Restriction map for
pFN21AE9435
(Circular) (Six-base) MAPSORT of: pFN21AE9435.seq Check: 8595 from: 1 to: 6556
pFN21AE9435 6556 bp
With 182 enzymes: SgfI *
March 30, 2011 18:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2543 2543
Size: 6556
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4366 278
Size: 53 83 186 936 2830 2468
Fragments arranged by size:
2830 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2127 3315 3899 1937
Size: 190 1188 584 4594
Fragments arranged by size:
4594 1188 584 190
Acc65I G'GTAC_C
Cuts at: 3877 3877
Size: 6556
AclI AA'CG_TT
Cuts at: 1563 4683 5056 1563
Size: 3120 373 3063
Fragments arranged by size:
3120 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2407 3463 4619 6003 6271 1632
Size: 775 1056 1156 1384 268 1917
Fragments arranged by size:
1917 1384 1156 1056 775 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6359
AflIII A'CryG_T
Cuts at: 1203 2493 5455 6432 1203
Size: 1290 2962 977 1327
Fragments arranged by size:
2962 1327 1290 977
AgeI A'CCGG_T
Cuts at: 5387 5387
Size: 6556
AhdI GACnn_n'nnGTC
Cuts at: 5285 5285
Size: 6556
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6556
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6556
AlwNI CAG_nnn'CTG
Cuts at: 4299 5871 4299
Size: 1572 4984
ApaI G_GGCC'C
Cuts at: 2013 3717 2013
Size: 1704 4852
ApaLI G'TGCA_C
Cuts at: 4613 5769 4613
Size: 1156 5400
ApoI r'AATT_y
Cuts at: 1493 2185 2902 3865 4091 4243 6352 1493
Size: 692 717 963 226 152 2109 1697
Fragments arranged by size:
2109 1697 963 717 692 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5110 160
Size: 872 4078 1606
Fragments arranged by size:
4078 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6556
AvaI C'yCGr_G
Cuts at: 1870 1943 3638 3881 1870
Size: 73 1695 243 4545
Fragments arranged by size:
4545 1695 243 73
BamHI G'GATC_C
Cuts at: 3886 4253 3886
Size: 367 6189
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2504 2754 2943 3877 5332
Size: 325 217 627 717 250 189 934 1455
Cuts at: 5332 618
Size: 1842
Fragments arranged by size:
1842 1455 934 717 627 325 250 217
189
BanII G_rGCy'C
Cuts at: 1391 2013 2892 3549 3621 3717 3875 1391
Size: 622 879 657 72 96 158 4072
Fragments arranged by size:
4072 879 657 622 158 96 72
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2557 928
Size: 971 658 4927
Fragments arranged by size:
4927 971 658
BbvCI CC'TCA_GC
Cuts at: 2697 2697
Size: 6556
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2182 2216 2575 2609 3629 3663 4747
Size: 34 245 34 359 34 1020 34 1084
Cuts at: 4747 4781 1903
Size: 34 3678
Fragments arranged by size:
3678 1084 1020 359 245 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4448 5658 4448
Size: 1210 5346
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6556
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5167 136
Size: 107 122 71 631 605 3495 1525
Fragments arranged by size:
3495 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6551 6551
Size: 6556
BlpI GC'TnA_GC
Cuts at: 3543 3973 3543
Size: 430 6126
Bme1580I G_kGCm'C
Cuts at: 1790 2013 2759 3025 3061 3717 4617 5773 1790
Size: 223 746 266 36 656 900 1156 2573
Fragments arranged by size:
2573 1156 900 746 656 266 223 36
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6556
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4287 5245 442
Size: 416 53 83 399 24 2870 958 1753
Fragments arranged by size:
2870 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6556
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3712 3712
Size: 6556
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2390 3761 5216 1239
Size: 1151 1371 1455 2579
Fragments arranged by size:
2579 1455 1371 1151
Bpu10I CC'TnA_GC
Cuts at: 2697 3162 3321 3591 3615 2697
Size: 465 159 270 24 5638
Fragments arranged by size:
5638 465 270 159 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3242 4681 5546 5844 3242
Size: 1439 865 298 3954
Fragments arranged by size:
3954 1439 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5219 882
Size: 617 3720 2219
Fragments arranged by size:
3720 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2629 4347 493
Size: 711 1425 1718 2702
Fragments arranged by size:
2702 1718 1425 711
BsaBI GATnn'nnATC
Cuts at: 2111 6550 2111
Size: 4439 2117
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4363 4745 5380 275
Size: 53 83 186 936 2830 382 635 1451
Fragments arranged by size:
2830 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2181 3099 4989 5387 5661 5808 6219 2181
Size: 918 1890 398 274 147 411 2518
Fragments arranged by size:
2518 1890 918 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6331
Fragments arranged by size:
6331 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2597 2726 2882 3172 1186
Size: 1411 129 156 290 4570
Fragments arranged by size:
4570 1411 290 156 129
BseYI C'CCAG_C
Cuts at: 2061 2283 3710 5759 6255 2061
Size: 222 1427 2049 496 2362
Fragments arranged by size:
2362 2049 1427 496 222
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5983
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3145 4006 4767 5795 1540
Size: 275 186 1144 861 761 1028 2301
Fragments arranged by size:
2301 1144 1028 861 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2772 3621 4617 4702 5773 2772
Size: 849 996 85 1071 3555
Fragments arranged by size:
3555 1071 996 849 85
BsmI GAATG_Cn'
Cuts at: 4067 4160 4067
Size: 93 6463
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2759 2772 2892 3025 3061 3549
Size: 399 223 746 13 120 133 36 488
Cuts at: 3549 3621 3717 3875 4617 4702 5773 1391
Size: 72 96 158 742 85 1071 2174
Fragments arranged by size:
2174 1071 746 742 488 399 223 158
133 120 96 85 72 36 13
BspEI T'CCGG_A
Cuts at: 2181 3099 6219 2181
Size: 918 3120 2518
Fragments arranged by size:
3120 2518 918
BspHI T'CATG_A
Cuts at: 2466 4445 6175 2466
Size: 1979 1730 2847
Fragments arranged by size:
2847 1979 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2447 2543 3911 844
Size: 1603 96 1368 3489
Fragments arranged by size:
3489 1603 1368 96
BsrBI CCG'CTC
Cuts at: 1394 1823 4443 1394
Size: 429 2620 3507
Fragments arranged by size:
3507 2620 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3631 3651 5051 5225 65
Size: 1183 2383 20 1400 174 1396
Fragments arranged by size:
2383 1400 1396 1183 174 20
BsrFI r'CCGG_y
Cuts at: 1700 1810 2077 5200 5387 1700
Size: 110 267 3123 187 2869
Fragments arranged by size:
3123 2869 267 187 110
BsrGI T'GTAC_A
Cuts at: 96 2496 96
Size: 2400 4156
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6556
BssSI C'ACGA_G
Cuts at: 3042 4616 5628 3042
Size: 1574 1012 3970
Fragments arranged by size:
3970 1574 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2439 794
Size: 1645 4911
BstBI TT'CG_AA
Cuts at: 5360 5360
Size: 6556
BstEII G'GTnAC_C
Cuts at: 1184 3251 3293 1184
Size: 2067 42 4447
Fragments arranged by size:
4447 2067 42
BstXI CCAn_nnnn'nTGG
Cuts at: 2650 3739 2650
Size: 1089 5467
BstYI r'GATC_y
Cuts at: 1972 2287 2418 2634 2814 3176 3642 3886 4253
Size: 315 131 216 180 362 466 244 367
Cuts at: 4253 4638 4655 6107 6193 6205 6551 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 466 385 367 362 346 315
244 216 180 131 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2145 513
Size: 552 939 141 4924
Fragments arranged by size:
4924 939 552 141
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2120 3739 487
Size: 683 56 894 1619 3304
Fragments arranged by size:
3304 1619 894 683 56
BtsI GCAGTG_nn'
Cuts at: 2751 3552 3597 4072 4866 4886 2751
Size: 801 45 475 794 20 4421
Fragments arranged by size:
4421 801 794 475 45 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6556
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6521
DraI TTT'AAA
Cuts at: 3860 3860
Size: 6556
DraIII CAC_nnn'GTG
Cuts at: 3664 3664
Size: 6556
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5563 809
Size: 507 4247 1802
Fragments arranged by size:
4247 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2220 2235 3133
Size: 54 1425 50 275 18 390 15 898
Cuts at: 3133 3154 3271 3439 3514 4003 4892 6521 8
Size: 21 117 168 75 489 889 1629 43
Fragments arranged by size:
1629 1425 898 889 489 390 275 168
117 75 54 50 43 21 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 4003 1537
Size: 275 2191 4090
Fragments arranged by size:
4090 2191 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2905 4486 1353
Size: 1552 1581 3423
Fragments arranged by size:
3423 1581 1552
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5143 5517 5663 387
Size: 1064 138 3554 374 146 1280
Fragments arranged by size:
3554 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3619 3619
Size: 6556
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2390 2407 3463 3761 4619 5216 6003
Size: 393 758 17 1056 298 858 597 787
Cuts at: 6003 6271 1239
Size: 268 1524
Fragments arranged by size:
1524 1056 858 787 758 597 393 298
268 17
EcoO109I rG'GnC_Cy
Cuts at: 2009 2009
Size: 6556
EcoRI G'AATT_C
Cuts at: 2185 3865 4243 2185
Size: 1680 378 4498
Fragments arranged by size:
4498 1680 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3241 3493 3241
Size: 252 6304
FspI TGC'GCA
Cuts at: 2432 5062 2432
Size: 2630 3926
HaeII r_GCGC'y
Cuts at: 1678 5703 1678
Size: 4025 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3134 3166 5197 5229 5271
Size: 32 135 32 1562 32 2031 32 42
Cuts at: 5271 5303 1373
Size: 32 2626
Fragments arranged by size:
2626 2031 1562 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3900 4146 669
Size: 988 281 1962 246 3079
Fragments arranged by size:
3079 1962 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6556
HpaI GTT'AAC
Cuts at: 4146 4146
Size: 6556
KpnI G_GTAC'C
Cuts at: 3881 3881
Size: 6556
MfeI C'AATT_G
Cuts at: 4155 4155
Size: 6556
MluI A'CGCG_T
Cuts at: 6432 6432
Size: 6556
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5670 5854 1305
Size: 4365 184 2007
Fragments arranged by size:
4365 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2237 3135 3156 3273 3441
Size: 54 1425 343 405 898 21 117 168
Cuts at: 3441 3516 10
Size: 75 3050
Fragments arranged by size:
3050 1425 898 405 343 168 117 75
54 21
MslI CAynn'nnrTG
Cuts at: 518 1166 2093 2201 2723 4514 4873 5032 518
Size: 648 927 108 522 1791 359 159 2042
Fragments arranged by size:
2042 1791 927 648 522 359 159 108
MspA1I CmG'CkG
Cuts at: 1606 2865 3131 3710 3739 3972 4649 5797 6042
Size: 1259 266 579 29 233 677 1148 245
Cuts at: 6042 1606
Size: 2120
Fragments arranged by size:
2120 1259 1148 677 579 266 245 233
29
NcoI C'CATG_G
Cuts at: 513 1065 2004 2145 513
Size: 552 939 141 4924
Fragments arranged by size:
4924 939 552 141
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6556
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6556
NotI GC'GGCC_GC
Cuts at: 4003 4003
Size: 6556
NruI TCG'CGA
Cuts at: 3033 3033
Size: 6556
NspI r_CATG'y
Cuts at: 1132 1229 2267 2497 3914 5459 1132
Size: 97 1038 230 1417 1545 2229
Fragments arranged by size:
2229 1545 1417 1038 230 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6556
PciI A'CATG_T
Cuts at: 2493 5455 2493
Size: 2962 3594
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3116 1108
Size: 2008 4548
PmeI GTTT'AAAC
Cuts at: 3860 3860
Size: 6556
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4625 4657 6138 6170 4625
Size: 32 1481 32 5011
Fragments arranged by size:
5011 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6556
PsiI TTA'TAA
Cuts at: 4126 4126
Size: 6556
PspOMI G'GGCC_C
Cuts at: 2009 3713 2009
Size: 1704 4852
PstI C_TGCA'G
Cuts at: 830 2671 3130 3908 830
Size: 1841 459 778 3478
Fragments arranged by size:
3478 1841 778 459
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6556
PvuII CAG'CTG
Cuts at: 2865 3131 3739 2865
Size: 266 608 5682
Fragments arranged by size:
5682 608 266
SacI G_AGCT'C
Cuts at: 3621 3621
Size: 6556
SalI G'TCGA_C
Cuts at: 1936 3898 1936
Size: 1962 4594
SbfI CC_TGCA'GG
Cuts at: 2671 3908 2671
Size: 1237 5319
ScaI AGT'ACT
Cuts at: 4804 6516 4804
Size: 1712 4844
SexAI A'CCwGG_T
Cuts at: 2448 3115 2448
Size: 667 5889
SfcI C'TryA_G
Cuts at: 826 1045 2667 3126 3777 3904 5039 5720 5911
Size: 219 1622 459 651 127 1135 681 191
Cuts at: 5911 826
Size: 1471
Fragments arranged by size:
1622 1471 1135 681 651 459 219 191
127
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6556
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6556
SmaI CCC'GGG
Cuts at: 1872 3883 1872
Size: 2011 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3257 4660 5561 5823 820
Size: 197 926 1314 1403 901 262 1553
Fragments arranged by size:
1553 1403 1314 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4347 493
Size: 3854 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6556
SphI G_CATG'C
Cuts at: 1132 2267 3914 1132
Size: 1135 1647 3774
Fragments arranged by size:
3774 1647 1135
SspI AAT'ATT
Cuts at: 5 52 4480 5
Size: 47 4428 2081
Fragments arranged by size:
4428 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6556
StyI C'CwwG_G
Cuts at: 513 1065 2004 2145 3237 3995 513
Size: 552 939 141 1092 758 3074
Fragments arranged by size:
3074 1092 939 758 552 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4583 4600 4753 4938 5383 325
Size: 1255 3003 17 153 185 445 1498
Fragments arranged by size:
3003 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2496 4802 6514
Size: 274 80 33 51 1443 519 2306 1712
Cuts at: 6514 96
Size: 138
Fragments arranged by size:
2306 1712 1443 519 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2410 2442 6516 6548 2410
Size: 32 4074 32 2418
Fragments arranged by size:
4074 2418 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3353 3374 1316
Size: 2037 21 4498
Fragments arranged by size:
4498 2037 21
XbaI T'CTAG_A
Cuts at: 3892 3892
Size: 6556
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2152 3452 2152
Size: 1300 5256
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6556
XmaI C'CCGG_G
Cuts at: 1870 3881 1870
Size: 2011 4545
XmnI GAAnn'nnTTC
Cuts at: 4685 4685
Size: 6556
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4364 276
Size: 53 83 186 936 2830 2468
Fragments arranged by size:
2830 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NruI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NsiI PacI PfoI PmlI PpuMI
PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI
SrfI SwaI