Restriction map for
pFN21AE9507
(Circular) (Six-base) MAPSORT of: pFN21AE9507.seq Check: 2937 from: 1 to: 5680
pFN21AE9507 5680 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2031 2031
Size: 5680
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3490 278
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3023 1937
Size: 1086 4594
Acc65I G'GTAC_C
Cuts at: 3001 3001
Size: 5680
AclI AA'CG_TT
Cuts at: 1563 3807 4180 1563
Size: 2244 373 3063
Fragments arranged by size:
3063 2244 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3743 5127 5395 1632
Size: 2111 1384 268 1917
Fragments arranged by size:
2111 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5483
AflIII A'CryG_T
Cuts at: 1203 2625 4579 5556 1203
Size: 1422 1954 977 1327
Fragments arranged by size:
1954 1422 1327 977
AgeI A'CCGG_T
Cuts at: 4511 4511
Size: 5680
AhdI GACnn_n'nnGTC
Cuts at: 2078 4409 2078
Size: 2331 3349
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5680
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2410 1184
Size: 1226 4454
AlwNI CAG_nnn'CTG
Cuts at: 2274 3423 4995 2274
Size: 1149 1572 2959
Fragments arranged by size:
2959 1572 1149
ApaI G_GGCC'C
Cuts at: 2761 2761
Size: 5680
ApaLI G'TGCA_C
Cuts at: 3737 4893 3737
Size: 1156 4524
ApoI r'AATT_y
Cuts at: 1493 2008 2989 3215 3367 5476 1493
Size: 515 981 226 152 2109 1697
Fragments arranged by size:
2109 1697 981 515 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4234 160
Size: 872 3202 1606
Fragments arranged by size:
3202 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
AvaI C'yCGr_G
Cuts at: 1870 1943 2672 3005 1870
Size: 73 729 333 4545
Fragments arranged by size:
4545 729 333 73
BamHI G'GATC_C
Cuts at: 3010 3377 3010
Size: 367 5313
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3001 4456 618
Size: 325 217 627 1214 1455 1842
Fragments arranged by size:
1842 1455 1214 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2761 2999 1391
Size: 1370 238 4072
Fragments arranged by size:
4072 1370 238
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2085 2112 2524 2921 928
Size: 971 186 27 412 397 3687
Fragments arranged by size:
3687 971 412 397 186 27
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3871 3905 1903
Size: 34 1934 34 3678
Fragments arranged by size:
3678 1934 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3572 4782 3572
Size: 1210 4470
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5680
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4291 136
Size: 107 122 71 631 605 2619 1525
Fragments arranged by size:
2619 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5675 5675
Size: 5680
BlpI GC'TnA_GC
Cuts at: 3097 3097
Size: 5680
Bme1580I G_kGCm'C
Cuts at: 1790 2761 3741 4897 1790
Size: 971 980 1156 2573
Fragments arranged by size:
2573 1156 980 971
BmgBI CAC'GTC
Cuts at: 1318 2650 1318
Size: 1332 4348
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3411 4369 442
Size: 416 53 83 399 24 1994 958 1753
Fragments arranged by size:
1994 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5680
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4340 1239
Size: 3101 2579
Bpu10I CC'TnA_GC
Cuts at: 2479 2479
Size: 5680
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3805 4670 4968 3805
Size: 865 298 4517
Fragments arranged by size:
4517 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2273 4343 882
Size: 617 774 2070 2219
Fragments arranged by size:
2219 2070 774 617
BsaAI yAC'GTr
Cuts at: 493 1204 3471 493
Size: 711 2267 2702
Fragments arranged by size:
2702 2267 711
BsaBI GATnn'nnATC
Cuts at: 5674 5674
Size: 5680
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2328 3487 3869 4504
Size: 53 83 186 936 795 1159 382 635
Cuts at: 4504 275
Size: 1451
Fragments arranged by size:
1451 1159 936 795 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4113 4511 4785 4932 5343 4113
Size: 398 274 147 411 4450
Fragments arranged by size:
4450 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2192 2222 1178
Size: 30 165 30 789 30 4636
Fragments arranged by size:
4636 789 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5680
BseYI C'CCAG_C
Cuts at: 2734 2754 4883 5379 2734
Size: 20 2129 496 3035
Fragments arranged by size:
3035 2129 496 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2189 2251 2416 2497 1163
Size: 573 453 62 165 81 4346
Fragments arranged by size:
4346 573 453 165 81 62
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3130 3891 4919 1540
Size: 275 186 1129 761 1028 2301
Fragments arranged by size:
2301 1129 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3741 3826 4897 3741
Size: 85 1071 4524
Fragments arranged by size:
4524 1071 85
BsmI GAATG_Cn'
Cuts at: 2053 3191 3284 2053
Size: 1138 93 4449
Fragments arranged by size:
4449 1138 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2761 2999 3741 3826 4897 1391
Size: 399 971 238 742 85 1071 2174
Fragments arranged by size:
2174 1071 971 742 399 238 85
BspEI T'CCGG_A
Cuts at: 5343 5343
Size: 5680
BspHI T'CATG_A
Cuts at: 2463 3569 5299 2463
Size: 1106 1730 2844
Fragments arranged by size:
2844 1730 1106
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2031 2211 3035 844
Size: 1187 180 824 3489
Fragments arranged by size:
3489 1187 824 180
BsrBI CCG'CTC
Cuts at: 1394 1823 3567 1394
Size: 429 1744 3507
Fragments arranged by size:
3507 1744 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2115 2409 4175 4349 65
Size: 1183 867 294 1766 174 1396
Fragments arranged by size:
1766 1396 1183 867 294 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2495 4324 4511 1700
Size: 110 685 1829 187 2869
Fragments arranged by size:
2869 1829 685 187 110
BsrGI T'GTAC_A
Cuts at: 96 2616 96
Size: 2520 3160
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5680
BssSI C'ACGA_G
Cuts at: 3740 4752 3740
Size: 1012 4668
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2520 794
Size: 1726 3954
BstBI TT'CG_AA
Cuts at: 4484 4484
Size: 5680
BstEII G'GTnAC_C
Cuts at: 1184 2716 1184
Size: 1532 4148
BstXI CCAn_nnnn'nTGG
Cuts at: 2416 2734 2416
Size: 318 5362
BstYI r'GATC_y
Cuts at: 1972 2035 3010 3377 3762 3779 5231 5317 5329
Size: 63 975 367 385 17 1452 86 12
Cuts at: 5329 5675 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 975 385 367 346 86 63
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2379 2941 513
Size: 552 1314 562 3252
Fragments arranged by size:
3252 1314 562 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2476 487
Size: 683 56 1250 3691
Fragments arranged by size:
3691 1250 683 56
BtsI GCAGTG_nn'
Cuts at: 2247 2421 3196 3990 4010 2247
Size: 174 775 794 20 3917
Fragments arranged by size:
3917 794 775 174 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5680
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5645
DraI TTT'AAA
Cuts at: 2984 2984
Size: 5680
DraIII CAC_nnn'GTG
Cuts at: 2102 2317 2102
Size: 215 5465
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4687 809
Size: 507 3371 1802
Fragments arranged by size:
3371 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2406 3127 4016
Size: 54 1425 50 275 18 576 721 889
Cuts at: 4016 5645 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 721 576 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3127 1537
Size: 275 1315 4090
Fragments arranged by size:
4090 1315 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3610 1353
Size: 2257 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2261 4267 4641 4787 387
Size: 1064 138 672 2006 374 146 1280
Fragments arranged by size:
2006 1280 1064 672 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3743 4340 5127 5395 1239
Size: 393 2111 597 787 268 1524
Fragments arranged by size:
2111 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2072 2758 2072
Size: 686 4994
EcoRI G'AATT_C
Cuts at: 2008 2989 3367 2008
Size: 981 378 4321
Fragments arranged by size:
4321 981 378
FspI TGC'GCA
Cuts at: 4186 4186
Size: 5680
HaeII r_GCGC'y
Cuts at: 1678 2479 4827 1678
Size: 801 2348 2531
Fragments arranged by size:
2531 2348 801
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2064 2096 2650 2682 2863
Size: 32 135 32 492 32 554 32 181
Cuts at: 2863 2895 4321 4353 4395 4427 1373
Size: 32 1426 32 42 32 2626
Fragments arranged by size:
2626 1426 554 492 181 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3024 3270 669
Size: 988 281 1086 246 3079
Fragments arranged by size:
3079 1086 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5680
HpaI GTT'AAC
Cuts at: 3270 3270
Size: 5680
KpnI G_GTAC'C
Cuts at: 3005 3005
Size: 5680
MfeI C'AATT_G
Cuts at: 3279 3279
Size: 5680
MluI A'CGCG_T
Cuts at: 5556 5556
Size: 5680
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4794 4978 1305
Size: 3489 184 2007
Fragments arranged by size:
3489 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2408 10
Size: 54 1425 343 576 3282
Fragments arranged by size:
3282 1425 576 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2120 2255 2378 2392 2414 2579 2624
Size: 648 954 135 123 14 22 165 45
Cuts at: 2624 2783 3638 3997 4156 518
Size: 159 855 359 159 2042
Fragments arranged by size:
2042 954 855 648 359 165 159 159
135 123 45 22 14
MspA1I CmG'CkG
Cuts at: 1606 3096 3773 4921 5166 1606
Size: 1490 677 1148 245 2120
Fragments arranged by size:
2120 1490 1148 677 245
NaeI GCC'GGC
Cuts at: 2497 2497
Size: 5680
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5128
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5680
NgoMIV G'CCGG_C
Cuts at: 2495 2495
Size: 5680
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5680
NotI GC'GGCC_GC
Cuts at: 3127 3127
Size: 5680
NspI r_CATG'y
Cuts at: 1132 1229 2254 2266 2629 2707 3038 4583 1132
Size: 97 1025 12 363 78 331 1545 2229
Fragments arranged by size:
2229 1545 1025 363 331 97 78 12
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5680
PciI A'CATG_T
Cuts at: 2625 4579 2625
Size: 1954 3726
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2274 1108
Size: 1166 4514
PmeI GTTT'AAAC
Cuts at: 2984 2984
Size: 5680
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3749 3781 5262 5294 3749
Size: 32 1481 32 4135
Fragments arranged by size:
4135 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2072 2072
Size: 5680
PshAI GACnn'nnGTC
Cuts at: 1613 2648 1613
Size: 1035 4645
PsiI TTA'TAA
Cuts at: 3250 3250
Size: 5680
PspOMI G'GGCC_C
Cuts at: 2757 2757
Size: 5680
PstI C_TGCA'G
Cuts at: 830 2963 3032 830
Size: 2133 69 3478
Fragments arranged by size:
3478 2133 69
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5680
SalI G'TCGA_C
Cuts at: 1936 3022 1936
Size: 1086 4594
SbfI CC_TGCA'GG
Cuts at: 3032 3032
Size: 5680
ScaI AGT'ACT
Cuts at: 3928 5640 3928
Size: 1712 3968
SfcI C'TryA_G
Cuts at: 826 1045 2959 3028 4163 4844 5035 826
Size: 219 1914 69 1135 681 191 1471
Fragments arranged by size:
1914 1471 1135 681 219 191 69
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5680
SmaI CCC'GGG
Cuts at: 1872 2674 3007 1872
Size: 802 333 4545
Fragments arranged by size:
4545 802 333
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3784 4685 4947 820
Size: 197 926 1841 901 262 1553
Fragments arranged by size:
1841 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3471 493
Size: 2978 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5680
SphI G_CATG'C
Cuts at: 1132 2707 3038 1132
Size: 1575 331 3774
Fragments arranged by size:
3774 1575 331
SspI AAT'ATT
Cuts at: 5 52 3604 5
Size: 47 3552 2081
Fragments arranged by size:
3552 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5680
StyI C'CwwG_G
Cuts at: 513 1065 3119 513
Size: 552 2054 3074
Fragments arranged by size:
3074 2054 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2132 3707 3724 3877 4062 4507 325
Size: 1255 552 1575 17 153 185 445 1498
Fragments arranged by size:
1575 1498 1255 552 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2217 2541 2616
Size: 274 80 33 51 1443 240 324 75
Cuts at: 2616 3926 5638 96
Size: 1310 1712 138
Fragments arranged by size:
1712 1443 1310 324 274 240 138 80
75 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5640 5672 5640
Size: 32 5648
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5680
XbaI T'CTAG_A
Cuts at: 3016 3016
Size: 5680
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5680
XmaI C'CCGG_G
Cuts at: 1870 2672 3005 1870
Size: 802 333 4545
Fragments arranged by size:
4545 802 333
XmnI GAAnn'nnTTC
Cuts at: 3809 3809
Size: 5680
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3488 276
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI