Restriction map for
pFN21AE9449
(Circular) (Six-base) MAPSORT of: pFN21AE9449.seq Check: 9367 from: 1 to: 5767
pFN21AE9449 5767 bp
With 182 enzymes: SgfI *
March 28, 2012 12:11 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3577 278
Size: 53 83 186 936 2041 2468
Fragments arranged by size:
2468 2041 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3110 1937
Size: 1173 4594
Acc65I G'GTAC_C
Cuts at: 3088 3088
Size: 5767
AclI AA'CG_TT
Cuts at: 1563 3894 4267 1563
Size: 2331 373 3063
Fragments arranged by size:
3063 2331 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2983 3830 5214 5482 1632
Size: 1351 847 1384 268 1917
Fragments arranged by size:
1917 1384 1351 847 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5570
AflIII A'CryG_T
Cuts at: 1203 2172 4666 5643 1203
Size: 969 2494 977 1327
Fragments arranged by size:
2494 1327 977 969
AgeI A'CCGG_T
Cuts at: 4598 4598
Size: 5767
AhdI GACnn_n'nnGTC
Cuts at: 4496 4496
Size: 5767
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5767
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2753 1184
Size: 1569 4198
AlwNI CAG_nnn'CTG
Cuts at: 3510 5082 3510
Size: 1572 4195
ApaLI G'TGCA_C
Cuts at: 2551 3824 4980 2551
Size: 1273 1156 3338
Fragments arranged by size:
3338 1273 1156
ApoI r'AATT_y
Cuts at: 1493 2939 3048 3076 3302 3454 5563 1493
Size: 1446 109 28 226 152 2109 1697
Fragments arranged by size:
2109 1697 1446 226 152 109 28
AseI AT'TA_AT
Cuts at: 160 1032 4321 160
Size: 872 3289 1606
Fragments arranged by size:
3289 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5767
AvaI C'yCGr_G
Cuts at: 1870 1943 3092 1870
Size: 73 1149 4545
Fragments arranged by size:
4545 1149 73
BamHI G'GATC_C
Cuts at: 2068 2721 3097 3464 2068
Size: 653 376 367 4371
Fragments arranged by size:
4371 653 376 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2872 3088 4543 618
Size: 325 217 627 1085 216 1455 1842
Fragments arranged by size:
1842 1455 1085 627 325 217 216
BanII G_rGCy'C
Cuts at: 1391 2538 3086 1391
Size: 1147 548 4072
Fragments arranged by size:
4072 1147 548
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2169 3024 928
Size: 971 270 855 3671
Fragments arranged by size:
3671 971 855 270
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3958 3992 1903
Size: 34 2021 34 3678
Fragments arranged by size:
3678 2021 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3659 4869 3659
Size: 1210 4557
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5767
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2474 4378 136
Size: 107 122 71 631 605 802 1904 1525
Fragments arranged by size:
1904 1525 802 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5762 5762
Size: 5767
BlpI GC'TnA_GC
Cuts at: 3184 3184
Size: 5767
Bme1580I G_kGCm'C
Cuts at: 1790 2555 3828 4984 1790
Size: 765 1273 1156 2573
Fragments arranged by size:
2573 1273 1156 765
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5767
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3498 4456 442
Size: 416 53 83 399 24 2081 958 1753
Fragments arranged by size:
2081 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5767
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4427 1239
Size: 3188 2579
Bpu10I CC'TnA_GC
Cuts at: 2532 2532
Size: 5767
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3892 4757 5055 3892
Size: 865 298 4604
Fragments arranged by size:
4604 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2194 4430 882
Size: 617 695 2236 2219
Fragments arranged by size:
2236 2219 695 617
BsaAI yAC'GTr
Cuts at: 493 1204 2527 2662 3558 493
Size: 711 1323 135 896 2702
Fragments arranged by size:
2702 1323 896 711 135
BsaBI GATnn'nnATC
Cuts at: 2067 5761 2067
Size: 3694 2073
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3574 3956 4591 275
Size: 53 83 186 936 2041 382 635 1451
Fragments arranged by size:
2041 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4200 4598 4872 5019 5430 4200
Size: 398 274 147 411 4537
Fragments arranged by size:
4537 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5542
Fragments arranged by size:
5542 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5767
BseYI C'CCAG_C
Cuts at: 2298 4970 5466 2298
Size: 2672 496 2599
Fragments arranged by size:
2672 2599 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2990 1163
Size: 573 1254 3940
Fragments arranged by size:
3940 1254 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3217 3978 5006 1540
Size: 275 186 1216 761 1028 2301
Fragments arranged by size:
2301 1216 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2555 3828 3913 4984 2555
Size: 1273 85 1071 3338
Fragments arranged by size:
3338 1273 1071 85
BsmI GAATG_Cn'
Cuts at: 3278 3371 3278
Size: 93 5674
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2538 2555 3086 3828 3913 4984 1391
Size: 399 748 17 531 742 85 1071 2174
Fragments arranged by size:
2174 1071 748 742 531 399 85 17
BspEI T'CCGG_A
Cuts at: 5430 5430
Size: 5767
BspHI T'CATG_A
Cuts at: 3052 3656 5386 3052
Size: 604 1730 3433
Fragments arranged by size:
3433 1730 604
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3122 844
Size: 2278 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3654 1394
Size: 429 1831 3507
Fragments arranged by size:
3507 1831 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2670 4262 4436 65
Size: 1183 1422 1592 174 1396
Fragments arranged by size:
1592 1422 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4411 4598 1700
Size: 110 2601 187 2869
Fragments arranged by size:
2869 2601 187 110
BsrGI T'GTAC_A
Cuts at: 96 2904 96
Size: 2808 2959
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5767
BssSI C'ACGA_G
Cuts at: 2548 3827 4839 2548
Size: 1279 1012 3476
Fragments arranged by size:
3476 1279 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2894 794
Size: 2100 3667
BstBI TT'CG_AA
Cuts at: 4571 4571
Size: 5767
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5767
BstXI CCAn_nnnn'nTGG
Cuts at: 2506 2960 2506
Size: 454 5313
BstYI r'GATC_y
Cuts at: 1972 2068 2721 3097 3464 3849 3866 5318 5404
Size: 96 653 376 367 385 17 1452 86
Cuts at: 5404 5416 5762 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 653 385 376 367 346 96
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2031 2031
Size: 5767
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4276
Fragments arranged by size:
4276 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2780 2915 487
Size: 683 56 1554 135 3339
Fragments arranged by size:
3339 1554 683 135 56
BtsI GCAGTG_nn'
Cuts at: 2019 2817 3283 4077 4097 2019
Size: 798 466 794 20 3689
Fragments arranged by size:
3689 798 794 466 20
ClaI AT'CG_AT
Cuts at: 1554 2395 1554
Size: 841 4926
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5732
DraI TTT'AAA
Cuts at: 3071 3071
Size: 5767
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4774 809
Size: 507 3458 1802
Fragments arranged by size:
3458 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3214 4103 5732
Size: 54 1425 50 275 18 1384 889 1629
Cuts at: 5732 8
Size: 43
Fragments arranged by size:
1629 1425 1384 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3214 1537
Size: 275 1402 4090
Fragments arranged by size:
4090 1402 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2621 2695 3051 3697 1353
Size: 1268 74 356 646 3423
Fragments arranged by size:
3423 1268 646 356 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4354 4728 4874 387
Size: 1064 138 2765 374 146 1280
Fragments arranged by size:
2765 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2983 3830 4427 5214 5482 1239
Size: 393 1351 847 597 787 268 1524
Fragments arranged by size:
1524 1351 847 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2030 2030
Size: 5767
EcoRI G'AATT_C
Cuts at: 3076 3454 3076
Size: 378 5389
FspI TGC'GCA
Cuts at: 4273 4273
Size: 5767
HaeII r_GCGC'y
Cuts at: 1678 2044 4914 1678
Size: 366 2870 2531
Fragments arranged by size:
2870 2531 366
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2893 2925 4408 4440 4482
Size: 32 135 32 1321 32 1483 32 42
Cuts at: 4482 4514 1373
Size: 32 2626
Fragments arranged by size:
2626 1483 1321 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3111 3357 669
Size: 988 281 1173 246 3079
Fragments arranged by size:
3079 1173 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5767
HpaI GTT'AAC
Cuts at: 3357 3357
Size: 5767
KpnI G_GTAC'C
Cuts at: 3092 3092
Size: 5767
MfeI C'AATT_G
Cuts at: 3366 3366
Size: 5767
MluI A'CGCG_T
Cuts at: 5643 5643
Size: 5767
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2699 4881 5065 1305
Size: 1394 2182 184 2007
Fragments arranged by size:
2182 2007 1394 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3945
Fragments arranged by size:
3945 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3725 4084 4243 518
Size: 648 2559 359 159 2042
Fragments arranged by size:
2559 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2207 2298 2325 3183 3860 5008 5253 1606
Size: 601 91 27 858 677 1148 245 2120
Fragments arranged by size:
2120 1148 858 677 601 245 91 27
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4276
Fragments arranged by size:
4276 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5767
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5767
NotI GC'GGCC_GC
Cuts at: 3214 3214
Size: 5767
NspI r_CATG'y
Cuts at: 1132 1229 2176 3125 4670 1132
Size: 97 947 949 1545 2229
Fragments arranged by size:
2229 1545 949 947 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5767
PciI A'CATG_T
Cuts at: 2172 4666 2172
Size: 2494 3273
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2740 1108
Size: 1632 4135
PmeI GTTT'AAAC
Cuts at: 3071 3071
Size: 5767
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3836 3868 5349 5381 3836
Size: 32 1481 32 4222
Fragments arranged by size:
4222 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2381 1613
Size: 768 4999
PsiI TTA'TAA
Cuts at: 3337 3337
Size: 5767
PstI C_TGCA'G
Cuts at: 830 2899 3119 830
Size: 2069 220 3478
Fragments arranged by size:
3478 2069 220
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5767
SalI G'TCGA_C
Cuts at: 1936 3109 1936
Size: 1173 4594
SbfI CC_TGCA'GG
Cuts at: 3119 3119
Size: 5767
ScaI AGT'ACT
Cuts at: 4015 5727 4015
Size: 1712 4055
SfcI C'TryA_G
Cuts at: 826 1045 2895 3115 4250 4931 5122 826
Size: 219 1850 220 1135 681 191 1471
Fragments arranged by size:
1850 1471 1135 681 220 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5767
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5767
SmaI CCC'GGG
Cuts at: 1872 3094 1872
Size: 1222 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3871 4772 5034 820
Size: 197 926 1928 901 262 1553
Fragments arranged by size:
1928 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2527 2662 3558 493
Size: 2034 135 896 2702
Fragments arranged by size:
2702 2034 896 135
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5767
SphI G_CATG'C
Cuts at: 1132 3125 1132
Size: 1993 3774
SspI AAT'ATT
Cuts at: 5 52 3691 5
Size: 47 3639 2081
Fragments arranged by size:
3639 2081 47
StuI AGG'CCT
Cuts at: 1517 2579 1517
Size: 1062 4705
StyI C'CwwG_G
Cuts at: 513 1065 2004 2505 2676 3206 513
Size: 552 939 501 171 530 3074
Fragments arranged by size:
3074 939 552 530 501 171
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2290 3794 3811 3964 4149 4594 325
Size: 1255 710 1504 17 153 185 445 1498
Fragments arranged by size:
1504 1498 1255 710 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2904 4013 5725
Size: 274 80 33 51 1443 927 1109 1712
Cuts at: 5725 96
Size: 138
Fragments arranged by size:
1712 1443 1109 927 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5727 5759 5727
Size: 32 5735
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5767
XbaI T'CTAG_A
Cuts at: 2180 3103 2180
Size: 923 4844
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2503 2503
Size: 5767
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5767
XmaI C'CCGG_G
Cuts at: 1870 3092 1870
Size: 1222 4545
XmnI GAAnn'nnTTC
Cuts at: 3896 3896
Size: 5767
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3575 276
Size: 53 83 186 936 2041 2468
Fragments arranged by size:
2468 2041 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI