Restriction map for
pFN21AE9505
(Circular) (Six-base) MAPSORT of: pFN21AE9505.seq Check: 6580 from: 1 to: 5716
pFN21AE9505 5716 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2449 2763 2449
Size: 314 5402
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3526 278
Size: 53 83 186 936 1990 2468
Fragments arranged by size:
2468 1990 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2432 3059 1937
Size: 495 627 4594
Fragments arranged by size:
4594 627 495
Acc65I G'GTAC_C
Cuts at: 3037 3037
Size: 5716
AclI AA'CG_TT
Cuts at: 1563 3843 4216 1563
Size: 2280 373 3063
Fragments arranged by size:
3063 2280 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3779 5163 5431 1632
Size: 2147 1384 268 1917
Fragments arranged by size:
2147 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5519
AflIII A'CryG_T
Cuts at: 1203 4615 5592 1203
Size: 3412 977 1327
Fragments arranged by size:
3412 1327 977
AgeI A'CCGG_T
Cuts at: 4547 4547
Size: 5716
AhdI GACnn_n'nnGTC
Cuts at: 4445 4445
Size: 5716
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5716
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5716
AlwNI CAG_nnn'CTG
Cuts at: 3459 5031 3459
Size: 1572 4144
ApaI G_GGCC'C
Cuts at: 2640 2812 2935 2640
Size: 172 123 5421
Fragments arranged by size:
5421 172 123
ApaLI G'TGCA_C
Cuts at: 3773 4929 3773
Size: 1156 4560
ApoI r'AATT_y
Cuts at: 1493 3025 3251 3403 5512 1493
Size: 1532 226 152 2109 1697
Fragments arranged by size:
2109 1697 1532 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4270 160
Size: 872 3238 1606
Fragments arranged by size:
3238 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5716
AvaI C'yCGr_G
Cuts at: 1870 1943 2414 3041 1870
Size: 73 471 627 4545
Fragments arranged by size:
4545 627 471 73
BamHI G'GATC_C
Cuts at: 3046 3413 3046
Size: 367 5349
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2081 2152 2210 2743 3037
Size: 325 217 627 294 71 58 533 294
Cuts at: 3037 4492 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 533 325 294 294 217
71 58
BanII G_rGCy'C
Cuts at: 1391 2640 2812 2896 2935 3035 1391
Size: 1249 172 84 39 100 4072
Fragments arranged by size:
4072 1249 172 100 84 39
BbeI G_GCGC'C
Cuts at: 2085 2085
Size: 5716
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4745
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3907 3941 1903
Size: 34 1970 34 3678
Fragments arranged by size:
3678 1970 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3608 4818 3608
Size: 1210 4506
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5716
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2079 2090 2171
Size: 107 122 71 631 605 407 11 81
Cuts at: 2171 2180 2471 2816 4327 136
Size: 9 291 345 1511 1525
Fragments arranged by size:
1525 1511 631 605 407 345 291 122
107 81 71 11 9
BglII A'GATC_T
Cuts at: 5711 5711
Size: 5716
BlpI GC'TnA_GC
Cuts at: 3133 3133
Size: 5716
Bme1580I G_kGCm'C
Cuts at: 1790 2283 2508 2640 2671 2711 2746 2812 2935
Size: 493 225 132 31 40 35 66 123
Cuts at: 2935 3777 4933 1790
Size: 842 1156 2573
Fragments arranged by size:
2573 1156 842 493 225 132 123 66
40 35 31
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5716
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2938 3447 4405
Size: 416 53 83 399 24 1521 509 958
Cuts at: 4405 442
Size: 1753
Fragments arranged by size:
1753 1521 958 509 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5716
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2569 4376 1239
Size: 1330 1807 2579
Fragments arranged by size:
2579 1807 1330
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3841 4706 5004 3841
Size: 865 298 4553
Fragments arranged by size:
4553 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3004 4379 882
Size: 617 1505 1375 2219
Fragments arranged by size:
2219 1505 1375 617
BsaAI yAC'GTr
Cuts at: 493 1204 3507 493
Size: 711 2303 2702
Fragments arranged by size:
2702 2303 711
BsaBI GATnn'nnATC
Cuts at: 5710 5710
Size: 5716
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2082 3523 3905 4540
Size: 53 83 186 936 549 1441 382 635
Cuts at: 4540 275
Size: 1451
Fragments arranged by size:
1451 1441 936 635 549 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2613 4149 4547 4821 4968 5379 2613
Size: 1536 398 274 147 411 2950
Fragments arranged by size:
2950 1536 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2194 2224 2564 2594 1178
Size: 30 165 30 791 30 340 30 4300
Fragments arranged by size:
4300 791 340 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2572 1186
Size: 1386 4330
BseYI C'CCAG_C
Cuts at: 2183 2545 2679 2704 2739 2758 4919 5415 2183
Size: 362 134 25 35 19 2161 496 2484
Fragments arranged by size:
2484 2161 496 362 134 35 25 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2334 2477 2513 1163
Size: 573 598 143 36 4366
Fragments arranged by size:
4366 598 573 143 36
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2702 3166 3927 4955 1540
Size: 275 186 701 464 761 1028 2301
Fragments arranged by size:
2301 1028 761 701 464 275 186
BsiHKAI G_wGCw'C
Cuts at: 2541 2772 3777 3862 4933 2541
Size: 231 1005 85 1071 3324
Fragments arranged by size:
3324 1071 1005 231 85
BsmI GAATG_Cn'
Cuts at: 2602 3227 3320 2602
Size: 625 93 4998
Fragments arranged by size:
4998 625 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2283 2508 2541 2640 2671 2711 2746
Size: 399 493 225 33 99 31 40 35
Cuts at: 2746 2772 2812 2896 2935 3035 3777 3862 4933
Size: 26 40 84 39 100 742 85 1071
Cuts at: 4933 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 493 399 225 100 99
85 84 40 40 39 35 33 31
26
BspEI T'CCGG_A
Cuts at: 5379 5379
Size: 5716
BspHI T'CATG_A
Cuts at: 3605 5335 3605
Size: 1730 3986
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2449 2763 3071 844
Size: 1605 314 308 3489
Fragments arranged by size:
3489 1605 314 308
BsrBI CCG'CTC
Cuts at: 1394 1823 2119 3603 1394
Size: 429 296 1484 3507
Fragments arranged by size:
3507 1484 429 296
BsrDI GCAATG_nn'
Cuts at: 65 1248 2167 2283 4211 4385 65
Size: 1183 919 116 1928 174 1396
Fragments arranged by size:
1928 1396 1183 919 174 116
BsrFI r'CCGG_y
Cuts at: 1700 1810 2523 2697 2904 4360 4547 1700
Size: 110 713 174 207 1456 187 2869
Fragments arranged by size:
2869 1456 713 207 187 174 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5716
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5716
BssSI C'ACGA_G
Cuts at: 3776 4788 3776
Size: 1012 4704
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5716
BstBI TT'CG_AA
Cuts at: 4520 4520
Size: 5716
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5716
BstXI CCAn_nnnn'nTGG
Cuts at: 2143 2722 2143
Size: 579 5137
BstYI r'GATC_y
Cuts at: 1972 3046 3413 3798 3815 5267 5353 5365 5711
Size: 1074 367 385 17 1452 86 12 346
Cuts at: 5711 1972
Size: 1977
Fragments arranged by size:
1977 1452 1074 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2936 2936
Size: 5716
BtgI C'CryG_G
Cuts at: 513 1065 2004 2166 2235 2290 2649 513
Size: 552 939 162 69 55 359 3580
Fragments arranged by size:
3580 939 552 359 162 69 55
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4977
Fragments arranged by size:
4977 683 56
BtsI GCAGTG_nn'
Cuts at: 2283 2322 2564 2909 3232 4026 4046 2283
Size: 39 242 345 323 794 20 3953
Fragments arranged by size:
3953 794 345 323 242 39 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5716
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5681
DraI TTT'AAA
Cuts at: 3020 3020
Size: 5716
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4723 809
Size: 507 3407 1802
Fragments arranged by size:
3407 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2091 2232 2303
Size: 54 1425 50 275 18 261 141 71
Cuts at: 2303 2646 2652 2699 2802 3163 4052 5681 8
Size: 343 6 47 103 361 889 1629 43
Fragments arranged by size:
1629 1425 889 361 343 275 261 141
103 71 54 50 47 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 2699 3163 1537
Size: 275 887 464 4090
Fragments arranged by size:
4090 887 464 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2879 3646 1353
Size: 1526 767 3423
Fragments arranged by size:
3423 1526 767
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2255 2500 2891 4303 4677 4823
Size: 1064 138 666 245 391 1412 374 146
Cuts at: 4823 387
Size: 1280
Fragments arranged by size:
1412 1280 1064 666 391 374 245 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2569 3779 4376 5163 5431 1239
Size: 393 937 1210 597 787 268 1524
Fragments arranged by size:
1524 1210 937 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2636 2637 2808 2809 2932 2636
Size: 1 171 1 123 5420
Fragments arranged by size:
5420 171 123 1 1
EcoRI G'AATT_C
Cuts at: 3025 3403 3025
Size: 378 5338
FspI TGC'GCA
Cuts at: 4222 4222
Size: 5716
HaeII r_GCGC'y
Cuts at: 1678 2085 2333 4863 1678
Size: 407 248 2530 2531
Fragments arranged by size:
2531 2530 407 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2984 3016 4357 4389 4431
Size: 32 135 32 1412 32 1341 32 42
Cuts at: 4431 4463 1373
Size: 32 2626
Fragments arranged by size:
2626 1412 1341 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3060 3306 669
Size: 988 281 1122 246 3079
Fragments arranged by size:
3079 1122 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5716
HpaI GTT'AAC
Cuts at: 3306 3306
Size: 5716
KasI G'GCGC_C
Cuts at: 2081 2081
Size: 5716
KpnI G_GTAC'C
Cuts at: 3041 3041
Size: 5716
MfeI C'AATT_G
Cuts at: 3315 3315
Size: 5716
MluI A'CGCG_T
Cuts at: 5592 5592
Size: 5716
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4830 5014 1305
Size: 3525 184 2007
Fragments arranged by size:
3525 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2093 2654 2804 10
Size: 54 1425 343 261 561 150 2922
Fragments arranged by size:
2922 1425 561 343 261 150 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2720 3674 4033 4192 518
Size: 648 1554 954 359 159 2042
Fragments arranged by size:
2042 1554 954 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2104 2443 2658 2704 2856 2918 3132 3809
Size: 498 339 215 46 152 62 214 677
Cuts at: 3809 4957 5202 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 498 339 245 215 214
152 62 46
NaeI GCC'GGC
Cuts at: 2525 2699 2525
Size: 174 5542
NarI GG'CG_CC
Cuts at: 2082 2082
Size: 5716
NcoI C'CATG_G
Cuts at: 513 1065 2004 2166 513
Size: 552 939 162 4063
Fragments arranged by size:
4063 939 552 162
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5716
NgoMIV G'CCGG_C
Cuts at: 2523 2697 2523
Size: 174 5542
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5716
NotI GC'GGCC_GC
Cuts at: 3163 3163
Size: 5716
NspI r_CATG'y
Cuts at: 1132 1229 3074 4619 1132
Size: 97 1845 1545 2229
Fragments arranged by size:
2229 1845 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2947 1348
Size: 1599 4117
PciI A'CATG_T
Cuts at: 4615 4615
Size: 5716
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2758 2995 1108
Size: 1650 237 3829
Fragments arranged by size:
3829 1650 237
PmeI GTTT'AAAC
Cuts at: 3020 3020
Size: 5716
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3785 3817 5298 5330 3785
Size: 32 1481 32 4171
Fragments arranged by size:
4171 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5716
PsiI TTA'TAA
Cuts at: 3286 3286
Size: 5716
PspOMI G'GGCC_C
Cuts at: 2636 2808 2931 2636
Size: 172 123 5421
Fragments arranged by size:
5421 172 123
PstI C_TGCA'G
Cuts at: 830 2559 3068 830
Size: 1729 509 3478
Fragments arranged by size:
3478 1729 509
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5716
PvuII CAG'CTG
Cuts at: 2658 2856 2918 2658
Size: 198 62 5456
Fragments arranged by size:
5456 198 62
RsrII CG'GwC_CG
Cuts at: 2056 2056
Size: 5716
SalI G'TCGA_C
Cuts at: 1936 3058 1936
Size: 1122 4594
SapI GCTCTTCn'nnn_
Cuts at: 2879 2879
Size: 5716
SbfI CC_TGCA'GG
Cuts at: 3068 3068
Size: 5716
ScaI AGT'ACT
Cuts at: 3964 5676 3964
Size: 1712 4004
SexAI A'CCwGG_T
Cuts at: 2216 2994 2216
Size: 778 4938
SfcI C'TryA_G
Cuts at: 826 1045 2555 3064 4199 4880 5071 826
Size: 219 1510 509 1135 681 191 1471
Fragments arranged by size:
1510 1471 1135 681 509 219 191
SfoI GGC'GCC
Cuts at: 2083 2083
Size: 5716
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5716
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5716
SmaI CCC'GGG
Cuts at: 1872 3043 1872
Size: 1171 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3820 4721 4983 820
Size: 197 926 1877 901 262 1553
Fragments arranged by size:
1877 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3507 493
Size: 3014 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5716
SphI G_CATG'C
Cuts at: 1132 3074 1132
Size: 1942 3774
SspI AAT'ATT
Cuts at: 5 52 3640 5
Size: 47 3588 2081
Fragments arranged by size:
3588 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5716
StyI C'CwwG_G
Cuts at: 513 1065 2004 2166 3155 513
Size: 552 939 162 989 3074
Fragments arranged by size:
3074 989 939 552 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2167 2198 3743 3760 3913 4098 4543
Size: 1255 587 31 1545 17 153 185 445
Cuts at: 4543 325
Size: 1498
Fragments arranged by size:
1545 1498 1255 587 445 185 153 31
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3962 5674 96
Size: 274 80 33 51 1443 1985 1712 138
Fragments arranged by size:
1985 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2194 2226 2234 2266 5676 5708 2194
Size: 32 8 32 3410 32 2202
Fragments arranged by size:
3410 2202 32 32 32 8
Tth111I GACn'n_nGTC
Cuts at: 1316 2996 1316
Size: 1680 4036
XbaI T'CTAG_A
Cuts at: 3052 3052
Size: 5716
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2143 2306 2992 2143
Size: 163 686 4867
Fragments arranged by size:
4867 686 163
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5716
XmaI C'CCGG_G
Cuts at: 1870 3041 1870
Size: 1171 4545
XmnI GAAnn'nnTTC
Cuts at: 3845 3845
Size: 5716
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3524 276
Size: 53 83 186 936 1990 2468
Fragments arranged by size:
2468 1990 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII RsrII
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI NruI NsiI PacI PfoI PmlI
PpuMI PspXI PsrI SacI SacII SanDI SfiI SrfI
SwaI