Restriction map for
pFN21AE9506
(Circular) (Six-base) MAPSORT of: pFN21AE9506.seq Check: 7245 from: 1 to: 5701
pFN21AE9506 5701 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3511 278
Size: 53 83 186 936 1975 2468
Fragments arranged by size:
2468 1975 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3044 1937
Size: 1107 4594
Acc65I G'GTAC_C
Cuts at: 3022 3022
Size: 5701
AclI AA'CG_TT
Cuts at: 1563 3828 4201 1563
Size: 2265 373 3063
Fragments arranged by size:
3063 2265 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3764 5148 5416 1632
Size: 2132 1384 268 1917
Fragments arranged by size:
2132 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5504
AflIII A'CryG_T
Cuts at: 1203 2646 4600 5577 1203
Size: 1443 1954 977 1327
Fragments arranged by size:
1954 1443 1327 977
AgeI A'CCGG_T
Cuts at: 4532 4532
Size: 5701
AhdI GACnn_n'nnGTC
Cuts at: 4430 4430
Size: 5701
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5701
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2097 2431 1184
Size: 913 334 4454
Fragments arranged by size:
4454 913 334
AlwNI CAG_nnn'CTG
Cuts at: 3444 5016 3444
Size: 1572 4129
ApaI G_GGCC'C
Cuts at: 2782 2782
Size: 5701
ApaLI G'TGCA_C
Cuts at: 3758 4914 3758
Size: 1156 4545
ApoI r'AATT_y
Cuts at: 1493 3010 3236 3388 5497 1493
Size: 1517 226 152 2109 1697
Fragments arranged by size:
2109 1697 1517 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4255 160
Size: 872 3223 1606
Fragments arranged by size:
3223 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5701
AvaI C'yCGr_G
Cuts at: 1870 1943 3026 1870
Size: 73 1083 4545
Fragments arranged by size:
4545 1083 73
BamHI G'GATC_C
Cuts at: 3031 3398 3031
Size: 367 5334
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2702 2717 3022 4477 618
Size: 325 217 627 915 15 305 1455 1842
Fragments arranged by size:
1842 1455 915 627 325 305 217 15
BanII G_rGCy'C
Cuts at: 1391 2782 3020 1391
Size: 1391 238 4072
Fragments arranged by size:
4072 1391 238
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4730
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3892 3926 1903
Size: 34 1955 34 3678
Fragments arranged by size:
3678 1955 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3593 4803 3593
Size: 1210 4491
BclI T'GATC_A
Cuts at: 1548 2874 1548
Size: 1326 4375
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4312 136
Size: 107 122 71 631 605 2640 1525
Fragments arranged by size:
2640 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5696 5696
Size: 5701
BlpI GC'TnA_GC
Cuts at: 3118 3118
Size: 5701
Bme1580I G_kGCm'C
Cuts at: 1790 2782 3762 4918 1790
Size: 992 980 1156 2573
Fragments arranged by size:
2573 1156 992 980
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5701
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3432 4390 442
Size: 416 53 83 399 24 2015 958 1753
Fragments arranged by size:
2015 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5701
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2453 4361 1239
Size: 1214 1908 2579
Fragments arranged by size:
2579 1908 1214
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3826 4691 4989 3826
Size: 865 298 4538
Fragments arranged by size:
4538 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4364 882
Size: 617 2865 2219
Fragments arranged by size:
2865 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3492 493
Size: 711 2288 2702
Fragments arranged by size:
2702 2288 711
BsaBI GATnn'nnATC
Cuts at: 5695 5695
Size: 5701
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2760 3508 3890 4525
Size: 53 83 186 936 1227 748 382 635
Cuts at: 4525 275
Size: 1451
Fragments arranged by size:
1451 1227 936 748 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2916 4134 4532 4806 4953 5364 2916
Size: 1218 398 274 147 411 3253
Fragments arranged by size:
3253 1218 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5476
Fragments arranged by size:
5476 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5701
BseYI C'CCAG_C
Cuts at: 2775 4904 5400 2775
Size: 2129 496 3076
Fragments arranged by size:
3076 2129 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2027 2057 1163
Size: 573 291 30 4807
Fragments arranged by size:
4807 573 291 30
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3151 3912 4940 1540
Size: 275 186 1150 761 1028 2301
Fragments arranged by size:
2301 1150 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3762 3847 4918 3762
Size: 85 1071 4545
Fragments arranged by size:
4545 1071 85
BsmI GAATG_Cn'
Cuts at: 2230 3212 3305 2230
Size: 982 93 4626
Fragments arranged by size:
4626 982 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2782 3020 3762 3847 4918 1391
Size: 399 992 238 742 85 1071 2174
Fragments arranged by size:
2174 1071 992 742 399 238 85
BspEI T'CCGG_A
Cuts at: 2916 5364 2916
Size: 2448 3253
BspHI T'CATG_A
Cuts at: 3590 5320 3590
Size: 1730 3971
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3056 844
Size: 2212 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3588 1394
Size: 429 1765 3507
Fragments arranged by size:
3507 1765 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2086 4196 4370 65
Size: 1183 838 2110 174 1396
Fragments arranged by size:
2110 1396 1183 838 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4345 4532 1700
Size: 110 2535 187 2869
Fragments arranged by size:
2869 2535 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5701
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5701
BssSI C'ACGA_G
Cuts at: 3761 4773 3761
Size: 1012 4689
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5701
BstBI TT'CG_AA
Cuts at: 4505 4505
Size: 5701
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5701
BstYI r'GATC_y
Cuts at: 1972 3031 3398 3783 3800 5252 5338 5350 5696
Size: 1059 367 385 17 1452 86 12 346
Cuts at: 5696 1972
Size: 1977
Fragments arranged by size:
1977 1452 1059 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2148 2148
Size: 5701
BtgI C'CryG_G
Cuts at: 513 1065 2004 2023 513
Size: 552 939 19 4191
Fragments arranged by size:
4191 939 552 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4962
Fragments arranged by size:
4962 683 56
BtsI GCAGTG_nn'
Cuts at: 2442 3217 4011 4031 2442
Size: 775 794 20 4112
Fragments arranged by size:
4112 794 775 20
ClaI AT'CG_AT
Cuts at: 1554 2895 1554
Size: 1341 4360
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2418 2453 529
Size: 35 1854 35 3777
Fragments arranged by size:
3777 1854 35 35
DraI TTT'AAA
Cuts at: 3005 3005
Size: 5701
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4708 809
Size: 507 3392 1802
Fragments arranged by size:
3392 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2658 3148 4037
Size: 54 1425 50 275 18 828 490 889
Cuts at: 4037 5666 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 828 490 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3148 1537
Size: 275 1336 4090
Fragments arranged by size:
4090 1336 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3631 1353
Size: 2278 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2699 4288 4662 4808 387
Size: 1064 138 1110 1589 374 146 1280
Fragments arranged by size:
1589 1280 1110 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2453 3764 4361 5148 5416 1239
Size: 393 821 1311 597 787 268 1524
Fragments arranged by size:
1524 1311 821 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2779 2779
Size: 5701
EcoRI G'AATT_C
Cuts at: 3010 3388 3010
Size: 378 5323
FspI TGC'GCA
Cuts at: 4207 4207
Size: 5701
HaeII r_GCGC'y
Cuts at: 1678 4848 1678
Size: 3170 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4342 4374 4416 4448 1373
Size: 32 135 32 2770 32 42 32 2626
Fragments arranged by size:
2770 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3045 3291 669
Size: 988 281 1107 246 3079
Fragments arranged by size:
3079 1107 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5701
HpaI GTT'AAC
Cuts at: 3291 3291
Size: 5701
KpnI G_GTAC'C
Cuts at: 3026 3026
Size: 5701
MfeI C'AATT_G
Cuts at: 2430 3300 2430
Size: 870 4831
MluI A'CGCG_T
Cuts at: 5577 5577
Size: 5701
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2163 4815 4999 1305
Size: 858 2652 184 2007
Fragments arranged by size:
2652 2007 858 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2660 10
Size: 54 1425 343 828 3051
Fragments arranged by size:
3051 1425 828 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2079 2600 3659 4018 4177 518
Size: 648 913 521 1059 359 159 2042
Fragments arranged by size:
2042 1059 913 648 521 359 159
MspA1I CmG'CkG
Cuts at: 1606 3117 3794 4942 5187 1606
Size: 1511 677 1148 245 2120
Fragments arranged by size:
2120 1511 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4210
Fragments arranged by size:
4210 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5701
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5701
NotI GC'GGCC_GC
Cuts at: 3148 3148
Size: 5701
NspI r_CATG'y
Cuts at: 1132 1229 2650 3059 4604 1132
Size: 97 1421 409 1545 2229
Fragments arranged by size:
2229 1545 1421 409 97
PasI CC'CwG_GG
Cuts at: 1348 2968 1348
Size: 1620 4081
PciI A'CATG_T
Cuts at: 2646 4600 2646
Size: 1954 3747
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5701
PmeI GTTT'AAAC
Cuts at: 3005 3005
Size: 5701
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3770 3802 5283 5315 3770
Size: 32 1481 32 4156
Fragments arranged by size:
4156 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5701
PsiI TTA'TAA
Cuts at: 3271 3271
Size: 5701
PspOMI G'GGCC_C
Cuts at: 2778 2778
Size: 5701
PstI C_TGCA'G
Cuts at: 830 2258 3053 830
Size: 1428 795 3478
Fragments arranged by size:
3478 1428 795
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5701
SalI G'TCGA_C
Cuts at: 1936 3043 1936
Size: 1107 4594
SbfI CC_TGCA'GG
Cuts at: 3053 3053
Size: 5701
ScaI AGT'ACT
Cuts at: 3949 5661 3949
Size: 1712 3989
SfcI C'TryA_G
Cuts at: 826 1045 2254 2392 3049 4184 4865 5056 826
Size: 219 1209 138 657 1135 681 191 1471
Fragments arranged by size:
1471 1209 1135 681 657 219 191 138
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5701
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5701
SmaI CCC'GGG
Cuts at: 1872 3028 1872
Size: 1156 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3805 4706 4968 820
Size: 197 926 1862 901 262 1553
Fragments arranged by size:
1862 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3492 493
Size: 2999 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5701
SphI G_CATG'C
Cuts at: 1132 3059 1132
Size: 1927 3774
SspI AAT'ATT
Cuts at: 5 52 2365 2802 3625 5
Size: 47 2313 437 823 2081
Fragments arranged by size:
2313 2081 823 437 47
StuI AGG'CCT
Cuts at: 1517 2976 1517
Size: 1459 4242
StyI C'CwwG_G
Cuts at: 513 1065 2004 2095 2713 3140 513
Size: 552 939 91 618 427 3074
Fragments arranged by size:
3074 939 618 552 427 91
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2033 2156 2251 3728 3745 3898 4083
Size: 1255 453 123 95 1477 17 153 185
Cuts at: 4083 4528 325
Size: 445 1498
Fragments arranged by size:
1498 1477 1255 453 445 185 153 123
95 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2241 2330 3947
Size: 274 80 33 51 1443 264 89 1617
Cuts at: 3947 5659 96
Size: 1712 138
Fragments arranged by size:
1712 1617 1443 274 264 138 89 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5661 5693 5661
Size: 32 5669
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5701
XbaI T'CTAG_A
Cuts at: 3037 3037
Size: 5701
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5701
XmaI C'CCGG_G
Cuts at: 1870 3026 1870
Size: 1156 4545
XmnI GAAnn'nnTTC
Cuts at: 2939 3830 2939
Size: 891 4810
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3509 276
Size: 53 83 186 936 1975 2468
Fragments arranged by size:
2468 1975 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI