Restriction map for
pFN21AE9448
(Circular) (Six-base) MAPSORT of: pFN21AE9448.seq Check: 8491 from: 1 to: 6139
pFN21AE9448 6139 bp
With 182 enzymes: SgfI *
March 28, 2012 12:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2323 3105 2323
Size: 782 5357
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3949 278
Size: 53 83 186 936 2413 2468
Fragments arranged by size:
2468 2413 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3482 1937
Size: 1545 4594
Acc65I G'GTAC_C
Cuts at: 3460 3460
Size: 6139
AclI AA'CG_TT
Cuts at: 1563 4266 4639 1563
Size: 2703 373 3063
Fragments arranged by size:
3063 2703 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2764 4202 5586 5854 1632
Size: 1132 1438 1384 268 1917
Fragments arranged by size:
1917 1438 1384 1132 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5942
AflIII A'CryG_T
Cuts at: 1203 2322 2977 5038 6015 1203
Size: 1119 655 2061 977 1327
Fragments arranged by size:
2061 1327 1119 977 655
AgeI A'CCGG_T
Cuts at: 4970 4970
Size: 6139
AhdI GACnn_n'nnGTC
Cuts at: 4868 4868
Size: 6139
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6139
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6139
AlwNI CAG_nnn'CTG
Cuts at: 2153 2866 3097 3284 3337 3882 5454 2153
Size: 713 231 187 53 545 1572 2838
Fragments arranged by size:
2838 1572 713 545 231 187 53
ApaLI G'TGCA_C
Cuts at: 4196 5352 4196
Size: 1156 4983
ApoI r'AATT_y
Cuts at: 1493 3448 3674 3826 5935 1493
Size: 1955 226 152 2109 1697
Fragments arranged by size:
2109 1955 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4693 160
Size: 872 3661 1606
Fragments arranged by size:
3661 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6139
AvaI C'yCGr_G
Cuts at: 1870 1943 2645 3464 1870
Size: 73 702 819 4545
Fragments arranged by size:
4545 819 702 73
BamHI G'GATC_C
Cuts at: 3469 3836 3469
Size: 367 5772
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3004 3225 3358 3460 4915
Size: 325 217 627 1217 221 133 102 1455
Cuts at: 4915 618
Size: 1842
Fragments arranged by size:
1842 1455 1217 627 325 221 217 133
102
BanII G_rGCy'C
Cuts at: 1391 2055 2458 2809 2935 3002 3245 3458 1391
Size: 664 403 351 126 67 243 213 4072
Fragments arranged by size:
4072 664 403 351 243 213 126 67
BbeI G_GCGC'C
Cuts at: 3229 3229
Size: 6139
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2374 3407 928
Size: 971 475 1033 3660
Fragments arranged by size:
3660 1033 971 475
BbvCI CC'TCA_GC
Cuts at: 3203 3203
Size: 6139
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2423 2457 4330 4364 1903
Size: 34 486 34 1873 34 3678
Fragments arranged by size:
3678 1873 486 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4031 5241 4031
Size: 1210 4929
BclI T'GATC_A
Cuts at: 1548 2478 1548
Size: 930 5209
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2957 4750 136
Size: 107 122 71 631 605 1285 1793 1525
Fragments arranged by size:
1793 1525 1285 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6134 6134
Size: 6139
BlpI GC'TnA_GC
Cuts at: 3556 3556
Size: 6139
Bme1580I G_kGCm'C
Cuts at: 1790 2228 2646 4200 5356 1790
Size: 438 418 1554 1156 2573
Fragments arranged by size:
2573 1554 1156 438 418
BmgBI CAC'GTC
Cuts at: 1318 2980 1318
Size: 1662 4477
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3870 4828 442
Size: 416 53 83 399 24 2453 958 1753
Fragments arranged by size:
2453 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6139
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2098 2626 2650 2795 3359 3378 4799 1239
Size: 859 528 24 145 564 19 1421 2579
Fragments arranged by size:
2579 1421 859 564 528 145 24 19
Bpu10I CC'TnA_GC
Cuts at: 3203 3203
Size: 6139
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4264 5129 5427 4264
Size: 865 298 4976
Fragments arranged by size:
4976 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4802 882
Size: 617 3303 2219
Fragments arranged by size:
3303 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2641 3930 493
Size: 711 1437 1289 2702
Fragments arranged by size:
2702 1437 1289 711
BsaBI GATnn'nnATC
Cuts at: 6133 6133
Size: 6139
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2549 3226 3946 4328
Size: 53 83 186 936 1016 677 720 382
Cuts at: 4328 4963 275
Size: 635 1451
Fragments arranged by size:
1451 1016 936 720 677 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2307 4572 4970 5244 5391 5802 2307
Size: 2265 398 274 147 411 2644
Fragments arranged by size:
2644 2265 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2793 2823 1178
Size: 30 165 30 1390 30 4494
Fragments arranged by size:
4494 1390 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2903 1186
Size: 1717 4422
BseYI C'CCAG_C
Cuts at: 2036 2054 2071 2081 2260 2578 2966 3090 3118
Size: 18 17 10 179 318 388 124 28
Cuts at: 3118 3152 3300 3419 5342 5838 2036
Size: 34 148 119 1923 496 2337
Fragments arranged by size:
2337 1923 496 388 318 179 148 124
119 34 28 18 17 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5566
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2697 3589 4350 5378 1540
Size: 275 186 696 892 761 1028 2301
Fragments arranged by size:
2301 1028 892 761 696 275 186
BsiHKAI G_wGCw'C
Cuts at: 2458 2925 3002 4200 4285 5356 2458
Size: 467 77 1198 85 1071 3241
Fragments arranged by size:
3241 1198 1071 467 85 77
BsmI GAATG_Cn'
Cuts at: 3650 3743 3650
Size: 93 6046
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2055 2228 2458 2646 2809 2925 2935
Size: 399 265 173 230 188 163 116 10
Cuts at: 2935 3002 3245 3458 4200 4285 5356 1391
Size: 67 243 213 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 265 243 230 213
188 173 163 116 85 67 10
BspEI T'CCGG_A
Cuts at: 2307 5802 2307
Size: 3495 2644
BspHI T'CATG_A
Cuts at: 4028 5758 4028
Size: 1730 4409
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2323 3105 3494 844
Size: 1479 782 389 3489
Fragments arranged by size:
3489 1479 782 389
BsrBI CCG'CTC
Cuts at: 1394 1823 3162 3174 4026 1394
Size: 429 1339 12 852 3507
Fragments arranged by size:
3507 1339 852 429 12
BsrDI GCAATG_nn'
Cuts at: 65 1248 2842 3084 4634 4808 65
Size: 1183 1594 242 1550 174 1396
Fragments arranged by size:
1594 1550 1396 1183 242 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2124 2692 3132 4783 4970 1700
Size: 110 314 568 440 1651 187 2869
Fragments arranged by size:
2869 1651 568 440 314 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6139
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6139
BssSI C'ACGA_G
Cuts at: 4199 5211 4199
Size: 1012 5127
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2866 794
Size: 2072 4067
BstBI TT'CG_AA
Cuts at: 4943 4943
Size: 6139
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6139
BstXI CCAn_nnnn'nTGG
Cuts at: 2062 2085 2500 2062
Size: 23 415 5701
Fragments arranged by size:
5701 415 23
BstYI r'GATC_y
Cuts at: 1972 3210 3469 3836 4221 4238 5690 5776 5788
Size: 1238 259 367 385 17 1452 86 12
Cuts at: 5788 6134 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1238 385 367 346 259 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2187 513
Size: 552 1122 4465
Fragments arranged by size:
4465 1122 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2479 3001 487
Size: 683 56 1253 522 3625
Fragments arranged by size:
3625 1253 683 522 56
BtsI GCAGTG_nn'
Cuts at: 2414 3655 4449 4469 2414
Size: 1241 794 20 4084
Fragments arranged by size:
4084 1241 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6139
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6104
DraI TTT'AAA
Cuts at: 3443 3443
Size: 6139
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5146 809
Size: 507 3830 1802
Fragments arranged by size:
3830 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2064 2490 2532
Size: 54 1425 50 275 18 234 426 42
Cuts at: 2532 2694 2886 3081 3586 4475 6104 8
Size: 162 192 195 505 889 1629 43
Fragments arranged by size:
1629 1425 889 505 426 275 234 195
192 162 54 50 43 42 18
EagI C'GGCC_G
Cuts at: 1537 1812 2694 3586 1537
Size: 275 882 892 4090
Fragments arranged by size:
4090 892 882 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2921 4069 1353
Size: 1568 1148 3423
Fragments arranged by size:
3423 1568 1148
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4726 5100 5246 387
Size: 1064 138 3137 374 146 1280
Fragments arranged by size:
3137 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2456 3000 2456
Size: 544 5595
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2098 2626 2650 2764 2795 3359 3378
Size: 393 466 528 24 114 31 564 19
Cuts at: 3378 4202 4799 5586 5854 1239
Size: 824 597 787 268 1524
Fragments arranged by size:
1524 824 787 597 564 528 466 393
268 114 31 24 19
EcoNI CCTnn'n_nnAGG
Cuts at: 2290 2290
Size: 6139
EcoO109I rG'GnC_Cy
Cuts at: 2176 2374 2823 3427 2176
Size: 198 449 604 4888
Fragments arranged by size:
4888 604 449 198
EcoRI G'AATT_C
Cuts at: 3448 3826 3448
Size: 378 5761
FspI TGC'GCA
Cuts at: 4645 4645
Size: 6139
HaeII r_GCGC'y
Cuts at: 1678 2637 3229 5286 1678
Size: 959 592 2057 2531
Fragments arranged by size:
2531 2057 959 592
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2793 2825 4780 4812 4854
Size: 32 135 32 1221 32 1955 32 42
Cuts at: 4854 4886 1373
Size: 32 2626
Fragments arranged by size:
2626 1955 1221 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3483 3729 669
Size: 988 281 1545 246 3079
Fragments arranged by size:
3079 1545 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6139
HpaI GTT'AAC
Cuts at: 3729 3729
Size: 6139
KasI G'GCGC_C
Cuts at: 3225 3225
Size: 6139
KpnI G_GTAC'C
Cuts at: 3464 3464
Size: 6139
MfeI C'AATT_G
Cuts at: 3738 3738
Size: 6139
MluI A'CGCG_T
Cuts at: 6015 6015
Size: 6139
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3222 5253 5437 1305
Size: 1917 2031 184 2007
Fragments arranged by size:
2031 2007 1917 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2066 2534 2888 3083 10
Size: 54 1425 343 234 468 354 195 3066
Fragments arranged by size:
3066 1425 468 354 343 234 195 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2255 4097 4456 4615 518
Size: 648 1089 1842 359 159 2042
Fragments arranged by size:
2042 1842 1089 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2058 2085 2530 2857 2863 2945 3207 3284
Size: 452 27 445 327 6 82 262 77
Cuts at: 3284 3555 4232 5380 5625 1606
Size: 271 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 452 445 327 271 262
245 82 77 27 6
NaeI GCC'GGC
Cuts at: 2126 2694 3134 2126
Size: 568 440 5131
Fragments arranged by size:
5131 568 440
NarI GG'CG_CC
Cuts at: 3226 3226
Size: 6139
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5587
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6139
NgoMIV G'CCGG_C
Cuts at: 2124 2692 3132 2124
Size: 568 440 5131
Fragments arranged by size:
5131 568 440
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6139
NotI GC'GGCC_GC
Cuts at: 3586 3586
Size: 6139
NspI r_CATG'y
Cuts at: 1132 1229 2326 3497 5042 1132
Size: 97 1097 1171 1545 2229
Fragments arranged by size:
2229 1545 1171 1097 97
PasI CC'CwG_GG
Cuts at: 1348 2221 3238 3373 1348
Size: 873 1017 135 4114
Fragments arranged by size:
4114 1017 873 135
PciI A'CATG_T
Cuts at: 2322 5038 2322
Size: 2716 3423
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2088 1108
Size: 980 5159
PfoI T'CCnGG_A
Cuts at: 2790 2790
Size: 6139
PmeI GTTT'AAAC
Cuts at: 3443 3443
Size: 6139
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4208 4240 5721 5753 4208
Size: 32 1481 32 4594
Fragments arranged by size:
4594 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2176 3427 2176
Size: 1251 4888
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6139
PsiI TTA'TAA
Cuts at: 3709 3709
Size: 6139
PstI C_TGCA'G
Cuts at: 830 2294 2871 3491 830
Size: 1464 577 620 3478
Fragments arranged by size:
3478 1464 620 577
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6139
PvuII CAG'CTG
Cuts at: 2058 2085 3207 3284 2058
Size: 27 1122 77 4913
Fragments arranged by size:
4913 1122 77 27
SacI G_AGCT'C
Cuts at: 2458 3002 2458
Size: 544 5595
SalI G'TCGA_C
Cuts at: 1936 3481 1936
Size: 1545 4594
SanDI GG'GwC_CC
Cuts at: 2176 2176
Size: 6139
SbfI CC_TGCA'GG
Cuts at: 3491 3491
Size: 6139
ScaI AGT'ACT
Cuts at: 4387 6099 4387
Size: 1712 4427
SexAI A'CCwGG_T
Cuts at: 2511 2511
Size: 6139
SfcI C'TryA_G
Cuts at: 826 1045 2290 2867 3487 4622 5303 5494 826
Size: 219 1245 577 620 1135 681 191 1471
Fragments arranged by size:
1471 1245 1135 681 620 577 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2957 2957
Size: 6139
SfoI GGC'GCC
Cuts at: 3227 3227
Size: 6139
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6139
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6139
SmaI CCC'GGG
Cuts at: 1872 3466 1872
Size: 1594 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4243 5144 5406 820
Size: 197 926 2300 901 262 1553
Fragments arranged by size:
2300 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3930 493
Size: 3437 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6139
SphI G_CATG'C
Cuts at: 1132 3497 1132
Size: 2365 3774
SspI AAT'ATT
Cuts at: 5 52 4063 5
Size: 47 4011 2081
Fragments arranged by size:
4011 2081 47
StuI AGG'CCT
Cuts at: 1517 3026 1517
Size: 1509 4630
StyI C'CwwG_G
Cuts at: 513 1065 3021 3354 3405 3578 513
Size: 552 1956 333 51 173 3074
Fragments arranged by size:
3074 1956 552 333 173 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2095 2384 3077 3110 3182 4166 4183
Size: 1255 515 289 693 33 72 984 17
Cuts at: 4183 4336 4521 4966 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 984 693 515 445 289 185
153 72 33 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3072 4385 6097
Size: 274 80 33 51 1443 1095 1313 1712
Cuts at: 6097 96
Size: 138
Fragments arranged by size:
1712 1443 1313 1095 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6099 6131 6099
Size: 32 6107
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6139
XbaI T'CTAG_A
Cuts at: 3475 3475
Size: 6139
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2085 2542 3076 3308 2085
Size: 457 534 232 4916
Fragments arranged by size:
4916 534 457 232
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6139
XmaI C'CCGG_G
Cuts at: 1870 3464 1870
Size: 1594 4545
XmnI GAAnn'nnTTC
Cuts at: 4268 4268
Size: 6139
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3947 276
Size: 53 83 186 936 2413 2468
Fragments arranged by size:
2468 2413 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI
FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacII SapI SrfI SwaI