Restriction map for
pFN21AE9457
(Circular) (Six-base) MAPSORT of: pFN21AE9457.seq Check: 2431 from: 1 to: 8668
pFN21AE9457 8668 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6478 278
Size: 53 83 186 936 4942 2468
Fragments arranged by size:
4942 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3650 6011 1937
Size: 1713 2361 4594
Fragments arranged by size:
4594 2361 1713
Acc65I G'GTAC_C
Cuts at: 5912 5989 5912
Size: 77 8591
AclI AA'CG_TT
Cuts at: 1563 6795 7168 1563
Size: 5232 373 3063
Fragments arranged by size:
5232 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2312 3070 3542 5101 6731 8115 8383 1632
Size: 680 758 472 1559 1630 1384 268 1917
Fragments arranged by size:
1917 1630 1559 1384 758 680 472 268
AfeI AGC'GCT
Cuts at: 2073 2073
Size: 8668
AflII C'TTAA_G
Cuts at: 820 1017 4859 820
Size: 197 3842 4629
Fragments arranged by size:
4629 3842 197
AflIII A'CryG_T
Cuts at: 1203 3499 7567 8544 1203
Size: 2296 4068 977 1327
Fragments arranged by size:
4068 2296 1327 977
AgeI A'CCGG_T
Cuts at: 7499 7499
Size: 8668
AhdI GACnn_n'nnGTC
Cuts at: 4074 5558 7397 4074
Size: 1484 1839 5345
Fragments arranged by size:
5345 1839 1484
AleI CACnn'nnGTG
Cuts at: 1166 2684 5142 1166
Size: 1518 2458 4692
Fragments arranged by size:
4692 2458 1518
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4744 5284 5382 5392 1184
Size: 3560 540 98 10 4460
Fragments arranged by size:
4460 3560 540 98 10
AlwNI CAG_nnn'CTG
Cuts at: 2224 2698 3337 3701 3953 4100 4476 6411 7983
Size: 474 639 364 252 147 376 1935 1572
Cuts at: 7983 2224
Size: 2909
Fragments arranged by size:
2909 1935 1572 639 474 376 364 252
147
ApaLI G'TGCA_C
Cuts at: 5501 6725 7881 5501
Size: 1224 1156 6288
Fragments arranged by size:
6288 1224 1156
ApoI r'AATT_y
Cuts at: 1493 2491 2552 3410 3958 3967 5326 5758 5977
Size: 998 61 858 548 9 1359 432 219
Cuts at: 5977 6203 6355 8464 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1359 998 858 548 432 226
219 152 61 9
AseI AT'TA_AT
Cuts at: 160 1032 7222 160
Size: 872 6190 1606
Fragments arranged by size:
6190 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8668
AvaI C'yCGr_G
Cuts at: 1870 1943 2151 5656 5993 1870
Size: 73 208 3505 337 4545
Fragments arranged by size:
4545 3505 337 208 73
BamHI G'GATC_C
Cuts at: 3736 4591 5998 6365 3736
Size: 855 1407 367 6039
Fragments arranged by size:
6039 1407 855 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2108 2174 2372 4161 5260
Size: 325 217 627 321 66 198 1789 1099
Cuts at: 5260 5470 5912 5989 7444 618
Size: 210 442 77 1455 1842
Fragments arranged by size:
1842 1789 1455 1099 627 442 325 321
217 210 198 77 66
BanII G_rGCy'C
Cuts at: 1391 3595 4869 5204 5987 1391
Size: 2204 1274 335 783 4072
Fragments arranged by size:
4072 2204 1274 783 335
BbeI G_GCGC'C
Cuts at: 2112 2178 5474 2112
Size: 66 3296 5306
Fragments arranged by size:
5306 3296 66
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3548 5283 928
Size: 971 1649 1735 4313
Fragments arranged by size:
4313 1735 1649 971
BbvCI CC'TCA_GC
Cuts at: 5412 5412
Size: 8668
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6859 6893 1903
Size: 34 4922 34 3678
Fragments arranged by size:
4922 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2629 4664 6560 7770 2629
Size: 2035 1896 1210 3527
Fragments arranged by size:
3527 2035 1896 1210
BclI T'GATC_A
Cuts at: 1548 2812 1548
Size: 1264 7404
BfrBI ATG'CAT
Cuts at: 2599 2599
Size: 8668
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2028 5637 7279
Size: 107 122 71 631 605 356 3609 1642
Cuts at: 7279 136
Size: 1525
Fragments arranged by size:
3609 1642 1525 631 605 356 122 107
71
BglII A'GATC_T
Cuts at: 8663 8663
Size: 8668
BlpI GC'TnA_GC
Cuts at: 3764 6085 3764
Size: 2321 6347
Bme1580I G_kGCm'C
Cuts at: 1790 2929 5505 6729 7885 1790
Size: 1139 2576 1224 1156 2573
Fragments arranged by size:
2576 2573 1224 1156 1139
BmgBI CAC'GTC
Cuts at: 1318 3457 1318
Size: 2139 6529
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6399 7357 442
Size: 416 53 83 399 24 4982 958 1753
Fragments arranged by size:
4982 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3584 1056
Size: 2528 6140
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 7328 1239
Size: 6089 2579
Bpu10I CC'TnA_GC
Cuts at: 4842 5412 4842
Size: 570 8098
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2496 4364 5491 6793 7658 7956 2496
Size: 1868 1127 1302 865 298 3208
Fragments arranged by size:
3208 1868 1302 1127 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7331 882
Size: 617 5832 2219
Fragments arranged by size:
5832 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5467 6459 493
Size: 711 4263 992 2702
Fragments arranged by size:
4263 2702 992 711
BsaBI GATnn'nnATC
Cuts at: 4370 8662 4370
Size: 4292 4376
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2020 2109 2175 5471
Size: 53 83 186 936 487 89 66 3296
Cuts at: 5471 6475 6857 7492 275
Size: 1004 382 635 1451
Fragments arranged by size:
3296 1451 1004 936 635 487 382 186
89 83 66 53
BsaWI w'CCGG_w
Cuts at: 7101 7499 7773 7920 8331 7101
Size: 398 274 147 411 7438
Fragments arranged by size:
7438 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2894 2924 3582 3612 1178
Size: 30 165 30 1491 30 658 30 6234
Fragments arranged by size:
6234 1491 658 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4304 4889 5674 1186
Size: 3118 585 785 4180
Fragments arranged by size:
4180 3118 785 585
BseYI C'CCAG_C
Cuts at: 2086 2604 5635 5709 7871 8367 2086
Size: 518 3031 74 2162 496 2387
Fragments arranged by size:
3031 2387 2162 518 496 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2218 3024 3569 5630 1163
Size: 573 482 806 545 2061 4201
Fragments arranged by size:
4201 2061 806 573 545 482
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6118 6879 7907 1540
Size: 275 186 4117 761 1028 2301
Fragments arranged by size:
4117 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3595 4974 5204 5505 6729 6814 7885 3595
Size: 1379 230 301 1224 85 1071 4378
Fragments arranged by size:
4378 1379 1224 1071 301 230 85
BsmI GAATG_Cn'
Cuts at: 2443 3923 4471 6179 6272 2443
Size: 1480 548 1708 93 4839
Fragments arranged by size:
4839 1708 1480 548 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2929 3595 4869 4974 5204 5505 5987
Size: 399 1139 666 1274 105 230 301 482
Cuts at: 5987 6729 6814 7885 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1274 1139 1071 742 666 482 399
301 230 105 85
BspEI T'CCGG_A
Cuts at: 8331 8331
Size: 8668
BspHI T'CATG_A
Cuts at: 5178 6557 8287 5178
Size: 1379 1730 5559
Fragments arranged by size:
5559 1730 1379
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2209 6023 844
Size: 1365 3814 3489
Fragments arranged by size:
3814 3489 1365
BsrBI CCG'CTC
Cuts at: 1394 1823 2059 6555 1394
Size: 429 236 4496 3507
Fragments arranged by size:
4496 3507 429 236
BsrDI GCAATG_nn'
Cuts at: 65 1248 3468 3564 3784 3918 3937 5893 5935
Size: 1183 2220 96 220 134 19 1956 42
Cuts at: 5935 7163 7337 65
Size: 1228 174 1396
Fragments arranged by size:
2220 1956 1396 1228 1183 220 174 134
96 42 19
BsrFI r'CCGG_y
Cuts at: 1700 1810 2022 2126 3576 3584 5631 7312 7499
Size: 110 212 104 1450 8 2047 1681 187
Cuts at: 7499 1700
Size: 2869
Fragments arranged by size:
2869 2047 1681 1450 212 187 110 104
8
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8668
BssHII G'CGCG_C
Cuts at: 1925 2034 1925
Size: 109 8559
BssSI C'ACGA_G
Cuts at: 6728 7740 6728
Size: 1012 7656
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2224 794
Size: 1430 7238
BstBI TT'CG_AA
Cuts at: 5491 7472 5491
Size: 1981 6687
BstEII G'GTnAC_C
Cuts at: 1184 2254 4000 1184
Size: 1070 1746 5852
Fragments arranged by size:
5852 1746 1070
BstXI CCAn_nnnn'nTGG
Cuts at: 3103 3152 3316 4487 3103
Size: 49 164 1171 7284
Fragments arranged by size:
7284 1171 164 49
BstYI r'GATC_y
Cuts at: 1972 2365 2407 2954 3736 4247 4591 5998 6365
Size: 393 42 547 782 511 344 1407 367
Cuts at: 6365 6750 6767 8219 8305 8317 8663 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1407 782 547 511 393 385
367 346 344 86 42 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2112 3145 3315 4869 513
Size: 552 939 108 1033 170 1554 4312
Fragments arranged by size:
4312 1554 1033 939 552 170 108
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2585 2738 5739 487
Size: 683 56 1359 153 3001 3416
Fragments arranged by size:
3416 3001 1359 683 153 56
BtsI GCAGTG_nn'
Cuts at: 2660 3134 3157 3557 4070 5287 6184 6978 6998
Size: 474 23 400 513 1217 897 794 20
Cuts at: 6998 2660
Size: 4330
Fragments arranged by size:
4330 1217 897 794 513 474 400 23
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8668
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2813 2848 5577 5612 529
Size: 35 2249 35 2729 35 3585
Fragments arranged by size:
3585 2729 2249 35 35 35
DraI TTT'AAA
Cuts at: 5972 5972
Size: 8668
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7675 809
Size: 507 6359 1802
Fragments arranged by size:
6359 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4269 4712 5859
Size: 54 1425 50 275 18 2439 443 1147
Cuts at: 5859 6115 7004 8633 8
Size: 256 889 1629 43
Fragments arranged by size:
2439 1629 1425 1147 889 443 275 256
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6115 1537
Size: 275 4303 4090
Fragments arranged by size:
4303 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5662 6598 1353
Size: 4309 936 3423
Fragments arranged by size:
4309 3423 936
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3042 7255 7629 7775 387
Size: 1064 138 1453 4213 374 146 1280
Fragments arranged by size:
4213 1453 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3593 5202 3593
Size: 1609 7059
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2312 3070 3542 5101 6731 7328 8115
Size: 393 680 758 472 1559 1630 597 787
Cuts at: 8115 8383 1239
Size: 268 1524
Fragments arranged by size:
1630 1559 1524 787 758 680 597 472
393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3538 3538
Size: 8668
EcoO109I rG'GnC_Cy
Cuts at: 2221 5900 2221
Size: 3679 4989
EcoRI G'AATT_C
Cuts at: 2552 5977 6355 2552
Size: 3425 378 4865
Fragments arranged by size:
4865 3425 378
EcoRV GAT'ATC
Cuts at: 2283 2283
Size: 8668
FspI TGC'GCA
Cuts at: 7174 7174
Size: 8668
HaeII r_GCGC'y
Cuts at: 1678 2075 2112 2120 2178 5474 7815 1678
Size: 397 37 8 58 3296 2341 2531
Fragments arranged by size:
3296 2531 2341 397 58 37 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2322 2354 2404 2436 2551
Size: 32 135 32 750 32 50 32 115
Cuts at: 2551 2583 2734 2766 3422 3454 3721 3753 4060
Size: 32 151 32 656 32 267 32 307
Cuts at: 4060 4092 4611 4643 4916 4948 5544 5576 5892
Size: 32 519 32 273 32 596 32 316
Cuts at: 5892 5924 7309 7341 7383 7415 1373
Size: 32 1385 32 42 32 2626
Fragments arranged by size:
2626 1385 750 656 596 519 316 307
273 267 151 135 115 50 42 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2703 3466 5221 6012 6258 669
Size: 988 281 765 763 1755 791 246 3079
Fragments arranged by size:
3079 1755 988 791 765 763 281 246
HindIII A'AGCT_T
Cuts at: 748 3189 3633 3908 3938 5113 5317 748
Size: 2441 444 275 30 1175 204 4099
Fragments arranged by size:
4099 2441 1175 444 275 204 30
HpaI GTT'AAC
Cuts at: 6258 6258
Size: 8668
KasI G'GCGC_C
Cuts at: 2108 2174 5470 2108
Size: 66 3296 5306
Fragments arranged by size:
5306 3296 66
KpnI G_GTAC'C
Cuts at: 5916 5993 5916
Size: 77 8591
MfeI C'AATT_G
Cuts at: 6267 6267
Size: 8668
MluI A'CGCG_T
Cuts at: 8544 8544
Size: 8668
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7782 7966 1305
Size: 6477 184 2007
Fragments arranged by size:
6477 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5861 10
Size: 54 1425 343 4029 2817
Fragments arranged by size:
4029 2817 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2684 2825 3150 3665 4397 5142 5266
Size: 648 1518 141 325 515 732 745 124
Cuts at: 5266 6626 6985 7144 518
Size: 1360 359 159 2042
Fragments arranged by size:
2042 1518 1360 745 732 648 515 359
325 159 141 124
MspA1I CmG'CkG
Cuts at: 1606 2047 2114 3763 5813 6084 6761 7909 8154
Size: 441 67 1649 2050 271 677 1148 245
Cuts at: 8154 1606
Size: 2120
Fragments arranged by size:
2120 2050 1649 1148 677 441 271 245
67
NaeI GCC'GGC
Cuts at: 2024 2128 5633 2024
Size: 104 3505 5059
Fragments arranged by size:
5059 3505 104
NarI GG'CG_CC
Cuts at: 2109 2175 5471 2109
Size: 66 3296 5306
Fragments arranged by size:
5306 3296 66
NcoI C'CATG_G
Cuts at: 513 1065 2004 3145 513
Size: 552 939 1141 6036
Fragments arranged by size:
6036 1141 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8668
NgoMIV G'CCGG_C
Cuts at: 2022 2126 5631 2022
Size: 104 3505 5059
Fragments arranged by size:
5059 3505 104
NheI G'CTAG_C
Cuts at: 1052 3580 1052
Size: 2528 6140
NotI GC'GGCC_GC
Cuts at: 6115 6115
Size: 8668
NsiI A_TGCA'T
Cuts at: 2601 2601
Size: 8668
NspI r_CATG'y
Cuts at: 1132 1229 2709 2824 4396 4804 6026 7571 1132
Size: 97 1480 115 1572 408 1222 1545 2229
Fragments arranged by size:
2229 1572 1545 1480 1222 408 115 97
PasI CC'CwG_GG
Cuts at: 1348 5335 1348
Size: 3987 4681
PciI A'CATG_T
Cuts at: 7567 7567
Size: 8668
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2797 3691 3953 1108
Size: 1689 894 262 5823
Fragments arranged by size:
5823 1689 894 262
PfoI T'CCnGG_A
Cuts at: 3163 3163
Size: 8668
PmeI GTTT'AAAC
Cuts at: 5972 5972
Size: 8668
PmlI CAC'GTG
Cuts at: 5467 5467
Size: 8668
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6737 6769 8250 8282 6737
Size: 32 1481 32 7123
Fragments arranged by size:
7123 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2221 5900 2221
Size: 3679 4989
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8668
PsiI TTA'TAA
Cuts at: 6238 6238
Size: 8668
PspXI vC'TCGA_Gb
Cuts at: 5656 5656
Size: 8668
PstI C_TGCA'G
Cuts at: 830 2667 3129 3539 4684 5371 6020 830
Size: 1837 462 410 1145 687 649 3478
Fragments arranged by size:
3478 1837 1145 687 649 462 410
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8668
PvuII CAG'CTG
Cuts at: 3763 5813 3763
Size: 2050 6618
SacI G_AGCT'C
Cuts at: 3595 5204 3595
Size: 1609 7059
SacII CC_GC'GG
Cuts at: 2115 2115
Size: 8668
SalI G'TCGA_C
Cuts at: 1936 6010 1936
Size: 4074 4594
SanDI GG'GwC_CC
Cuts at: 5900 5900
Size: 8668
SapI GCTCTTCn'nnn_
Cuts at: 5662 5662
Size: 8668
SbfI CC_TGCA'GG
Cuts at: 6020 6020
Size: 8668
ScaI AGT'ACT
Cuts at: 3047 4524 6916 8628 3047
Size: 1477 2392 1712 3087
Fragments arranged by size:
3087 2392 1712 1477
SexAI A'CCwGG_T
Cuts at: 2639 2639
Size: 8668
SfcI C'TryA_G
Cuts at: 826 1045 2663 3125 3535 4680 5367 5683 6016
Size: 219 1618 462 410 1145 687 316 333
Cuts at: 6016 7151 7832 8023 826
Size: 1135 681 191 1471
Fragments arranged by size:
1618 1471 1145 1135 687 681 462 410
333 316 219 191
SfoI GGC'GCC
Cuts at: 2110 2176 5472 2110
Size: 66 3296 5306
Fragments arranged by size:
5306 3296 66
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8668
SgrAI Cr'CCGG_yG
Cuts at: 1700 2022 1700
Size: 322 8346
SmaI CCC'GGG
Cuts at: 1872 5995 1872
Size: 4123 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2511 4379 4859 5506 5656 6772
Size: 197 926 568 1868 480 647 150 1116
Cuts at: 6772 7673 7935 820
Size: 901 262 1553
Fragments arranged by size:
1868 1553 1116 926 901 647 568 480
262 197 150
SnaBI TAC'GTA
Cuts at: 493 6459 493
Size: 5966 2702
SpeI A'CTAG_T
Cuts at: 152 5783 152
Size: 5631 3037
SphI G_CATG'C
Cuts at: 1132 6026 1132
Size: 4894 3774
SspI AAT'ATT
Cuts at: 5 52 2944 3622 6592 5
Size: 47 2892 678 2970 2081
Fragments arranged by size:
2970 2892 2081 678 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8668
StyI C'CwwG_G
Cuts at: 513 1065 2004 3145 3426 4752 4959 6107 513
Size: 552 939 1141 281 1326 207 1148 3074
Fragments arranged by size:
3074 1326 1148 1141 939 552 281 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3158 3631 4149 4277 5278 6695 6712
Size: 1255 1578 473 518 128 1001 1417 17
Cuts at: 6712 6865 7050 7495 325
Size: 153 185 445 1498
Fragments arranged by size:
1578 1498 1417 1255 1001 518 473 445
185 153 128 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3045 4522 4803
Size: 274 80 33 51 1443 1068 1477 281
Cuts at: 4803 5697 6914 8626 96
Size: 894 1217 1712 138
Fragments arranged by size:
1712 1477 1443 1217 1068 894 281 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2627 2659 2894 2926 8628 8660 2627
Size: 32 235 32 5702 32 2635
Fragments arranged by size:
5702 2635 235 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8668
XbaI T'CTAG_A
Cuts at: 6004 6004
Size: 8668
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2376 2990 3625 2376
Size: 614 635 7419
Fragments arranged by size:
7419 635 614
XhoI C'TCGA_G
Cuts at: 1943 5656 1943
Size: 3713 4955
XmaI C'CCGG_G
Cuts at: 1870 5993 1870
Size: 4123 4545
XmnI GAAnn'nnTTC
Cuts at: 6797 6797
Size: 8668
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6476 276
Size: 53 83 186 936 4942 2468
Fragments arranged by size:
4942 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII FalI FseI FspAI NruI
PacI PspOMI PsrI RsrII SfiI SrfI SwaI