Restriction map for
pFN21AE9511
(Circular) (Six-base) MAPSORT of: pFN21AE9511.seq Check: 1973 from: 1 to: 5689
pFN21AE9511 5689 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3499 278
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2084 2133 3032 1937
Size: 147 49 899 4594
Fragments arranged by size:
4594 899 147 49
Acc65I G'GTAC_C
Cuts at: 3010 3010
Size: 5689
AclI AA'CG_TT
Cuts at: 1563 3816 4189 1563
Size: 2253 373 3063
Fragments arranged by size:
3063 2253 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3752 5136 5404 1632
Size: 2120 1384 268 1917
Fragments arranged by size:
2120 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5492
AflIII A'CryG_T
Cuts at: 1203 2079 2184 4588 5565 1203
Size: 876 105 2404 977 1327
Fragments arranged by size:
2404 1327 977 876 105
AgeI A'CCGG_T
Cuts at: 4520 4520
Size: 5689
AhdI GACnn_n'nnGTC
Cuts at: 4418 4418
Size: 5689
AleI CACnn'nnGTG
Cuts at: 1166 2267 2627 2819 1166
Size: 1101 360 192 4036
Fragments arranged by size:
4036 1101 360 192
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5689
AlwNI CAG_nnn'CTG
Cuts at: 3432 5004 3432
Size: 1572 4117
ApaI G_GGCC'C
Cuts at: 2054 2054
Size: 5689
ApaLI G'TGCA_C
Cuts at: 3746 4902 3746
Size: 1156 4533
ApoI r'AATT_y
Cuts at: 1493 2998 3224 3376 5485 1493
Size: 1505 226 152 2109 1697
Fragments arranged by size:
2109 1697 1505 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4243 160
Size: 872 3211 1606
Fragments arranged by size:
3211 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
AvaI C'yCGr_G
Cuts at: 1870 1943 2588 3014 1870
Size: 73 645 426 4545
Fragments arranged by size:
4545 645 426 73
BamHI G'GATC_C
Cuts at: 3019 3386 3019
Size: 367 5322
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2092 2104 2197 2559 3010
Size: 325 217 627 305 12 93 362 451
Cuts at: 3010 4465 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 451 362 325 305 217
93 12
BanII G_rGCy'C
Cuts at: 1391 2031 2054 2259 2325 3008 1391
Size: 640 23 205 66 683 4072
Fragments arranged by size:
4072 683 640 205 66 23
BbeI G_GCGC'C
Cuts at: 2096 2108 2201 2563 2096
Size: 12 93 362 5222
Fragments arranged by size:
5222 362 93 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2216 2521 928
Size: 971 317 305 4096
Fragments arranged by size:
4096 971 317 305
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2320 2354 3880 3914 1903
Size: 34 383 34 1526 34 3678
Fragments arranged by size:
3678 1526 383 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3581 4791 3581
Size: 1210 4479
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5689
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4300 136
Size: 107 122 71 631 605 2628 1525
Fragments arranged by size:
2628 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5684 5684
Size: 5689
BlpI GC'TnA_GC
Cuts at: 2066 3106 2066
Size: 1040 4649
Bme1580I G_kGCm'C
Cuts at: 1790 2054 3750 4906 1790
Size: 264 1696 1156 2573
Fragments arranged by size:
2573 1696 1156 264
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5689
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3420 4378 442
Size: 416 53 83 399 24 2003 958 1753
Fragments arranged by size:
2003 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5689
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2353 2750 4349 1239
Size: 1114 397 1599 2579
Fragments arranged by size:
2579 1599 1114 397
Bpu10I CC'TnA_GC
Cuts at: 2810 2888 2810
Size: 78 5611
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3814 4679 4977 3814
Size: 865 298 4526
Fragments arranged by size:
4526 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4352 882
Size: 617 2853 2219
Fragments arranged by size:
2853 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2185 3480 493
Size: 711 981 1295 2702
Fragments arranged by size:
2702 1295 981 711
BsaBI GATnn'nnATC
Cuts at: 5683 5683
Size: 5689
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2093 2105 2198 2560
Size: 53 83 186 936 560 12 93 362
Cuts at: 2560 2798 2849 2937 3496 3878 4513 275
Size: 238 51 88 559 382 635 1451
Fragments arranged by size:
1451 936 635 560 559 382 362 238
186 93 88 83 53 51 12
BsaWI w'CCGG_w
Cuts at: 4122 4520 4794 4941 5352 4122
Size: 398 274 147 411 4459
Fragments arranged by size:
4459 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2312 2342 2351 2381 1178
Size: 30 165 30 909 30 9 30 4486
Fragments arranged by size:
4486 909 165 30 30 30 30 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2777 1186
Size: 1591 4098
BseYI C'CCAG_C
Cuts at: 2632 4892 5388 2632
Size: 2260 496 2933
Fragments arranged by size:
2933 2260 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5116
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2404 2452 3139 3900 4928 1540
Size: 275 186 403 48 687 761 1028 2301
Fragments arranged by size:
2301 1028 761 687 403 275 186 48
BsiHKAI G_wGCw'C
Cuts at: 2259 2325 2816 3750 3835 4906 2259
Size: 66 491 934 85 1071 3042
Fragments arranged by size:
3042 1071 934 491 85 66
BsmI GAATG_Cn'
Cuts at: 3200 3293 3200
Size: 93 5596
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2054 2259 2325 2816 3008 3750
Size: 399 241 23 205 66 491 192 742
Cuts at: 3750 3835 4906 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 491 399 241 205 192
85 66 23
BspEI T'CCGG_A
Cuts at: 5352 5352
Size: 5689
BspHI T'CATG_A
Cuts at: 2391 3578 5308 2391
Size: 1187 1730 2772
Fragments arranged by size:
2772 1730 1187
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3044 844
Size: 2200 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3576 1394
Size: 429 1753 3507
Fragments arranged by size:
3507 1753 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4184 4358 65
Size: 1183 2936 174 1396
Fragments arranged by size:
2936 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2101 2447 2556 2688 4333 4520 1700
Size: 110 291 346 109 132 1645 187 2869
Fragments arranged by size:
2869 1645 346 291 187 132 110 109
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5689
BssHII G'CGCG_C
Cuts at: 1925 2468 2470 2605 2734 1925
Size: 543 2 135 129 4880
Fragments arranged by size:
4880 543 135 129 2
BssSI C'ACGA_G
Cuts at: 2627 3749 4761 2627
Size: 1122 1012 3555
Fragments arranged by size:
3555 1122 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2701 794
Size: 1907 3782
BstBI TT'CG_AA
Cuts at: 4493 4493
Size: 5689
BstEII G'GTnAC_C
Cuts at: 1184 2749 1184
Size: 1565 4124
BstXI CCAn_nnnn'nTGG
Cuts at: 2707 2728 2707
Size: 21 5668
BstYI r'GATC_y
Cuts at: 1972 3019 3386 3771 3788 5240 5326 5338 5684
Size: 1047 367 385 17 1452 86 12 346
Cuts at: 5684 1972
Size: 1977
Fragments arranged by size:
1977 1452 1047 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2151 2817 513
Size: 552 939 147 666 3385
Fragments arranged by size:
3385 939 666 552 147
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2230 2269 2845 487
Size: 683 56 1004 39 576 3331
Fragments arranged by size:
3331 1004 683 576 56 39
BtsI GCAGTG_nn'
Cuts at: 2312 2424 3205 3999 4019 2312
Size: 112 781 794 20 3982
Fragments arranged by size:
3982 794 781 112 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5689
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5654
DraI TTT'AAA
Cuts at: 2993 2993
Size: 5689
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4696 809
Size: 507 3380 1802
Fragments arranged by size:
3380 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2241 2421 2445
Size: 54 1425 50 275 18 411 180 24
Cuts at: 2445 2449 2484 3136 4025 5654 8
Size: 4 35 652 889 1629 43
Fragments arranged by size:
1629 1425 889 652 411 275 180 54
50 43 35 24 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2449 3136 1537
Size: 275 637 687 4090
Fragments arranged by size:
4090 687 637 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2264 3619 1353
Size: 911 1355 3423
Fragments arranged by size:
3423 1355 911
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2161 2948 4276 4650 4796 387
Size: 1064 138 572 787 1328 374 146 1280
Fragments arranged by size:
1328 1280 1064 787 572 374 146 138
EcoICRI GAG'CTC
Cuts at: 2257 2323 2257
Size: 66 5623
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2353 2750 3752 4349 5136 5404 1239
Size: 393 721 397 1002 597 787 268 1524
Fragments arranged by size:
1524 1002 787 721 597 397 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2725 2725
Size: 5689
EcoRI G'AATT_C
Cuts at: 2998 3376 2998
Size: 378 5311
FseI GG_CCGG'CC
Cuts at: 2451 2451
Size: 5689
FspI TGC'GCA
Cuts at: 4195 4195
Size: 5689
HaeII r_GCGC'y
Cuts at: 1678 2066 2096 2108 2201 2400 2466 2563 2828
Size: 388 30 12 93 199 66 97 265
Cuts at: 2828 4836 1678
Size: 2008 2531
Fragments arranged by size:
2531 2008 388 265 199 97 93 66
30 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2000 2032 4330 4362 4404
Size: 32 135 32 428 32 2298 32 42
Cuts at: 4404 4436 1373
Size: 32 2626
Fragments arranged by size:
2626 2298 428 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2085 3033 3279 669
Size: 988 281 147 948 246 3079
Fragments arranged by size:
3079 988 948 281 246 147
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5689
HpaI GTT'AAC
Cuts at: 3279 3279
Size: 5689
KasI G'GCGC_C
Cuts at: 2092 2104 2197 2559 2092
Size: 12 93 362 5222
Fragments arranged by size:
5222 362 93 12
KpnI G_GTAC'C
Cuts at: 3014 3014
Size: 5689
MfeI C'AATT_G
Cuts at: 3288 3288
Size: 5689
MluI A'CGCG_T
Cuts at: 2079 5565 2079
Size: 3486 2203
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2100 4803 4987 1305
Size: 795 2703 184 2007
Fragments arranged by size:
2703 2007 795 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2243 2423 10
Size: 54 1425 343 411 180 3276
Fragments arranged by size:
3276 1425 411 343 180 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2267 2390 2627 2648 2819 3647 4006
Size: 648 1101 123 237 21 171 828 359
Cuts at: 4006 4165 518
Size: 159 2042
Fragments arranged by size:
2042 1101 828 648 359 237 171 159
123 21
MspA1I CmG'CkG
Cuts at: 1606 2098 2110 2854 2904 2965 3105 3782 4930
Size: 492 12 744 50 61 140 677 1148
Cuts at: 4930 5175 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 744 677 492 245 140 61
50 12
NaeI GCC'GGC
Cuts at: 2103 2449 2558 2690 2103
Size: 346 109 132 5102
Fragments arranged by size:
5102 346 132 109
NarI GG'CG_CC
Cuts at: 2093 2105 2198 2560 2093
Size: 12 93 362 5222
Fragments arranged by size:
5222 362 93 12
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4198
Fragments arranged by size:
4198 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5689
NgoMIV G'CCGG_C
Cuts at: 2101 2447 2556 2688 2101
Size: 346 109 132 5102
Fragments arranged by size:
5102 346 132 109
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5689
NotI GC'GGCC_GC
Cuts at: 3136 3136
Size: 5689
NspI r_CATG'y
Cuts at: 1132 1229 2699 3047 4592 1132
Size: 97 1470 348 1545 2229
Fragments arranged by size:
2229 1545 1470 348 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5689
PciI A'CATG_T
Cuts at: 4588 4588
Size: 5689
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5689
PfoI T'CCnGG_A
Cuts at: 2931 2931
Size: 5689
PmeI GTTT'AAAC
Cuts at: 2993 2993
Size: 5689
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3758 3790 5271 5303 3758
Size: 32 1481 32 4144
Fragments arranged by size:
4144 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5689
PsiI TTA'TAA
Cuts at: 3259 3259
Size: 5689
PspOMI G'GGCC_C
Cuts at: 2050 2050
Size: 5689
PstI C_TGCA'G
Cuts at: 830 3041 830
Size: 2211 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5689
PvuII CAG'CTG
Cuts at: 2904 2965 2904
Size: 61 5628
SacI G_AGCT'C
Cuts at: 2259 2325 2259
Size: 66 5623
SalI G'TCGA_C
Cuts at: 1936 2083 3031 1936
Size: 147 948 4594
Fragments arranged by size:
4594 948 147
SapI GCTCTTCn'nnn_
Cuts at: 2264 2264
Size: 5689
SbfI CC_TGCA'GG
Cuts at: 3041 3041
Size: 5689
ScaI AGT'ACT
Cuts at: 3937 5649 3937
Size: 1712 3977
SexAI A'CCwGG_T
Cuts at: 2409 2409
Size: 5689
SfcI C'TryA_G
Cuts at: 826 1045 2458 3037 4172 4853 5044 826
Size: 219 1413 579 1135 681 191 1471
Fragments arranged by size:
1471 1413 1135 681 579 219 191
SfoI GGC'GCC
Cuts at: 2094 2106 2199 2561 2094
Size: 12 93 362 5222
Fragments arranged by size:
5222 362 93 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5689
SmaI CCC'GGG
Cuts at: 1872 3016 1872
Size: 1144 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3793 4694 4956 820
Size: 197 926 1850 901 262 1553
Fragments arranged by size:
1850 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3480 493
Size: 2987 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5689
SphI G_CATG'C
Cuts at: 1132 2699 3047 1132
Size: 1567 348 3774
Fragments arranged by size:
3774 1567 348
SspI AAT'ATT
Cuts at: 5 52 3613 5
Size: 47 3561 2081
Fragments arranged by size:
3561 2081 47
StuI AGG'CCT
Cuts at: 1517 2594 1517
Size: 1077 4612
StyI C'CwwG_G
Cuts at: 513 1065 2004 2721 3128 513
Size: 552 939 717 407 3074
Fragments arranged by size:
3074 939 717 552 407
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3716 3733 3886 4071 4516 325
Size: 1255 2136 17 153 185 445 1498
Fragments arranged by size:
2136 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2355 3935 5647
Size: 274 80 33 51 1443 378 1580 1712
Cuts at: 5647 96
Size: 138
Fragments arranged by size:
1712 1580 1443 378 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2312 2344 5649 5681 2312
Size: 32 3305 32 2320
Fragments arranged by size:
3305 2320 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5689
XbaI T'CTAG_A
Cuts at: 3025 3025
Size: 5689
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5689
XmaI C'CCGG_G
Cuts at: 1870 3014 1870
Size: 1144 4545
XmnI GAAnn'nnTTC
Cuts at: 3818 3818
Size: 5689
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3497 276
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV
FalI FspAI NruI NsiI PacI PmlI PpuMI PspXI
PsrI RsrII SacII SanDI SfiI SrfI SwaI XcmI