153 5594 882
Size: 617 1654 2441 2219
Fragments arranged by size:
2441 2219 1654 617
BsaAI yAC'GTr
Cuts at: 493 1204 4722 493
Size: 711 3518 2702
Fragments arranged by size:
3518 2702 711
BsaBI GATnn'nnATC
Cuts at: 6925 6925
Size: 6931
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2681 3046 3729 4062
Size: 53 83 186 936 1148 365 683 333
Cuts at: 4062 4738 5120 5755 275
Size: 676 382 635 1451
Fragments arranged by size:
1451 1148 936 683 676 635 382 365
333 186 83 53
BsaWI w'CCGG_w
Cuts at: 4116 5364 5762 6036 6183 6594 4116
Size: 1248 398 274 147 411 4453
Fragments arranged by size:
4453 1248 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2001 2031 1178
Size: 30 165 30 598 30 6078
Fragments arranged by size:
6078 598 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2095 3020 1186
Size: 909 925 5097
Fragments arranged by size:
5097 925 909
BseYI C'CCAG_C
Cuts at: 2709 3271 4139 6134 6630 2709
Size: 562 868 1995 496 3010
Fragments arranged by size:
3010 1995 868 562 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2302 2383 3461 3566 3961 4064 1163
Size: 573 566 81 1078 105 395 103 4030
Fragments arranged by size:
4030 1078 573 566 395 105 103 81
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3763 4012 4381 5142 6170 1540
Size: 275 186 1762 249 369 761 1028 2301
Fragments arranged by size:
2301 1762 1028 761 369 275 249 186
BsiHKAI G_wGCw'C
Cuts at: 3636 4992 5077 6148 3636
Size: 1356 85 1071 4419
Fragments arranged by size:
4419 1356 1071 85
BsmI GAATG_Cn'
Cuts at: 2027 2980 4442 4535 2027
Size: 953 1462 93 4423
Fragments arranged by size:
4423 1462 953 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2658 3736 2658
Size: 1078 5853
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2086 2096 3246 3458 3506 3636 3714
Size: 399 296 10 1150 212 48 130 78
Cuts at: 3714 4207 4250 4992 5077 6148 1391
Size: 493 43 742 85 1071 2174
Fragments arranged by size:
2174 1150 1071 742 493 399 296 212
130 85 78 48 43 10
BspEI T'CCGG_A
Cuts at: 6594 6594
Size: 6931
BspHI T'CATG_A
Cuts at: 2736 4820 6550 2736
Size: 2084 1730 3117
Fragments arranged by size:
3117 2084 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2985 3305 3483 4083 4286 844
Size: 2141 320 178 600 203 3489
Fragments arranged by size:
3489 2141 600 320 203 178
BsrBI CCG'CTC
Cuts at: 1394 1823 2824 4818 1394
Size: 429 1001 1994 3507
Fragments arranged by size:
3507 1994 1001 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2951 3850 5426 5600 65
Size: 1183 1703 899 1576 174 1396
Fragments arranged by size:
1703 1576 1396 1183 899 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3521 5575 5762 1700
Size: 110 1711 2054 187 2869
Fragments arranged by size:
2869 2054 1711 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6931
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6931
BssSI C'ACGA_G
Cuts at: 2107 4991 6003 2107
Size: 2884 1012 3035
Fragments arranged by size:
3035 2884 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3202 3297 4087 794
Size: 2408 95 790 3638
Fragments arranged by size:
3638 2408 790 95
BstBI TT'CG_AA
Cuts at: 2792 5735 2792
Size: 2943 3988
BstEII G'GTnAC_C
Cuts at: 1184 2611 1184
Size: 1427 5504
BstXI CCAn_nnnn'nTGG
Cuts at: 3517 3517
Size: 6931
BstYI r'GATC_y
Cuts at: 1972 2742 2902 4261 4628 5013 5030 6482 6568
Size: 770 160 1359 367 385 17 1452 86
Cuts at: 6568 6580 6926 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1359 770 385 367 346 160
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4208 4208
Size: 6931
BtgI C'CryG_G
Cuts at: 513 1065 2004 2412 2916 3028 3191 513
Size: 552 939 408 504 112 163 4253
Fragments arranged by size:
4253 939 552 504 408 163 112
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3937 487
Size: 683 56 2711 3481
Fragments arranged by size:
3481 2711 683 56
BtsI GCAGTG_nn'
Cuts at: 4081 4447 5241 5261 4081
Size: 366 794 20 5751
Fragments arranged by size:
5751 794 366 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6931
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6896
DraI TTT'AAA
Cuts at: 4235 4235
Size: 6931
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2837 5938 809
Size: 507 1521 3101 1802
Fragments arranged by size:
3101 1802 1521 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2748 2958 3519
Size: 54 1425 50 275 18 918 210 561
Cuts at: 3519 3541 3570 3597 3760 3948 4186 4378 5267
Size: 22 29 27 163 188 238 192 889
Cuts at: 5267 6896 8
Size: 1629 43
Fragments arranged by size:
1629 1425 918 889 561 275 238 210
192 188 163 54 50 43 29 27
22 18
EagI C'GGCC_G
Cuts at: 1537 1812 3760 4378 1537
Size: 275 1948 618 4090
Fragments arranged by size:
4090 1948 618 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3151 3215 3407 3500 3968 4861 1353
Size: 1798 64 192 93 468 893 3423
Fragments arranged by size:
3423 1798 893 468 192 93 64
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5518 5892 6038 387
Size: 1064 138 3929 374 146 1280
Fragments arranged by size:
3929 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3634 3634
Size: 6931
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2056 2550 2682 2913 3023 3650 3826
Size: 393 424 494 132 231 110 627 176
Cuts at: 3826 4994 5591 6378 6646 1239
Size: 1168 597 787 268 1524
Fragments arranged by size:
1524 1168 787 627 597 494 424 393
268 231 176 132 110
EcoO109I rG'GnC_Cy
Cuts at: 2575 2674 2768 2909 3670 3818 4204 2575
Size: 99 94 141 761 148 386 5302
Fragments arranged by size:
5302 761 386 148 141 99 94
EcoRI G'AATT_C
Cuts at: 4160 4240 4618 4160
Size: 80 378 6473
Fragments arranged by size:
6473 378 80
EcoRV GAT'ATC
Cuts at: 2734 2734
Size: 6931
FspI TGC'GCA
Cuts at: 5437 5437
Size: 6931
HaeII r_GCGC'y
Cuts at: 1678 2684 3395 3938 4065 6078 1678
Size: 1006 711 543 127 2013 2531
Fragments arranged by size:
2531 2013 1006 711 543 127
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2001 2033 2448 2480 2641
Size: 32 135 32 429 32 415 32 161
Cuts at: 2641 2673 5572 5604 5646 5678 1373
Size: 32 2899 32 42 32 2626
Fragments arranged by size:
2899 2626 429 415 161 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3061 4275 4521 669
Size: 988 281 1123 1214 246 3079
Fragments arranged by size:
3079 1214 1123 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6931
HpaI GTT'AAC
Cuts at: 4521 4521
Size: 6931
KasI G'GCGC_C
Cuts at: 2680 4061 2680
Size: 1381 5550
KpnI G_GTAC'C
Cuts at: 4256 4256
Size: 6931
MfeI C'AATT_G
Cuts at: 4530 4530
Size: 6931
MluI A'CGCG_T
Cuts at: 6807 6807
Size: 6931
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3061 3762 6045 6229 1305
Size: 1756 701 2283 184 2007
Fragments arranged by size:
2283 2007 1756 701 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2750 2960 4188 10
Size: 54 1425 343 918 210 1228 2753
Fragments arranged by size:
2753 1425 1228 918 343 210 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2382 3380 3857 4889 5248 5407 518
Size: 648 1216 998 477 1032 359 159 2042
Fragments arranged by size:
2042 1216 1032 998 648 477 359 159
MspA1I CmG'CkG
Cuts at: 1606 2427 2433 2713 2891 4347 5024 6172 6417
Size: 821 6 280 178 1456 677 1148 245
Cuts at: 6417 1606
Size: 2120
Fragments arranged by size:
2120 1456 1148 821 677 280 245 178
6
NaeI GCC'GGC
Cuts at: 3523 3523
Size: 6931
NarI GG'CG_CC
Cuts at: 2681 4062 2681
Size: 1381 5550
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5440
Fragments arranged by size:
5440 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6931
NgoMIV G'CCGG_C
Cuts at: 3521 3521
Size: 6931
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6931
NotI GC'GGCC_GC
Cuts at: 4378 4378
Size: 6931
NspI r_CATG'y
Cuts at: 1132 1229 4289 5834 1132
Size: 97 3060 1545 2229
Fragments arranged by size:
3060 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6931
PciI A'CATG_T
Cuts at: 5830 5830
Size: 6931
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2578 3271 1108
Size: 1470 693 4768
Fragments arranged by size:
4768 1470 693
PfoI T'CCnGG_A
Cuts at: 2571 2659 2817 2571
Size: 88 158 6685
Fragments arranged by size:
6685 158 88
PmeI GTTT'AAAC
Cuts at: 4235 4235
Size: 6931
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5000 5032 6513 6545 5000
Size: 32 1481 32 5386
Fragments arranged by size:
5386 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2575 3818 2575
Size: 1243 5688
PshAI GACnn'nnGTC
Cuts at: 1613 3866 1613
Size: 2253 4678
PsiI TTA'TAA
Cuts at: 4501 4501
Size: 6931
PspOMI G'GGCC_C
Cuts at: 4203 4203
Size: 6931
PstI C_TGCA'G
Cuts at: 830 4059 4283 830
Size: 3229 224 3478
Fragments arranged by size:
3478 3229 224
PvuI CG_AT'CG
Cuts at: 2001 4012 2001
Size: 2011 4920
PvuII CAG'CTG
Cuts at: 2427 2713 2891 2427
Size: 286 178 6467
Fragments arranged by size:
6467 286 178
SacI G_AGCT'C
Cuts at: 3636 3636
Size: 6931
SalI G'TCGA_C
Cuts at: 1936 4273 1936
Size: 2337 4594
SapI GCTCTTCn'nnn_
Cuts at: 3968 3968
Size: 6931
SbfI CC_TGCA'GG
Cuts at: 4059 4283 4059
Size: 224 6707
ScaI AGT'ACT
Cuts at: 4154 5179 6891 4154
Size: 1025 1712 4194
Fragments arranged by size:
4194 1712 1025
SfcI C'TryA_G
Cuts at: 826 1045 3745 4055 4279 5414 6095 6286 826
Size: 219 2700 310 224 1135 681 191 1471
Fragments arranged by size:
2700 1471 1135 681 310 224 219 191
SfoI GGC'GCC
Cuts at: 2682 4063 2682
Size: 1381 5550
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6931
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6931
SmaI CCC'GGG
Cuts at: 1872 2289 4202 4258 1872
Size: 417 1913 56 4545
Fragments arranged by size:
4545 1913 417 56
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3131 5035 5936 6198 820
Size: 197 926 1188 1904 901 262 1553
Fragments arranged by size:
1904 1553 1188 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4722 493
Size: 4229 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6931
SphI G_CATG'C
Cuts at: 1132 4289 1132
Size: 3157 3774
SspI AAT'ATT
Cuts at: 5 52 4855 5
Size: 47 4803 2081
Fragments arranged by size:
4803 2081 47
StuI AGG'CCT
Cuts at: 1517 3627 1517
Size: 2110 4821
StyI C'CwwG_G
Cuts at: 513 1065 2004 3090 3171 3661 4370 513
Size: 552 939 1086 81 490 709 3074
Fragments arranged by size:
3074 1086 939 709 552 490 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2723 4958 4975 5128 5313 5758 325
Size: 1255 1143 2235 17 153 185 445 1498
Fragments arranged by size:
2235 1498 1255 1143 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4152 5177 6889
Size: 274 80 33 51 1443 2175 1025 1712
Cuts at: 6889 96
Size: 138
Fragments arranged by size:
2175 1712 1443 1025 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3119 3151 6891 6923 3119
Size: 32 3740 32 3127
Fragments arranged by size:
3740 3127 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2486 2567 1316
Size: 1170 81 5680
Fragments arranged by size:
5680 1170 81
XbaI T'CTAG_A
Cuts at: 4267 4267
Size: 6931
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3385 3514 3385
Size: 129 6802
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6931
XmaI C'CCGG_G
Cuts at: 1870 2287 4200 4256 1870
Size: 417 1913 56 4545
Fragments arranged by size:
4545 1913 417 56
XmnI GAAnn'nnTTC
Cuts at: 5060 5060
Size: 6931
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4739 276
Size: 53 83 186 936 3205 2468
Fragments arranged by size:
3205 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I
DraIII EcoNI FalI FseI FspAI NruI NsiI PacI
PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI
SrfI SwaI