Restriction map for
pFN21AE9429
(Circular) (Six-base) MAPSORT of: pFN21AE9429.seq Check: 2723 from: 1 to: 5764
pFN21AE9429 5764 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2477 2477
Size: 5764
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3574 278
Size: 53 83 186 936 2038 2468
Fragments arranged by size:
2468 2038 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2097 2577 3107 1937
Size: 160 480 530 4594
Fragments arranged by size:
4594 530 480 160
Acc65I G'GTAC_C
Cuts at: 3085 3085
Size: 5764
AclI AA'CG_TT
Cuts at: 1563 3891 4264 1563
Size: 2328 373 3063
Fragments arranged by size:
3063 2328 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2538 3827 5211 5479 1632
Size: 906 1289 1384 268 1917
Fragments arranged by size:
1917 1384 1289 906 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5567
AflIII A'CryG_T
Cuts at: 1203 4663 5640 1203
Size: 3460 977 1327
Fragments arranged by size:
3460 1327 977
AgeI A'CCGG_T
Cuts at: 4595 4595
Size: 5764
AhdI GACnn_n'nnGTC
Cuts at: 4493 4493
Size: 5764
AleI CACnn'nnGTG
Cuts at: 1166 2537 1166
Size: 1371 4393
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2555 3029 1184
Size: 1371 474 3919
Fragments arranged by size:
3919 1371 474
AlwNI CAG_nnn'CTG
Cuts at: 3507 5079 3507
Size: 1572 4192
ApaI G_GGCC'C
Cuts at: 2731 2731
Size: 5764
ApaLI G'TGCA_C
Cuts at: 3821 4977 3821
Size: 1156 4608
ApoI r'AATT_y
Cuts at: 1493 2327 3073 3299 3451 5560 1493
Size: 834 746 226 152 2109 1697
Fragments arranged by size:
2109 1697 834 746 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4318 160
Size: 872 3286 1606
Fragments arranged by size:
3286 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5764
AvaI C'yCGr_G
Cuts at: 1870 1943 3089 1870
Size: 73 1146 4545
Fragments arranged by size:
4545 1146 73
BamHI G'GATC_C
Cuts at: 2559 3094 3461 2559
Size: 535 367 4862
Fragments arranged by size:
4862 535 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2584 2767 3085 4540 618
Size: 325 217 627 797 183 318 1455 1842
Fragments arranged by size:
1842 1455 797 627 325 318 217 183
BanII G_rGCy'C
Cuts at: 1391 2731 3083 1391
Size: 1340 352 4072
Fragments arranged by size:
4072 1340 352
BbeI G_GCGC'C
Cuts at: 2771 2771
Size: 5764
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2182 2526 2684 928
Size: 971 283 344 158 4008
Fragments arranged by size:
4008 971 344 283 158
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3955 3989 1903
Size: 34 2018 34 3678
Fragments arranged by size:
3678 2018 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3067 3656 4866 3067
Size: 589 1210 3965
Fragments arranged by size:
3965 1210 589
BclI T'GATC_A
Cuts at: 1548 2148 1548
Size: 600 5164
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4375 136
Size: 107 122 71 631 605 2703 1525
Fragments arranged by size:
2703 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5759 5759
Size: 5764
BlpI GC'TnA_GC
Cuts at: 3181 3181
Size: 5764
Bme1580I G_kGCm'C
Cuts at: 1790 2091 2589 2731 3825 4981 1790
Size: 301 498 142 1094 1156 2573
Fragments arranged by size:
2573 1156 1094 498 301 142
BmgBI CAC'GTC
Cuts at: 1318 2548 1318
Size: 1230 4534
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3495 4453 442
Size: 416 53 83 399 24 2078 958 1753
Fragments arranged by size:
2078 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2825 1056
Size: 1769 3995
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4424 1239
Size: 3185 2579
Bpu10I CC'TnA_GC
Cuts at: 2633 2633
Size: 5764
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2390 2888 3889 4754 5052 2390
Size: 498 1001 865 298 3102
Fragments arranged by size:
3102 1001 865 498 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2425 4427 882
Size: 617 926 2002 2219
Fragments arranged by size:
2219 2002 926 617
BsaAI yAC'GTr
Cuts at: 493 1204 3555 493
Size: 711 2351 2702
Fragments arranged by size:
2702 2351 711
BsaBI GATnn'nnATC
Cuts at: 5758 5758
Size: 5764
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2768 3571 3953 4588
Size: 53 83 186 936 1235 803 382 635
Cuts at: 4588 275
Size: 1451
Fragments arranged by size:
1451 1235 936 803 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4197 4595 4869 5016 5427 4197
Size: 398 274 147 411 4534
Fragments arranged by size:
4534 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5539
Fragments arranged by size:
5539 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5764
BseYI C'CCAG_C
Cuts at: 2160 2428 4967 5463 2160
Size: 268 2539 496 2461
Fragments arranged by size:
2539 2461 496 268
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5191
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3214 3975 5003 1540
Size: 275 186 1213 761 1028 2301
Fragments arranged by size:
2301 1213 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2013 3825 3910 4981 2013
Size: 1812 85 1071 2796
Fragments arranged by size:
2796 1812 1071 85
BsmI GAATG_Cn'
Cuts at: 3275 3368 3275
Size: 93 5671
BsmBI CGTCTCn'nnnn_
Cuts at: 2660 2660
Size: 5764
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2091 2589 2731 3083 3825 3910
Size: 399 223 78 498 142 352 742 85
Cuts at: 3910 4981 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 498 399 352 223 142
85 78
BspEI T'CCGG_A
Cuts at: 5427 5427
Size: 5764
BspHI T'CATG_A
Cuts at: 2742 3653 5383 2742
Size: 911 1730 3123
Fragments arranged by size:
3123 1730 911
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2477 3119 844
Size: 1633 642 3489
Fragments arranged by size:
3489 1633 642
BsrBI CCG'CTC
Cuts at: 1394 1823 2404 3651 1394
Size: 429 581 1247 3507
Fragments arranged by size:
3507 1247 581 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2808 4259 4433 65
Size: 1183 1560 1451 174 1396
Fragments arranged by size:
1560 1451 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2989 4408 4595 1700
Size: 110 1179 1419 187 2869
Fragments arranged by size:
2869 1419 1179 187 110
BsrGI T'GTAC_A
Cuts at: 96 2625 2935 96
Size: 2529 310 2925
Fragments arranged by size:
2925 2529 310
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5764
BssSI C'ACGA_G
Cuts at: 3824 4836 3824
Size: 1012 4752
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2213 794
Size: 1419 4345
BstBI TT'CG_AA
Cuts at: 4568 4568
Size: 5764
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5764
BstXI CCAn_nnnn'nTGG
Cuts at: 2762 2762
Size: 5764
BstYI r'GATC_y
Cuts at: 1972 2559 2982 3094 3461 3846 3863 5315 5401
Size: 587 423 112 367 385 17 1452 86
Cuts at: 5401 5413 5759 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 587 423 385 367 346 112
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2295 2295
Size: 5764
BtgI C'CryG_G
Cuts at: 513 1065 2004 2091 2659 2670 2886 513
Size: 552 939 87 568 11 216 3391
Fragments arranged by size:
3391 939 568 552 216 87 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5025
Fragments arranged by size:
5025 683 56
BtsI GCAGTG_nn'
Cuts at: 2563 3001 3280 4074 4094 2563
Size: 438 279 794 20 4233
Fragments arranged by size:
4233 794 438 279 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5764
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5729
DraI TTT'AAA
Cuts at: 3068 3068
Size: 5764
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2228 4771 809
Size: 507 912 2543 1802
Fragments arranged by size:
2543 1802 912 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3211 4100 5729
Size: 54 1425 50 275 18 1381 889 1629
Cuts at: 5729 8
Size: 43
Fragments arranged by size:
1629 1425 1381 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3211 1537
Size: 275 1399 4090
Fragments arranged by size:
4090 1399 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2636 3694 1353
Size: 1283 1058 3423
Fragments arranged by size:
3423 1283 1058
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4351 4725 4871 387
Size: 1064 138 2762 374 146 1280
Fragments arranged by size:
2762 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2538 3827 4424 5211 5479 1239
Size: 393 906 1289 597 787 268 1524
Fragments arranged by size:
1524 1289 906 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2299 2728 2299
Size: 429 5335
EcoRI G'AATT_C
Cuts at: 2327 3073 3451 2327
Size: 746 378 4640
Fragments arranged by size:
4640 746 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2929 2929
Size: 5764
FspI TGC'GCA
Cuts at: 4270 4270
Size: 5764
HaeII r_GCGC'y
Cuts at: 1678 2771 4911 1678
Size: 1093 2140 2531
Fragments arranged by size:
2531 2140 1093
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4405 4437 4479 4511 1373
Size: 32 135 32 2833 32 42 32 2626
Fragments arranged by size:
2833 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2370 3108 3354 669
Size: 988 281 432 738 246 3079
Fragments arranged by size:
3079 988 738 432 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5764
HpaI GTT'AAC
Cuts at: 2370 3354 2370
Size: 984 4780
KasI G'GCGC_C
Cuts at: 2767 2767
Size: 5764
KpnI G_GTAC'C
Cuts at: 3089 3089
Size: 5764
MfeI C'AATT_G
Cuts at: 3363 3363
Size: 5764
MluI A'CGCG_T
Cuts at: 5640 5640
Size: 5764
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2244 2829 4878 5062 1305
Size: 939 585 2049 184 2007
Fragments arranged by size:
2049 2007 939 585 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3942
Fragments arranged by size:
3942 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2288 2318 2537 2741 2760 3011 3722
Size: 648 1122 30 219 204 19 251 711
Cuts at: 3722 4081 4240 518
Size: 359 159 2042
Fragments arranged by size:
2042 1122 711 648 359 251 219 204
159 30 19
MspA1I CmG'CkG
Cuts at: 1606 2164 2784 3180 3857 5005 5250 1606
Size: 558 620 396 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 620 558 396 245
NaeI GCC'GGC
Cuts at: 2991 2991
Size: 5764
NarI GG'CG_CC
Cuts at: 2768 2768
Size: 5764
NcoI C'CATG_G
Cuts at: 513 1065 2004 2886 513
Size: 552 939 882 3391
Fragments arranged by size:
3391 939 882 552
NdeI CA'TA_TG
Cuts at: 387 2351 387
Size: 1964 3800
NgoMIV G'CCGG_C
Cuts at: 2989 2989
Size: 5764
NheI G'CTAG_C
Cuts at: 1052 2821 1052
Size: 1769 3995
NotI GC'GGCC_GC
Cuts at: 3211 3211
Size: 5764
NspI r_CATG'y
Cuts at: 1132 1229 2287 2821 3122 4667 1132
Size: 97 1058 534 301 1545 2229
Fragments arranged by size:
2229 1545 1058 534 301 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5764
PciI A'CATG_T
Cuts at: 4663 4663
Size: 5764
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2167 2511 2724 1108
Size: 1059 344 213 4148
Fragments arranged by size:
4148 1059 344 213
PmeI GTTT'AAAC
Cuts at: 3068 3068
Size: 5764
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3833 3865 5346 5378 3833
Size: 32 1481 32 4219
Fragments arranged by size:
4219 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2299 2299
Size: 5764
PshAI GACnn'nnGTC
Cuts at: 1613 2182 1613
Size: 569 5195
PsiI TTA'TAA
Cuts at: 3334 3334
Size: 5764
PspOMI G'GGCC_C
Cuts at: 2727 2727
Size: 5764
PstI C_TGCA'G
Cuts at: 830 3116 830
Size: 2286 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5764
PvuII CAG'CTG
Cuts at: 2164 2164
Size: 5764
SalI G'TCGA_C
Cuts at: 1936 3106 1936
Size: 1170 4594
SapI GCTCTTCn'nnn_
Cuts at: 2636 2636
Size: 5764
SbfI CC_TGCA'GG
Cuts at: 3116 3116
Size: 5764
ScaI AGT'ACT
Cuts at: 4012 5724 4012
Size: 1712 4052
SexAI A'CCwGG_T
Cuts at: 2580 2580
Size: 5764
SfcI C'TryA_G
Cuts at: 826 1045 2101 3112 4247 4928 5119 826
Size: 219 1056 1011 1135 681 191 1471
Fragments arranged by size:
1471 1135 1056 1011 681 219 191
SfoI GGC'GCC
Cuts at: 2769 2769
Size: 5764
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5764
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5764
SmaI CCC'GGG
Cuts at: 1872 3091 1872
Size: 1219 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2405 2903 3868 4769 5031 820
Size: 197 926 462 498 965 901 262 1553
Fragments arranged by size:
1553 965 926 901 498 462 262 197
SnaBI TAC'GTA
Cuts at: 493 3555 493
Size: 3062 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5764
SphI G_CATG'C
Cuts at: 1132 3122 1132
Size: 1990 3774
SspI AAT'ATT
Cuts at: 5 52 3688 5
Size: 47 3636 2081
Fragments arranged by size:
3636 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5764
StyI C'CwwG_G
Cuts at: 513 1065 2004 2886 3203 513
Size: 552 939 882 317 3074
Fragments arranged by size:
3074 939 882 552 317
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3791 3808 3961 4146 4591 325
Size: 1255 2211 17 153 185 445 1498
Fragments arranged by size:
2211 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2625 2935 4010
Size: 274 80 33 51 1443 648 310 1075
Cuts at: 4010 5722 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1075 648 310 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2786 2818 5724 5756 2786
Size: 32 2906 32 2794
Fragments arranged by size:
2906 2794 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5764
XbaI T'CTAG_A
Cuts at: 3100 3100
Size: 5764
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5764
XmaI C'CCGG_G
Cuts at: 1870 3089 1870
Size: 1219 4545
XmnI GAAnn'nnTTC
Cuts at: 3893 3893
Size: 5764
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3572 276
Size: 53 83 186 936 2038 2468
Fragments arranged by size:
2468 2038 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI
NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII
SacI SacII SanDI SfiI SrfI SwaI XcmI