Restriction map for
pFN21AE9450
(Circular) (Six-base) MAPSORT of: pFN21AE9450.seq Check: 3873 from: 1 to: 9457
pFN21AE9450 9457 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2873 3119 3317 5874 2873
Size: 246 198 2557 6456
Fragments arranged by size:
6456 2557 246 198
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4257 7267 278
Size: 53 83 186 936 2721 3010 2468
Fragments arranged by size:
3010 2721 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6800 1937
Size: 4863 4594
Acc65I G'GTAC_C
Cuts at: 6778 6778
Size: 9457
AclI AA'CG_TT
Cuts at: 1563 7584 7957 1563
Size: 6021 373 3063
Fragments arranged by size:
6021 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5408 5783 5816 7520 8904 9172 1632
Size: 3776 375 33 1704 1384 268 1917
Fragments arranged by size:
3776 1917 1704 1384 375 268 33
AfeI AGC'GCT
Cuts at: 5159 5159
Size: 9457
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9260
AflIII A'CryG_T
Cuts at: 1203 2586 3476 3614 4626 4911 5850 6486 8356
Size: 1383 890 138 1012 285 939 636 1870
Cuts at: 8356 9333 1203
Size: 977 1327
Fragments arranged by size:
1870 1383 1327 1012 977 939 890 636
285 138
AgeI A'CCGG_T
Cuts at: 3059 8288 3059
Size: 5229 4228
AhdI GACnn_n'nnGTC
Cuts at: 4054 8186 4054
Size: 4132 5325
AleI CACnn'nnGTG
Cuts at: 1166 2141 5042 6632 1166
Size: 975 2901 1590 3991
Fragments arranged by size:
3991 2901 1590 975
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2885 1184
Size: 1701 7756
AlwNI CAG_nnn'CTG
Cuts at: 2431 2569 2983 3111 3199 3276 4009 6280 6499
Size: 138 414 128 88 77 733 2271 219
Cuts at: 6499 7200 8772 2431
Size: 701 1572 3116
Fragments arranged by size:
3116 2271 1572 733 701 414 219 138
128 88 77
ApaI G_GGCC'C
Cuts at: 2033 2118 2124 2498 3350 3660 3689 4211 4713
Size: 85 6 374 852 310 29 522 502
Cuts at: 4713 5898 6013 6213 6369 2033
Size: 1185 115 200 156 5121
Fragments arranged by size:
5121 1185 852 522 502 374 310 200
156 115 85 29 6
ApaLI G'TGCA_C
Cuts at: 5035 5552 7514 8670 5035
Size: 517 1962 1156 5822
Fragments arranged by size:
5822 1962 1156 517
ApoI r'AATT_y
Cuts at: 1493 4549 6766 6992 7144 9253 1493
Size: 3056 2217 226 152 2109 1697
Fragments arranged by size:
3056 2217 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 8011 160
Size: 872 6979 1606
Fragments arranged by size:
6979 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9457
AvaI C'yCGr_G
Cuts at: 1870 1943 2026 2117 2123 2327 2478 3537 3728
Size: 73 83 91 6 204 151 1059 191
Cuts at: 3728 3942 4706 5780 6155 6215 6329 6782 1870
Size: 214 764 1074 375 60 114 453 4545
Fragments arranged by size:
4545 1074 1059 764 453 375 214 204
191 151 114 91 83 73 60 6
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4662 4695 4662
Size: 33 9424
BamHI G'GATC_C
Cuts at: 2040 5334 6325 6787 7154 2040
Size: 3294 991 462 367 4343
Fragments arranged by size:
4343 3294 991 462 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2269 2442 2780 3233 3713
Size: 325 217 627 482 173 338 453 480
Cuts at: 3713 4402 4487 4637 4738 5944 6220 6307 6477
Size: 689 85 150 101 1206 276 87 170
Cuts at: 6477 6704 6778 8233 618
Size: 227 74 1455 1842
Fragments arranged by size:
1842 1455 1206 689 627 482 480 453
338 325 276 227 217 173 170 150
101 87 85 74
BanII G_rGCy'C
Cuts at: 1391 2033 2118 2124 2472 2498 3350 3660 3689
Size: 642 85 6 348 26 852 310 29
Cuts at: 3689 3735 3888 4211 4713 5184 5420 5642 5898
Size: 46 153 323 502 471 236 222 256
Cuts at: 5898 6013 6093 6213 6369 6410 6776 1391
Size: 115 80 120 156 41 366 4072
Fragments arranged by size:
4072 852 642 502 471 366 348 323
310 256 236 222 156 153 120 115
85 80 46 41 29 26 6
BbeI G_GCGC'C
Cuts at: 2273 2446 2784 5948 6708 2273
Size: 173 338 3164 760 5022
Fragments arranged by size:
5022 3164 760 338 173
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4429 5509 928
Size: 971 2530 1080 4876
Fragments arranged by size:
4876 2530 1080 971
BbvCI CC'TCA_GC
Cuts at: 6143 6143
Size: 9457
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7648 7682 1903
Size: 34 5711 34 3678
Fragments arranged by size:
5711 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4667 5570 7349 8559 4667
Size: 903 1779 1210 5565
Fragments arranged by size:
5565 1779 1210 903
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9457
BfrBI ATG'CAT
Cuts at: 4289 4289
Size: 9457
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2655 4202 6158
Size: 107 122 71 631 605 983 1547 1956
Cuts at: 6158 8068 136
Size: 1910 1525
Fragments arranged by size:
1956 1910 1547 1525 983 631 605 122
107 71
BglII A'GATC_T
Cuts at: 3741 4017 9452 3741
Size: 276 5435 3746
Fragments arranged by size:
5435 3746 276
BlpI GC'TnA_GC
Cuts at: 3963 4808 6002 6874 3963
Size: 845 1194 872 6546
Fragments arranged by size:
6546 1194 872 845
Bme1580I G_kGCm'C
Cuts at: 1790 2033 2118 2124 2280 2498 2778 3224 3350
Size: 243 85 6 156 218 280 446 126
Cuts at: 3350 3389 3563 3660 3689 4211 4490 4713 4743
Size: 39 174 97 29 522 279 223 30
Cuts at: 4743 5039 5556 5759 5898 6013 6213 6369 7518
Size: 296 517 203 139 115 200 156 1149
Cuts at: 7518 8674 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1149 522 517 446 296 280
279 243 223 218 203 200 174 156
156 139 126 115 97 85 39 30
29 6
BmgBI CAC'GTC
Cuts at: 1318 5689 1318
Size: 4371 5086
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3218 3316 3535
Size: 416 53 83 399 24 1801 98 219
Cuts at: 3535 3557 3889 4402 7188 8146 442
Size: 22 332 513 2786 958 1753
Fragments arranged by size:
2786 1801 1753 958 513 416 399 332
219 98 83 53 24 22
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9457
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4006 4006
Size: 9457
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2831 2933 3088 3253 3277 3451 5285 5549
Size: 1592 102 155 165 24 174 1834 264
Cuts at: 5549 6320 6434 6668 6692 6728 8117 1239
Size: 771 114 234 24 36 1389 2579
Fragments arranged by size:
2579 1834 1592 1389 771 264 234 174
165 155 114 102 36 24 24
Bpu10I CC'TnA_GC
Cuts at: 4151 6143 4151
Size: 1992 7465
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2731 5915 7582 8447 8745 2731
Size: 3184 1667 865 298 3443
Fragments arranged by size:
3443 3184 1667 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3527 3776 3994 8120 882
Size: 617 2028 249 218 4126 2219
Fragments arranged by size:
4126 2219 2028 617 249 218
BsaAI yAC'GTr
Cuts at: 493 1204 3615 4912 6040 6630 7248 493
Size: 711 2411 1297 1128 590 618 2702
Fragments arranged by size:
2702 2411 1297 1128 711 618 590
BsaBI GATnn'nnATC
Cuts at: 9451 9451
Size: 9457
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2080 2270 2443 2781
Size: 53 83 186 936 547 190 173 338
Cuts at: 2781 3590 4254 4749 5945 6578 6623 6705 7264
Size: 809 664 495 1196 633 45 82 559
Cuts at: 7264 7646 8281 275
Size: 382 635 1451
Fragments arranged by size:
1451 1196 936 809 664 635 633 559
547 495 382 338 190 186 173 83
82 53 45
BsaWI w'CCGG_w
Cuts at: 3059 3810 4095 4755 5136 6504 6606 7890 8288
Size: 751 285 660 381 1368 102 1284 398
Cuts at: 8288 8562 8709 9120 3059
Size: 274 147 411 3396
Fragments arranged by size:
3396 1368 1284 751 660 411 398 381
285 274 147 102
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2495 2525 4601 4631 4766
Size: 30 165 30 1092 30 2076 30 135
Cuts at: 4766 4796 5471 5501 6080 6110 1178
Size: 30 675 30 579 30 4525
Fragments arranged by size:
4525 2076 1092 675 579 165 135 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2930 3026 3092 3145 4184 4205 4675 4678
Size: 1744 96 66 53 1039 21 470 3
Cuts at: 4678 4972 5630 5798 5801 6101 1186
Size: 294 658 168 3 300 4542
Fragments arranged by size:
4542 1744 1039 658 470 300 294 168
96 66 53 21 3 3
BseYI C'CCAG_C
Cuts at: 2074 2457 2546 2954 3016 3046 3067 3717 3787
Size: 383 89 408 62 30 21 650 70
Cuts at: 3787 4197 4333 4803 5085 5175 5661 6200 6231
Size: 410 136 470 282 90 486 539 31
Cuts at: 6231 6270 6314 6356 6368 6544 8660 9156 2074
Size: 39 44 42 12 176 2116 496 2375
Fragments arranged by size:
2375 2116 650 539 496 486 470 410
408 383 282 176 136 90 89 70
62 44 42 39 31 30 21 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2165 3193 5042 5172 5846 5852 1163
Size: 573 429 1028 1849 130 674 6 4768
Fragments arranged by size:
4768 1849 1028 674 573 429 130 6
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2097 2178 2296 4542 5482 5771
Size: 275 186 96 81 118 2246 940 289
Cuts at: 5771 6907 7668 8696 1540
Size: 1136 761 1028 2301
Fragments arranged by size:
2301 2246 1136 1028 940 761 289 275
186 118 96 81
BsiHKAI G_wGCw'C
Cuts at: 3104 3951 4497 5039 5094 5420 5469 5556 6410
Size: 847 546 542 55 326 49 87 854
Cuts at: 6410 7518 7603 8674 3104
Size: 1108 85 1071 3887
Fragments arranged by size:
3887 1108 1071 854 847 546 542 326
87 85 55 49
BsmI GAATG_Cn'
Cuts at: 2825 3488 6968 7061 2825
Size: 663 3480 93 5221
Fragments arranged by size:
5221 3480 663 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4664 5778 4664
Size: 1114 8343
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2033 2118 2124 2280 2472 2498 2778
Size: 399 243 85 6 156 192 26 280
Cuts at: 2778 3104 3224 3350 3389 3563 3660 3689 3735
Size: 326 120 126 39 174 97 29 46
Cuts at: 3735 3888 3951 4211 4490 4497 4713 4743 5039
Size: 153 63 260 279 7 216 30 296
Cuts at: 5039 5094 5184 5420 5469 5556 5642 5759 5898
Size: 55 90 236 49 87 86 117 139
Cuts at: 5898 6013 6093 6213 6369 6410 6776 7518 7603
Size: 115 80 120 156 41 366 742 85
Cuts at: 7603 8674 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 366 326 296 280
279 260 243 236 216 192 174 156
156 153 139 126 120 120 117 115
97 90 87 86 85 85 80 63
55 49 46 41 39 30 29 26
7 6
BspEI T'CCGG_A
Cuts at: 4095 4755 5136 6606 9120 4095
Size: 660 381 1470 2514 4432
Fragments arranged by size:
4432 2514 1470 660 381
BspHI T'CATG_A
Cuts at: 5667 7346 9076 5667
Size: 1679 1730 6048
Fragments arranged by size:
6048 1730 1679
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2873 3119 3317 4059 5305 5874 6812 844
Size: 2029 246 198 742 1246 569 938 3489
Fragments arranged by size:
3489 2029 1246 938 742 569 246 198
BsrBI CCG'CTC
Cuts at: 1394 1823 5395 5487 6418 7344 1394
Size: 429 3572 92 931 926 3507
Fragments arranged by size:
3572 3507 931 926 429 92
BsrDI GCAATG_nn'
Cuts at: 65 1248 3375 7952 8126 65
Size: 1183 2127 4577 174 1396
Fragments arranged by size:
4577 2127 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2597 3059 3702 4776 5607 5842 6304
Size: 110 787 462 643 1074 831 235 462
Cuts at: 6304 8101 8288 1700
Size: 1797 187 2869
Fragments arranged by size:
2869 1797 1074 831 787 643 462 462
235 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9457
BssHII G'CGCG_C
Cuts at: 1925 2085 1925
Size: 160 9297
BssSI C'ACGA_G
Cuts at: 5354 6031 7517 8529 5354
Size: 677 1486 1012 6282
Fragments arranged by size:
6282 1486 1012 677
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2431 3171 6227 6280 794
Size: 1637 740 3056 53 3971
Fragments arranged by size:
3971 3056 1637 740 53
BstBI TT'CG_AA
Cuts at: 8261 8261
Size: 9457
BstEII G'GTnAC_C
Cuts at: 1184 3062 1184
Size: 1878 7579
BstXI CCAn_nnnn'nTGG
Cuts at: 5209 5209
Size: 9457
BstYI r'GATC_y
Cuts at: 1972 2040 3741 4017 4146 5334 5502 5692 6325
Size: 68 1701 276 129 1188 168 190 633
Cuts at: 6325 6787 7154 7539 7556 9008 9094 9106 9452
Size: 462 367 385 17 1452 86 12 346
Cuts at: 9452 1972
Size: 1977
Fragments arranged by size:
1977 1701 1452 1188 633 462 385 367
346 276 190 168 129 86 68 17
12
Bsu36I CC'TnA_GG
Cuts at: 4073 4280 4073
Size: 207 9250
BtgI C'CryG_G
Cuts at: 513 1065 2172 2234 2514 2676 2695 3089 3283
Size: 552 1107 62 280 162 19 394 194
Cuts at: 3283 4383 4443 6051 6057 6616 513
Size: 1100 60 1608 6 559 3354
Fragments arranged by size:
3354 1608 1107 1100 559 552 394 280
194 162 62 60 19 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3856 5710 487
Size: 683 56 2630 1854 4234
Fragments arranged by size:
4234 2630 1854 683 56
BtsI GCAGTG_nn'
Cuts at: 3176 3405 3563 3713 5739 6973 7767 7787 3176
Size: 229 158 150 2026 1234 794 20 4846
Fragments arranged by size:
4846 2026 1234 794 229 158 150 20
ClaI AT'CG_AT
Cuts at: 1554 4065 1554
Size: 2511 6946
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9422
DraI TTT'AAA
Cuts at: 6761 6761
Size: 9457
DraIII CAC_nnn'GTG
Cuts at: 3171 3529 3171
Size: 358 9099
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4843 5455 8464 809
Size: 507 3527 612 3009 1802
Fragments arranged by size:
3527 3009 1802 612 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2094 2175 2190
Size: 54 1425 50 275 18 264 81 15
Cuts at: 2190 2262 2293 2595 3280 3391 3954 5046 5199
Size: 72 31 302 685 111 563 1092 153
Cuts at: 5199 5653 5768 5844 6054 6060 6904 7793 9422
Size: 454 115 76 210 6 844 889 1629
Cuts at: 9422 8
Size: 43
Fragments arranged by size:
1629 1425 1092 889 844 685 563 454
302 275 264 210 153 115 111 81
76 72 54 50 43 31 18 15
6
EagI C'GGCC_G
Cuts at: 1537 1812 2094 2175 2293 5768 6904 1537
Size: 275 282 81 118 3475 1136 4090
Fragments arranged by size:
4090 3475 1136 282 275 118 81
EarI CTCTTCn'nnn_
Cuts at: 1353 5501 5513 7387 1353
Size: 4148 12 1874 3423
Fragments arranged by size:
4148 3423 1874 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3433 4681 5479 6237 6436 8044
Size: 1064 138 1844 1248 798 758 199 1608
Cuts at: 8044 8418 8564 387
Size: 374 146 1280
Fragments arranged by size:
1844 1608 1280 1248 1064 798 758 374
199 146 138
EcoICRI GAG'CTC
Cuts at: 5418 6408 5418
Size: 990 8467
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2831 2933 3088 3253 3277 3451 5285
Size: 393 1199 102 155 165 24 174 1834
Cuts at: 5285 5408 5549 5783 5816 6320 6434 6668 6692
Size: 123 141 234 33 504 114 234 24
Cuts at: 6692 6728 7520 8117 8904 9172 1239
Size: 36 792 597 787 268 1524
Fragments arranged by size:
1834 1524 1199 792 787 597 504 393
268 234 234 174 165 155 141 123
114 102 36 33 24 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2759 3956 2759
Size: 1197 8260
EcoO109I rG'GnC_Cy
Cuts at: 2030 2114 2495 2681 3315 3346 3347 3657 3685
Size: 84 381 186 634 31 1 310 28
Cuts at: 3685 3686 4048 4208 4276 4710 5294 5403 5895
Size: 1 362 160 68 434 584 109 492
Cuts at: 5895 6210 6353 6365 6496 6591 2030
Size: 315 143 12 131 95 4896
Fragments arranged by size:
4896 634 584 492 434 381 362 315
310 186 160 143 131 109 95 84
68 31 28 12 1 1
EcoRI G'AATT_C
Cuts at: 4549 6766 7144 4549
Size: 2217 378 6862
Fragments arranged by size:
6862 2217 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4453 5974 4453
Size: 1521 7936
FspI TGC'GCA
Cuts at: 2303 4598 7963 2303
Size: 2295 3365 3797
Fragments arranged by size:
3797 3365 2295
FspAI rTGC'GCAy
Cuts at: 2303 2303
Size: 9457
HaeII r_GCGC'y
Cuts at: 1678 2242 2273 2446 2784 3438 4815 5161 5447
Size: 564 31 173 338 654 1377 346 286
Cuts at: 5447 5948 6708 8604 1678
Size: 501 760 1896 2531
Fragments arranged by size:
2531 1896 1377 760 654 564 501 346
338 286 173 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2495 2527 3068 3100 3233
Size: 32 135 32 923 32 541 32 133
Cuts at: 3233 3265 4040 4072 4454 4486 4667 4699 4766
Size: 32 775 32 382 32 181 32 67
Cuts at: 4766 4798 5471 5503 8098 8130 8172 8204 1373
Size: 32 673 32 2595 32 42 32 2626
Fragments arranged by size:
2626 2595 923 775 673 541 382 181
135 133 67 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5605 6801 7047 669
Size: 988 281 3667 1196 246 3079
Fragments arranged by size:
3667 3079 1196 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9457
HpaI GTT'AAC
Cuts at: 7047 7047
Size: 9457
KasI G'GCGC_C
Cuts at: 2269 2442 2780 5944 6704 2269
Size: 173 338 3164 760 5022
Fragments arranged by size:
5022 3164 760 338 173
KpnI G_GTAC'C
Cuts at: 6782 6782
Size: 9457
MfeI C'AATT_G
Cuts at: 7056 7056
Size: 9457
MluI A'CGCG_T
Cuts at: 2586 3476 9333 2586
Size: 890 5857 2710
Fragments arranged by size:
5857 2710 890
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 8571 8755 1305
Size: 7266 184 2007
Fragments arranged by size:
7266 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2264 3956 5201 10
Size: 54 1425 343 432 1692 1245 4266
Fragments arranged by size:
4266 1692 1425 1245 432 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2141 2258 3932 4910 5042 5120 5207
Size: 648 975 117 1674 978 132 78 87
Cuts at: 5207 5546 6632 7415 7774 7933 518
Size: 339 1086 783 359 159 2042
Fragments arranged by size:
2042 1674 1086 978 975 783 648 359
339 159 132 117 87 78
MspA1I CmG'CkG
Cuts at: 1606 2053 2174 2236 2286 2577 2678 2697 2954
Size: 447 121 62 50 291 101 19 257
Cuts at: 2954 3177 3196 3450 3721 3791 3903 4201 4341
Size: 223 19 254 271 70 112 298 140
Cuts at: 4341 5303 5437 6204 6524 6655 6670 6718 6873
Size: 962 134 767 320 131 15 48 155
Cuts at: 6873 7550 8698 8943 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 962 767 677 447 320 298
291 271 257 254 245 223 155 140
134 131 121 112 101 70 62 50
48 19 19 15
NaeI GCC'GGC
Cuts at: 5844 6306 5844
Size: 462 8995
NarI GG'CG_CC
Cuts at: 2270 2443 2781 5945 6705 2270
Size: 173 338 3164 760 5022
Fragments arranged by size:
5022 3164 760 338 173
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 8905
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9457
NgoMIV G'CCGG_C
Cuts at: 5842 6304 5842
Size: 462 8995
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9457
NotI GC'GGCC_GC
Cuts at: 2094 2175 6904 2094
Size: 81 4729 4647
Fragments arranged by size:
4729 4647 81
NsiI A_TGCA'T
Cuts at: 4291 4291
Size: 9457
NspI r_CATG'y
Cuts at: 1132 1229 4630 6490 6815 8360 1132
Size: 97 3401 1860 325 1545 2229
Fragments arranged by size:
3401 2229 1860 1545 325 97
PasI CC'CwG_GG
Cuts at: 1348 3457 4121 6594 1348
Size: 2109 664 2473 4211
Fragments arranged by size:
4211 2473 2109 664
PciI A'CATG_T
Cuts at: 4626 6486 8356 4626
Size: 1860 1870 5727
Fragments arranged by size:
5727 1870 1860
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3230 3529 3569 4009 1108
Size: 2122 299 40 440 6556
Fragments arranged by size:
6556 2122 440 299 40
PfoI T'CCnGG_A
Cuts at: 2210 2969 5577 6349 6492 2210
Size: 759 2608 772 143 5175
Fragments arranged by size:
5175 2608 772 759 143
PmeI GTTT'AAAC
Cuts at: 6761 6761
Size: 9457
PmlI CAC'GTG
Cuts at: 3615 6630 3615
Size: 3015 6442
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7526 7558 9039 9071 7526
Size: 32 1481 32 7912
Fragments arranged by size:
7912 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2681 4048 4276 5294 6353 6496 2681
Size: 1367 228 1018 1059 143 5642
Fragments arranged by size:
5642 1367 1059 1018 228 143
PshAI GACnn'nnGTC
Cuts at: 1613 2589 6752 1613
Size: 976 4163 4318
Fragments arranged by size:
4318 4163 976
PsiI TTA'TAA
Cuts at: 7027 7027
Size: 9457
PspOMI G'GGCC_C
Cuts at: 2029 2114 2120 2494 3346 3656 3685 4207 4709
Size: 85 6 374 852 310 29 522 502
Cuts at: 4709 5894 6009 6209 6365 2029
Size: 1185 115 200 156 5121
Fragments arranged by size:
5121 1185 852 522 502 374 310 200
156 115 85 29 6
PspXI vC'TCGA_Gb
Cuts at: 2327 2327
Size: 9457
PstI C_TGCA'G
Cuts at: 830 2285 2415 2436 3033 3314 3555 4530 6516
Size: 1455 130 21 597 281 241 975 1986
Cuts at: 6516 6660 6809 830
Size: 144 149 3478
Fragments arranged by size:
3478 1986 1455 975 597 281 241 149
144 130 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9457
PvuII CAG'CTG
Cuts at: 3450 4201 3450
Size: 751 8706
SacI G_AGCT'C
Cuts at: 5420 6410 5420
Size: 990 8467
SacII CC_GC'GG
Cuts at: 2175 2237 2679 2698 2175
Size: 62 442 19 8934
Fragments arranged by size:
8934 442 62 19
SalI G'TCGA_C
Cuts at: 1936 6799 1936
Size: 4863 4594
SanDI GG'GwC_CC
Cuts at: 2681 6353 2681
Size: 3672 5785
SbfI CC_TGCA'GG
Cuts at: 2415 3314 6809 2415
Size: 899 3495 5063
Fragments arranged by size:
5063 3495 899
ScaI AGT'ACT
Cuts at: 7705 9417 7705
Size: 1712 7745
SexAI A'CCwGG_T
Cuts at: 2523 4293 4308 2523
Size: 1770 15 7672
Fragments arranged by size:
7672 1770 15
SfcI C'TryA_G
Cuts at: 826 1045 2281 2411 2432 3029 3160 3310 3551
Size: 219 1236 130 21 597 131 150 241
Cuts at: 3551 3919 4039 4108 4498 4526 5701 6512 6656
Size: 368 120 69 390 28 1175 811 144
Cuts at: 6656 6805 7940 8621 8812 826
Size: 149 1135 681 191 1471
Fragments arranged by size:
1471 1236 1175 1135 811 681 597 390
368 241 219 191 150 149 144 131
130 120 69 28 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 6158 6158
Size: 9457
SfoI GGC'GCC
Cuts at: 2271 2444 2782 5946 6706 2271
Size: 173 338 3164 760 5022
Fragments arranged by size:
5022 3164 760 338 173
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9457
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9457
SmaI CCC'GGG
Cuts at: 1872 2028 2119 2480 3944 6331 6784 1872
Size: 156 91 361 1464 2387 453 4545
Fragments arranged by size:
4545 2387 1464 453 361 156 91
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2327 2710 5930 7561 8462 8724
Size: 197 926 384 383 3220 1631 901 262
Cuts at: 8724 820
Size: 1553
Fragments arranged by size:
3220 1631 1553 926 901 384 383 262
197
SnaBI TAC'GTA
Cuts at: 493 7248 493
Size: 6755 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9457
SphI G_CATG'C
Cuts at: 1132 6815 1132
Size: 5683 3774
SrfI GCCC'GGGC
Cuts at: 2119 2119
Size: 9457
SspI AAT'ATT
Cuts at: 5 52 7381 5
Size: 47 7329 2081
Fragments arranged by size:
7329 2081 47
StuI AGG'CCT
Cuts at: 1517 2844 2942 3584 5102 1517
Size: 1327 98 642 1518 5872
Fragments arranged by size:
5872 1518 1327 642 98
StyI C'CwwG_G
Cuts at: 513 1065 2265 3957 4218 4398 4605 5220 5811
Size: 552 1200 1692 261 180 207 615 591
Cuts at: 5811 6081 6896 513
Size: 270 815 3074
Fragments arranged by size:
3074 1692 1200 815 615 591 552 270
261 207 180
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3517 3731 5077 5687 6622 6764 7484
Size: 1255 1937 214 1346 610 935 142 720
Cuts at: 7484 7501 7654 7839 8284 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1937 1498 1346 1255 935 720 610 445
214 185 153 142 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6096 7703 9415
Size: 274 80 33 51 1443 4119 1607 1712
Cuts at: 9415 96
Size: 138
Fragments arranged by size:
4119 1712 1607 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3560 3592 9417 9449 3560
Size: 32 5825 32 3568
Fragments arranged by size:
5825 3568 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4415 1316
Size: 3099 6358
XbaI T'CTAG_A
Cuts at: 6793 6793
Size: 9457
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4631 6715 4631
Size: 2084 7373
XhoI C'TCGA_G
Cuts at: 1943 2327 1943
Size: 384 9073
XmaI C'CCGG_G
Cuts at: 1870 2026 2117 2478 3942 6329 6782 1870
Size: 156 91 361 1464 2387 453 4545
Fragments arranged by size:
4545 2387 1464 453 361 156 91
XmnI GAAnn'nnTTC
Cuts at: 7586 7586
Size: 9457
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4255 7265 276
Size: 53 83 186 936 2721 3010 2468
Fragments arranged by size:
3010 2721 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SacII SalI SanDI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BsiWI BstZ17I EcoRV FseI NruI
PacI PsrI RsrII SapI SwaI