Restriction map for
pFN21AE9503
(Circular) (Six-base) MAPSORT of: pFN21AE9503.seq Check: 9302 from: 1 to: 5857
pFN21AE9503 5857 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2829 3667 278
Size: 53 83 186 936 1293 838 2468
Fragments arranged by size:
2468 1293 936 838 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2247 2490 3200 1937
Size: 310 243 710 4594
Fragments arranged by size:
4594 710 310 243
Acc65I G'GTAC_C
Cuts at: 3178 3178
Size: 5857
AclI AA'CG_TT
Cuts at: 1563 3984 4357 1563
Size: 2421 373 3063
Fragments arranged by size:
3063 2421 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2389 2774 3920 5304 5572 1632
Size: 757 385 1146 1384 268 1917
Fragments arranged by size:
1917 1384 1146 757 385 268
AfeI AGC'GCT
Cuts at: 2432 2612 2432
Size: 180 5677
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5660
AflIII A'CryG_T
Cuts at: 1203 4756 5733 1203
Size: 3553 977 1327
Fragments arranged by size:
3553 1327 977
AgeI A'CCGG_T
Cuts at: 4688 4688
Size: 5857
AhdI GACnn_n'nnGTC
Cuts at: 2243 4586 2243
Size: 2343 3514
AleI CACnn'nnGTG
Cuts at: 1166 2444 1166
Size: 1278 4579
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2972 1184
Size: 1788 4069
AlwNI CAG_nnn'CTG
Cuts at: 3600 5172 3600
Size: 1572 4285
ApaI G_GGCC'C
Cuts at: 2149 2329 2801 2851 3006 2149
Size: 180 472 50 155 5000
Fragments arranged by size:
5000 472 180 155 50
ApaLI G'TGCA_C
Cuts at: 3914 5070 3914
Size: 1156 4701
ApoI r'AATT_y
Cuts at: 1493 3166 3392 3544 5653 1493
Size: 1673 226 152 2109 1697
Fragments arranged by size:
2109 1697 1673 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4411 160
Size: 872 3379 1606
Fragments arranged by size:
3379 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5857
AvaI C'yCGr_G
Cuts at: 1870 1943 2033 2098 3005 3021 3182 1870
Size: 73 90 65 907 16 161 4545
Fragments arranged by size:
4545 907 161 90 73 65 16
BamHI G'GATC_C
Cuts at: 2877 3187 3554 2877
Size: 310 367 5180
Fragments arranged by size:
5180 367 310
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2344 3178 4633 618
Size: 325 217 627 557 834 1455 1842
Fragments arranged by size:
1842 1455 834 627 557 325 217
BanII G_rGCy'C
Cuts at: 1391 2149 2329 2801 2851 3006 3126 3176 1391
Size: 758 180 472 50 155 120 50 4072
Fragments arranged by size:
4072 758 472 180 155 120 50 50
BbeI G_GCGC'C
Cuts at: 2348 2348
Size: 5857
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2762 928
Size: 971 863 4023
Fragments arranged by size:
4023 971 863
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4048 4082 1903
Size: 34 2111 34 3678
Fragments arranged by size:
3678 2111 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3749 4959 3749
Size: 1210 4647
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5857
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2303 3032 4468
Size: 107 122 71 631 605 631 729 1436
Cuts at: 4468 136
Size: 1525
Fragments arranged by size:
1525 1436 729 631 631 605 122 107
71
BglII A'GATC_T
Cuts at: 5852 5852
Size: 5857
BlpI GC'TnA_GC
Cuts at: 2960 3104 3274 2960
Size: 144 170 5543
Fragments arranged by size:
5543 170 144
Bme1580I G_kGCm'C
Cuts at: 1790 2067 2149 2329 2801 2851 3006 3918 5074
Size: 277 82 180 472 50 155 912 1156
Cuts at: 5074 1790
Size: 2573
Fragments arranged by size:
2573 1156 912 472 277 180 155 82
50
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5857
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2347 3588 4546
Size: 416 53 83 399 24 930 1241 958
Cuts at: 4546 442
Size: 1753
Fragments arranged by size:
1753 1241 958 930 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5857
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2975 3055 2975
Size: 80 5777
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2210 2569 3124 4517 1239
Size: 971 359 555 1393 2579
Fragments arranged by size:
2579 1393 971 555 359
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3982 4847 5145 3982
Size: 865 298 4694
Fragments arranged by size:
4694 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4520 882
Size: 617 3021 2219
Fragments arranged by size:
3021 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3648 493
Size: 711 2444 2702
Fragments arranged by size:
2702 2444 711
BsaBI GATnn'nnATC
Cuts at: 5851 5851
Size: 5857
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2345 2646 2826 3664
Size: 53 83 186 936 812 301 180 838
Cuts at: 3664 4046 4681 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 838 812 635 382 301 186
180 83 53
BsaWI w'CCGG_w
Cuts at: 3041 4290 4688 4962 5109 5520 3041
Size: 1249 398 274 147 411 3378
Fragments arranged by size:
3378 1249 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2567 2597 3113 3143 1178
Size: 30 165 30 1164 30 516 30 3892
Fragments arranged by size:
3892 1164 516 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5857
BseYI C'CCAG_C
Cuts at: 2241 2530 2548 2722 2793 2989 5060 5556 2241
Size: 289 18 174 71 196 2071 496 2542
Fragments arranged by size:
2542 2071 496 289 196 174 71 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2069 2714 2968 1163
Size: 573 333 645 254 4052
Fragments arranged by size:
4052 645 573 333 254
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2058 2234 2453 3307 4068 5096
Size: 275 186 57 176 219 854 761 1028
Cuts at: 5096 1540
Size: 2301
Fragments arranged by size:
2301 1028 854 761 275 219 186 176
57
BsiHKAI G_wGCw'C
Cuts at: 2961 3918 4003 5074 2961
Size: 957 85 1071 3744
Fragments arranged by size:
3744 1071 957 85
BsmI GAATG_Cn'
Cuts at: 3368 3461 3368
Size: 93 5764
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 2149 2329 2801 2851 2961 3006
Size: 399 277 82 180 472 50 110 45
Cuts at: 3006 3126 3176 3918 4003 5074 1391
Size: 120 50 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 472 399 277 180 120
110 85 82 50 50 45
BspEI T'CCGG_A
Cuts at: 3041 5520 3041
Size: 2479 3378
BspHI T'CATG_A
Cuts at: 3746 5476 3746
Size: 1730 4127
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2289 2868 3212 844
Size: 1445 579 344 3489
Fragments arranged by size:
3489 1445 579 344
BsrBI CCG'CTC
Cuts at: 1394 1823 2466 3099 3744 1394
Size: 429 643 633 645 3507
Fragments arranged by size:
3507 645 643 633 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4352 4526 65
Size: 1183 3104 174 1396
Fragments arranged by size:
3104 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2057 2070 2103 2452 2642 4501 4688
Size: 110 247 13 33 349 190 1859 187
Cuts at: 4688 1700
Size: 2869
Fragments arranged by size:
2869 1859 349 247 190 187 110 33
13
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5857
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5857
BssSI C'ACGA_G
Cuts at: 3917 4929 3917
Size: 1012 4845
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5857
BstBI TT'CG_AA
Cuts at: 4661 4661
Size: 5857
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5857
BstXI CCAn_nnnn'nTGG
Cuts at: 2179 2662 2179
Size: 483 5374
BstYI r'GATC_y
Cuts at: 1972 2877 3187 3554 3939 3956 5408 5494 5506
Size: 905 310 367 385 17 1452 86 12
Cuts at: 5506 5852 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 905 385 367 346 310 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2013 2442 513
Size: 552 948 429 3928
Fragments arranged by size:
3928 948 552 429
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5118
Fragments arranged by size:
5118 683 56
BtsI GCAGTG_nn'
Cuts at: 2418 3373 4167 4187 2418
Size: 955 794 20 4088
Fragments arranged by size:
4088 955 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5857
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5822
DraI TTT'AAA
Cuts at: 3161 3161
Size: 5857
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4864 809
Size: 507 3548 1802
Fragments arranged by size:
3548 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2109 2166
Size: 54 1425 50 275 18 225 54 57
Cuts at: 2166 2211 2304 2391 2450 2457 2520 2652 2820
Size: 45 93 87 59 7 63 132 168
Cuts at: 2820 2889 3304 4193 5822 8
Size: 69 415 889 1629 43
Fragments arranged by size:
1629 1425 889 415 275 225 168 132
93 87 69 63 59 57 54 54
50 45 43 18 7
EagI C'GGCC_G
Cuts at: 1537 1812 2055 2450 3304 1537
Size: 275 243 395 854 4090
Fragments arranged by size:
4090 854 395 275 243
EarI CTCTTCn'nnn_
Cuts at: 1353 2384 2420 2627 2810 3787 1353
Size: 1031 36 207 183 977 3423
Fragments arranged by size:
3423 1031 977 207 183 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2675 4444 4818 4964 387
Size: 1064 138 1086 1769 374 146 1280
Fragments arranged by size:
1769 1280 1086 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2210 2389 2569 2774 3124 3920 4517
Size: 393 578 179 180 205 350 796 597
Cuts at: 4517 5304 5572 1239
Size: 787 268 1524
Fragments arranged by size:
1524 796 787 597 578 393 350 268
205 180 179
EcoNI CCTnn'n_nnAGG
Cuts at: 2366 2693 2366
Size: 327 5530
EcoO109I rG'GnC_Cy
Cuts at: 2019 2145 2146 2325 2371 2713 2797 3002 2019
Size: 126 1 179 46 342 84 205 4874
Fragments arranged by size:
4874 342 205 179 126 84 46 1
EcoRI G'AATT_C
Cuts at: 3166 3544 3166
Size: 378 5479
FspI TGC'GCA
Cuts at: 4363 4363
Size: 5857
HaeII r_GCGC'y
Cuts at: 1678 2348 2434 2614 5004 1678
Size: 670 86 180 2390 2531
Fragments arranged by size:
2531 2390 670 180 86
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2229 2261 2567 2599 3113
Size: 32 135 32 657 32 306 32 514
Cuts at: 3113 3145 4498 4530 4572 4604 1373
Size: 32 1353 32 42 32 2626
Fragments arranged by size:
2626 1353 657 514 306 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2911 3201 3447 669
Size: 988 281 973 290 246 3079
Fragments arranged by size:
3079 988 973 290 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5857
HpaI GTT'AAC
Cuts at: 3447 3447
Size: 5857
KasI G'GCGC_C
Cuts at: 2344 2344
Size: 5857
KpnI G_GTAC'C
Cuts at: 3182 3182
Size: 5857
MfeI C'AATT_G
Cuts at: 3456 3456
Size: 5857
MluI A'CGCG_T
Cuts at: 5733 5733
Size: 5857
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2862 4971 5155 1305
Size: 1557 2109 184 2007
Fragments arranged by size:
2109 2007 1557 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2306 2654 10
Size: 54 1425 343 474 348 3213
Fragments arranged by size:
3213 1425 474 348 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2444 2660 3815 4174 4333 518
Size: 648 1278 216 1155 359 159 2042
Fragments arranged by size:
2042 1278 1155 648 359 216 159
MspA1I CmG'CkG
Cuts at: 1606 2050 2175 2580 2703 2726 3273 3950 5098
Size: 444 125 405 123 23 547 677 1148
Cuts at: 5098 5343 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 547 444 405 245 125
123 23
NaeI GCC'GGC
Cuts at: 2059 2072 2105 2644 2059
Size: 13 33 539 5272
Fragments arranged by size:
5272 539 33 13
NarI GG'CG_CC
Cuts at: 2345 2345
Size: 5857
NcoI C'CATG_G
Cuts at: 513 1065 2442 513
Size: 552 1377 3928
Fragments arranged by size:
3928 1377 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5857
NgoMIV G'CCGG_C
Cuts at: 2057 2070 2103 2642 2057
Size: 13 33 539 5272
Fragments arranged by size:
5272 539 33 13
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5857
NotI GC'GGCC_GC
Cuts at: 3304 3304
Size: 5857
NruI TCG'CGA
Cuts at: 2236 2236
Size: 5857
NspI r_CATG'y
Cuts at: 1132 1229 2230 2998 3215 4760 1132
Size: 97 1001 768 217 1545 2229
Fragments arranged by size:
2229 1545 1001 768 217 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5857
PciI A'CATG_T
Cuts at: 4756 4756
Size: 5857
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2050 2844 1108
Size: 942 794 4121
Fragments arranged by size:
4121 942 794
PmeI GTTT'AAAC
Cuts at: 3161 3161
Size: 5857
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3926 3958 5439 5471 3926
Size: 32 1481 32 4312
Fragments arranged by size:
4312 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2019 2019
Size: 5857
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5857
PsiI TTA'TAA
Cuts at: 3427 3427
Size: 5857
PspOMI G'GGCC_C
Cuts at: 2145 2325 2797 2847 3002 2145
Size: 180 472 50 155 5000
Fragments arranged by size:
5000 472 180 155 50
PstI C_TGCA'G
Cuts at: 830 2425 3209 830
Size: 1595 784 3478
Fragments arranged by size:
3478 1595 784
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5857
PvuII CAG'CTG
Cuts at: 2050 2175 2050
Size: 125 5732
SalI G'TCGA_C
Cuts at: 1936 3199 1936
Size: 1263 4594
SanDI GG'GwC_CC
Cuts at: 2019 2019
Size: 5857
SapI GCTCTTCn'nnn_
Cuts at: 2384 2420 2384
Size: 36 5821
SbfI CC_TGCA'GG
Cuts at: 3209 3209
Size: 5857
ScaI AGT'ACT
Cuts at: 4105 5817 4105
Size: 1712 4145
SfcI C'TryA_G
Cuts at: 826 1045 2421 3205 4340 5021 5212 826
Size: 219 1376 784 1135 681 191 1471
Fragments arranged by size:
1471 1376 1135 784 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2303 2303
Size: 5857
SfoI GGC'GCC
Cuts at: 2346 2346
Size: 5857
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5857
SgrAI Cr'CCGG_yG
Cuts at: 1700 2642 1700
Size: 942 4915
SmaI CCC'GGG
Cuts at: 1872 3007 3023 3184 1872
Size: 1135 16 161 4545
Fragments arranged by size:
4545 1135 161 16
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3961 4862 5124 820
Size: 197 926 2018 901 262 1553
Fragments arranged by size:
2018 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3648 493
Size: 3155 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5857
SphI G_CATG'C
Cuts at: 1132 2998 3215 1132
Size: 1866 217 3774
Fragments arranged by size:
3774 1866 217
SrfI GCCC'GGGC
Cuts at: 3023 3023
Size: 5857
SspI AAT'ATT
Cuts at: 5 52 3781 5
Size: 47 3729 2081
Fragments arranged by size:
3729 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5857
StyI C'CwwG_G
Cuts at: 513 1065 2442 3074 3296 513
Size: 552 1377 632 222 3074
Fragments arranged by size:
3074 1377 632 552 222
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3884 3901 4054 4239 4684 325
Size: 1255 2304 17 153 185 445 1498
Fragments arranged by size:
2304 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4103 5815 96
Size: 274 80 33 51 1443 2126 1712 138
Fragments arranged by size:
2126 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5817 5849 5817
Size: 32 5825
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5857
XbaI T'CTAG_A
Cuts at: 3193 3193
Size: 5857
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2179 2686 2179
Size: 507 5350
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5857
XmaI C'CCGG_G
Cuts at: 1870 3005 3021 3182 1870
Size: 1135 16 161 4545
Fragments arranged by size:
4545 1135 161 16
XmnI GAAnn'nnTTC
Cuts at: 3986 3986
Size: 5857
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2827 3665 276
Size: 53 83 186 936 1293 838 2468
Fragments arranged by size:
2468 1293 936 838 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI NsiI PacI PfoI PmlI PspXI PsrI
RsrII SacI SacII SexAI SwaI