Restriction map for
pFN21AE9443
(Circular) (Six-base) MAPSORT of: pFN21AE9443.seq Check: 1366 from: 1 to: 9082
pFN21AE9443 9082 bp
With 182 enzymes: SgfI *
December 8, 2014 13:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6892 278
Size: 53 83 186 936 5356 2468
Fragments arranged by size:
5356 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2919 4299 4412 6425 1937
Size: 982 1380 113 2013 4594
Fragments arranged by size:
4594 2013 1380 982 113
Acc65I G'GTAC_C
Cuts at: 6403 6403
Size: 9082
AclI AA'CG_TT
Cuts at: 1563 7209 7582 1563
Size: 5646 373 3063
Fragments arranged by size:
5646 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2480 3216 3672 3882 5284 5589 6102 6245
Size: 848 736 456 210 1402 305 513 143
Cuts at: 6245 7145 8529 8797 1632
Size: 900 1384 268 1917
Fragments arranged by size:
1917 1402 1384 900 848 736 513 456
305 268 210 143
AfeI AGC'GCT
Cuts at: 2188 2188
Size: 9082
AflII C'TTAA_G
Cuts at: 820 1017 3821 820
Size: 197 2804 6081
Fragments arranged by size:
6081 2804 197
AflIII A'CryG_T
Cuts at: 1203 3138 4457 7981 8958 1203
Size: 1935 1319 3524 977 1327
Fragments arranged by size:
3524 1935 1327 1319 977
AgeI A'CCGG_T
Cuts at: 7913 7913
Size: 9082
AhdI GACnn_n'nnGTC
Cuts at: 7811 7811
Size: 9082
AleI CACnn'nnGTG
Cuts at: 1166 3215 4559 5718 1166
Size: 2049 1344 1159 4530
Fragments arranged by size:
4530 2049 1344 1159
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9082
AlwNI CAG_nnn'CTG
Cuts at: 3742 5612 6080 6825 8397 3742
Size: 1870 468 745 1572 4427
Fragments arranged by size:
4427 1870 1572 745 468
ApaI G_GGCC'C
Cuts at: 3551 3551
Size: 9082
ApaLI G'TGCA_C
Cuts at: 5807 7139 8295 5807
Size: 1332 1156 6594
Fragments arranged by size:
6594 1332 1156
ApoI r'AATT_y
Cuts at: 1493 2511 2696 3366 3889 4058 4685 6391 6617
Size: 1018 185 670 523 169 627 1706 226
Cuts at: 6617 6769 8878 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1706 1697 1018 670 627 523 226
185 169 152
AseI AT'TA_AT
Cuts at: 160 1032 5178 7636 160
Size: 872 4146 2458 1606
Fragments arranged by size:
4146 2458 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9082
AvaI C'yCGr_G
Cuts at: 1870 1943 2158 5846 5972 6407 1870
Size: 73 215 3688 126 435 4545
Fragments arranged by size:
4545 3688 435 215 126 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4648 4681 4648
Size: 33 9049
BamHI G'GATC_C
Cuts at: 6412 6779 6412
Size: 367 8715
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5977 6403 7858 618
Size: 325 217 627 4190 426 1455 1842
Fragments arranged by size:
4190 1842 1455 627 426 325 217
BanII G_rGCy'C
Cuts at: 1391 3551 5853 5958 6401 1391
Size: 2160 2302 105 443 4072
Fragments arranged by size:
4072 2302 2160 443 105
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2004 2653 3809 5132 6186 928
Size: 971 105 649 1156 1323 1054 3824
Fragments arranged by size:
3824 1323 1156 1054 971 649 105
BbvCI CC'TCA_GC
Cuts at: 5991 5991
Size: 9082
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2912 2946 7273 7307 1903
Size: 34 975 34 4327 34 3678
Fragments arranged by size:
4327 3678 975 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5647 5662 6974 8184 5647
Size: 15 1312 1210 6545
Fragments arranged by size:
6545 1312 1210 15
BclI T'GATC_A
Cuts at: 1548 5034 5790 1548
Size: 3486 756 4840
Fragments arranged by size:
4840 3486 756
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7693 136
Size: 107 122 71 631 605 6021 1525
Fragments arranged by size:
6021 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2146 9077 2146
Size: 6931 2151
BlpI GC'TnA_GC
Cuts at: 3237 6499 3237
Size: 3262 5820
Bme1580I G_kGCm'C
Cuts at: 1790 3551 5811 7143 8299 1790
Size: 1761 2260 1332 1156 2573
Fragments arranged by size:
2573 2260 1761 1332 1156
BmgBI CAC'GTC
Cuts at: 1318 4261 1318
Size: 2943 6139
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2334 3586 4220
Size: 416 53 83 399 24 917 1252 634
Cuts at: 4220 6813 7771 442
Size: 2593 958 1753
Fragments arranged by size:
2593 1753 1252 958 917 634 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9082
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3194 3330 5972 6178 7742 1239
Size: 1955 136 2642 206 1564 2579
Fragments arranged by size:
2642 2579 1955 1564 206 136
Bpu10I CC'TnA_GC
Cuts at: 5991 5991
Size: 9082
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2725 7207 8072 8370 2725
Size: 4482 865 298 3437
Fragments arranged by size:
4482 3437 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3850 3991 7745 882
Size: 617 2351 141 3754 2219
Fragments arranged by size:
3754 2351 2219 617 141
BsaAI yAC'GTr
Cuts at: 493 1204 3139 6873 493
Size: 711 1935 3734 2702
Fragments arranged by size:
3734 2702 1935 711
BsaBI GATnn'nnATC
Cuts at: 3114 9076 3114
Size: 5962 3120
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6889 7271 7906 275
Size: 53 83 186 936 5356 382 635 1451
Fragments arranged by size:
5356 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4164 6154 7515 7913 8187 8334 8745 4164
Size: 1990 1361 398 274 147 411 4501
Fragments arranged by size:
4501 1990 1361 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3048 3078 3936 3966 1178
Size: 30 165 30 1645 30 858 30 6294
Fragments arranged by size:
6294 1645 858 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3069 5198 6214 1186
Size: 1883 2129 1016 4054
Fragments arranged by size:
4054 2129 1883 1016
BseYI C'CCAG_C
Cuts at: 3671 8285 8781 3671
Size: 4614 496 3972
Fragments arranged by size:
4614 3972 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4823 5696 1163
Size: 573 3087 873 4549
Fragments arranged by size:
4549 3087 873 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6532 7293 8321 1540
Size: 275 186 4531 761 1028 2301
Fragments arranged by size:
4531 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3299 5263 5383 5811 5853 5958 7143 7228 8299
Size: 1964 120 428 42 105 1185 85 1071
Cuts at: 8299 3299
Size: 4082
Fragments arranged by size:
4082 1964 1185 1071 428 120 105 85
42
BsmI GAATG_Cn'
Cuts at: 2031 5059 6593 6686 2031
Size: 3028 1534 93 4427
Fragments arranged by size:
4427 3028 1534 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3299 3551 5263 5383 5811 5853 5958
Size: 399 1509 252 1712 120 428 42 105
Cuts at: 5958 6401 7143 7228 8299 1391
Size: 443 742 85 1071 2174
Fragments arranged by size:
2174 1712 1509 1071 742 443 428 399
252 120 105 85 42
BspEI T'CCGG_A
Cuts at: 6154 8745 6154
Size: 2591 6491
BspHI T'CATG_A
Cuts at: 2175 4531 6971 8701 2175
Size: 2356 2440 1730 2556
Fragments arranged by size:
2556 2440 2356 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5079 6437 844
Size: 4235 1358 3489
Fragments arranged by size:
4235 3489 1358
BsrBI CCG'CTC
Cuts at: 1394 1823 6969 1394
Size: 429 5146 3507
Fragments arranged by size:
5146 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4242 4677 5570 7577 7751 65
Size: 1183 2994 435 893 2007 174 1396
Fragments arranged by size:
2994 2007 1396 1183 893 435 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7726 7913 1700
Size: 110 5916 187 2869
Fragments arranged by size:
5916 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9082
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9082
BssSI C'ACGA_G
Cuts at: 7142 8154 7142
Size: 1012 8070
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2942 3911 4778 794
Size: 2148 969 867 5098
Fragments arranged by size:
5098 2148 969 867
BstBI TT'CG_AA
Cuts at: 7886 7886
Size: 9082
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9082
BstXI CCAn_nnnn'nTGG
Cuts at: 2775 3640 4210 2775
Size: 865 570 7647
Fragments arranged by size:
7647 865 570
BstYI r'GATC_y
Cuts at: 1972 2146 4044 6412 6779 7164 7181 8633 8719
Size: 174 1898 2368 367 385 17 1452 86
Cuts at: 8719 8731 9077 1972
Size: 12 346 1977
Fragments arranged by size:
2368 1977 1898 1452 385 367 346 174
86 17 12
BstZ17I GTA'TAC
Cuts at: 2920 4300 2920
Size: 1380 7702
Bsu36I CC'TnA_GG
Cuts at: 3483 3543 3483
Size: 60 9022
BtgI C'CryG_G
Cuts at: 513 1065 2004 4621 5545 5664 513
Size: 552 939 2617 924 119 3931
Fragments arranged by size:
3931 2617 939 924 552 119
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2167 487
Size: 683 56 941 7402
Fragments arranged by size:
7402 941 683 56
BtsI GCAGTG_nn'
Cuts at: 2170 3111 4254 5896 6598 7392 7412 2170
Size: 941 1143 1642 702 794 20 3840
Fragments arranged by size:
3840 1642 1143 941 794 702 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9082
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4879 4914 529
Size: 35 4315 35 4697
Fragments arranged by size:
4697 4315 35 35
DraI TTT'AAA
Cuts at: 4057 6386 4057
Size: 2329 6753
DraIII CAC_nnn'GTG
Cuts at: 3580 5043 3580
Size: 1463 7619
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6056 8089 809
Size: 507 4740 2033 1802
Fragments arranged by size:
4740 2033 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 6250 6529 7418
Size: 54 1425 50 275 18 4420 279 889
Cuts at: 7418 9047 8
Size: 1629 43
Fragments arranged by size:
4420 1629 1425 889 279 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6529 1537
Size: 275 4717 4090
Fragments arranged by size:
4717 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2499 2895 5136 5213 5538 7012 1353
Size: 1146 396 2241 77 325 1474 3423
Fragments arranged by size:
3423 2241 1474 1146 396 325 77
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7669 8043 8189 387
Size: 1064 138 6080 374 146 1280
Fragments arranged by size:
6080 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5851 5956 5851
Size: 105 8977
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2480 3194 3216 3330 3672 3882 5284
Size: 393 848 714 22 114 342 210 1402
Cuts at: 5284 5589 5972 6102 6178 6245 7145 7742 8529
Size: 305 383 130 76 67 900 597 787
Cuts at: 8529 8797 1239
Size: 268 1524
Fragments arranged by size:
1524 1402 900 848 787 714 597 393
383 342 305 268 210 130 114 76
67 22
EcoNI CCTnn'n_nnAGG
Cuts at: 6196 6196
Size: 9082
EcoO109I rG'GnC_Cy
Cuts at: 2418 2488 3287 3547 3548 2418
Size: 70 799 260 1 7952
Fragments arranged by size:
7952 799 260 70 1
EcoRI G'AATT_C
Cuts at: 3889 6391 6769 3889
Size: 2502 378 6202
Fragments arranged by size:
6202 2502 378
EcoRV GAT'ATC
Cuts at: 2794 2794
Size: 9082
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3291 3291
Size: 9082
FspI TGC'GCA
Cuts at: 7588 7588
Size: 9082
HaeII r_GCGC'y
Cuts at: 1678 2190 5124 8229 1678
Size: 512 2934 3105 2531
Fragments arranged by size:
3105 2934 2531 512
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2521 2553 3936 3968 5188
Size: 32 135 32 949 32 1383 32 1220
Cuts at: 5188 5220 5906 5938 7723 7755 7797 7829 1373
Size: 32 686 32 1785 32 42 32 2626
Fragments arranged by size:
2626 1785 1383 1220 949 686 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3835 4435 6426 6672 669
Size: 988 281 1897 600 1991 246 3079
Fragments arranged by size:
3079 1991 1897 988 600 281 246
HindIII A'AGCT_T
Cuts at: 748 3864 4944 748
Size: 3116 1080 4886
Fragments arranged by size:
4886 3116 1080
HpaI GTT'AAC
Cuts at: 6672 6672
Size: 9082
KpnI G_GTAC'C
Cuts at: 6407 6407
Size: 9082
MfeI C'AATT_G
Cuts at: 2430 3021 3828 6681 2430
Size: 591 807 2853 4831
Fragments arranged by size:
4831 2853 807 591
MluI A'CGCG_T
Cuts at: 8958 8958
Size: 9082
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2180 4120 4879 5073 8196 8380 1305
Size: 875 1940 759 194 3123 184 2007
Fragments arranged by size:
3123 2007 1940 875 759 194 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 6252 10
Size: 54 1425 343 4420 2840
Fragments arranged by size:
4420 2840 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2405 2773 3215 4247 4559 5718 7040
Size: 648 1239 368 442 1032 312 1159 1322
Cuts at: 7040 7399 7558 518
Size: 359 159 2042
Fragments arranged by size:
2042 1322 1239 1159 1032 648 442 368
359 312 159
MspA1I CmG'CkG
Cuts at: 1606 2875 3215 3236 3589 3733 3767 4910 5995
Size: 1269 340 21 353 144 34 1143 1085
Cuts at: 5995 6367 6498 7175 8323 8568 1606
Size: 372 131 677 1148 245 2120
Fragments arranged by size:
2120 1269 1148 1143 1085 677 372 353
340 245 144 131 34 21
NcoI C'CATG_G
Cuts at: 513 1065 2004 5664 513
Size: 552 939 3660 3931
Fragments arranged by size:
3931 3660 939 552
NdeI CA'TA_TG
Cuts at: 387 3885 387
Size: 3498 5584
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9082
NotI GC'GGCC_GC
Cuts at: 6529 6529
Size: 9082
NspI r_CATG'y
Cuts at: 1132 1229 3810 4467 4734 6130 6235 6440 7985
Size: 97 2581 657 267 1396 105 205 1545
Cuts at: 7985 1132
Size: 2229
Fragments arranged by size:
2581 2229 1545 1396 657 267 205 105
97
PasI CC'CwG_GG
Cuts at: 1348 4417 5945 1348
Size: 3069 1528 4485
Fragments arranged by size:
4485 3069 1528
PciI A'CATG_T
Cuts at: 7981 7981
Size: 9082
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2354 6242 1108
Size: 1246 3888 3948
Fragments arranged by size:
3948 3888 1246
PfoI T'CCnGG_A
Cuts at: 2353 4011 6194 2353
Size: 1658 2183 5241
Fragments arranged by size:
5241 2183 1658
PmeI GTTT'AAAC
Cuts at: 6386 6386
Size: 9082
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5365 5397 7151 7183 8664 8696 5365
Size: 32 1754 32 1481 32 5751
Fragments arranged by size:
5751 1754 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2418 2488 2418
Size: 70 9012
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9082
PsiI TTA'TAA
Cuts at: 3036 3780 6652 3036
Size: 744 2872 5466
Fragments arranged by size:
5466 2872 744
PspOMI G'GGCC_C
Cuts at: 3547 3547
Size: 9082
PspXI vC'TCGA_Gb
Cuts at: 5846 5972 5846
Size: 126 8956
PstI C_TGCA'G
Cuts at: 830 2506 3106 3738 6434 830
Size: 1676 600 632 2696 3478
Fragments arranged by size:
3478 2696 1676 632 600
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9082
PvuII CAG'CTG
Cuts at: 2875 3215 3236 3589 3733 3767 4910 6367 2875
Size: 340 21 353 144 34 1143 1457 5590
Fragments arranged by size:
5590 1457 1143 353 340 144 34 21
SacI G_AGCT'C
Cuts at: 5853 5958 5853
Size: 105 8977
SalI G'TCGA_C
Cuts at: 1936 6424 1936
Size: 4488 4594
SapI GCTCTTCn'nnn_
Cuts at: 5136 5136
Size: 9082
SbfI CC_TGCA'GG
Cuts at: 6434 6434
Size: 9082
ScaI AGT'ACT
Cuts at: 7330 9042 7330
Size: 1712 7370
SexAI A'CCwGG_T
Cuts at: 5153 5153
Size: 9082
SfcI C'TryA_G
Cuts at: 826 1045 2142 2502 3102 3303 3734 4628 6092
Size: 219 1097 360 600 201 431 894 1464
Cuts at: 6092 6430 7565 8246 8437 826
Size: 338 1135 681 191 1471
Fragments arranged by size:
1471 1464 1135 1097 894 681 600 431
360 338 219 201 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9082
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9082
SmaI CCC'GGG
Cuts at: 1872 2160 6409 1872
Size: 288 4249 4545
Fragments arranged by size:
4545 4249 288
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2704 3821 5846 5972 7186 8087
Size: 197 926 761 1117 2025 126 1214 901
Cuts at: 8087 8349 820
Size: 262 1553
Fragments arranged by size:
2025 1553 1214 1117 926 901 761 262
197 126
SnaBI TAC'GTA
Cuts at: 493 6873 493
Size: 6380 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9082
SphI G_CATG'C
Cuts at: 1132 4467 4734 6130 6235 6440 1132
Size: 3335 267 1396 105 205 3774
Fragments arranged by size:
3774 3335 1396 267 205 105
SrfI GCCC'GGGC
Cuts at: 2160 2160
Size: 9082
SspI AAT'ATT
Cuts at: 5 52 2689 3385 4006 4740 4844 4859 5020
Size: 47 2637 696 621 734 104 15 161
Cuts at: 5020 7006 5
Size: 1986 2081
Fragments arranged by size:
2637 2081 1986 734 696 621 161 104
47 15
StuI AGG'CCT
Cuts at: 1517 5325 1517
Size: 3808 5274
StyI C'CwwG_G
Cuts at: 513 1065 2004 3426 5664 6521 513
Size: 552 939 1422 2238 857 3074
Fragments arranged by size:
3074 2238 1422 939 857 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3568 4202 4549 4653 5101 7109 7126
Size: 1255 1988 634 347 104 448 2008 17
Cuts at: 7126 7279 7464 7909 325
Size: 153 185 445 1498
Fragments arranged by size:
2008 1988 1498 1255 634 448 445 347
185 153 104 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4031 4187 7328
Size: 274 80 33 51 1443 2054 156 3141
Cuts at: 7328 9040 96
Size: 1712 138
Fragments arranged by size:
3141 2054 1712 1443 274 156 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2628 2660 6080 6112 9042 9074 2628
Size: 32 3420 32 2930 32 2636
Fragments arranged by size:
3420 2930 2636 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5760 1316
Size: 4444 4638
XbaI T'CTAG_A
Cuts at: 6418 6418
Size: 9082
XhoI C'TCGA_G
Cuts at: 1943 5846 5972 1943
Size: 3903 126 5053
Fragments arranged by size:
5053 3903 126
XmaI C'CCGG_G
Cuts at: 1870 2158 6407 1870
Size: 288 4249 4545
Fragments arranged by size:
4545 4249 288
XmnI GAAnn'nnTTC
Cuts at: 4539 7211 4539
Size: 2672 6410
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6890 276
Size: 53 83 186 936 5356 2468
Fragments arranged by size:
5356 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BbeI BfrBI BplI BsiWI
BsmBI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PsrI RsrII SacII SanDI SfiI
SfoI SwaI XcmI