Restriction map for
pFN21AE9459
(Circular) (Six-base) MAPSORT of: pFN21AE9459.seq Check: 6445 from: 1 to: 6292
pFN21AE9459 6292 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3131 3131
Size: 6292
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4102 278
Size: 53 83 186 936 2566 2468
Fragments arranged by size:
2566 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2451 2784 3635 1937
Size: 514 333 851 4594
Fragments arranged by size:
4594 851 514 333
Acc65I G'GTAC_C
Cuts at: 3613 3613
Size: 6292
AclI AA'CG_TT
Cuts at: 1563 4419 4792 1563
Size: 2856 373 3063
Fragments arranged by size:
3063 2856 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2188 2733 3064 3172 3384 4355 5739 6007
Size: 556 545 331 108 212 971 1384 268
Cuts at: 6007 1632
Size: 1917
Fragments arranged by size:
1917 1384 971 556 545 331 268 212
108
AfeI AGC'GCT
Cuts at: 2953 2953
Size: 6292
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6095
AflIII A'CryG_T
Cuts at: 1203 3237 5191 6168 1203
Size: 2034 1954 977 1327
Fragments arranged by size:
2034 1954 1327 977
AgeI A'CCGG_T
Cuts at: 3030 5123 3030
Size: 2093 4199
AhdI GACnn_n'nnGTC
Cuts at: 3238 5021 3238
Size: 1783 4509
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6292
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6292
AlwNI CAG_nnn'CTG
Cuts at: 3154 3541 4035 5607 3154
Size: 387 494 1572 3839
Fragments arranged by size:
3839 1572 494 387
ApaI G_GGCC'C
Cuts at: 2858 2858
Size: 6292
ApaLI G'TGCA_C
Cuts at: 2669 4349 5505 2669
Size: 1680 1156 3456
Fragments arranged by size:
3456 1680 1156
ApoI r'AATT_y
Cuts at: 1493 3601 3827 3979 6088 1493
Size: 2108 226 152 2109 1697
Fragments arranged by size:
2109 2108 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4846 160
Size: 872 3814 1606
Fragments arranged by size:
3814 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6292
AvaI C'yCGr_G
Cuts at: 1870 1943 2357 2911 3487 3617 1870
Size: 73 414 554 576 130 4545
Fragments arranged by size:
4545 576 554 414 130 73
BamHI G'GATC_C
Cuts at: 3622 3989 3622
Size: 367 5925
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2550 2834 3613 5068 618
Size: 325 217 627 763 284 779 1455 1842
Fragments arranged by size:
1842 1455 779 763 627 325 284 217
BanII G_rGCy'C
Cuts at: 1391 2267 2858 3293 3611 1391
Size: 876 591 435 318 4072
Fragments arranged by size:
4072 876 591 435 318
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2194 928
Size: 971 295 5026
Fragments arranged by size:
5026 971 295
BbvCI CC'TCA_GC
Cuts at: 2024 2186 2024
Size: 162 6130
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2179 2213 4483 4517 1903
Size: 34 242 34 2270 34 3678
Fragments arranged by size:
3678 2270 242 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4184 5394 4184
Size: 1210 5082
BclI T'GATC_A
Cuts at: 1548 2331 1548
Size: 783 5509
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2882 3157 3416
Size: 107 122 71 631 605 1210 275 259
Cuts at: 3416 3513 4903 136
Size: 97 1390 1525
Fragments arranged by size:
1525 1390 1210 631 605 275 259 122
107 97 71
BglII A'GATC_T
Cuts at: 2583 6287 2583
Size: 3704 2588
BlpI GC'TnA_GC
Cuts at: 3709 3709
Size: 6292
Bme1580I G_kGCm'C
Cuts at: 1790 2596 2673 2858 3162 4353 5509 1790
Size: 806 77 185 304 1191 1156 2573
Fragments arranged by size:
2573 1191 1156 806 304 185 77
BmgBI CAC'GTC
Cuts at: 1318 3240 1318
Size: 1922 4370
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2086 3020 4023
Size: 416 53 83 399 24 669 934 1003
Cuts at: 4023 4981 442
Size: 958 1753
Fragments arranged by size:
1753 1003 958 934 669 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6292
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2713 2939 4952 1239
Size: 1474 226 2013 2579
Fragments arranged by size:
2579 2013 1474 226
Bpu10I CC'TnA_GC
Cuts at: 2024 2186 2024
Size: 162 6130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3248 4417 5282 5580 3248
Size: 1169 865 298 3960
Fragments arranged by size:
3960 1169 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4955 882
Size: 617 3456 2219
Fragments arranged by size:
3456 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4083 493
Size: 711 2879 2702
Fragments arranged by size:
2879 2702 711
BsaBI GATnn'nnATC
Cuts at: 6286 6286
Size: 6292
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2079 4099 4481 5116
Size: 53 83 186 936 546 2020 382 635
Cuts at: 5116 275
Size: 1451
Fragments arranged by size:
2020 1451 936 635 546 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3030 4725 5123 5397 5544 5955 3030
Size: 1695 398 274 147 411 3367
Fragments arranged by size:
3367 1695 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6067
Fragments arranged by size:
6067 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2008 2072 2075 2168 2309 2711 2734 3461
Size: 822 64 3 93 141 402 23 727
Cuts at: 3461 1186
Size: 4017
Fragments arranged by size:
4017 822 727 402 141 93 64 23
3
BseYI C'CCAG_C
Cuts at: 5495 5991 5495
Size: 496 5796
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3250 1163
Size: 573 1514 4205
Fragments arranged by size:
4205 1514 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2377 3742 4503 5531 1540
Size: 275 186 376 1365 761 1028 2301
Fragments arranged by size:
2301 1365 1028 761 376 275 186
BsiHKAI G_wGCw'C
Cuts at: 2267 2673 3293 4353 4438 5509 2267
Size: 406 620 1060 85 1071 3050
Fragments arranged by size:
3050 1071 1060 620 406 85
BsmI GAATG_Cn'
Cuts at: 2770 3803 3896 2770
Size: 1033 93 5166
Fragments arranged by size:
5166 1033 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2911 2911
Size: 6292
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2267 2596 2673 2858 3162 3293 3611
Size: 399 477 329 77 185 304 131 318
Cuts at: 3611 4353 4438 5509 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 477 399 329 318 304
185 131 85 77
BspEI T'CCGG_A
Cuts at: 5955 5955
Size: 6292
BspHI T'CATG_A
Cuts at: 4181 5911 4181
Size: 1730 4562
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2334 3131 3647 844
Size: 1490 797 516 3489
Fragments arranged by size:
3489 1490 797 516
BsrBI CCG'CTC
Cuts at: 1394 1823 4179 1394
Size: 429 2356 3507
Fragments arranged by size:
3507 2356 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2364 4787 4961 65
Size: 1183 1116 2423 174 1396
Fragments arranged by size:
2423 1396 1183 1116 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3030 4936 5123 1700
Size: 110 1220 1906 187 2869
Fragments arranged by size:
2869 1906 1220 187 110
BsrGI T'GTAC_A
Cuts at: 96 2160 96
Size: 2064 4228
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6292
BssSI C'ACGA_G
Cuts at: 4352 5364 4352
Size: 1012 5280
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6292
BstBI TT'CG_AA
Cuts at: 5096 5096
Size: 6292
BstEII G'GTnAC_C
Cuts at: 1184 2152 2845 1184
Size: 968 693 4631
Fragments arranged by size:
4631 968 693
BstXI CCAn_nnnn'nTGG
Cuts at: 2824 2824
Size: 6292
BstYI r'GATC_y
Cuts at: 1972 2283 2583 3622 3989 4374 4391 5843 5929
Size: 311 300 1039 367 385 17 1452 86
Cuts at: 5929 5941 6287 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1039 385 367 346 311 300
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2400 2508 513
Size: 552 1335 108 4297
Fragments arranged by size:
4297 1335 552 108
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2083 2857 487
Size: 683 56 857 774 3922
Fragments arranged by size:
3922 857 774 683 56
BtsI GCAGTG_nn'
Cuts at: 3126 3262 3808 4602 4622 3126
Size: 136 546 794 20 4796
Fragments arranged by size:
4796 794 546 136 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6292
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6257
DraI TTT'AAA
Cuts at: 3596 3596
Size: 6292
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5299 809
Size: 507 3983 1802
Fragments arranged by size:
3983 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2646 2790 3402
Size: 54 1425 50 275 18 816 144 612
Cuts at: 3402 3739 4628 6257 8
Size: 337 889 1629 43
Fragments arranged by size:
1629 1425 889 816 612 337 275 144
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3739 1537
Size: 275 1927 4090
Fragments arranged by size:
4090 1927 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2603 2752 3083 4222 1353
Size: 1250 149 331 1139 3423
Fragments arranged by size:
3423 1250 1139 331 149
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4879 5253 5399 387
Size: 1064 138 3290 374 146 1280
Fragments arranged by size:
3290 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2265 3291 2265
Size: 1026 5266
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2188 2713 2733 2939 3064 3172 3384
Size: 393 556 525 20 206 125 108 212
Cuts at: 3384 4355 4952 5739 6007 1239
Size: 971 597 787 268 1524
Fragments arranged by size:
1524 971 787 597 556 525 393 268
212 206 125 108 20
EcoO109I rG'GnC_Cy
Cuts at: 2854 2855 2884 2854
Size: 1 29 6262
Fragments arranged by size:
6262 29 1
EcoRI G'AATT_C
Cuts at: 3601 3979 3601
Size: 378 5914
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2218 2218
Size: 6292
FspI TGC'GCA
Cuts at: 4798 4798
Size: 6292
HaeII r_GCGC'y
Cuts at: 1678 2955 5439 1678
Size: 1277 2484 2531
Fragments arranged by size:
2531 2484 1277
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3224 3256 4933 4965 5007
Size: 32 135 32 1652 32 1677 32 42
Cuts at: 5007 5039 1373
Size: 32 2626
Fragments arranged by size:
2626 1677 1652 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3636 3882 669
Size: 988 281 1698 246 3079
Fragments arranged by size:
3079 1698 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3397 748
Size: 2649 3643
HpaI GTT'AAC
Cuts at: 3882 3882
Size: 6292
KpnI G_GTAC'C
Cuts at: 3617 3617
Size: 6292
MfeI C'AATT_G
Cuts at: 3891 3891
Size: 6292
MluI A'CGCG_T
Cuts at: 6168 6168
Size: 6292
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2244 3114 5406 5590 1305
Size: 939 870 2292 184 2007
Fragments arranged by size:
2292 2007 939 870 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2648 3404 10
Size: 54 1425 343 816 756 2898
Fragments arranged by size:
2898 1425 816 756 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2618 2822 4250 4609 4768 518
Size: 648 1452 204 1428 359 159 2042
Fragments arranged by size:
2042 1452 1428 648 359 204 159
MspA1I CmG'CkG
Cuts at: 1606 2028 3192 3708 4385 5533 5778 1606
Size: 422 1164 516 677 1148 245 2120
Fragments arranged by size:
2120 1164 1148 677 516 422 245
NcoI C'CATG_G
Cuts at: 513 1065 2400 513
Size: 552 1335 4405
Fragments arranged by size:
4405 1335 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6292
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6292
NotI GC'GGCC_GC
Cuts at: 3739 3739
Size: 6292
NspI r_CATG'y
Cuts at: 1132 1229 2350 3019 3650 5195 1132
Size: 97 1121 669 631 1545 2229
Fragments arranged by size:
2229 1545 1121 669 631 97
PasI CC'CwG_GG
Cuts at: 1348 3154 1348
Size: 1806 4486
PciI A'CATG_T
Cuts at: 5191 5191
Size: 6292
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6292
PmeI GTTT'AAAC
Cuts at: 3596 3596
Size: 6292
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4361 4393 5874 5906 4361
Size: 32 1481 32 4747
Fragments arranged by size:
4747 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6292
PsiI TTA'TAA
Cuts at: 3862 3862
Size: 6292
PspOMI G'GGCC_C
Cuts at: 2854 2854
Size: 6292
PstI C_TGCA'G
Cuts at: 830 2033 3546 3644 830
Size: 1203 1513 98 3478
Fragments arranged by size:
3478 1513 1203 98
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6292
PvuII CAG'CTG
Cuts at: 2028 3192 2028
Size: 1164 5128
SacI G_AGCT'C
Cuts at: 2267 3293 2267
Size: 1026 5266
SalI G'TCGA_C
Cuts at: 1936 3634 1936
Size: 1698 4594
SapI GCTCTTCn'nnn_
Cuts at: 3083 3083
Size: 6292
SbfI CC_TGCA'GG
Cuts at: 3644 3644
Size: 6292
ScaI AGT'ACT
Cuts at: 4540 6252 4540
Size: 1712 4580
SexAI A'CCwGG_T
Cuts at: 2139 2139
Size: 6292
SfcI C'TryA_G
Cuts at: 826 1045 2029 3542 3640 4775 5456 5647 826
Size: 219 984 1513 98 1135 681 191 1471
Fragments arranged by size:
1513 1471 1135 984 681 219 191 98
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6292
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6292
SmaI CCC'GGG
Cuts at: 1872 3489 3619 1872
Size: 1617 130 4545
Fragments arranged by size:
4545 1617 130
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3227 4396 5297 5559 820
Size: 197 926 1284 1169 901 262 1553
Fragments arranged by size:
1553 1284 1169 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4083 493
Size: 3590 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6292
SphI G_CATG'C
Cuts at: 1132 2350 3650 1132
Size: 1218 1300 3774
Fragments arranged by size:
3774 1300 1218
SspI AAT'ATT
Cuts at: 5 52 4216 5
Size: 47 4164 2081
Fragments arranged by size:
4164 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6292
StyI C'CwwG_G
Cuts at: 513 1065 2400 3378 3731 513
Size: 552 1335 978 353 3074
Fragments arranged by size:
3074 1335 978 552 353
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2105 3292 4319 4336 4489 4674 5119
Size: 1255 525 1187 1027 17 153 185 445
Cuts at: 5119 325
Size: 1498
Fragments arranged by size:
1498 1255 1187 1027 525 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2160 3367 4538
Size: 274 80 33 51 1443 183 1207 1171
Cuts at: 4538 6250 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1207 1171 274 183 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2275 2307 3468 3500 6252 6284 2275
Size: 32 1161 32 2752 32 2283
Fragments arranged by size:
2752 2283 1161 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2215 1316
Size: 899 5393
XbaI T'CTAG_A
Cuts at: 3628 3628
Size: 6292
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6292
XmaI C'CCGG_G
Cuts at: 1870 3487 3617 1870
Size: 1617 130 4545
Fragments arranged by size:
4545 1617 130
XmnI GAAnn'nnTTC
Cuts at: 4421 4421
Size: 6292
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4100 276
Size: 53 83 186 936 2566 2468
Fragments arranged by size:
2566 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspXI PsrI RsrII SacII SanDI SfiI SfoI
SrfI SwaI XcmI