Restriction map for
pFN21AE9452
(Circular) (Six-base) MAPSORT of: pFN21AE9452.seq Check: 4226 from: 1 to: 6661
pFN21AE9452 6661 bp
With 182 enzymes: SgfI *
January 30, 2014 17:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2144 2144
Size: 6661
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4471 278
Size: 53 83 186 936 2935 2468
Fragments arranged by size:
2935 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2919 3795 4004 1937
Size: 982 876 209 4594
Fragments arranged by size:
4594 982 876 209
Acc65I G'GTAC_C
Cuts at: 3982 3982
Size: 6661
AclI AA'CG_TT
Cuts at: 1563 4788 5161 1563
Size: 3225 373 3063
Fragments arranged by size:
3225 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2637 4724 6108 6376 1632
Size: 1005 2087 1384 268 1917
Fragments arranged by size:
2087 1917 1384 1005 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6464
AflIII A'CryG_T
Cuts at: 1203 2195 2841 2977 5560 6537 1203
Size: 992 646 136 2583 977 1327
Fragments arranged by size:
2583 1327 992 977 646 136
AgeI A'CCGG_T
Cuts at: 5492 5492
Size: 6661
AhdI GACnn_n'nnGTC
Cuts at: 5390 5390
Size: 6661
AleI CACnn'nnGTG
Cuts at: 1166 3086 1166
Size: 1920 4741
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3191 1184
Size: 2007 4654
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2538 2570 2538
Size: 32 6629
AlwNI CAG_nnn'CTG
Cuts at: 4404 5976 4404
Size: 1572 5089
ApaLI G'TGCA_C
Cuts at: 3329 4718 5874 3329
Size: 1389 1156 4116
Fragments arranged by size:
4116 1389 1156
ApoI r'AATT_y
Cuts at: 1493 2417 3559 3970 4196 4348 6457 1493
Size: 924 1142 411 226 152 2109 1697
Fragments arranged by size:
2109 1697 1142 924 411 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2208 5215 160
Size: 872 1176 3007 1606
Fragments arranged by size:
3007 1606 1176 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6661
AvaI C'yCGr_G
Cuts at: 1870 1943 3986 1870
Size: 73 2043 4545
Fragments arranged by size:
4545 2043 73
BamHI G'GATC_C
Cuts at: 3061 3991 4358 3061
Size: 930 367 5364
Fragments arranged by size:
5364 930 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3982 5437 618
Size: 325 217 627 2195 1455 1842
Fragments arranged by size:
2195 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3980 1391
Size: 2589 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2902 928
Size: 971 1003 4687
Fragments arranged by size:
4687 1003 971
BbvCI CC'TCA_GC
Cuts at: 3806 3806
Size: 6661
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4852 4886 1903
Size: 34 50 34 2831 34 3678
Fragments arranged by size:
3678 2831 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4553 5763 4553
Size: 1210 5451
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6661
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3774 5272 136
Size: 107 122 71 631 605 2102 1498 1525
Fragments arranged by size:
2102 1525 1498 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6656 6656
Size: 6661
BlpI GC'TnA_GC
Cuts at: 2045 3095 3672 4078 2045
Size: 1050 577 406 4628
Fragments arranged by size:
4628 1050 577 406
Bme1580I G_kGCm'C
Cuts at: 1790 2099 3333 4722 5878 1790
Size: 309 1234 1389 1156 2573
Fragments arranged by size:
2573 1389 1234 1156 309
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6661
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2700 2889 3404
Size: 416 53 83 399 24 1283 189 515
Cuts at: 3404 3530 3912 4392 5350 442
Size: 126 382 480 958 1753
Fragments arranged by size:
1753 1283 958 515 480 416 399 382
189 126 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6661
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3120 3120
Size: 6661
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3341 5321 1239
Size: 2102 1980 2579
Fragments arranged by size:
2579 2102 1980
Bpu10I CC'TnA_GC
Cuts at: 3806 3806
Size: 6661
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2577 3358 4786 5651 5949 2577
Size: 781 1428 865 298 3289
Fragments arranged by size:
3289 1428 865 781 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5324 882
Size: 617 3825 2219
Fragments arranged by size:
3825 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2881 2980 3088 4452 493
Size: 711 1677 99 108 1364 2702
Fragments arranged by size:
2702 1677 1364 711 108 99
BsaBI GATnn'nnATC
Cuts at: 6655 6655
Size: 6661
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4468 4850 5485 275
Size: 53 83 186 936 2935 382 635 1451
Fragments arranged by size:
2935 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5094 5492 5766 5913 6324 5094
Size: 398 274 147 411 5431
Fragments arranged by size:
5431 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2351 2381 1178
Size: 30 165 30 948 30 5458
Fragments arranged by size:
5458 948 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2359 3071 3107 3206 3794 1186
Size: 1173 712 36 99 588 4053
Fragments arranged by size:
4053 1173 712 588 99 36
BseYI C'CCAG_C
Cuts at: 5864 6360 5864
Size: 496 6165
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2116 3330 3676 1163
Size: 573 380 1214 346 4148
Fragments arranged by size:
4148 1214 573 380 346
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4111 4872 5900 1540
Size: 275 186 2110 761 1028 2301
Fragments arranged by size:
2301 2110 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2933 3263 3333 4722 4807 5878 2933
Size: 330 70 1389 85 1071 3716
Fragments arranged by size:
3716 1389 1071 330 85 70
BsmI GAATG_Cn'
Cuts at: 2311 4172 4265 2311
Size: 1861 93 4707
Fragments arranged by size:
4707 1861 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3106 3106
Size: 6661
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2099 2933 3263 3333 3980 4722 4807
Size: 399 309 834 330 70 647 742 85
Cuts at: 4807 5878 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 834 742 647 399 330 309
85 70
BspEI T'CCGG_A
Cuts at: 6324 6324
Size: 6661
BspHI T'CATG_A
Cuts at: 3390 4550 6280 3390
Size: 1160 1730 3771
Fragments arranged by size:
3771 1730 1160
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2144 3805 4016 844
Size: 1300 1661 211 3489
Fragments arranged by size:
3489 1661 1300 211
BsrBI CCG'CTC
Cuts at: 1394 1823 4548 1394
Size: 429 2725 3507
Fragments arranged by size:
3507 2725 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2015 2712 5156 5330 65
Size: 1183 767 697 2444 174 1396
Fragments arranged by size:
2444 1396 1183 767 697 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3272 5305 5492 1700
Size: 110 1462 2033 187 2869
Fragments arranged by size:
2869 2033 1462 187 110
BsrGI T'GTAC_A
Cuts at: 96 2216 96
Size: 2120 4541
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6661
BssSI C'ACGA_G
Cuts at: 3224 4721 5733 3224
Size: 1497 1012 4152
Fragments arranged by size:
4152 1497 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6661
BstBI TT'CG_AA
Cuts at: 5465 5465
Size: 6661
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6661
BstXI CCAn_nnnn'nTGG
Cuts at: 3898 3898
Size: 6661
BstYI r'GATC_y
Cuts at: 1972 2159 2539 3061 3991 4358 4743 4760 6212
Size: 187 380 522 930 367 385 17 1452
Cuts at: 6212 6298 6310 6656 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 930 522 385 380 367 346
187 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2143 513
Size: 552 1078 5031
Fragments arranged by size:
5031 1078 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3253 487
Size: 683 56 2027 3895
Fragments arranged by size:
3895 2027 683 56
BtsI GCAGTG_nn'
Cuts at: 2128 2680 3173 3342 4177 4971 4991 2128
Size: 552 493 169 835 794 20 3798
Fragments arranged by size:
3798 835 794 552 493 169 20
ClaI AT'CG_AT
Cuts at: 1554 3487 1554
Size: 1933 4728
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6626
DraI TTT'AAA
Cuts at: 3965 3965
Size: 6661
DraIII CAC_nnn'GTG
Cuts at: 3000 3699 3000
Size: 699 5962
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5668 809
Size: 507 4352 1802
Fragments arranged by size:
4352 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3297 3441 4108
Size: 54 1425 50 275 18 1467 144 667
Cuts at: 4108 4997 6626 8
Size: 889 1629 43
Fragments arranged by size:
1629 1467 1425 889 667 275 144 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4108 1537
Size: 275 2296 4090
Fragments arranged by size:
4090 2296 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2763 2921 3206 3224 3341 3683 3755 3944
Size: 1410 158 285 18 117 342 72 189
Cuts at: 3944 4591 1353
Size: 647 3423
Fragments arranged by size:
3423 1410 647 342 285 189 158 117
72 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5248 5622 5768 387
Size: 1064 138 3659 374 146 1280
Fragments arranged by size:
3659 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2637 3341 4724 5321 6108 6376 1239
Size: 393 1005 704 1383 597 787 268 1524
Fragments arranged by size:
1524 1383 1005 787 704 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3506 3506
Size: 6661
EcoO109I rG'GnC_Cy
Cuts at: 2702 3902 2702
Size: 1200 5461
EcoRI G'AATT_C
Cuts at: 3970 4348 3970
Size: 378 6283
FspI TGC'GCA
Cuts at: 5167 5167
Size: 6661
HaeII r_GCGC'y
Cuts at: 1678 5808 1678
Size: 4130 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2351 2383 2457 2489 2495
Size: 32 135 32 779 32 74 32 6
Cuts at: 2495 2527 2597 2629 3480 3512 5302 5334 5376
Size: 32 70 32 851 32 1790 32 42
Cuts at: 5376 5408 1373
Size: 32 2626
Fragments arranged by size:
2626 1790 851 779 135 74 70 42
32 32 32 32 32 32 32 32
32 6
HincII GTy'rAC
Cuts at: 669 1657 1938 3367 3630 4005 4251 669
Size: 988 281 1429 263 375 246 3079
Fragments arranged by size:
3079 1429 988 375 281 263 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6661
HpaI GTT'AAC
Cuts at: 4251 4251
Size: 6661
KpnI G_GTAC'C
Cuts at: 3986 3986
Size: 6661
MfeI C'AATT_G
Cuts at: 2608 4260 2608
Size: 1652 5009
MluI A'CGCG_T
Cuts at: 6537 6537
Size: 6661
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5775 5959 1305
Size: 4470 184 2007
Fragments arranged by size:
4470 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3443 10
Size: 54 1425 343 1611 3228
Fragments arranged by size:
3228 1611 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3086 3311 3471 3647 4619 4978 5137
Size: 648 1920 225 160 176 972 359 159
Cuts at: 5137 518
Size: 2042
Fragments arranged by size:
2042 1920 972 648 359 225 176 160
159
MspA1I CmG'CkG
Cuts at: 1606 3431 3728 4077 4754 5902 6147 1606
Size: 1825 297 349 677 1148 245 2120
Fragments arranged by size:
2120 1825 1148 677 349 297 245
NcoI C'CATG_G
Cuts at: 513 1065 2143 513
Size: 552 1078 5031
Fragments arranged by size:
5031 1078 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6661
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6661
NotI GC'GGCC_GC
Cuts at: 4108 4108
Size: 6661
NspI r_CATG'y
Cuts at: 1132 1229 2199 4019 5564 1132
Size: 97 970 1820 1545 2229
Fragments arranged by size:
2229 1820 1545 970 97
PasI CC'CwG_GG
Cuts at: 1348 2383 1348
Size: 1035 5626
PciI A'CATG_T
Cuts at: 2195 5560 2195
Size: 3365 3296
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3739 1108
Size: 2631 4030
PfoI T'CCnGG_A
Cuts at: 2499 3937 2499
Size: 1438 5223
PmeI GTTT'AAAC
Cuts at: 3965 3965
Size: 6661
PmlI CAC'GTG
Cuts at: 2881 2980 2881
Size: 99 6562
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4730 4762 6243 6275 4730
Size: 32 1481 32 5116
Fragments arranged by size:
5116 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2702 3902 2702
Size: 1200 5461
PshAI GACnn'nnGTC
Cuts at: 1613 3362 1613
Size: 1749 4912
PsiI TTA'TAA
Cuts at: 4231 4231
Size: 6661
PstI C_TGCA'G
Cuts at: 830 3186 3454 4013 830
Size: 2356 268 559 3478
Fragments arranged by size:
3478 2356 559 268
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6661
PvuII CAG'CTG
Cuts at: 3728 3728
Size: 6661
SalI G'TCGA_C
Cuts at: 1936 4003 1936
Size: 2067 4594
SapI GCTCTTCn'nnn_
Cuts at: 2921 3683 2921
Size: 762 5899
SbfI CC_TGCA'GG
Cuts at: 4013 4013
Size: 6661
ScaI AGT'ACT
Cuts at: 4909 6621 4909
Size: 1712 4949
SfcI C'TryA_G
Cuts at: 826 1045 2227 3182 3450 3713 4009 5144 5825
Size: 219 1182 955 268 263 296 1135 681
Cuts at: 5825 6016 826
Size: 191 1471
Fragments arranged by size:
1471 1182 1135 955 681 296 268 263
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6661
SgrAI Cr'CCGG_yG
Cuts at: 1700 3272 1700
Size: 1572 5089
SmaI CCC'GGG
Cuts at: 1872 3988 1872
Size: 2116 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2592 3373 4765 5666 5928 820
Size: 197 926 649 781 1392 901 262 1553
Fragments arranged by size:
1553 1392 926 901 781 649 262 197
SnaBI TAC'GTA
Cuts at: 493 4452 493
Size: 3959 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6661
SphI G_CATG'C
Cuts at: 1132 4019 1132
Size: 2887 3774
SspI AAT'ATT
Cuts at: 5 52 4585 5
Size: 47 4533 2081
Fragments arranged by size:
4533 2081 47
StuI AGG'CCT
Cuts at: 1517 2031 1517
Size: 514 6147
StyI C'CwwG_G
Cuts at: 513 1065 2143 2187 3249 3529 3772 4100 513
Size: 552 1078 44 1062 280 243 328 3074
Fragments arranged by size:
3074 1078 1062 552 328 280 243 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4688 4705 4858 5043 5488 325
Size: 1255 3108 17 153 185 445 1498
Fragments arranged by size:
3108 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2216 4907 6619
Size: 274 80 33 51 1443 239 2691 1712
Cuts at: 6619 96
Size: 138
Fragments arranged by size:
2691 1712 1443 274 239 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2686 2718 6621 6653 2686
Size: 32 3903 32 2694
Fragments arranged by size:
3903 2694 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6661
XbaI T'CTAG_A
Cuts at: 2521 3997 2521
Size: 1476 5185
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6661
XmaI C'CCGG_G
Cuts at: 1870 3986 1870
Size: 2116 4545
XmnI GAAnn'nnTTC
Cuts at: 2174 3604 4790 2174
Size: 1430 1186 4045
Fragments arranged by size:
4045 1430 1186
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4469 276
Size: 53 83 186 936 2935 2468
Fragments arranged by size:
2935 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI