Restriction map for
pFN21AE9461
(Circular) (Six-base) MAPSORT of: pFN21AE9461.seq Check: 5716 from: 1 to: 6148
pFN21AE9461 6148 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2993 2993
Size: 6148
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3958 278
Size: 53 83 186 936 2422 2468
Fragments arranged by size:
2468 2422 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3105 3491 1937
Size: 1168 386 4594
Fragments arranged by size:
4594 1168 386
Acc65I G'GTAC_C
Cuts at: 3469 3469
Size: 6148
AclI AA'CG_TT
Cuts at: 1563 4275 4648 1563
Size: 2712 373 3063
Fragments arranged by size:
3063 2712 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2507 4211 5595 5863 1632
Size: 875 1704 1384 268 1917
Fragments arranged by size:
1917 1704 1384 875 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5951
AflIII A'CryG_T
Cuts at: 1203 5047 6024 1203
Size: 3844 977 1327
Fragments arranged by size:
3844 1327 977
AgeI A'CCGG_T
Cuts at: 4979 4979
Size: 6148
AhdI GACnn_n'nnGTC
Cuts at: 4877 4877
Size: 6148
AleI CACnn'nnGTG
Cuts at: 1166 3086 3242 1166
Size: 1920 156 4072
Fragments arranged by size:
4072 1920 156
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6148
AlwNI CAG_nnn'CTG
Cuts at: 2308 2667 3184 3891 5463 2308
Size: 359 517 707 1572 2993
Fragments arranged by size:
2993 1572 707 517 359
ApaLI G'TGCA_C
Cuts at: 4205 5361 4205
Size: 1156 4992
ApoI r'AATT_y
Cuts at: 1493 3457 3683 3835 5944 1493
Size: 1964 226 152 2109 1697
Fragments arranged by size:
2109 1964 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4702 160
Size: 872 3670 1606
Fragments arranged by size:
3670 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6148
AvaI C'yCGr_G
Cuts at: 1870 1943 2888 3473 1870
Size: 73 945 585 4545
Fragments arranged by size:
4545 945 585 73
BamHI G'GATC_C
Cuts at: 3478 3845 3478
Size: 367 5781
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2078 2144 2285 2329 2736
Size: 325 217 627 291 66 141 44 407
Cuts at: 2736 2846 3230 3469 4924 618
Size: 110 384 239 1455 1842
Fragments arranged by size:
1842 1455 627 407 384 325 291 239
217 141 110 66 44
BanII G_rGCy'C
Cuts at: 1391 2384 2780 2805 3467 1391
Size: 993 396 25 662 4072
Fragments arranged by size:
4072 993 662 396 25
BbeI G_GCGC'C
Cuts at: 3234 3234
Size: 6148
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5177
BbvCI CC'TCA_GC
Cuts at: 2875 2875
Size: 6148
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4339 4373 1903
Size: 34 50 34 2318 34 3678
Fragments arranged by size:
3678 2318 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2376 4040 5250 2376
Size: 1664 1210 3274
Fragments arranged by size:
3274 1664 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6148
BfrBI ATG'CAT
Cuts at: 2862 2862
Size: 6148
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2519 2759 4759
Size: 107 122 71 631 605 847 240 2000
Cuts at: 4759 136
Size: 1525
Fragments arranged by size:
2000 1525 847 631 605 240 122 107
71
BglII A'GATC_T
Cuts at: 6143 6143
Size: 6148
BlpI GC'TnA_GC
Cuts at: 2060 3565 2060
Size: 1505 4643
Bme1580I G_kGCm'C
Cuts at: 1790 2083 2147 2288 2332 2625 2674 2849 3440
Size: 293 64 141 44 293 49 175 591
Cuts at: 3440 4209 5365 1790
Size: 769 1156 2573
Fragments arranged by size:
2573 1156 769 591 293 293 175 141
64 49 44
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6148
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2606 3236 3879
Size: 416 53 83 399 24 1189 630 643
Cuts at: 3879 4837 442
Size: 958 1753
Fragments arranged by size:
1753 1189 958 643 630 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6148
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2656 3400 2656
Size: 744 5404
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3003 4808 1239
Size: 1764 1805 2579
Fragments arranged by size:
2579 1805 1764
Bpu10I CC'TnA_GC
Cuts at: 2799 2875 2799
Size: 76 6072
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3353 4273 5138 5436 3353
Size: 920 865 298 4065
Fragments arranged by size:
4065 920 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2074 2105 2641 4811 882
Size: 617 575 31 536 2170 2219
Fragments arranged by size:
2219 2170 617 575 536 31
BsaAI yAC'GTr
Cuts at: 493 1204 2695 3939 493
Size: 711 1491 1244 2702
Fragments arranged by size:
2702 1491 1244 711
BsaBI GATnn'nnATC
Cuts at: 6142 6142
Size: 6148
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3231 3955 4337 4972
Size: 53 83 186 936 1698 724 382 635
Cuts at: 4972 275
Size: 1451
Fragments arranged by size:
1698 1451 936 724 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2368 4581 4979 5253 5400 5811 2368
Size: 2213 398 274 147 411 2705
Fragments arranged by size:
2705 2213 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3197 3227 3408 3438 1178
Size: 30 165 30 1794 30 181 30 3888
Fragments arranged by size:
3888 1794 181 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2171 2204 2629 3329 1186
Size: 985 33 425 700 4005
Fragments arranged by size:
4005 985 700 425 33
BseYI C'CCAG_C
Cuts at: 3271 5351 5847 3271
Size: 2080 496 3572
Fragments arranged by size:
3572 2080 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2072 2192 3058 3100 1163
Size: 573 336 120 866 42 4211
Fragments arranged by size:
4211 866 573 336 120 42
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3598 4359 5387 1540
Size: 275 186 1597 761 1028 2301
Fragments arranged by size:
2301 1597 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2384 2805 4209 4294 5365 2384
Size: 421 1404 85 1071 3167
Fragments arranged by size:
3167 1404 1071 421 85
BsmI GAATG_Cn'
Cuts at: 3659 3752 3659
Size: 93 6055
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2083 2147 2288 2332 2384 2625 2674
Size: 399 293 64 141 44 52 241 49
Cuts at: 2674 2780 2805 2849 3440 3467 4209 4294 5365
Size: 106 25 44 591 27 742 85 1071
Cuts at: 5365 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 591 399 293 241 141
106 85 64 52 49 44 44 27
25
BspEI T'CCGG_A
Cuts at: 5811 5811
Size: 6148
BspHI T'CATG_A
Cuts at: 2865 4037 5767 2865
Size: 1172 1730 3246
Fragments arranged by size:
3246 1730 1172
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2993 3503 844
Size: 2149 510 3489
Fragments arranged by size:
3489 2149 510
BsrBI CCG'CTC
Cuts at: 1394 1823 2734 4035 1394
Size: 429 911 1301 3507
Fragments arranged by size:
3507 1301 911 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4643 4817 65
Size: 1183 3395 174 1396
Fragments arranged by size:
3395 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4792 4979 1700
Size: 110 2982 187 2869
Fragments arranged by size:
2982 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6148
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6148
BssSI C'ACGA_G
Cuts at: 3053 4208 5220 3053
Size: 1155 1012 3981
Fragments arranged by size:
3981 1155 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2182 3444 794
Size: 1388 1262 3498
Fragments arranged by size:
3498 1388 1262
BstBI TT'CG_AA
Cuts at: 4952 4952
Size: 6148
BstEII G'GTnAC_C
Cuts at: 1184 2371 1184
Size: 1187 4961
BstXI CCAn_nnnn'nTGG
Cuts at: 2248 2788 2248
Size: 540 5608
BstYI r'GATC_y
Cuts at: 1972 2349 3478 3845 4230 4247 5699 5785 5797
Size: 377 1129 367 385 17 1452 86 12
Cuts at: 5797 6143 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1129 385 377 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2187 2187
Size: 6148
BtgI C'CryG_G
Cuts at: 513 1065 2841 3084 3099 513
Size: 552 1776 243 15 3562
Fragments arranged by size:
3562 1776 552 243 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5409
Fragments arranged by size:
5409 683 56
BtsI GCAGTG_nn'
Cuts at: 3065 3664 4458 4478 3065
Size: 599 794 20 4735
Fragments arranged by size:
4735 794 599 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6148
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6113
DraI TTT'AAA
Cuts at: 3452 3452
Size: 6148
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3379 5155 809
Size: 507 2063 1776 1802
Fragments arranged by size:
2063 1802 1776 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2253 2697 2751
Size: 54 1425 50 275 18 423 444 54
Cuts at: 2751 3595 4484 6113 8
Size: 844 889 1629 43
Fragments arranged by size:
1629 1425 889 844 444 423 275 54
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3595 1537
Size: 275 1783 4090
Fragments arranged by size:
4090 1783 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2483 2978 3391 4078 1353
Size: 1130 495 413 687 3423
Fragments arranged by size:
3423 1130 687 495 413
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2213 4735 5109 5255 387
Size: 1064 138 624 2522 374 146 1280
Fragments arranged by size:
2522 1280 1064 624 374 146 138
EcoICRI GAG'CTC
Cuts at: 2382 2803 2382
Size: 421 5727
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2507 3003 4211 4808 5595 5863 1239
Size: 393 875 496 1208 597 787 268 1524
Fragments arranged by size:
1524 1208 875 787 597 496 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2714 2714
Size: 6148
EcoO109I rG'GnC_Cy
Cuts at: 3014 3014
Size: 6148
EcoRI G'AATT_C
Cuts at: 3457 3835 3457
Size: 378 5770
FspI TGC'GCA
Cuts at: 4654 4654
Size: 6148
HaeII r_GCGC'y
Cuts at: 1678 2417 2496 3234 5295 1678
Size: 739 79 738 2061 2531
Fragments arranged by size:
2531 2061 739 738 79
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2302 2334 4789 4821 4863
Size: 32 135 32 730 32 2455 32 42
Cuts at: 4863 4895 1373
Size: 32 2626
Fragments arranged by size:
2626 2455 730 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3492 3738 669
Size: 988 281 1554 246 3079
Fragments arranged by size:
3079 1554 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6148
HpaI GTT'AAC
Cuts at: 3738 3738
Size: 6148
KasI G'GCGC_C
Cuts at: 3230 3230
Size: 6148
KpnI G_GTAC'C
Cuts at: 3473 3473
Size: 6148
MfeI C'AATT_G
Cuts at: 3747 3747
Size: 6148
MluI A'CGCG_T
Cuts at: 6024 6024
Size: 6148
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3336 5262 5446 1305
Size: 2031 1926 184 2007
Fragments arranged by size:
2031 2007 1926 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2255 2699 2753 10
Size: 54 1425 343 423 444 54 3405
Fragments arranged by size:
3405 1425 444 423 343 54 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2771 3086 3146 3242 4106 4465 4624
Size: 648 1605 315 60 96 864 359 159
Cuts at: 4624 518
Size: 2042
Fragments arranged by size:
2042 1605 864 648 359 315 159 96
60
MspA1I CmG'CkG
Cuts at: 1606 2818 2992 3271 3564 4241 5389 5634 1606
Size: 1212 174 279 293 677 1148 245 2120
Fragments arranged by size:
2120 1212 1148 677 293 279 245 174
NarI GG'CG_CC
Cuts at: 3231 3231
Size: 6148
NcoI C'CATG_G
Cuts at: 513 1065 2841 513
Size: 552 1776 3820
Fragments arranged by size:
3820 1776 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6148
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6148
NotI GC'GGCC_GC
Cuts at: 3595 3595
Size: 6148
NsiI A_TGCA'T
Cuts at: 2864 2864
Size: 6148
NspI r_CATG'y
Cuts at: 1132 1229 2935 3506 5051 1132
Size: 97 1706 571 1545 2229
Fragments arranged by size:
2229 1706 1545 571 97
PasI CC'CwG_GG
Cuts at: 1348 2674 3408 3433 1348
Size: 1326 734 25 4063
Fragments arranged by size:
4063 1326 734 25
PciI A'CATG_T
Cuts at: 5047 5047
Size: 6148
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2297 3184 1108
Size: 902 287 887 4072
Fragments arranged by size:
4072 902 887 287
PfoI T'CCnGG_A
Cuts at: 3285 3285
Size: 6148
PmeI GTTT'AAAC
Cuts at: 3452 3452
Size: 6148
PmlI CAC'GTG
Cuts at: 2695 2695
Size: 6148
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4217 4249 5730 5762 4217
Size: 32 1481 32 4603
Fragments arranged by size:
4603 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3101 1613
Size: 1488 4660
PsiI TTA'TAA
Cuts at: 3718 3718
Size: 6148
PstI C_TGCA'G
Cuts at: 830 2235 3266 3500 830
Size: 1405 1031 234 3478
Fragments arranged by size:
3478 1405 1031 234
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6148
PvuII CAG'CTG
Cuts at: 2818 2992 2818
Size: 174 5974
SacI G_AGCT'C
Cuts at: 2384 2805 2384
Size: 421 5727
SalI G'TCGA_C
Cuts at: 1936 3490 1936
Size: 1554 4594
SapI GCTCTTCn'nnn_
Cuts at: 2483 2483
Size: 6148
SbfI CC_TGCA'GG
Cuts at: 3500 3500
Size: 6148
ScaI AGT'ACT
Cuts at: 4396 6108 4396
Size: 1712 4436
SfcI C'TryA_G
Cuts at: 826 1045 2231 2345 2386 3262 3496 4631 5312
Size: 219 1186 114 41 876 234 1135 681
Cuts at: 5312 5503 826
Size: 191 1471
Fragments arranged by size:
1471 1186 1135 876 681 234 219 191
114 41
SfoI GGC'GCC
Cuts at: 3232 3232
Size: 6148
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6148
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6148
SmaI CCC'GGG
Cuts at: 1872 3475 1872
Size: 1603 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3368 4252 5153 5415 820
Size: 197 926 1425 884 901 262 1553
Fragments arranged by size:
1553 1425 926 901 884 262 197
SnaBI TAC'GTA
Cuts at: 493 3939 493
Size: 3446 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6148
SphI G_CATG'C
Cuts at: 1132 3506 1132
Size: 2374 3774
SspI AAT'ATT
Cuts at: 5 52 4072 5
Size: 47 4020 2081
Fragments arranged by size:
4020 2081 47
StuI AGG'CCT
Cuts at: 1517 3281 3338 1517
Size: 1764 57 4327
Fragments arranged by size:
4327 1764 57
StyI C'CwwG_G
Cuts at: 513 1065 2163 2247 2715 2841 3587 513
Size: 552 1098 84 468 126 746 3074
Fragments arranged by size:
3074 1098 746 552 468 126 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2390 2443 2588 2761 3389 4175 4192
Size: 1255 810 53 145 173 628 786 17
Cuts at: 4192 4345 4530 4975 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 810 786 628 445 185 173
153 145 53 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4394 6106 96
Size: 274 80 33 51 1443 2417 1712 138
Fragments arranged by size:
2417 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3408 3440 6108 6140 3408
Size: 32 2668 32 3416
Fragments arranged by size:
3416 2668 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6148
XbaI T'CTAG_A
Cuts at: 3484 3484
Size: 6148
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2248 2407 2848 2248
Size: 159 441 5548
Fragments arranged by size:
5548 441 159
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6148
XmaI C'CCGG_G
Cuts at: 1870 3473 1870
Size: 1603 4545
XmnI GAAnn'nnTTC
Cuts at: 4277 4277
Size: 6148
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3956 276
Size: 53 83 186 936 2422 2468
Fragments arranged by size:
2468 2422 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BsiWI BsmBI
BstZ17I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV
NruI PacI PpuMI PspOMI PspXI PsrI RsrII SacII
SanDI SexAI SfiI SrfI SwaI