Restriction map for
pFN21AE9504
(Circular) (Six-base) MAPSORT of: pFN21AE9504.seq Check: 4003 from: 1 to: 5869
pFN21AE9504 5869 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3679 278
Size: 53 83 186 936 2143 2468
Fragments arranged by size:
2468 2143 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2379 2643 3212 1937
Size: 442 264 569 4594
Fragments arranged by size:
4594 569 442 264
Acc65I G'GTAC_C
Cuts at: 3190 3190
Size: 5869
AclI AA'CG_TT
Cuts at: 1563 3996 4369 1563
Size: 2433 373 3063
Fragments arranged by size:
3063 2433 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3932 5316 5584 1632
Size: 2300 1384 268 1917
Fragments arranged by size:
2300 1917 1384 268
AfeI AGC'GCT
Cuts at: 2086 2086
Size: 5869
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5672
AflIII A'CryG_T
Cuts at: 1203 2391 2886 4768 5745 1203
Size: 1188 495 1882 977 1327
Fragments arranged by size:
1882 1327 1188 977 495
AgeI A'CCGG_T
Cuts at: 4700 4700
Size: 5869
AhdI GACnn_n'nnGTC
Cuts at: 4598 4598
Size: 5869
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5869
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5869
AlwNI CAG_nnn'CTG
Cuts at: 2845 3612 5184 2845
Size: 767 1572 3530
Fragments arranged by size:
3530 1572 767
ApaI G_GGCC'C
Cuts at: 2121 2121
Size: 5869
ApaLI G'TGCA_C
Cuts at: 2450 3926 5082 2450
Size: 1476 1156 3237
Fragments arranged by size:
3237 1476 1156
ApoI r'AATT_y
Cuts at: 1493 3178 3404 3556 5665 1493
Size: 1685 226 152 2109 1697
Fragments arranged by size:
2109 1697 1685 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4423 160
Size: 872 3391 1606
Fragments arranged by size:
3391 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5869
AvaI C'yCGr_G
Cuts at: 1870 1943 2030 2120 3194 1870
Size: 73 87 90 1074 4545
Fragments arranged by size:
4545 1074 90 87 73
BamHI G'GATC_C
Cuts at: 3199 3566 3199
Size: 367 5502
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3190 4645 618
Size: 325 217 627 1403 1455 1842
Fragments arranged by size:
1842 1455 1403 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2121 2289 3033 3188 1391
Size: 730 168 744 155 4072
Fragments arranged by size:
4072 744 730 168 155
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2246 2398 2626 928
Size: 971 347 152 228 4171
Fragments arranged by size:
4171 971 347 228 152
BbvCI CC'TCA_GC
Cuts at: 2900 3021 2900
Size: 121 5748
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4060 4094 1903
Size: 34 2123 34 3678
Fragments arranged by size:
3678 2123 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3761 4971 3761
Size: 1210 4659
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5869
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2321 2627 4480
Size: 107 122 71 631 605 649 306 1853
Cuts at: 4480 136
Size: 1525
Fragments arranged by size:
1853 1525 649 631 605 306 122 107
71
BglII A'GATC_T
Cuts at: 5864 5864
Size: 5869
BlpI GC'TnA_GC
Cuts at: 2297 2372 2423 3286 2297
Size: 75 51 863 4880
Fragments arranged by size:
4880 863 75 51
Bme1580I G_kGCm'C
Cuts at: 1790 2121 2307 2454 3089 3930 5086 1790
Size: 331 186 147 635 841 1156 2573
Fragments arranged by size:
2573 1156 841 635 331 186 147
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5869
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3600 4558 442
Size: 416 53 83 399 24 2183 958 1753
Fragments arranged by size:
2183 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5869
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3033 4529 1239
Size: 1794 1496 2579
Fragments arranged by size:
2579 1794 1496
Bpu10I CC'TnA_GC
Cuts at: 2900 3021 2900
Size: 121 5748
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3041 3994 4859 5157 3041
Size: 953 865 298 3753
Fragments arranged by size:
3753 953 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4532 882
Size: 617 3033 2219
Fragments arranged by size:
3033 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2440 2833 2887 3660 493
Size: 711 1236 393 54 773 2702
Fragments arranged by size:
2702 1236 773 711 393 54
BsaBI GATnn'nnATC
Cuts at: 5863 5863
Size: 5869
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3676 4058 4693 275
Size: 53 83 186 936 2143 382 635 1451
Fragments arranged by size:
2143 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4302 4700 4974 5121 5532 4302
Size: 398 274 147 411 4639
Fragments arranged by size:
4639 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5644
Fragments arranged by size:
5644 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2117 3038 3041 1186
Size: 931 921 3 4014
Fragments arranged by size:
4014 931 921 3
BseYI C'CCAG_C
Cuts at: 2047 2483 2656 5072 5568 2047
Size: 436 173 2416 496 2348
Fragments arranged by size:
2416 2348 496 436 173
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2858 1163
Size: 573 1122 4174
Fragments arranged by size:
4174 1122 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2325 3133 3319 4080 5108 1540
Size: 275 186 324 808 186 761 1028 2301
Fragments arranged by size:
2301 1028 808 761 324 275 186 186
BsiHKAI G_wGCw'C
Cuts at: 2289 2373 2424 2454 2814 3930 4015 5086 2289
Size: 84 51 30 360 1116 85 1071 3072
Fragments arranged by size:
3072 1116 1071 360 85 84 51 30
BsmI GAATG_Cn'
Cuts at: 3380 3473 3380
Size: 93 5776
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2121 2289 2307 2373 2424 2454 2814
Size: 399 331 168 18 66 51 30 360
Cuts at: 2814 3033 3089 3188 3930 4015 5086 1391
Size: 219 56 99 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 360 331 219 168
99 85 66 56 51 30 18
BspEI T'CCGG_A
Cuts at: 5532 5532
Size: 5869
BspHI T'CATG_A
Cuts at: 3758 5488 3758
Size: 1730 4139
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2268 2610 2706 3224 844
Size: 1424 342 96 518 3489
Fragments arranged by size:
3489 1424 518 342 96
BsrBI CCG'CTC
Cuts at: 1394 1823 3756 1394
Size: 429 1933 3507
Fragments arranged by size:
3507 1933 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4364 4538 65
Size: 1183 3116 174 1396
Fragments arranged by size:
3116 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2475 2569 4513 4700 1700
Size: 110 665 94 1944 187 2869
Fragments arranged by size:
2869 1944 665 187 110 94
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5869
BssHII G'CGCG_C
Cuts at: 1925 2461 2719 2734 1925
Size: 536 258 15 5060
Fragments arranged by size:
5060 536 258 15
BssSI C'ACGA_G
Cuts at: 3929 4941 3929
Size: 1012 4857
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2075 2458 794
Size: 1281 383 4205
Fragments arranged by size:
4205 1281 383
BstBI TT'CG_AA
Cuts at: 4673 4673
Size: 5869
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5869
BstYI r'GATC_y
Cuts at: 1972 3199 3566 3951 3968 5420 5506 5518 5864
Size: 1227 367 385 17 1452 86 12 346
Cuts at: 5864 1972
Size: 1977
Fragments arranged by size:
1977 1452 1227 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2445 2589 2724 513
Size: 552 1380 144 135 3658
Fragments arranged by size:
3658 1380 552 144 135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2530 487
Size: 683 56 1304 3826
Fragments arranged by size:
3826 1304 683 56
BtsI GCAGTG_nn'
Cuts at: 2612 3048 3385 4179 4199 2612
Size: 436 337 794 20 4282
Fragments arranged by size:
4282 794 436 337 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5869
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5834
DraI TTT'AAA
Cuts at: 3173 3173
Size: 5869
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4876 809
Size: 507 3560 1802
Fragments arranged by size:
3560 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2271 2322 2442
Size: 54 1425 50 275 18 441 51 120
Cuts at: 2442 2490 2581 2609 2679 3130 3316 4205 5834
Size: 48 91 28 70 451 186 889 1629
Cuts at: 5834 8
Size: 43
Fragments arranged by size:
1629 1425 889 451 441 275 186 120
91 70 54 51 50 48 43 28
18
EagI C'GGCC_G
Cuts at: 1537 1812 2322 3130 3316 1537
Size: 275 510 808 186 4090
Fragments arranged by size:
4090 808 510 275 186
EarI CTCTTCn'nnn_
Cuts at: 1353 2294 3799 1353
Size: 941 1505 3423
Fragments arranged by size:
3423 1505 941
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2108 2114 2948 4456 4830 4976
Size: 1064 138 519 6 834 1508 374 146
Cuts at: 4976 387
Size: 1280
Fragments arranged by size:
1508 1280 1064 834 519 374 146 138
6
EcoICRI GAG'CTC
Cuts at: 2287 2287
Size: 5869
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3033 3932 4529 5316 5584 1239
Size: 393 1401 899 597 787 268 1524
Fragments arranged by size:
1524 1401 899 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2117 2117
Size: 5869
EcoRI G'AATT_C
Cuts at: 3178 3556 3178
Size: 378 5491
FspI TGC'GCA
Cuts at: 4375 4375
Size: 5869
HaeII r_GCGC'y
Cuts at: 1678 2013 2088 2761 5016 1678
Size: 335 75 673 2255 2531
Fragments arranged by size:
2531 2255 673 335 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4510 4542 4584 4616 1373
Size: 32 135 32 2938 32 42 32 2626
Fragments arranged by size:
2938 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2380 2881 3213 3459 669
Size: 988 281 442 501 332 246 3079
Fragments arranged by size:
3079 988 501 442 332 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5869
HpaI GTT'AAC
Cuts at: 3459 3459
Size: 5869
KpnI G_GTAC'C
Cuts at: 3194 3194
Size: 5869
MfeI C'AATT_G
Cuts at: 3468 3468
Size: 5869
MluI A'CGCG_T
Cuts at: 5745 5745
Size: 5869
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2973 4983 5167 1305
Size: 1668 2010 184 2007
Fragments arranged by size:
2010 2007 1668 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2492 2611 2681 10
Size: 54 1425 343 660 119 70 3198
Fragments arranged by size:
3198 1425 660 343 119 70 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2213 3827 4186 4345 518
Size: 648 1047 1614 359 159 2042
Fragments arranged by size:
2042 1614 1047 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2842 2916 2974 3285 3962 5110 5355 1606
Size: 1236 74 58 311 677 1148 245 2120
Fragments arranged by size:
2120 1236 1148 677 311 245 74 58
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5317
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5869
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5869
NotI GC'GGCC_GC
Cuts at: 3316 3316
Size: 5869
NspI r_CATG'y
Cuts at: 1132 1229 2395 3085 3227 4772 1132
Size: 97 1166 690 142 1545 2229
Fragments arranged by size:
2229 1545 1166 690 142 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5869
PciI A'CATG_T
Cuts at: 2391 4768 2391
Size: 2377 3492
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2590 1108
Size: 1482 4387
PfoI T'CCnGG_A
Cuts at: 3000 3000
Size: 5869
PmeI GTTT'AAAC
Cuts at: 3173 3173
Size: 5869
PmlI CAC'GTG
Cuts at: 2887 2887
Size: 5869
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3938 3970 5451 5483 3938
Size: 32 1481 32 4324
Fragments arranged by size:
4324 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5869
PsiI TTA'TAA
Cuts at: 3439 3439
Size: 5869
PspOMI G'GGCC_C
Cuts at: 2117 2117
Size: 5869
PstI C_TGCA'G
Cuts at: 830 2071 2607 3221 830
Size: 1241 536 614 3478
Fragments arranged by size:
3478 1241 614 536
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5869
PvuII CAG'CTG
Cuts at: 2842 2916 2842
Size: 74 5795
SacI G_AGCT'C
Cuts at: 2289 2289
Size: 5869
SalI G'TCGA_C
Cuts at: 1936 2378 3211 1936
Size: 442 833 4594
Fragments arranged by size:
4594 833 442
SapI GCTCTTCn'nnn_
Cuts at: 2294 2294
Size: 5869
SbfI CC_TGCA'GG
Cuts at: 3221 3221
Size: 5869
ScaI AGT'ACT
Cuts at: 4117 5829 4117
Size: 1712 4157
SfcI C'TryA_G
Cuts at: 826 1045 2067 2603 3217 4352 5033 5224 826
Size: 219 1022 536 614 1135 681 191 1471
Fragments arranged by size:
1471 1135 1022 681 614 536 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2321 2321
Size: 5869
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5869
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5869
SmaI CCC'GGG
Cuts at: 1872 2032 2122 3196 1872
Size: 160 90 1074 4545
Fragments arranged by size:
4545 1074 160 90
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3056 3973 4874 5136 820
Size: 197 926 1113 917 901 262 1553
Fragments arranged by size:
1553 1113 926 917 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3660 493
Size: 3167 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5869
SphI G_CATG'C
Cuts at: 1132 3227 1132
Size: 2095 3774
SspI AAT'ATT
Cuts at: 5 52 3793 5
Size: 47 3741 2081
Fragments arranged by size:
3741 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5869
StyI C'CwwG_G
Cuts at: 513 1065 3308 513
Size: 552 2243 3074
Fragments arranged by size:
3074 2243 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3896 3913 4066 4251 4696 325
Size: 1255 2316 17 153 185 445 1498
Fragments arranged by size:
2316 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4115 5827 96
Size: 274 80 33 51 1443 2138 1712 138
Fragments arranged by size:
2138 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5829 5861 5829
Size: 32 5837
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5869
XbaI T'CTAG_A
Cuts at: 3205 3205
Size: 5869
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2619 2619
Size: 5869
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5869
XmaI C'CCGG_G
Cuts at: 1870 2030 2120 3194 1870
Size: 160 90 1074 4545
Fragments arranged by size:
4545 1074 160 90
XmnI GAAnn'nnTTC
Cuts at: 3998 3998
Size: 5869
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3677 276
Size: 53 83 186 936 2143 2468
Fragments arranged by size:
2468 2143 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PpuMI PspXI PsrI RsrII SacII SanDI
SexAI SfoI SrfI SwaI