Restriction map for
pFN21AE9456
(Circular) (Six-base) MAPSORT of: pFN21AE9456.seq Check: 1427 from: 1 to: 6790
pFN21AE9456 6790 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3227 3227
Size: 6790
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4600 278
Size: 53 83 186 936 3064 2468
Fragments arranged by size:
3064 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4133 1937
Size: 2196 4594
Acc65I G'GTAC_C
Cuts at: 4111 4111
Size: 6790
AclI AA'CG_TT
Cuts at: 1563 4917 5290 1563
Size: 3354 373 3063
Fragments arranged by size:
3354 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2142 4034 4853 6237 6505 1632
Size: 510 1892 819 1384 268 1917
Fragments arranged by size:
1917 1892 1384 819 510 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6593
AflIII A'CryG_T
Cuts at: 1203 2234 2270 3324 3417 5689 6666 1203
Size: 1031 36 1054 93 2272 977 1327
Fragments arranged by size:
2272 1327 1054 1031 977 93 36
AgeI A'CCGG_T
Cuts at: 5621 5621
Size: 6790
AhdI GACnn_n'nnGTC
Cuts at: 5519 5519
Size: 6790
AleI CACnn'nnGTG
Cuts at: 1166 3149 1166
Size: 1983 4807
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6790
AlwNI CAG_nnn'CTG
Cuts at: 4533 6105 4533
Size: 1572 5218
ApaLI G'TGCA_C
Cuts at: 4847 6003 4847
Size: 1156 5634
ApoI r'AATT_y
Cuts at: 1493 2659 4099 4325 4477 6586 1493
Size: 1166 1440 226 152 2109 1697
Fragments arranged by size:
2109 1697 1440 1166 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4073 5344 160
Size: 872 3041 1271 1606
Fragments arranged by size:
3041 1606 1271 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6790
AvaI C'yCGr_G
Cuts at: 1870 1943 2541 3288 4115 1870
Size: 73 598 747 827 4545
Fragments arranged by size:
4545 827 747 598 73
BamHI G'GATC_C
Cuts at: 2145 4120 4487 2145
Size: 1975 367 4448
Fragments arranged by size:
4448 1975 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3576 4111 5566 618
Size: 325 217 627 1789 535 1455 1842
Fragments arranged by size:
1842 1789 1455 627 535 325 217
BanII G_rGCy'C
Cuts at: 1391 2377 3046 3231 4109 1391
Size: 986 669 185 878 4072
Fragments arranged by size:
4072 986 878 669 185
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2930 3999 928
Size: 971 1031 1069 3719
Fragments arranged by size:
3719 1069 1031 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4981 5015 1903
Size: 34 3044 34 3678
Fragments arranged by size:
3678 3044 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4682 5892 4682
Size: 1210 5580
BclI T'GATC_A
Cuts at: 1548 3264 3585 1548
Size: 1716 321 4753
Fragments arranged by size:
4753 1716 321
BfrBI ATG'CAT
Cuts at: 2864 4043 2864
Size: 1179 5611
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3698 5401 136
Size: 107 122 71 631 605 2026 1703 1525
Fragments arranged by size:
2026 1703 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6785 6785
Size: 6790
BlpI GC'TnA_GC
Cuts at: 3230 4207 3230
Size: 977 5813
Bme1580I G_kGCm'C
Cuts at: 1790 3581 4851 6007 1790
Size: 1791 1270 1156 2573
Fragments arranged by size:
2573 1791 1270 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6790
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2617 3040 3670
Size: 416 53 83 399 24 1200 423 630
Cuts at: 3670 4521 5479 442
Size: 851 958 1753
Fragments arranged by size:
1753 1200 958 851 630 423 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6790
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2415 2415
Size: 6790
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2121 2361 5450 1239
Size: 882 240 3089 2579
Fragments arranged by size:
3089 2579 882 240
Bpu10I CC'TnA_GC
Cuts at: 2205 2843 3245 2205
Size: 638 402 5750
Fragments arranged by size:
5750 638 402
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2385 4915 5780 6078 2385
Size: 2530 865 298 3097
Fragments arranged by size:
3097 2530 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2375 3631 5453 882
Size: 617 876 1256 1822 2219
Fragments arranged by size:
2219 1822 1256 876 617
BsaAI yAC'GTr
Cuts at: 493 1204 3325 4581 493
Size: 711 2121 1256 2702
Fragments arranged by size:
2702 2121 1256 711
BsaBI GATnn'nnATC
Cuts at: 6784 6784
Size: 6790
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4597 4979 5614 275
Size: 53 83 186 936 3064 382 635 1451
Fragments arranged by size:
3064 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5223 5621 5895 6042 6453 5223
Size: 398 274 147 411 5560
Fragments arranged by size:
5560 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6565
Fragments arranged by size:
6565 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6790
BseYI C'CCAG_C
Cuts at: 3844 5993 6489 3844
Size: 2149 496 4145
Fragments arranged by size:
4145 2149 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6217
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4240 5001 6029 1540
Size: 275 186 2239 761 1028 2301
Fragments arranged by size:
2301 2239 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2377 4851 4936 6007 2377
Size: 2474 85 1071 3160
Fragments arranged by size:
3160 2474 1071 85
BsmI GAATG_Cn'
Cuts at: 4045 4301 4394 4045
Size: 256 93 6441
Fragments arranged by size:
6441 256 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3241 3241
Size: 6790
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2377 3046 3231 3581 4109 4851 4936
Size: 399 587 669 185 350 528 742 85
Cuts at: 4936 6007 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 669 587 528 399 350
185 85
BspEI T'CCGG_A
Cuts at: 6453 6453
Size: 6790
BspHI T'CATG_A
Cuts at: 4679 6409 4679
Size: 1730 5060
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3227 4145 844
Size: 2383 918 3489
Fragments arranged by size:
3489 2383 918
BsrBI CCG'CTC
Cuts at: 1394 1823 4677 1394
Size: 429 2854 3507
Fragments arranged by size:
3507 2854 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3040 3488 3588 5285 5459 65
Size: 1183 1792 448 100 1697 174 1396
Fragments arranged by size:
1792 1697 1396 1183 448 174 100
BsrFI r'CCGG_y
Cuts at: 1700 1810 5434 5621 1700
Size: 110 3624 187 2869
Fragments arranged by size:
3624 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2273 96
Size: 2177 4613
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6790
BssSI C'ACGA_G
Cuts at: 4850 5862 4850
Size: 1012 5778
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6790
BstBI TT'CG_AA
Cuts at: 3012 5594 3012
Size: 2582 4208
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6790
BstXI CCAn_nnnn'nTGG
Cuts at: 3053 3053
Size: 6790
BstYI r'GATC_y
Cuts at: 1972 2078 2145 4064 4120 4487 4872 4889 6341
Size: 106 67 1919 56 367 385 17 1452
Cuts at: 6341 6427 6439 6785 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1919 1452 385 367 346 106 86
67 56 17 12
Bsu36I CC'TnA_GG
Cuts at: 3642 3642
Size: 6790
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6238
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6051
Fragments arranged by size:
6051 683 56
BtsI GCAGTG_nn'
Cuts at: 3351 3435 3839 4306 5100 5120 3351
Size: 84 404 467 794 20 5021
Fragments arranged by size:
5021 794 467 404 84 20
ClaI AT'CG_AT
Cuts at: 1554 3188 1554
Size: 1634 5156
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2231 2266 529
Size: 35 1667 35 5053
Fragments arranged by size:
5053 1667 35 35
DraI TTT'AAA
Cuts at: 4094 4094
Size: 6790
DraIII CAC_nnn'GTG
Cuts at: 3282 3282
Size: 6790
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5797 809
Size: 507 4481 1802
Fragments arranged by size:
4481 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2571 2855 4237
Size: 54 1425 50 275 18 741 284 1382
Cuts at: 4237 5126 6755 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1382 889 741 284 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4237 1537
Size: 275 2425 4090
Fragments arranged by size:
4090 2425 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2549 2723 3485 4720 1353
Size: 1196 174 762 1235 3423
Fragments arranged by size:
3423 1235 1196 762 174
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5377 5751 5897 387
Size: 1064 138 3788 374 146 1280
Fragments arranged by size:
3788 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2375 2375
Size: 6790
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2121 2142 2361 4034 4853 5450 6237
Size: 393 489 21 219 1673 819 597 787
Cuts at: 6237 6505 1239
Size: 268 1524
Fragments arranged by size:
1673 1524 819 787 597 489 393 268
219 21
EcoO109I rG'GnC_Cy
Cuts at: 2165 3762 2165
Size: 1597 5193
EcoRI G'AATT_C
Cuts at: 4099 4477 4099
Size: 378 6412
EcoRV GAT'ATC
Cuts at: 2809 3112 3309 2809
Size: 303 197 6290
Fragments arranged by size:
6290 303 197
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4005 4005
Size: 6790
FspI TGC'GCA
Cuts at: 5296 5296
Size: 6790
HaeII r_GCGC'y
Cuts at: 1678 5937 1678
Size: 4259 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4028 4060 5431 5463 5505
Size: 32 135 32 2456 32 1371 32 42
Cuts at: 5505 5537 1373
Size: 32 2626
Fragments arranged by size:
2626 2456 1371 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3751 4134 4380 669
Size: 988 281 1813 383 246 3079
Fragments arranged by size:
3079 1813 988 383 281 246
HindIII A'AGCT_T
Cuts at: 748 3247 748
Size: 2499 4291
HpaI GTT'AAC
Cuts at: 4380 4380
Size: 6790
KpnI G_GTAC'C
Cuts at: 4115 4115
Size: 6790
MfeI C'AATT_G
Cuts at: 4389 4389
Size: 6790
MluI A'CGCG_T
Cuts at: 6666 6666
Size: 6790
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2208 3087 5904 6088 1305
Size: 903 879 2817 184 2007
Fragments arranged by size:
2817 2007 903 879 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2573 2857 10
Size: 54 1425 343 741 284 3943
Fragments arranged by size:
3943 1425 741 343 284 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2447 2786 3149 3173 3803 4748
Size: 648 843 438 339 363 24 630 945
Cuts at: 4748 5107 5266 518
Size: 359 159 2042
Fragments arranged by size:
2042 945 843 648 630 438 363 359
339 159 24
MspA1I CmG'CkG
Cuts at: 1606 2264 3135 4034 4206 4883 6031 6276 1606
Size: 658 871 899 172 677 1148 245 2120
Fragments arranged by size:
2120 1148 899 871 677 658 245 172
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6238
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6790
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6790
NotI GC'GGCC_GC
Cuts at: 4237 4237
Size: 6790
NsiI A_TGCA'T
Cuts at: 2866 4045 2866
Size: 1179 5611
NspI r_CATG'y
Cuts at: 1132 1229 2238 2274 3319 3421 4148 5693 1132
Size: 97 1009 36 1045 102 727 1545 2229
Fragments arranged by size:
2229 1545 1045 1009 727 102 97 36
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6790
PciI A'CATG_T
Cuts at: 2234 2270 3417 5689 2234
Size: 36 1147 2272 3335
Fragments arranged by size:
3335 2272 1147 36
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3282 3369 4061 1108
Size: 2174 87 692 3837
Fragments arranged by size:
3837 2174 692 87
PmeI GTTT'AAAC
Cuts at: 4094 4094
Size: 6790
PmlI CAC'GTG
Cuts at: 3325 3325
Size: 6790
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4859 4891 6372 6404 4859
Size: 32 1481 32 5245
Fragments arranged by size:
5245 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2165 2165
Size: 6790
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6790
PsiI TTA'TAA
Cuts at: 4360 4360
Size: 6790
PstI C_TGCA'G
Cuts at: 830 2192 4142 830
Size: 1362 1950 3478
Fragments arranged by size:
3478 1950 1362
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6790
PvuII CAG'CTG
Cuts at: 2264 4034 2264
Size: 1770 5020
SacI G_AGCT'C
Cuts at: 2377 2377
Size: 6790
SalI G'TCGA_C
Cuts at: 1936 4132 1936
Size: 2196 4594
SapI GCTCTTCn'nnn_
Cuts at: 2549 3485 2549
Size: 936 5854
SbfI CC_TGCA'GG
Cuts at: 4142 4142
Size: 6790
ScaI AGT'ACT
Cuts at: 5038 6750 5038
Size: 1712 5078
SfcI C'TryA_G
Cuts at: 826 1045 2074 2188 2331 2517 2560 3600 4138
Size: 219 1029 114 143 186 43 1040 538
Cuts at: 4138 5273 5954 6145 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1040 1029 681 538 219 191
186 143 114 43
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6790
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6790
SmaI CCC'GGG
Cuts at: 1872 3290 4117 1872
Size: 1418 827 4545
Fragments arranged by size:
4545 1418 827
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2400 2541 4894 5795 6057 820
Size: 197 926 457 141 2353 901 262 1553
Fragments arranged by size:
2353 1553 926 901 457 262 197 141
SnaBI TAC'GTA
Cuts at: 493 4581 493
Size: 4088 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6790
SphI G_CATG'C
Cuts at: 1132 4148 1132
Size: 3016 3774
SspI AAT'ATT
Cuts at: 5 52 2482 2512 3510 4714 5
Size: 47 2430 30 998 1204 2081
Fragments arranged by size:
2430 2081 1204 998 47 30
StuI AGG'CCT
Cuts at: 1517 3647 1517
Size: 2130 4660
StyI C'CwwG_G
Cuts at: 513 1065 2895 4229 513
Size: 552 1830 1334 3074
Fragments arranged by size:
3074 1830 1334 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3209 3564 4817 4834 4987 5172 5617
Size: 1255 1629 355 1253 17 153 185 445
Cuts at: 5617 325
Size: 1498
Fragments arranged by size:
1629 1498 1255 1253 445 355 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2273 5036 6748
Size: 274 80 33 51 1443 296 2763 1712
Cuts at: 6748 96
Size: 138
Fragments arranged by size:
2763 1712 1443 296 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2063 2095 6750 6782 2063
Size: 32 4655 32 2071
Fragments arranged by size:
4655 2071 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6790
XbaI T'CTAG_A
Cuts at: 2362 4126 2362
Size: 1764 5026
XhoI C'TCGA_G
Cuts at: 1943 2541 1943
Size: 598 6192
XmaI C'CCGG_G
Cuts at: 1870 3288 4115 1870
Size: 1418 827 4545
Fragments arranged by size:
4545 1418 827
XmnI GAAnn'nnTTC
Cuts at: 4919 4919
Size: 6790
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4598 276
Size: 53 83 186 936 3064 2468
Fragments arranged by size:
3064 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BsiWI BstZ17I EcoNI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PspOMI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI