Restriction map for
pFN21AE9445
(Circular) (Six-base) MAPSORT of: pFN21AE9445.seq Check: 8340 from: 1 to: 5734
pFN21AE9445 5734 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2537 2537
Size: 5734
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3544 278
Size: 53 83 186 936 2008 2468
Fragments arranged by size:
2468 2008 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2874 3077 1937
Size: 937 203 4594
Fragments arranged by size:
4594 937 203
Acc65I G'GTAC_C
Cuts at: 3055 3055
Size: 5734
AclI AA'CG_TT
Cuts at: 1563 3861 4234 1563
Size: 2298 373 3063
Fragments arranged by size:
3063 2298 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2269 3797 5181 5449 1632
Size: 637 1528 1384 268 1917
Fragments arranged by size:
1917 1528 1384 637 268
AfeI AGC'GCT
Cuts at: 2426 2702 2426
Size: 276 5458
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5537
AflIII A'CryG_T
Cuts at: 1203 4633 5610 1203
Size: 3430 977 1327
Fragments arranged by size:
3430 1327 977
AgeI A'CCGG_T
Cuts at: 4565 4565
Size: 5734
AhdI GACnn_n'nnGTC
Cuts at: 4463 4463
Size: 5734
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5734
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5734
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2222 2254 2222
Size: 32 5702
AlwNI CAG_nnn'CTG
Cuts at: 2908 3477 5049 2908
Size: 569 1572 3593
Fragments arranged by size:
3593 1572 569
ApaI G_GGCC'C
Cuts at: 2572 2689 2944 2572
Size: 117 255 5362
Fragments arranged by size:
5362 255 117
ApaLI G'TGCA_C
Cuts at: 3791 4947 3791
Size: 1156 4578
ApoI r'AATT_y
Cuts at: 1493 3043 3269 3421 5530 1493
Size: 1550 226 152 2109 1697
Fragments arranged by size:
2109 1697 1550 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4288 160
Size: 872 3256 1606
Fragments arranged by size:
3256 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5734
AvaI C'yCGr_G
Cuts at: 1870 1943 2819 3019 3059 1870
Size: 73 876 200 40 4545
Fragments arranged by size:
4545 876 200 73 40
BamHI G'GATC_C
Cuts at: 3064 3431 3064
Size: 367 5367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2460 2694 3055 4510 618
Size: 325 217 627 673 234 361 1455 1842
Fragments arranged by size:
1842 1455 673 627 361 325 234 217
BanII G_rGCy'C
Cuts at: 1391 2376 2572 2689 2944 3053 1391
Size: 985 196 117 255 109 4072
Fragments arranged by size:
4072 985 255 196 117 109
BbeI G_GCGC'C
Cuts at: 2464 2698 2464
Size: 234 5500
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2737 2788 2923 928
Size: 971 838 51 135 3739
Fragments arranged by size:
3739 971 838 135 51
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3925 3959 1903
Size: 34 1988 34 3678
Fragments arranged by size:
3678 1988 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3626 4836 3626
Size: 1210 4524
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5734
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2175 2184 4345
Size: 107 122 71 631 605 503 9 2161
Cuts at: 4345 136
Size: 1525
Fragments arranged by size:
2161 1525 631 605 503 122 107 71
9
BglII A'GATC_T
Cuts at: 5729 5729
Size: 5734
BlpI GC'TnA_GC
Cuts at: 2958 3151 2958
Size: 193 5541
Bme1580I G_kGCm'C
Cuts at: 1790 2060 2333 2572 2689 2944 3795 4951 1790
Size: 270 273 239 117 255 851 1156 2573
Fragments arranged by size:
2573 1156 851 273 270 255 239 117
BmgBI CAC'GTC
Cuts at: 1318 2722 1318
Size: 1404 4330
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3465 4423 442
Size: 416 53 83 399 24 2048 958 1753
Fragments arranged by size:
2048 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5734
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4394 1239
Size: 3155 2579
Bpu10I CC'TnA_GC
Cuts at: 2066 2921 2066
Size: 855 4879
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3859 4724 5022 3859
Size: 865 298 4571
Fragments arranged by size:
4571 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2743 4397 882
Size: 617 1244 1654 2219
Fragments arranged by size:
2219 1654 1244 617
BsaAI yAC'GTr
Cuts at: 493 1204 2674 3525 493
Size: 711 1470 851 2702
Fragments arranged by size:
2702 1470 851 711
BsaBI GATnn'nnATC
Cuts at: 5728 5728
Size: 5734
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2461 2695 3541 3923
Size: 53 83 186 936 928 234 846 382
Cuts at: 3923 4558 275
Size: 635 1451
Fragments arranged by size:
1451 936 928 846 635 382 234 186
83 53
BsaWI w'CCGG_w
Cuts at: 4167 4565 4839 4986 5397 4167
Size: 398 274 147 411 4504
Fragments arranged by size:
4504 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2222 2252 2996 3026 1178
Size: 30 165 30 819 30 744 30 3886
Fragments arranged by size:
3886 819 744 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2153 1186
Size: 967 4767
BseYI C'CCAG_C
Cuts at: 2962 4937 5433 2962
Size: 1975 496 3263
Fragments arranged by size:
3263 1975 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2237 2519 2654 1163
Size: 573 501 282 135 4243
Fragments arranged by size:
4243 573 501 282 135
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2139 2412 2487 3184 3945 4973
Size: 275 186 138 273 75 697 761 1028
Cuts at: 4973 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 697 275 273 186 138
75
BsiHKAI G_wGCw'C
Cuts at: 3795 3880 4951 3795
Size: 85 1071 4578
Fragments arranged by size:
4578 1071 85
BsmI GAATG_Cn'
Cuts at: 3245 3338 3245
Size: 93 5641
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2060 2333 2376 2572 2689 2944 3053
Size: 399 270 273 43 196 117 255 109
Cuts at: 3053 3795 3880 4951 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 273 270 255 196
117 109 85 43
BspEI T'CCGG_A
Cuts at: 5397 5397
Size: 5734
BspHI T'CATG_A
Cuts at: 2142 3623 5353 2142
Size: 1481 1730 2523
Fragments arranged by size:
2523 1730 1481
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2537 3089 844
Size: 1693 552 3489
Fragments arranged by size:
3489 1693 552
BsrBI CCG'CTC
Cuts at: 1394 1823 2638 2920 3621 1394
Size: 429 815 282 701 3507
Fragments arranged by size:
3507 815 701 429 282
BsrDI GCAATG_nn'
Cuts at: 65 1248 4229 4403 65
Size: 1183 2981 174 1396
Fragments arranged by size:
2981 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2390 2407 2463 4378 4565 1700
Size: 110 580 17 56 1915 187 2869
Fragments arranged by size:
2869 1915 580 187 110 56 17
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5734
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5734
BssSI C'ACGA_G
Cuts at: 3794 4806 3794
Size: 1012 4722
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2358 794
Size: 1564 4170
BstBI TT'CG_AA
Cuts at: 4538 4538
Size: 5734
BstEII G'GTnAC_C
Cuts at: 1184 2524 1184
Size: 1340 4394
BstYI r'GATC_y
Cuts at: 1972 2802 3064 3431 3816 3833 5285 5371 5383
Size: 830 262 367 385 17 1452 86 12
Cuts at: 5383 5729 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 830 385 367 346 262 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2487 2563 2733 3000 513
Size: 552 939 483 76 170 267 3247
Fragments arranged by size:
3247 939 552 483 267 170 76
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2135 487
Size: 683 56 909 4086
Fragments arranged by size:
4086 909 683 56
BtsI GCAGTG_nn'
Cuts at: 3017 3250 4044 4064 3017
Size: 233 794 20 4687
Fragments arranged by size:
4687 794 233 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5734
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5699
DraI TTT'AAA
Cuts at: 3038 3038
Size: 5734
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4741 809
Size: 507 3425 1802
Fragments arranged by size:
3425 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2136 2259 2409
Size: 54 1425 50 275 18 306 123 150
Cuts at: 2409 2484 2730 2883 3181 4070 5699 8
Size: 75 246 153 298 889 1629 43
Fragments arranged by size:
1629 1425 889 306 298 275 246 153
150 123 75 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2136 2409 2484 3181 1537
Size: 275 324 273 75 697 4090
Fragments arranged by size:
4090 697 324 275 273 75
EarI CTCTTCn'nnn_
Cuts at: 1353 2288 2792 3664 1353
Size: 935 504 872 3423
Fragments arranged by size:
3423 935 872 504
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4321 4695 4841 387
Size: 1064 138 2732 374 146 1280
Fragments arranged by size:
2732 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2269 3797 4394 5181 5449 1239
Size: 393 637 1528 597 787 268 1524
Fragments arranged by size:
1528 1524 787 637 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2069 2069
Size: 5734
EcoO109I rG'GnC_Cy
Cuts at: 2074 2365 2509 2568 2569 2941 2074
Size: 291 144 59 1 372 4867
Fragments arranged by size:
4867 372 291 144 59 1
EcoRI G'AATT_C
Cuts at: 3043 3421 3043
Size: 378 5356
FspI TGC'GCA
Cuts at: 4240 4240
Size: 5734
HaeII r_GCGC'y
Cuts at: 1678 2268 2421 2428 2464 2479 2698 2704 2862
Size: 590 153 7 36 15 219 6 158
Cuts at: 2862 2958 4881 1678
Size: 96 1923 2531
Fragments arranged by size:
2531 1923 590 219 158 153 96 36
15 7 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2998 3030 4375 4407 4449
Size: 32 135 32 1426 32 1345 32 42
Cuts at: 4449 4481 1373
Size: 32 2626
Fragments arranged by size:
2626 1426 1345 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3078 3324 669
Size: 988 281 1140 246 3079
Fragments arranged by size:
3079 1140 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5734
HpaI GTT'AAC
Cuts at: 3324 3324
Size: 5734
KasI G'GCGC_C
Cuts at: 2460 2694 2460
Size: 234 5500
KpnI G_GTAC'C
Cuts at: 3059 3059
Size: 5734
MfeI C'AATT_G
Cuts at: 3333 3333
Size: 5734
MluI A'CGCG_T
Cuts at: 5610 5610
Size: 5734
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4848 5032 1305
Size: 3543 184 2007
Fragments arranged by size:
3543 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2261 2732 10
Size: 54 1425 343 429 471 3012
Fragments arranged by size:
3012 1425 471 429 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3005 3692 4051 4210 518
Size: 648 1839 687 359 159 2042
Fragments arranged by size:
2042 1839 687 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2565 2905 2938 3150 3827 4975 5220 1606
Size: 959 340 33 212 677 1148 245 2120
Fragments arranged by size:
2120 1148 959 677 340 245 212 33
NaeI GCC'GGC
Cuts at: 2409 2465 2409
Size: 56 5678
NarI GG'CG_CC
Cuts at: 2461 2695 2461
Size: 234 5500
NcoI C'CATG_G
Cuts at: 513 1065 2004 2733 3000 513
Size: 552 939 729 267 3247
Fragments arranged by size:
3247 939 729 552 267
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5734
NgoMIV G'CCGG_C
Cuts at: 2407 2463 2407
Size: 56 5678
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5734
NotI GC'GGCC_GC
Cuts at: 3181 3181
Size: 5734
NruI TCG'CGA
Cuts at: 2120 2120
Size: 5734
NspI r_CATG'y
Cuts at: 1132 1229 3092 4637 1132
Size: 97 1863 1545 2229
Fragments arranged by size:
2229 1863 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5734
PciI A'CATG_T
Cuts at: 4633 4633
Size: 5734
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5734
PmeI GTTT'AAAC
Cuts at: 3038 3038
Size: 5734
PmlI CAC'GTG
Cuts at: 2674 2674
Size: 5734
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2222 2254 3803 3835 5316 5348 2222
Size: 32 1549 32 1481 32 2608
Fragments arranged by size:
2608 1549 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2365 2509 2365
Size: 144 5590
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5734
PsiI TTA'TAA
Cuts at: 3304 3304
Size: 5734
PspOMI G'GGCC_C
Cuts at: 2568 2685 2940 2568
Size: 117 255 5362
Fragments arranged by size:
5362 255 117
PstI C_TGCA'G
Cuts at: 830 2588 3086 830
Size: 1758 498 3478
Fragments arranged by size:
3478 1758 498
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5734
SacII CC_GC'GG
Cuts at: 2566 2566
Size: 5734
SalI G'TCGA_C
Cuts at: 1936 3076 1936
Size: 1140 4594
SbfI CC_TGCA'GG
Cuts at: 2588 3086 2588
Size: 498 5236
ScaI AGT'ACT
Cuts at: 3982 5694 3982
Size: 1712 4022
SfcI C'TryA_G
Cuts at: 826 1045 2086 2584 3082 4217 4898 5089 826
Size: 219 1041 498 498 1135 681 191 1471
Fragments arranged by size:
1471 1135 1041 681 498 498 219 191
SfoI GGC'GCC
Cuts at: 2462 2696 2462
Size: 234 5500
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5734
SgrAI Cr'CCGG_yG
Cuts at: 1700 2463 1700
Size: 763 4971
SmaI CCC'GGG
Cuts at: 1872 3021 3061 1872
Size: 1149 40 4545
Fragments arranged by size:
4545 1149 40
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3838 4739 5001 820
Size: 197 926 1895 901 262 1553
Fragments arranged by size:
1895 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3525 493
Size: 3032 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5734
SphI G_CATG'C
Cuts at: 1132 3092 1132
Size: 1960 3774
SspI AAT'ATT
Cuts at: 5 52 3658 5
Size: 47 3606 2081
Fragments arranged by size:
3606 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5734
StyI C'CwwG_G
Cuts at: 513 1065 2004 2733 3000 3173 513
Size: 552 939 729 267 173 3074
Fragments arranged by size:
3074 939 729 552 267 173
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2425 3761 3778 3931 4116 4561 325
Size: 1255 845 1336 17 153 185 445 1498
Fragments arranged by size:
1498 1336 1255 845 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3980 5692 96
Size: 274 80 33 51 1443 2003 1712 138
Fragments arranged by size:
2003 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2996 3028 5694 5726 2996
Size: 32 2666 32 3004
Fragments arranged by size:
3004 2666 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5734
XbaI T'CTAG_A
Cuts at: 3070 3070
Size: 5734
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2269 2269
Size: 5734
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5734
XmaI C'CCGG_G
Cuts at: 1870 3019 3059 1870
Size: 1149 40 4545
Fragments arranged by size:
4545 1149 40
XmnI GAAnn'nnTTC
Cuts at: 2234 3863 2234
Size: 1629 4105
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3542 276
Size: 53 83 186 936 2008 2468
Fragments arranged by size:
2468 2008 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SacII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI
FspAI NsiI PacI PfoI PspXI PsrI PvuII RsrII
SacI SanDI SapI SexAI SfiI SrfI SwaI