Restriction map for
pFN21AE9515
(Circular) (Six-base) MAPSORT of: pFN21AE9515.seq Check: 6321 from: 1 to: 5815
pFN21AE9515 5815 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3625 278
Size: 53 83 186 936 2089 2468
Fragments arranged by size:
2468 2089 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3158 1937
Size: 1221 4594
Acc65I G'GTAC_C
Cuts at: 3136 3136
Size: 5815
AclI AA'CG_TT
Cuts at: 1563 3942 4315 1563
Size: 2379 373 3063
Fragments arranged by size:
3063 2379 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2201 3878 5262 5530 1632
Size: 569 1677 1384 268 1917
Fragments arranged by size:
1917 1677 1384 569 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5618
AflIII A'CryG_T
Cuts at: 1203 4714 5691 1203
Size: 3511 977 1327
Fragments arranged by size:
3511 1327 977
AgeI A'CCGG_T
Cuts at: 4646 4646
Size: 5815
AhdI GACnn_n'nnGTC
Cuts at: 4544 4544
Size: 5815
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5815
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2258 2537 1184
Size: 1074 279 4462
Fragments arranged by size:
4462 1074 279
AlwNI CAG_nnn'CTG
Cuts at: 2161 3558 5130 2161
Size: 1397 1572 2846
Fragments arranged by size:
2846 1572 1397
ApaI G_GGCC'C
Cuts at: 2030 2030
Size: 5815
ApaLI G'TGCA_C
Cuts at: 2332 2495 2591 3872 5028 2332
Size: 163 96 1281 1156 3119
Fragments arranged by size:
3119 1281 1156 163 96
ApoI r'AATT_y
Cuts at: 1493 3124 3350 3502 5611 1493
Size: 1631 226 152 2109 1697
Fragments arranged by size:
2109 1697 1631 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4369 160
Size: 872 3337 1606
Fragments arranged by size:
3337 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5815
AvaI C'yCGr_G
Cuts at: 1870 1943 2022 3140 1870
Size: 73 79 1118 4545
Fragments arranged by size:
4545 1118 79 73
BamHI G'GATC_C
Cuts at: 3145 3512 3145
Size: 367 5448
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2773 2820 3136 4591 618
Size: 325 217 627 986 47 316 1455 1842
Fragments arranged by size:
1842 1455 986 627 325 316 217 47
BanII G_rGCy'C
Cuts at: 1391 2030 2613 2654 3134 1391
Size: 639 583 41 480 4072
Fragments arranged by size:
4072 639 583 480 41
BbeI G_GCGC'C
Cuts at: 2824 2824
Size: 5815
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2407 2884 928
Size: 971 508 477 3859
Fragments arranged by size:
3859 971 508 477
BbvCI CC'TCA_GC
Cuts at: 2504 2528 2504
Size: 24 5791
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2082 2116 4006 4040 1903
Size: 34 145 34 1890 34 3678
Fragments arranged by size:
3678 1890 145 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3707 4917 3707
Size: 1210 4605
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5815
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2555 4426 136
Size: 107 122 71 631 605 883 1871 1525
Fragments arranged by size:
1871 1525 883 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5810 5810
Size: 5815
BlpI GC'TnA_GC
Cuts at: 3232 3232
Size: 5815
Bme1580I G_kGCm'C
Cuts at: 1790 2030 2336 2499 2595 3876 5032 1790
Size: 240 306 163 96 1281 1156 2573
Fragments arranged by size:
2573 1281 1156 306 240 163 96
BmgBI CAC'GTC
Cuts at: 1318 2323 2626 2890 1318
Size: 1005 303 264 4243
Fragments arranged by size:
4243 1005 303 264
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2815 3546 4504
Size: 416 53 83 399 24 1398 731 958
Cuts at: 4504 442
Size: 1753
Fragments arranged by size:
1753 1398 958 731 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5815
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2121 4475 1239
Size: 882 2354 2579
Fragments arranged by size:
2579 2354 882
Bpu10I CC'TnA_GC
Cuts at: 2504 2528 2504
Size: 24 5791
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2597 3940 4805 5103 2597
Size: 1343 865 298 3309
Fragments arranged by size:
3309 1343 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4478 882
Size: 617 2979 2219
Fragments arranged by size:
2979 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2605 3606 493
Size: 711 1401 1001 2702
Fragments arranged by size:
2702 1401 1001 711
BsaBI GATnn'nnATC
Cuts at: 5809 5809
Size: 5815
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2821 3622 4004 4639
Size: 53 83 186 936 1288 801 382 635
Cuts at: 4639 275
Size: 1451
Fragments arranged by size:
1451 1288 936 801 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4248 4646 4920 5067 5478 4248
Size: 398 274 147 411 4585
Fragments arranged by size:
4585 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5590
Fragments arranged by size:
5590 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2654 3050 1186
Size: 1468 396 3951
Fragments arranged by size:
3951 1468 396
BseYI C'CCAG_C
Cuts at: 2382 5018 5514 2382
Size: 2636 496 2683
Fragments arranged by size:
2683 2636 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2261 2870 2972 3002 1163
Size: 573 525 609 102 30 3976
Fragments arranged by size:
3976 609 573 525 102 30
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3265 4026 5054 1540
Size: 275 186 1264 761 1028 2301
Fragments arranged by size:
2301 1264 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2328 2336 2499 2595 2613 3876 3961 5032 2328
Size: 8 163 96 18 1263 85 1071 3111
Fragments arranged by size:
3111 1263 1071 163 96 85 18 8
BsmI GAATG_Cn'
Cuts at: 3326 3419 3326
Size: 93 5722
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2328 2336 2499 2595 2613 2654
Size: 399 240 298 8 163 96 18 41
Cuts at: 2654 3134 3876 3961 5032 1391
Size: 480 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 480 399 298 240 163
96 85 41 18 8
BspEI T'CCGG_A
Cuts at: 5478 5478
Size: 5815
BspHI T'CATG_A
Cuts at: 3704 5434 3704
Size: 1730 4085
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2715 2907 3170 844
Size: 1871 192 263 3489
Fragments arranged by size:
3489 1871 263 192
BsrBI CCG'CTC
Cuts at: 1394 1823 2350 3702 1394
Size: 429 527 1352 3507
Fragments arranged by size:
3507 1352 527 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4310 4484 65
Size: 1183 3062 174 1396
Fragments arranged by size:
3062 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2062 2271 2428 2823 4459 4646 1700
Size: 110 252 209 157 395 1636 187 2869
Fragments arranged by size:
2869 1636 395 252 209 187 157 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5815
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5815
BssSI C'ACGA_G
Cuts at: 3875 4887 3875
Size: 1012 4803
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2170 2340 2626 2835 794
Size: 1376 170 286 209 3774
Fragments arranged by size:
3774 1376 286 209 170
BstBI TT'CG_AA
Cuts at: 4619 4619
Size: 5815
BstEII G'GTnAC_C
Cuts at: 1184 2431 2698 1184
Size: 1247 267 4301
Fragments arranged by size:
4301 1247 267
BstXI CCAn_nnnn'nTGG
Cuts at: 2382 2473 2382
Size: 91 5724
BstYI r'GATC_y
Cuts at: 1972 3145 3512 3897 3914 5366 5452 5464 5810
Size: 1173 367 385 17 1452 86 12 346
Cuts at: 5810 1972
Size: 1977
Fragments arranged by size:
1977 1452 1173 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2565 3082 513
Size: 552 939 561 517 3246
Fragments arranged by size:
3246 939 561 552 517
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2452 487
Size: 683 56 1226 3850
Fragments arranged by size:
3850 1226 683 56
BtsI GCAGTG_nn'
Cuts at: 2478 3331 4125 4145 2478
Size: 853 794 20 4148
Fragments arranged by size:
4148 853 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5815
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5780
DraI TTT'AAA
Cuts at: 3119 3119
Size: 5815
DraIII CAC_nnn'GTG
Cuts at: 2284 2626 2284
Size: 342 5473
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2483 4822 809
Size: 507 1167 2339 1802
Fragments arranged by size:
2339 1802 1167 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2547 3085 3262
Size: 54 1425 50 275 18 717 538 177
Cuts at: 3262 4151 5780 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 717 538 275 177 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3262 1537
Size: 275 1450 4090
Fragments arranged by size:
4090 1450 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3745 1353
Size: 2392 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4402 4776 4922 387
Size: 1064 138 2813 374 146 1280
Fragments arranged by size:
2813 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2611 2611
Size: 5815
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2121 2201 3878 4475 5262 5530 1239
Size: 393 489 80 1677 597 787 268 1524
Fragments arranged by size:
1677 1524 787 597 489 393 268 80
EcoNI CCTnn'n_nnAGG
Cuts at: 2636 2636
Size: 5815
EcoO109I rG'GnC_Cy
Cuts at: 2026 2027 2908 2026
Size: 1 881 4933
Fragments arranged by size:
4933 881 1
EcoRI G'AATT_C
Cuts at: 3124 3502 3124
Size: 378 5437
FspI TGC'GCA
Cuts at: 3044 4321 3044
Size: 1277 4538
HaeII r_GCGC'y
Cuts at: 1678 2535 2561 2579 2665 2671 2824 4962 1678
Size: 857 26 18 86 6 153 2138 2531
Fragments arranged by size:
2531 2138 857 153 86 26 18 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4456 4488 4530 4562 1373
Size: 32 135 32 2884 32 42 32 2626
Fragments arranged by size:
2884 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3159 3405 669
Size: 988 281 1221 246 3079
Fragments arranged by size:
3079 1221 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5815
HpaI GTT'AAC
Cuts at: 3405 3405
Size: 5815
KasI G'GCGC_C
Cuts at: 2820 2820
Size: 5815
KpnI G_GTAC'C
Cuts at: 3140 3140
Size: 5815
MfeI C'AATT_G
Cuts at: 3414 3414
Size: 5815
MluI A'CGCG_T
Cuts at: 5691 5691
Size: 5815
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2343 4929 5113 1305
Size: 1038 2586 184 2007
Fragments arranged by size:
2586 2007 1038 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2549 3087 10
Size: 54 1425 343 717 538 2738
Fragments arranged by size:
2738 1425 717 538 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2471 3773 4132 4291 518
Size: 648 1305 1302 359 159 2042
Fragments arranged by size:
2042 1305 1302 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2167 2179 2503 2567 2884 2956 3231 3908
Size: 561 12 324 64 317 72 275 677
Cuts at: 3908 5056 5301 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 561 324 317 275 245
72 64 12
NaeI GCC'GGC
Cuts at: 2064 2273 2825 2064
Size: 209 552 5054
Fragments arranged by size:
5054 552 209
NarI GG'CG_CC
Cuts at: 2821 2821
Size: 5815
NcoI C'CATG_G
Cuts at: 513 1065 2004 3082 513
Size: 552 939 1078 3246
Fragments arranged by size:
3246 1078 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5815
NgoMIV G'CCGG_C
Cuts at: 2062 2271 2823 2062
Size: 209 552 5054
Fragments arranged by size:
5054 552 209
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5815
NotI GC'GGCC_GC
Cuts at: 3262 3262
Size: 5815
NspI r_CATG'y
Cuts at: 1132 1229 3173 4718 1132
Size: 97 1944 1545 2229
Fragments arranged by size:
2229 1944 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2031 1348
Size: 683 5132
PciI A'CATG_T
Cuts at: 4714 4714
Size: 5815
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5815
PmeI GTTT'AAAC
Cuts at: 3119 3119
Size: 5815
PmlI CAC'GTG
Cuts at: 2605 2605
Size: 5815
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3884 3916 5397 5429 3884
Size: 32 1481 32 4270
Fragments arranged by size:
4270 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2908 2908
Size: 5815
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5815
PsiI TTA'TAA
Cuts at: 3385 3385
Size: 5815
PspOMI G'GGCC_C
Cuts at: 2026 2026
Size: 5815
PstI C_TGCA'G
Cuts at: 830 2166 2427 3167 830
Size: 1336 261 740 3478
Fragments arranged by size:
3478 1336 740 261
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5815
PvuII CAG'CTG
Cuts at: 2167 2179 2956 2167
Size: 12 777 5026
Fragments arranged by size:
5026 777 12
SacI G_AGCT'C
Cuts at: 2613 2613
Size: 5815
SacII CC_GC'GG
Cuts at: 2568 2568
Size: 5815
SalI G'TCGA_C
Cuts at: 1936 3157 1936
Size: 1221 4594
SbfI CC_TGCA'GG
Cuts at: 3167 3167
Size: 5815
ScaI AGT'ACT
Cuts at: 4063 5775 4063
Size: 1712 4103
SexAI A'CCwGG_T
Cuts at: 2409 2409
Size: 5815
SfcI C'TryA_G
Cuts at: 826 1045 2162 2203 2423 3163 4298 4979 5170
Size: 219 1117 41 220 740 1135 681 191
Cuts at: 5170 826
Size: 1471
Fragments arranged by size:
1471 1135 1117 740 681 220 219 191
41
SfoI GGC'GCC
Cuts at: 2822 2822
Size: 5815
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5815
SgrAI Cr'CCGG_yG
Cuts at: 1700 2062 2823 1700
Size: 362 761 4692
Fragments arranged by size:
4692 761 362
SmaI CCC'GGG
Cuts at: 1872 3142 1872
Size: 1270 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2612 3919 4820 5082 820
Size: 197 926 669 1307 901 262 1553
Fragments arranged by size:
1553 1307 926 901 669 262 197
SnaBI TAC'GTA
Cuts at: 493 3606 493
Size: 3113 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5815
SphI G_CATG'C
Cuts at: 1132 3173 1132
Size: 2041 3774
SspI AAT'ATT
Cuts at: 5 52 3739 5
Size: 47 3687 2081
Fragments arranged by size:
3687 2081 47
StuI AGG'CCT
Cuts at: 1517 2099 1517
Size: 582 5233
StyI C'CwwG_G
Cuts at: 513 1065 2004 3082 3254 513
Size: 552 939 1078 172 3074
Fragments arranged by size:
3074 1078 939 552 172
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2374 3842 3859 4012 4197 4642 325
Size: 1255 794 1468 17 153 185 445 1498
Fragments arranged by size:
1498 1468 1255 794 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4061 5773 96
Size: 274 80 33 51 1443 2084 1712 138
Fragments arranged by size:
2084 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5775 5807 5775
Size: 32 5783
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5815
XbaI T'CTAG_A
Cuts at: 3151 3151
Size: 5815
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2470 2470
Size: 5815
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5815
XmaI C'CCGG_G
Cuts at: 1870 3140 1870
Size: 1270 4545
XmnI GAAnn'nnTTC
Cuts at: 3944 3944
Size: 5815
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3623 276
Size: 53 83 186 936 2089 2468
Fragments arranged by size:
2468 2089 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI
NruI NsiI PacI PfoI PspXI PsrI RsrII SanDI
SapI SfiI SrfI SwaI