Restriction map for
pFN21AE9440
(Circular) (Six-base) MAPSORT of: pFN21AE9440.seq Check: 5662 from: 1 to: 5740
pFN21AE9440 5740 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3550 278
Size: 53 83 186 936 2014 2468
Fragments arranged by size:
2468 2014 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2812 3083 1937
Size: 875 271 4594
Fragments arranged by size:
4594 875 271
Acc65I G'GTAC_C
Cuts at: 3061 3061
Size: 5740
AclI AA'CG_TT
Cuts at: 1563 3867 4240 1563
Size: 2304 373 3063
Fragments arranged by size:
3063 2304 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3803 5187 5455 1632
Size: 2171 1384 268 1917
Fragments arranged by size:
2171 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5543
AflIII A'CryG_T
Cuts at: 1203 4639 5616 1203
Size: 3436 977 1327
Fragments arranged by size:
3436 1327 977
AgeI A'CCGG_T
Cuts at: 4571 4571
Size: 5740
AhdI GACnn_n'nnGTC
Cuts at: 4469 4469
Size: 5740
AleI CACnn'nnGTG
Cuts at: 1166 2986 1166
Size: 1820 3920
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2435 1184
Size: 1251 4489
AlwNI CAG_nnn'CTG
Cuts at: 3483 5055 3483
Size: 1572 4168
ApaLI G'TGCA_C
Cuts at: 3797 4953 3797
Size: 1156 4584
ApoI r'AATT_y
Cuts at: 1493 3049 3275 3427 5536 1493
Size: 1556 226 152 2109 1697
Fragments arranged by size:
2109 1697 1556 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4294 160
Size: 872 3262 1606
Fragments arranged by size:
3262 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5740
AvaI C'yCGr_G
Cuts at: 1870 1943 2901 3065 1870
Size: 73 958 164 4545
Fragments arranged by size:
4545 958 164 73
BamHI G'GATC_C
Cuts at: 3070 3437 3070
Size: 367 5373
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3061 4516 618
Size: 325 217 627 1274 1455 1842
Fragments arranged by size:
1842 1455 1274 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2223 2256 2790 2892 2902 3059 1391
Size: 832 33 534 102 10 157 4072
Fragments arranged by size:
4072 832 534 157 102 33 10
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4769
BbvCI CC'TCA_GC
Cuts at: 2919 2919
Size: 5740
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3931 3965 1903
Size: 34 1994 34 3678
Fragments arranged by size:
3678 1994 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3632 4842 3632
Size: 1210 4530
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5740
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4351 136
Size: 107 122 71 631 605 2679 1525
Fragments arranged by size:
2679 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5735 5735
Size: 5740
BlpI GC'TnA_GC
Cuts at: 3157 3157
Size: 5740
Bme1580I G_kGCm'C
Cuts at: 1790 2825 3801 4957 1790
Size: 1035 976 1156 2573
Fragments arranged by size:
2573 1156 1035 976
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5740
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2704 3471 4429
Size: 416 53 83 399 24 1287 767 958
Cuts at: 4429 442
Size: 1753
Fragments arranged by size:
1753 1287 958 767 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5740
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2052 2102 2878 4400 1239
Size: 813 50 776 1522 2579
Fragments arranged by size:
2579 1522 813 776 50
Bpu10I CC'TnA_GC
Cuts at: 2784 2919 2784
Size: 135 5605
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3865 4730 5028 3865
Size: 865 298 4577
Fragments arranged by size:
4577 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4403 882
Size: 617 2904 2219
Fragments arranged by size:
2904 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3531 493
Size: 711 2327 2702
Fragments arranged by size:
2702 2327 711
BsaBI GATnn'nnATC
Cuts at: 5734 5734
Size: 5740
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2055 3547 3929 4564
Size: 53 83 186 936 522 1492 382 635
Cuts at: 4564 275
Size: 1451
Fragments arranged by size:
1492 1451 936 635 522 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4173 4571 4845 4992 5403 4173
Size: 398 274 147 411 4510
Fragments arranged by size:
4510 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3024 3054 1178
Size: 30 165 30 1621 30 3864
Fragments arranged by size:
3864 1621 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2186 2936 3048 1186
Size: 1000 750 112 3878
Fragments arranged by size:
3878 1000 750 112
BseYI C'CCAG_C
Cuts at: 2190 3018 4943 5439 2190
Size: 828 1925 496 2491
Fragments arranged by size:
2491 1925 828 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5167
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2727 3190 3951 4979 1540
Size: 275 186 726 463 761 1028 2301
Fragments arranged by size:
2301 1028 761 726 463 275 186
BsiHKAI G_wGCw'C
Cuts at: 2256 2637 2790 2892 3801 3886 4957 2256
Size: 381 153 102 909 85 1071 3039
Fragments arranged by size:
3039 1071 909 381 153 102 85
BsiWI C'GTAC_G
Cuts at: 2275 2275
Size: 5740
BsmI GAATG_Cn'
Cuts at: 2793 3251 3344 2793
Size: 458 93 5189
Fragments arranged by size:
5189 458 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2569 2569
Size: 5740
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2223 2256 2637 2790 2825 2892 2902
Size: 399 433 33 381 153 35 67 10
Cuts at: 2902 3059 3801 3886 4957 1391
Size: 157 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 433 399 381 157 153
85 67 35 33 10
BspEI T'CCGG_A
Cuts at: 5403 5403
Size: 5740
BspHI T'CATG_A
Cuts at: 2457 3629 5359 2457
Size: 1172 1730 2838
Fragments arranged by size:
2838 1730 1172
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3095 844
Size: 2251 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3627 1394
Size: 429 1804 3507
Fragments arranged by size:
3507 1804 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2397 2733 2842 4235 4409 65
Size: 1183 1149 336 109 1393 174 1396
Fragments arranged by size:
1396 1393 1183 1149 336 174 109
BsrFI r'CCGG_y
Cuts at: 1700 1810 4384 4571 1700
Size: 110 2574 187 2869
Fragments arranged by size:
2869 2574 187 110
BsrGI T'GTAC_A
Cuts at: 96 2007 2772 96
Size: 1911 765 3064
Fragments arranged by size:
3064 1911 765
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5740
BssSI C'ACGA_G
Cuts at: 3800 4812 3800
Size: 1012 4728
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2841 794
Size: 2047 3693
BstBI TT'CG_AA
Cuts at: 4544 4544
Size: 5740
BstEII G'GTnAC_C
Cuts at: 1184 2743 1184
Size: 1559 4181
BstYI r'GATC_y
Cuts at: 1972 2154 2664 3070 3437 3822 3839 5291 5377
Size: 182 510 406 367 385 17 1452 86
Cuts at: 5377 5389 5735 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 510 406 385 367 346 182
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2066 513
Size: 552 1001 4187
Fragments arranged by size:
4187 1001 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5001
Fragments arranged by size:
5001 683 56
BtsI GCAGTG_nn'
Cuts at: 3256 4050 4070 3256
Size: 794 20 4926
Fragments arranged by size:
4926 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5740
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5705
DraI TTT'AAA
Cuts at: 3044 3044
Size: 5740
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2723 2726 4747 809
Size: 507 1407 3 2021 1802
Fragments arranged by size:
2021 1802 1407 507 3
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2091 3187 4076
Size: 54 1425 50 275 18 261 1096 889
Cuts at: 4076 5705 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1096 889 275 261 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3187 1537
Size: 275 1375 4090
Fragments arranged by size:
4090 1375 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2144 2204 2228 2543 2642 3670 1353
Size: 791 60 24 315 99 1028 3423
Fragments arranged by size:
3423 1028 791 315 99 60 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4327 4701 4847 387
Size: 1064 138 2738 374 146 1280
Fragments arranged by size:
2738 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2254 2788 2890 2254
Size: 534 102 5104
Fragments arranged by size:
5104 534 102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2052 2102 2878 3803 4400 5187 5455
Size: 393 420 50 776 925 597 787 268
Cuts at: 5455 1239
Size: 1524
Fragments arranged by size:
1524 925 787 776 597 420 393 268
50
EcoNI CCTnn'n_nnAGG
Cuts at: 2829 2829
Size: 5740
EcoRI G'AATT_C
Cuts at: 3049 3427 3049
Size: 378 5362
FspI TGC'GCA
Cuts at: 4246 4246
Size: 5740
HaeII r_GCGC'y
Cuts at: 1678 4887 1678
Size: 3209 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2959 2991 4381 4413 4455
Size: 32 135 32 1387 32 1390 32 42
Cuts at: 4455 4487 1373
Size: 32 2626
Fragments arranged by size:
2626 1390 1387 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3084 3330 669
Size: 988 281 1146 246 3079
Fragments arranged by size:
3079 1146 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5740
HpaI GTT'AAC
Cuts at: 3330 3330
Size: 5740
KpnI G_GTAC'C
Cuts at: 3065 3065
Size: 5740
MfeI C'AATT_G
Cuts at: 3339 3339
Size: 5740
MluI A'CGCG_T
Cuts at: 5616 5616
Size: 5740
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4854 5038 1305
Size: 3549 184 2007
Fragments arranged by size:
3549 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2093 10
Size: 54 1425 343 261 3657
Fragments arranged by size:
3657 1425 343 261 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2654 2986 3698 4057 4216 518
Size: 648 1488 332 712 359 159 2042
Fragments arranged by size:
2042 1488 712 648 359 332 159
MspA1I CmG'CkG
Cuts at: 1606 3156 3833 4981 5226 1606
Size: 1550 677 1148 245 2120
Fragments arranged by size:
2120 1550 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2066 513
Size: 552 1001 4187
Fragments arranged by size:
4187 1001 552
NdeI CA'TA_TG
Cuts at: 387 2944 387
Size: 2557 3183
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5740
NotI GC'GGCC_GC
Cuts at: 3187 3187
Size: 5740
NspI r_CATG'y
Cuts at: 1132 1229 3098 4643 1132
Size: 97 1869 1545 2229
Fragments arranged by size:
2229 1869 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5740
PciI A'CATG_T
Cuts at: 4639 4639
Size: 5740
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2754 1108
Size: 1646 4094
PfoI T'CCnGG_A
Cuts at: 2378 2378
Size: 5740
PmeI GTTT'AAAC
Cuts at: 3044 3044
Size: 5740
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3809 3841 5322 5354 3809
Size: 32 1481 32 4195
Fragments arranged by size:
4195 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5740
PsiI TTA'TAA
Cuts at: 3310 3310
Size: 5740
PstI C_TGCA'G
Cuts at: 830 2168 3092 830
Size: 1338 924 3478
Fragments arranged by size:
3478 1338 924
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5740
SacI G_AGCT'C
Cuts at: 2256 2790 2892 2256
Size: 534 102 5104
Fragments arranged by size:
5104 534 102
SalI G'TCGA_C
Cuts at: 1936 3082 1936
Size: 1146 4594
SapI GCTCTTCn'nnn_
Cuts at: 2228 2642 2228
Size: 414 5326
SbfI CC_TGCA'GG
Cuts at: 3092 3092
Size: 5740
ScaI AGT'ACT
Cuts at: 3988 5700 3988
Size: 1712 4028
SfcI C'TryA_G
Cuts at: 826 1045 2164 2800 3088 4223 4904 5095 826
Size: 219 1119 636 288 1135 681 191 1471
Fragments arranged by size:
1471 1135 1119 681 636 288 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5740
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5740
SmaI CCC'GGG
Cuts at: 1872 3067 1872
Size: 1195 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3844 4745 5007 820
Size: 197 926 1901 901 262 1553
Fragments arranged by size:
1901 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3531 493
Size: 3038 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5740
SphI G_CATG'C
Cuts at: 1132 3098 1132
Size: 1966 3774
SspI AAT'ATT
Cuts at: 5 52 3664 5
Size: 47 3612 2081
Fragments arranged by size:
3612 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5740
StyI C'CwwG_G
Cuts at: 513 1065 2066 3179 513
Size: 552 1001 1113 3074
Fragments arranged by size:
3074 1113 1001 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2204 2644 3767 3784 3937 4122 4567
Size: 1255 624 440 1123 17 153 185 445
Cuts at: 4567 325
Size: 1498
Fragments arranged by size:
1498 1255 1123 624 445 440 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2007 2518 2772
Size: 274 80 33 51 1443 30 511 254
Cuts at: 2772 3986 5698 96
Size: 1214 1712 138
Fragments arranged by size:
1712 1443 1214 511 274 254 138 80
51 33 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5700 5732 5700
Size: 32 5708
Tth111I GACn'n_nGTC
Cuts at: 1316 2723 1316
Size: 1407 4333
XbaI T'CTAG_A
Cuts at: 3076 3076
Size: 5740
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5740
XmaI C'CCGG_G
Cuts at: 1870 3065 1870
Size: 1195 4545
XmnI GAAnn'nnTTC
Cuts at: 3869 3869
Size: 5740
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3548 276
Size: 53 83 186 936 2014 2468
Fragments arranged by size:
2468 2014 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BstXI BstZ17I Bsu36I DraIII EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI