Restriction map for
pFN21AE9428
(Circular) (Six-base) MAPSORT of: pFN21AE9428.seq Check: 527 from: 1 to: 5902
pFN21AE9428 5902 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2407 2407
Size: 5902
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3712 278
Size: 53 83 186 936 2176 2468
Fragments arranged by size:
2468 2176 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2148 3245 1937
Size: 211 1097 4594
Fragments arranged by size:
4594 1097 211
Acc65I G'GTAC_C
Cuts at: 3223 3223
Size: 5902
AclI AA'CG_TT
Cuts at: 1563 4029 4402 1563
Size: 2466 373 3063
Fragments arranged by size:
3063 2466 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3071 3965 5349 5617 1632
Size: 1439 894 1384 268 1917
Fragments arranged by size:
1917 1439 1384 894 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5705
AflIII A'CryG_T
Cuts at: 1203 4801 5778 1203
Size: 3598 977 1327
Fragments arranged by size:
3598 1327 977
AgeI A'CCGG_T
Cuts at: 2466 4733 2466
Size: 2267 3635
AhdI GACnn_n'nnGTC
Cuts at: 4631 4631
Size: 5902
AleI CACnn'nnGTG
Cuts at: 1166 2417 1166
Size: 1251 4651
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5902
AlwNI CAG_nnn'CTG
Cuts at: 3645 5217 3645
Size: 1572 4330
ApaLI G'TGCA_C
Cuts at: 3959 5115 3959
Size: 1156 4746
ApoI r'AATT_y
Cuts at: 1493 2521 3211 3437 3589 5698 1493
Size: 1028 690 226 152 2109 1697
Fragments arranged by size:
2109 1697 1028 690 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2595 4456 160
Size: 872 1563 1861 1606
Fragments arranged by size:
1861 1606 1563 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5902
AvaI C'yCGr_G
Cuts at: 1870 1943 2012 2751 3227 1870
Size: 73 69 739 476 4545
Fragments arranged by size:
4545 739 476 73 69
BamHI G'GATC_C
Cuts at: 3178 3232 3599 3178
Size: 54 367 5481
Fragments arranged by size:
5481 367 54
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3223 4678 618
Size: 325 217 627 1436 1455 1842
Fragments arranged by size:
1842 1455 1436 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2696 3221 1391
Size: 1305 525 4072
Fragments arranged by size:
4072 1305 525
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2236 2335 928
Size: 971 337 99 4495
Fragments arranged by size:
4495 971 337 99
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4093 4127 1903
Size: 34 2156 34 3678
Fragments arranged by size:
3678 2156 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2867 3794 5004 2867
Size: 927 1210 3765
Fragments arranged by size:
3765 1210 927
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5902
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4513 136
Size: 107 122 71 631 605 2841 1525
Fragments arranged by size:
2841 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3011 5897 3011
Size: 2886 3016
BlpI GC'TnA_GC
Cuts at: 2228 2330 3319 2228
Size: 102 989 4811
Fragments arranged by size:
4811 989 102
Bme1580I G_kGCm'C
Cuts at: 1790 2118 3963 5119 1790
Size: 328 1845 1156 2573
Fragments arranged by size:
2573 1845 1156 328
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5902
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3633 4591 442
Size: 416 53 83 399 24 2216 958 1753
Fragments arranged by size:
2216 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5902
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2433 3087 4562 1239
Size: 1194 654 1475 2579
Fragments arranged by size:
2579 1475 1194 654
Bpu10I CC'TnA_GC
Cuts at: 2665 2665
Size: 5902
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4027 4892 5190 4027
Size: 865 298 4739
Fragments arranged by size:
4739 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2048 4565 882
Size: 617 549 2517 2219
Fragments arranged by size:
2517 2219 617 549
BsaAI yAC'GTr
Cuts at: 493 1204 2125 2188 2878 3693 493
Size: 711 921 63 690 815 2702
Fragments arranged by size:
2702 921 815 711 690 63
BsaBI GATnn'nnATC
Cuts at: 5896 5896
Size: 5902
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3709 4091 4726 275
Size: 53 83 186 936 2176 382 635 1451
Fragments arranged by size:
2176 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2466 4335 4733 5007 5154 5565 2466
Size: 1869 398 274 147 411 2803
Fragments arranged by size:
2803 1869 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2471 2501 1178
Size: 30 165 30 1068 30 4579
Fragments arranged by size:
4579 1068 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2686 1186
Size: 1500 4402
BseYI C'CCAG_C
Cuts at: 2071 2860 2990 5105 5601 2071
Size: 789 130 2115 496 2372
Fragments arranged by size:
2372 2115 789 496 130
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2210 1163
Size: 573 474 4855
Fragments arranged by size:
4855 573 474
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2027 2112 3352 4113 5141 1540
Size: 275 186 26 85 1240 761 1028 2301
Fragments arranged by size:
2301 1240 1028 761 275 186 85 26
BsiHKAI G_wGCw'C
Cuts at: 3963 4048 5119 3963
Size: 85 1071 4746
Fragments arranged by size:
4746 1071 85
BsmI GAATG_Cn'
Cuts at: 3413 3506 3413
Size: 93 5809
BsmBI CGTCTCn'nnnn_
Cuts at: 2581 2629 2581
Size: 48 5854
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2118 2696 3221 3963 4048 5119 1391
Size: 399 328 578 525 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 578 525 399 328 85
BspEI T'CCGG_A
Cuts at: 5565 5565
Size: 5902
BspHI T'CATG_A
Cuts at: 3791 5521 3791
Size: 1730 4172
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2407 3257 844
Size: 1563 850 3489
Fragments arranged by size:
3489 1563 850
BsrBI CCG'CTC
Cuts at: 1394 1823 3789 1394
Size: 429 1966 3507
Fragments arranged by size:
3507 1966 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3069 4397 4571 65
Size: 1183 1821 1328 174 1396
Fragments arranged by size:
1821 1396 1328 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2466 4546 4733 1700
Size: 110 656 2080 187 2869
Fragments arranged by size:
2869 2080 656 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5902
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5902
BssSI C'ACGA_G
Cuts at: 3962 4974 3962
Size: 1012 4890
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5902
BstBI TT'CG_AA
Cuts at: 4706 4706
Size: 5902
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5902
BstYI r'GATC_y
Cuts at: 1972 3011 3178 3232 3599 3984 4001 5453 5539
Size: 1039 167 54 367 385 17 1452 86
Cuts at: 5539 5551 5897 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1039 385 367 346 167 86
54 17 12
Bsu36I CC'TnA_GG
Cuts at: 3078 3078
Size: 5902
BtgI C'CryG_G
Cuts at: 513 1065 2063 2995 513
Size: 552 998 932 3420
Fragments arranged by size:
3420 998 932 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5163
Fragments arranged by size:
5163 683 56
BtsI GCAGTG_nn'
Cuts at: 3418 4212 4232 3418
Size: 794 20 5088
Fragments arranged by size:
5088 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5902
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5867
DraI TTT'AAA
Cuts at: 3206 3206
Size: 5902
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4909 809
Size: 507 3593 1802
Fragments arranged by size:
3593 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2024 2109 2319
Size: 54 1425 50 275 18 194 85 210
Cuts at: 2319 2589 3349 4238 5867 8
Size: 270 760 889 1629 43
Fragments arranged by size:
1629 1425 889 760 275 270 210 194
85 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2024 2109 3349 1537
Size: 275 212 85 1240 4090
Fragments arranged by size:
4090 1240 275 212 85
EarI CTCTTCn'nnn_
Cuts at: 1353 2177 2267 2294 2498 2612 3832 1353
Size: 824 90 27 204 114 1220 3423
Fragments arranged by size:
3423 1220 824 204 114 90 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4489 4863 5009 387
Size: 1064 138 2900 374 146 1280
Fragments arranged by size:
2900 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2433 3071 3087 3965 4562 5349 5617
Size: 393 801 638 16 878 597 787 268
Cuts at: 5617 1239
Size: 1524
Fragments arranged by size:
1524 878 801 787 638 597 393 268
16
EcoO109I rG'GnC_Cy
Cuts at: 2068 2965 2068
Size: 897 5005
EcoRI G'AATT_C
Cuts at: 3211 3589 3211
Size: 378 5524
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2680 2680
Size: 5902
FspI TGC'GCA
Cuts at: 4408 4408
Size: 5902
HaeII r_GCGC'y
Cuts at: 1678 2040 5049 1678
Size: 362 3009 2531
Fragments arranged by size:
3009 2531 362
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4543 4575 4617 4649 1373
Size: 32 135 32 2971 32 42 32 2626
Fragments arranged by size:
2971 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3246 3492 669
Size: 988 281 1308 246 3079
Fragments arranged by size:
3079 1308 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2129 748
Size: 1381 4521
HpaI GTT'AAC
Cuts at: 3492 3492
Size: 5902
KpnI G_GTAC'C
Cuts at: 3227 3227
Size: 5902
MfeI C'AATT_G
Cuts at: 3501 3501
Size: 5902
MluI A'CGCG_T
Cuts at: 5778 5778
Size: 5902
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5016 5200 1305
Size: 3711 184 2007
Fragments arranged by size:
3711 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2591 10
Size: 54 1425 343 759 3321
Fragments arranged by size:
3321 1425 759 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2186 2396 2417 3860 4219 4378 518
Size: 648 1020 210 21 1443 359 159 2042
Fragments arranged by size:
2042 1443 1020 648 359 210 159 21
MspA1I CmG'CkG
Cuts at: 1606 2860 3318 3995 5143 5388 1606
Size: 1254 458 677 1148 245 2120
Fragments arranged by size:
2120 1254 1148 677 458 245
NcoI C'CATG_G
Cuts at: 513 1065 2995 513
Size: 552 1930 3420
Fragments arranged by size:
3420 1930 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5902
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5902
NotI GC'GGCC_GC
Cuts at: 3349 3349
Size: 5902
NspI r_CATG'y
Cuts at: 1132 1229 3260 4805 1132
Size: 97 2031 1545 2229
Fragments arranged by size:
2229 2031 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5902
PciI A'CATG_T
Cuts at: 4801 4801
Size: 5902
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3197 1108
Size: 2089 3813
PmeI GTTT'AAAC
Cuts at: 3206 3206
Size: 5902
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3971 4003 5484 5516 3971
Size: 32 1481 32 4357
Fragments arranged by size:
4357 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2068 2965 2068
Size: 897 5005
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5902
PsiI TTA'TAA
Cuts at: 3472 3472
Size: 5902
PstI C_TGCA'G
Cuts at: 830 2379 3254 830
Size: 1549 875 3478
Fragments arranged by size:
3478 1549 875
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5902
PvuII CAG'CTG
Cuts at: 2860 2860
Size: 5902
RsrII CG'GwC_CG
Cuts at: 2280 2280
Size: 5902
SalI G'TCGA_C
Cuts at: 1936 3244 1936
Size: 1308 4594
SanDI GG'GwC_CC
Cuts at: 2068 2068
Size: 5902
SapI GCTCTTCn'nnn_
Cuts at: 2177 2612 2177
Size: 435 5467
SbfI CC_TGCA'GG
Cuts at: 3254 3254
Size: 5902
ScaI AGT'ACT
Cuts at: 4150 5862 4150
Size: 1712 4190
SfcI C'TryA_G
Cuts at: 826 1045 2375 3250 4385 5066 5257 826
Size: 219 1330 875 1135 681 191 1471
Fragments arranged by size:
1471 1330 1135 875 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5902
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5902
SmaI CCC'GGG
Cuts at: 1872 3229 1872
Size: 1357 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4006 4907 5169 820
Size: 197 926 2063 901 262 1553
Fragments arranged by size:
2063 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2878 3693 493
Size: 2385 815 2702
Fragments arranged by size:
2702 2385 815
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5902
SphI G_CATG'C
Cuts at: 1132 3260 1132
Size: 2128 3774
SspI AAT'ATT
Cuts at: 5 52 2444 3826 5
Size: 47 2392 1382 2081
Fragments arranged by size:
2392 2081 1382 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5902
StyI C'CwwG_G
Cuts at: 513 1065 2688 2995 3196 3341 513
Size: 552 1623 307 201 145 3074
Fragments arranged by size:
3074 1623 552 307 201 145
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3929 3946 4099 4284 4729 325
Size: 1255 2349 17 153 185 445 1498
Fragments arranged by size:
2349 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2406 4148 5860
Size: 274 80 33 51 1443 429 1742 1712
Cuts at: 5860 96
Size: 138
Fragments arranged by size:
1742 1712 1443 429 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5862 5894 5862
Size: 32 5870
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5902
XbaI T'CTAG_A
Cuts at: 3238 3238
Size: 5902
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2677 2980 2677
Size: 303 5599
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5902
XmaI C'CCGG_G
Cuts at: 1870 3227 1870
Size: 1357 4545
XmnI GAAnn'nnTTC
Cuts at: 4031 4031
Size: 5902
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3710 276
Size: 53 83 186 936 2176 2468
Fragments arranged by size:
2468 2176 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII RsrII SalI SanDI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI SacI
SacII SexAI SfiI SfoI SrfI SwaI