Restriction map for
pFN21AE9439
(Circular) (Six-base) MAPSORT of: pFN21AE9439.seq Check: 3374 from: 1 to: 6352
pFN21AE9439 6352 bp
With 182 enzymes: SgfI *
July 6, 2012 17:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2105 2350 2105
Size: 245 6107
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4162 278
Size: 53 83 186 936 2626 2468
Fragments arranged by size:
2626 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3513 3695 1937
Size: 1576 182 4594
Fragments arranged by size:
4594 1576 182
Acc65I G'GTAC_C
Cuts at: 3673 3673
Size: 6352
AclI AA'CG_TT
Cuts at: 1563 4479 4852 1563
Size: 2916 373 3063
Fragments arranged by size:
3063 2916 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2300 3164 4415 5799 6067 1632
Size: 668 864 1251 1384 268 1917
Fragments arranged by size:
1917 1384 1251 864 668 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6155
AflIII A'CryG_T
Cuts at: 1203 2115 5251 6228 1203
Size: 912 3136 977 1327
Fragments arranged by size:
3136 1327 977 912
AgeI A'CCGG_T
Cuts at: 5183 5183
Size: 6352
AhdI GACnn_n'nnGTC
Cuts at: 2061 5081 2061
Size: 3020 3332
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6352
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6352
AlwNI CAG_nnn'CTG
Cuts at: 4095 5667 4095
Size: 1572 4780
ApaI G_GGCC'C
Cuts at: 2032 2501 3025 2032
Size: 469 524 5359
Fragments arranged by size:
5359 524 469
ApaLI G'TGCA_C
Cuts at: 4409 5565 4409
Size: 1156 5196
ApoI r'AATT_y
Cuts at: 1493 3661 3887 4039 6148 1493
Size: 2168 226 152 2109 1697
Fragments arranged by size:
2168 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4906 160
Size: 872 3874 1606
Fragments arranged by size:
3874 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6352
AvaI C'yCGr_G
Cuts at: 1870 1943 2892 3024 3451 3677 1870
Size: 73 949 132 427 226 4545
Fragments arranged by size:
4545 949 427 226 132 73
BamHI G'GATC_C
Cuts at: 3682 4049 3682
Size: 367 5985
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3673 5128 618
Size: 325 217 627 1886 1455 1842
Fragments arranged by size:
1886 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2032 2343 2501 3025 3671 1391
Size: 641 311 158 524 646 4072
Fragments arranged by size:
4072 646 641 524 311 158
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2325 2599 2682 3028 3106 3541 928
Size: 971 426 274 83 346 78 435 3739
Fragments arranged by size:
3739 971 435 426 346 274 83 78
BbvCI CC'TCA_GC
Cuts at: 2300 2300
Size: 6352
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4543 4577 1903
Size: 34 2606 34 3678
Fragments arranged by size:
3678 2606 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4244 5454 4244
Size: 1210 5142
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6352
BfrBI ATG'CAT
Cuts at: 2572 2572
Size: 6352
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3020 3029 3159
Size: 107 122 71 631 605 1348 9 130
Cuts at: 3159 4963 136
Size: 1804 1525
Fragments arranged by size:
1804 1525 1348 631 605 130 122 107
71 9
BglII A'GATC_T
Cuts at: 3372 6347 3372
Size: 2975 3377
BlpI GC'TnA_GC
Cuts at: 2589 3149 3769 2589
Size: 560 620 5172
Fragments arranged by size:
5172 620 560
Bme1580I G_kGCm'C
Cuts at: 1790 2032 2501 3025 3135 4413 5569 1790
Size: 242 469 524 110 1278 1156 2573
Fragments arranged by size:
2573 1278 1156 524 469 242 110
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6352
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3204 4083 5041
Size: 416 53 83 399 24 1787 879 958
Cuts at: 5041 442
Size: 1753
Fragments arranged by size:
1787 1753 958 879 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6352
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2719 2719
Size: 6352
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2357 2618 2700 3275 5012 1239
Size: 1118 261 82 575 1737 2579
Fragments arranged by size:
2579 1737 1118 575 261 82
Bpu10I CC'TnA_GC
Cuts at: 2300 2300
Size: 6352
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3630 4477 5342 5640 3630
Size: 847 865 298 4342
Fragments arranged by size:
4342 865 847 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2767 2842 5015 882
Size: 617 1268 75 2173 2219
Fragments arranged by size:
2219 2173 1268 617 75
BsaAI yAC'GTr
Cuts at: 493 1204 2932 4143 493
Size: 711 1728 1211 2702
Fragments arranged by size:
2702 1728 1211 711
BsaBI GATnn'nnATC
Cuts at: 3185 6346 3185
Size: 3161 3191
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 4159 4541 5176
Size: 53 83 186 936 479 2147 382 635
Cuts at: 5176 275
Size: 1451
Fragments arranged by size:
2147 1451 936 635 479 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3420 4785 5183 5457 5604 6015 3420
Size: 1365 398 274 147 411 3757
Fragments arranged by size:
3757 1365 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6127
Fragments arranged by size:
6127 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 3595 1186
Size: 847 1562 3943
Fragments arranged by size:
3943 1562 847
BseYI C'CCAG_C
Cuts at: 3018 3162 3442 5555 6051 3018
Size: 144 280 2113 496 3319
Fragments arranged by size:
3319 2113 496 280 144
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2185 1163
Size: 573 449 5330
Fragments arranged by size:
5330 573 449
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2624 3802 4563 5591 1540
Size: 275 186 623 1178 761 1028 2301
Fragments arranged by size:
2301 1178 1028 761 623 275 186
BsiHKAI G_wGCw'C
Cuts at: 3435 3596 4413 4498 5569 3435
Size: 161 817 85 1071 4218
Fragments arranged by size:
4218 1071 817 161 85
BsmI GAATG_Cn'
Cuts at: 3863 3956 3863
Size: 93 6259
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2032 2343 2501 3025 3135 3435 3596
Size: 399 242 311 158 524 110 300 161
Cuts at: 3596 3671 4413 4498 5569 1391
Size: 75 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 524 399 311 300 242
161 158 110 85 75
BspEI T'CCGG_A
Cuts at: 6015 6015
Size: 6352
BspHI T'CATG_A
Cuts at: 4241 5971 4241
Size: 1730 4622
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2105 2350 3105 3707 844
Size: 1261 245 755 602 3489
Fragments arranged by size:
3489 1261 755 602 245
BsrBI CCG'CTC
Cuts at: 1394 1823 3409 4239 1394
Size: 429 1586 830 3507
Fragments arranged by size:
3507 1586 830 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2197 4847 5021 65
Size: 1183 949 2650 174 1396
Fragments arranged by size:
2650 1396 1183 949 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4996 5183 1700
Size: 110 3186 187 2869
Fragments arranged by size:
3186 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6352
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6352
BssSI C'ACGA_G
Cuts at: 4412 5424 4412
Size: 1012 5340
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6352
BstBI TT'CG_AA
Cuts at: 5156 5156
Size: 6352
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6352
BstXI CCAn_nnnn'nTGG
Cuts at: 2023 2023
Size: 6352
BstYI r'GATC_y
Cuts at: 1972 2925 3372 3682 4049 4434 4451 5903 5989
Size: 953 447 310 367 385 17 1452 86
Cuts at: 5989 6001 6347 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 953 447 385 367 346 310
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2059 2721 2059
Size: 662 5690
BtgI C'CryG_G
Cuts at: 513 1065 2004 2706 2793 3310 513
Size: 552 939 702 87 517 3555
Fragments arranged by size:
3555 939 702 552 517 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5613
Fragments arranged by size:
5613 683 56
BtsI GCAGTG_nn'
Cuts at: 2310 3868 4662 4682 2310
Size: 1558 794 20 3980
Fragments arranged by size:
3980 1558 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6352
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6317
DraI TTT'AAA
Cuts at: 3656 3656
Size: 6352
DraIII CAC_nnn'GTG
Cuts at: 2163 2163
Size: 6352
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5359 809
Size: 507 4043 1802
Fragments arranged by size:
4043 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2454 3480 3799
Size: 54 1425 50 275 18 624 1026 319
Cuts at: 3799 4688 6317 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1026 889 624 319 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3799 1537
Size: 275 1987 4090
Fragments arranged by size:
4090 1987 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2495 3613 4282 1353
Size: 1142 1118 669 3423
Fragments arranged by size:
3423 1142 1118 669
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4939 5313 5459 387
Size: 1064 138 3350 374 146 1280
Fragments arranged by size:
3350 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2300 2357 2618 2700 3164 3275 4415
Size: 393 668 57 261 82 464 111 1140
Cuts at: 4415 5012 5799 6067 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1140 787 668 597 464 393 268
261 111 82 57
EcoNI CCTnn'n_nnAGG
Cuts at: 2881 2881
Size: 6352
EcoO109I rG'GnC_Cy
Cuts at: 2020 2028 2029 2497 2498 3166 2020
Size: 8 1 468 1 668 5206
Fragments arranged by size:
5206 668 468 8 1 1
EcoRI G'AATT_C
Cuts at: 3661 4039 3661
Size: 378 5974
FspI TGC'GCA
Cuts at: 4858 4858
Size: 6352
HaeII r_GCGC'y
Cuts at: 1678 5499 1678
Size: 3821 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2047 2079 4993 5025 5067
Size: 32 135 32 475 32 2914 32 42
Cuts at: 5067 5099 1373
Size: 32 2626
Fragments arranged by size:
2914 2626 475 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3696 3942 669
Size: 988 281 1758 246 3079
Fragments arranged by size:
3079 1758 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6352
HpaI GTT'AAC
Cuts at: 3942 3942
Size: 6352
KpnI G_GTAC'C
Cuts at: 3677 3677
Size: 6352
MfeI C'AATT_G
Cuts at: 3951 3951
Size: 6352
MluI A'CGCG_T
Cuts at: 6228 6228
Size: 6352
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5466 5650 1305
Size: 4161 184 2007
Fragments arranged by size:
4161 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3482 10
Size: 54 1425 343 1650 2880
Fragments arranged by size:
2880 1650 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3068 4310 4669 4828 518
Size: 648 1902 1242 359 159 2042
Fragments arranged by size:
2042 1902 1242 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2526 3398 3768 4445 5593 5838 1606
Size: 920 872 370 677 1148 245 2120
Fragments arranged by size:
2120 1148 920 872 677 370 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4861
Fragments arranged by size:
4861 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6352
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6352
NotI GC'GGCC_GC
Cuts at: 3799 3799
Size: 6352
NsiI A_TGCA'T
Cuts at: 2574 2574
Size: 6352
NspI r_CATG'y
Cuts at: 1132 1229 2119 2576 3710 5255 1132
Size: 97 890 457 1134 1545 2229
Fragments arranged by size:
2229 1545 1134 890 457 97
PasI CC'CwG_GG
Cuts at: 1348 2016 2938 1348
Size: 668 922 4762
Fragments arranged by size:
4762 922 668
PciI A'CATG_T
Cuts at: 2115 5251 2115
Size: 3136 3216
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6352
PmeI GTTT'AAAC
Cuts at: 3656 3656
Size: 6352
PmlI CAC'GTG
Cuts at: 2932 2932
Size: 6352
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3233 3265 4421 4453 5934 5966 3233
Size: 32 1156 32 1481 32 3619
Fragments arranged by size:
3619 1481 1156 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2475 1613
Size: 862 5490
PsiI TTA'TAA
Cuts at: 3922 3922
Size: 6352
PspOMI G'GGCC_C
Cuts at: 2028 2497 3021 2028
Size: 469 524 5359
Fragments arranged by size:
5359 524 469
PstI C_TGCA'G
Cuts at: 830 2317 3704 830
Size: 1487 1387 3478
Fragments arranged by size:
3478 1487 1387
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6352
SalI G'TCGA_C
Cuts at: 1936 3694 1936
Size: 1758 4594
SbfI CC_TGCA'GG
Cuts at: 3704 3704
Size: 6352
ScaI AGT'ACT
Cuts at: 4600 6312 4600
Size: 1712 4640
SfcI C'TryA_G
Cuts at: 826 1045 2313 3393 3700 4835 5516 5707 826
Size: 219 1268 1080 307 1135 681 191 1471
Fragments arranged by size:
1471 1268 1135 1080 681 307 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6352
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6352
SmaI CCC'GGG
Cuts at: 1872 2894 3026 3679 1872
Size: 1022 132 653 4545
Fragments arranged by size:
4545 1022 653 132
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3451 3645 4456 5357 5619 820
Size: 197 926 1508 194 811 901 262 1553
Fragments arranged by size:
1553 1508 926 901 811 262 197 194
SnaBI TAC'GTA
Cuts at: 493 4143 493
Size: 3650 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6352
SphI G_CATG'C
Cuts at: 1132 3710 1132
Size: 2578 3774
SspI AAT'ATT
Cuts at: 5 52 4276 5
Size: 47 4224 2081
Fragments arranged by size:
4224 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6352
StyI C'CwwG_G
Cuts at: 513 1065 2004 2781 3791 513
Size: 552 939 777 1010 3074
Fragments arranged by size:
3074 1010 939 777 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2930 3452 4379 4396 4549 4734 5179
Size: 1255 1350 522 927 17 153 185 445
Cuts at: 5179 325
Size: 1498
Fragments arranged by size:
1498 1350 1255 927 522 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4598 6310 96
Size: 274 80 33 51 1443 2621 1712 138
Fragments arranged by size:
2621 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6312 6344 6312
Size: 32 6320
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6352
XbaI T'CTAG_A
Cuts at: 3688 3688
Size: 6352
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2828 2848 2828
Size: 20 6332
XhoI C'TCGA_G
Cuts at: 1943 3451 1943
Size: 1508 4844
XmaI C'CCGG_G
Cuts at: 1870 2892 3024 3677 1870
Size: 1022 132 653 4545
Fragments arranged by size:
4545 1022 653 132
XmnI GAAnn'nnTTC
Cuts at: 3374 4481 3374
Size: 1107 5245
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4160 276
Size: 53 83 186 936 2626 2468
Fragments arranged by size:
2626 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BsiWI BsmBI
BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PpuMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI