Restriction map for
pFN21AE9501
(Circular) (Six-base) MAPSORT of: pFN21AE9501.seq Check: 2520 from: 1 to: 6166
pFN21AE9501 6166 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3976 278
Size: 53 83 186 936 2440 2468
Fragments arranged by size:
2468 2440 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2895 3509 1937
Size: 958 614 4594
Fragments arranged by size:
4594 958 614
Acc65I G'GTAC_C
Cuts at: 2406 3487 2406
Size: 1081 5085
AclI AA'CG_TT
Cuts at: 1563 4293 4666 1563
Size: 2730 373 3063
Fragments arranged by size:
3063 2730 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2854 4229 5613 5881 1632
Size: 1222 1375 1384 268 1917
Fragments arranged by size:
1917 1384 1375 1222 268
AflII C'TTAA_G
Cuts at: 820 1017 2411 820
Size: 197 1394 4575
Fragments arranged by size:
4575 1394 197
AflIII A'CryG_T
Cuts at: 1203 3354 5065 6042 1203
Size: 2151 1711 977 1327
Fragments arranged by size:
2151 1711 1327 977
AgeI A'CCGG_T
Cuts at: 4997 4997
Size: 6166
AhdI GACnn_n'nnGTC
Cuts at: 4895 4895
Size: 6166
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6166
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6166
AlwNI CAG_nnn'CTG
Cuts at: 3016 3909 5481 3016
Size: 893 1572 3701
Fragments arranged by size:
3701 1572 893
ApaLI G'TGCA_C
Cuts at: 2927 4223 5379 2927
Size: 1296 1156 3714
Fragments arranged by size:
3714 1296 1156
ApoI r'AATT_y
Cuts at: 1493 3447 3475 3701 3853 5962 1493
Size: 1954 28 226 152 2109 1697
Fragments arranged by size:
2109 1954 1697 226 152 28
AseI AT'TA_AT
Cuts at: 160 1032 4720 160
Size: 872 3688 1606
Fragments arranged by size:
3688 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6166
AvaI C'yCGr_G
Cuts at: 1870 1943 2430 3491 1870
Size: 73 487 1061 4545
Fragments arranged by size:
4545 1061 487 73
BamHI G'GATC_C
Cuts at: 3288 3496 3863 3288
Size: 208 367 5591
Fragments arranged by size:
5591 367 208
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2234 2406 3487 4942 618
Size: 325 217 627 447 172 1081 1455 1842
Fragments arranged by size:
1842 1455 1081 627 447 325 217 172
BanII G_rGCy'C
Cuts at: 1391 2136 2682 3485 1391
Size: 745 546 803 4072
Fragments arranged by size:
4072 803 745 546
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2043 2350 2860 3440 928
Size: 971 144 307 510 580 3654
Fragments arranged by size:
3654 971 580 510 307 144
BbvCI CC'TCA_GC
Cuts at: 2720 2720
Size: 6166
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2646 2680 4357 4391 1903
Size: 34 709 34 1677 34 3678
Fragments arranged by size:
3678 1677 709 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4058 5268 4058
Size: 1210 4956
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6166
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4777 136
Size: 107 122 71 631 605 3105 1525
Fragments arranged by size:
3105 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2640 6161 2640
Size: 3521 2645
BlpI GC'TnA_GC
Cuts at: 3583 3583
Size: 6166
Bme1580I G_kGCm'C
Cuts at: 1790 2931 4227 5383 1790
Size: 1141 1296 1156 2573
Fragments arranged by size:
2573 1296 1156 1141
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6166
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2297 2855 3897
Size: 416 53 83 399 24 880 558 1042
Cuts at: 3897 4855 442
Size: 958 1753
Fragments arranged by size:
1753 1042 958 880 558 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6166
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4826 1239
Size: 3587 2579
Bpu10I CC'TnA_GC
Cuts at: 2105 2720 2105
Size: 615 5551
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2981 4291 5156 5454 2981
Size: 1310 865 298 3693
Fragments arranged by size:
3693 1310 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4829 882
Size: 617 3330 2219
Fragments arranged by size:
3330 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3957 493
Size: 711 2753 2702
Fragments arranged by size:
2753 2702 711
BsaBI GATnn'nnATC
Cuts at: 6160 6160
Size: 6166
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3973 4355 4990 275
Size: 53 83 186 936 2440 382 635 1451
Fragments arranged by size:
2440 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2979 4599 4997 5271 5418 5829 2979
Size: 1620 398 274 147 411 3316
Fragments arranged by size:
3316 1620 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3368 3398 1178
Size: 30 165 30 1965 30 3946
Fragments arranged by size:
3946 1965 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3148 1186
Size: 1962 4204
BseYI C'CCAG_C
Cuts at: 2079 3370 5369 5865 2079
Size: 1291 1999 496 2380
Fragments arranged by size:
2380 1999 1291 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5593
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3616 4377 5405 1540
Size: 275 186 1615 761 1028 2301
Fragments arranged by size:
2301 1615 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2111 2682 2931 4227 4312 5383 2111
Size: 571 249 1296 85 1071 2894
Fragments arranged by size:
2894 1296 1071 571 249 85
BsiWI C'GTAC_G
Cuts at: 2158 2653 2158
Size: 495 5671
BsmI GAATG_Cn'
Cuts at: 3193 3677 3770 3193
Size: 484 93 5589
Fragments arranged by size:
5589 484 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2111 2136 2682 2931 3485 4227 4312
Size: 399 321 25 546 249 554 742 85
Cuts at: 4312 5383 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 554 546 399 321 249
85 25
BspEI T'CCGG_A
Cuts at: 5829 5829
Size: 6166
BspHI T'CATG_A
Cuts at: 2580 4055 5785 2580
Size: 1475 1730 2961
Fragments arranged by size:
2961 1730 1475
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2148 2274 2613 3521 844
Size: 1304 126 339 908 3489
Fragments arranged by size:
3489 1304 908 339 126
BsrBI CCG'CTC
Cuts at: 1394 1823 2170 2385 3189 4053 1394
Size: 429 347 215 804 864 3507
Fragments arranged by size:
3507 864 804 429 347 215
BsrDI GCAATG_nn'
Cuts at: 65 1248 3315 3346 3424 4661 4835 65
Size: 1183 2067 31 78 1237 174 1396
Fragments arranged by size:
2067 1396 1237 1183 174 78 31
BsrFI r'CCGG_y
Cuts at: 1700 1810 4810 4997 1700
Size: 110 3000 187 2869
Fragments arranged by size:
3000 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2196 96
Size: 2100 4066
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6166
BssSI C'ACGA_G
Cuts at: 4226 5238 4226
Size: 1012 5154
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2115 794
Size: 1321 4845
BstBI TT'CG_AA
Cuts at: 4970 4970
Size: 6166
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6166
BstXI CCAn_nnnn'nTGG
Cuts at: 2449 3253 3370 2449
Size: 804 117 5245
Fragments arranged by size:
5245 804 117
BstYI r'GATC_y
Cuts at: 1972 2640 2813 3288 3496 3863 4248 4265 5717
Size: 668 173 475 208 367 385 17 1452
Cuts at: 5717 5803 5815 6161 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 668 475 385 367 346 208
173 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2402 2402
Size: 6166
BtgI C'CryG_G
Cuts at: 513 1065 2319 2986 513
Size: 552 1254 667 3693
Fragments arranged by size:
3693 1254 667 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5427
Fragments arranged by size:
5427 683 56
BtsI GCAGTG_nn'
Cuts at: 2662 3682 4476 4496 2662
Size: 1020 794 20 4332
Fragments arranged by size:
4332 1020 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6166
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6131
DraI TTT'AAA
Cuts at: 3470 3470
Size: 6166
DraIII CAC_nnn'GTG
Cuts at: 2185 2185
Size: 6166
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5173 809
Size: 507 3857 1802
Fragments arranged by size:
3857 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2070 2331 2439
Size: 54 1425 50 275 18 240 261 108
Cuts at: 2439 2989 3613 4502 6131 8
Size: 550 624 889 1629 43
Fragments arranged by size:
1629 1425 889 624 550 275 261 240
108 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3613 1537
Size: 275 1801 4090
Fragments arranged by size:
4090 1801 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3173 4096 1353
Size: 1820 923 3423
Fragments arranged by size:
3423 1820 923
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2234 4753 5127 5273 387
Size: 1064 138 645 2519 374 146 1280
Fragments arranged by size:
2519 1280 1064 645 374 146 138
EcoICRI GAG'CTC
Cuts at: 2680 2680
Size: 6166
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2854 4229 4826 5613 5881 1239
Size: 393 1222 1375 597 787 268 1524
Fragments arranged by size:
1524 1375 1222 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2454 2691 3013 2454
Size: 237 322 5607
Fragments arranged by size:
5607 322 237
EcoRI G'AATT_C
Cuts at: 3447 3475 3853 3447
Size: 28 378 5760
Fragments arranged by size:
5760 378 28
EcoRV GAT'ATC
Cuts at: 3042 3042
Size: 6166
FspI TGC'GCA
Cuts at: 4672 4672
Size: 6166
HaeII r_GCGC'y
Cuts at: 1678 5313 1678
Size: 3635 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2840 2872 3056 3088 4807
Size: 32 135 32 1268 32 184 32 1719
Cuts at: 4807 4839 4881 4913 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1719 1268 184 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2977 3510 3756 669
Size: 988 281 1039 533 246 3079
Fragments arranged by size:
3079 1039 988 533 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6166
HpaI GTT'AAC
Cuts at: 3756 3756
Size: 6166
KpnI G_GTAC'C
Cuts at: 2410 3491 2410
Size: 1081 5085
MfeI C'AATT_G
Cuts at: 3765 3765
Size: 6166
MluI A'CGCG_T
Cuts at: 6042 6042
Size: 6166
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5280 5464 1305
Size: 3975 184 2007
Fragments arranged by size:
3975 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2072 2333 2991 10
Size: 54 1425 343 240 261 658 3185
Fragments arranged by size:
3185 1425 658 343 261 240 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2249 4124 4483 4642 518
Size: 648 1083 1875 359 159 2042
Fragments arranged by size:
2042 1875 1083 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2083 3582 4259 5407 5652 1606
Size: 477 1499 677 1148 245 2120
Fragments arranged by size:
2120 1499 1148 677 477 245
NcoI C'CATG_G
Cuts at: 513 1065 2319 2986 513
Size: 552 1254 667 3693
Fragments arranged by size:
3693 1254 667 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6166
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6166
NotI GC'GGCC_GC
Cuts at: 3613 3613
Size: 6166
NspI r_CATG'y
Cuts at: 1132 1229 2935 3358 3524 5069 1132
Size: 97 1706 423 166 1545 2229
Fragments arranged by size:
2229 1706 1545 423 166 97
PasI CC'CwG_GG
Cuts at: 1348 2772 1348
Size: 1424 4742
PciI A'CATG_T
Cuts at: 3354 5065 3354
Size: 1711 4455
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2185 1108
Size: 1077 5089
PmeI GTTT'AAAC
Cuts at: 3470 3470
Size: 6166
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4235 4267 5748 5780 4235
Size: 32 1481 32 4621
Fragments arranged by size:
4621 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2691 2691
Size: 6166
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6166
PsiI TTA'TAA
Cuts at: 3736 3736
Size: 6166
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2246 2278 2246
Size: 32 6134
PstI C_TGCA'G
Cuts at: 830 3518 830
Size: 2688 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6166
PvuII CAG'CTG
Cuts at: 2083 2083
Size: 6166
SacI G_AGCT'C
Cuts at: 2682 2682
Size: 6166
SalI G'TCGA_C
Cuts at: 1936 3508 1936
Size: 1572 4594
SbfI CC_TGCA'GG
Cuts at: 3518 3518
Size: 6166
ScaI AGT'ACT
Cuts at: 4414 6126 4414
Size: 1712 4454
SexAI A'CCwGG_T
Cuts at: 2567 2567
Size: 6166
SfcI C'TryA_G
Cuts at: 826 1045 3514 4649 5330 5521 826
Size: 219 2469 1135 681 191 1471
Fragments arranged by size:
2469 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6166
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6166
SmaI CCC'GGG
Cuts at: 1872 3493 1872
Size: 1621 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2411 2996 4270 5171 5433 820
Size: 197 926 468 585 1274 901 262 1553
Fragments arranged by size:
1553 1274 926 901 585 468 262 197
SnaBI TAC'GTA
Cuts at: 493 3957 493
Size: 3464 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6166
SphI G_CATG'C
Cuts at: 1132 3524 1132
Size: 2392 3774
SspI AAT'ATT
Cuts at: 5 52 4090 5
Size: 47 4038 2081
Fragments arranged by size:
4038 2081 47
StuI AGG'CCT
Cuts at: 1517 3153 1517
Size: 1636 4530
StyI C'CwwG_G
Cuts at: 513 1065 2319 2736 2844 2986 3605 513
Size: 552 1254 417 108 142 619 3074
Fragments arranged by size:
3074 1254 619 552 417 142 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2222 4193 4210 4363 4548 4993 325
Size: 1255 642 1971 17 153 185 445 1498
Fragments arranged by size:
1971 1498 1255 642 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2196 4412 6124
Size: 274 80 33 51 1443 219 2216 1712
Cuts at: 6124 96
Size: 138
Fragments arranged by size:
2216 1712 1443 274 219 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6126 6158 6126
Size: 32 6134
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6166
XbaI T'CTAG_A
Cuts at: 3502 3502
Size: 6166
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2284 2326 2284
Size: 42 6124
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6166
XmaI C'CCGG_G
Cuts at: 1870 3491 1870
Size: 1621 4545
XmnI GAAnn'nnTTC
Cuts at: 2504 3437 4295 2504
Size: 933 858 4375
Fragments arranged by size:
4375 933 858
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3974 276
Size: 53 83 186 936 2440 2468
Fragments arranged by size:
2468 2440 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsmBI BstZ17I EcoNI FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PspOMI PspXI RsrII SacII SanDI SapI SfiI
SfoI SrfI SwaI