Restriction map for
pFN21AE9425
(Circular) (Six-base) MAPSORT of: pFN21AE9425.seq Check: 5183 from: 1 to: 8371
pFN21AE9425 8371 bp
With 182 enzymes: SgfI *
July 6, 2012 17:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6181 278
Size: 53 83 186 936 4645 2468
Fragments arranged by size:
4645 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3006 5714 1937
Size: 1069 2708 4594
Fragments arranged by size:
4594 2708 1069
Acc65I G'GTAC_C
Cuts at: 5692 5692
Size: 8371
AclI AA'CG_TT
Cuts at: 1563 6498 6871 1563
Size: 4935 373 3063
Fragments arranged by size:
4935 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3894 4286 5635 6434 7818 8086 1632
Size: 2262 392 1349 799 1384 268 1917
Fragments arranged by size:
2262 1917 1384 1349 799 392 268
AflII C'TTAA_G
Cuts at: 820 1017 2461 3835 820
Size: 197 1444 1374 5356
Fragments arranged by size:
5356 1444 1374 197
AflIII A'CryG_T
Cuts at: 1203 3931 4374 4782 7270 8247 1203
Size: 2728 443 408 2488 977 1327
Fragments arranged by size:
2728 2488 1327 977 443 408
AgeI A'CCGG_T
Cuts at: 2228 7202 2228
Size: 4974 3397
AhdI GACnn_n'nnGTC
Cuts at: 3135 7100 3135
Size: 3965 4406
AleI CACnn'nnGTG
Cuts at: 1166 3291 1166
Size: 2125 6246
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4658 1184
Size: 3474 4897
AlwNI CAG_nnn'CTG
Cuts at: 4264 6114 7686 4264
Size: 1850 1572 4949
Fragments arranged by size:
4949 1850 1572
ApaI G_GGCC'C
Cuts at: 3361 3361
Size: 8371
ApaLI G'TGCA_C
Cuts at: 6428 7584 6428
Size: 1156 7215
ApoI r'AATT_y
Cuts at: 1493 2330 2803 2944 3697 4003 4114 4838 4952
Size: 837 473 141 753 306 111 724 114
Cuts at: 4952 5680 5906 6058 8167 1493
Size: 728 226 152 2109 1697
Fragments arranged by size:
2109 1697 837 753 728 724 473 306
226 152 141 114 111
AseI AT'TA_AT
Cuts at: 160 1032 6925 160
Size: 872 5893 1606
Fragments arranged by size:
5893 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8371
AvaI C'yCGr_G
Cuts at: 1870 1943 5696 1870
Size: 73 3753 4545
Fragments arranged by size:
4545 3753 73
BamHI G'GATC_C
Cuts at: 4072 4270 5701 6068 4072
Size: 198 1431 367 6375
Fragments arranged by size:
6375 1431 367 198
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5692 7147 618
Size: 325 217 627 3905 1455 1842
Fragments arranged by size:
3905 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2224 2544 2792 2888 3361 5690 1391
Size: 833 320 248 96 473 2329 4072
Fragments arranged by size:
4072 2329 833 473 320 248 96
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2595 2739 4010 5397 928
Size: 971 696 144 1271 1387 3902
Fragments arranged by size:
3902 1387 1271 971 696 144
BbvCI CC'TCA_GC
Cuts at: 2978 3727 2978
Size: 749 7622
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2365 2399 6562 6596 1903
Size: 34 428 34 4163 34 3678
Fragments arranged by size:
4163 3678 428 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6263 7473 6263
Size: 1210 7161
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8371
BfrBI ATG'CAT
Cuts at: 2386 2624 2711 2386
Size: 238 87 8046
Fragments arranged by size:
8046 238 87
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6982 136
Size: 107 122 71 631 605 5310 1525
Fragments arranged by size:
5310 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5058 8366 5058
Size: 3308 5063
BlpI GC'TnA_GC
Cuts at: 5788 5788
Size: 8371
Bme1580I G_kGCm'C
Cuts at: 1790 3361 6432 7588 1790
Size: 1571 3071 1156 2573
Fragments arranged by size:
3071 2573 1571 1156
BmgBI CAC'GTC
Cuts at: 1318 4377 1318
Size: 3059 5312
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2049 3494 3643
Size: 416 53 83 399 24 632 1445 149
Cuts at: 3643 6102 7060 442
Size: 2459 958 1753
Fragments arranged by size:
2459 1753 1445 958 632 416 399 149
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8371
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3318 7031 1239
Size: 2079 3713 2579
Fragments arranged by size:
3713 2579 2079
Bpu10I CC'TnA_GC
Cuts at: 2978 3065 3727 4631 2978
Size: 87 662 904 6718
Fragments arranged by size:
6718 904 662 87
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2353 3350 3475 3881 4130 6496 7361 7659 2353
Size: 997 125 406 249 2366 865 298 3065
Fragments arranged by size:
3065 2366 997 865 406 298 249 125
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7034 882
Size: 617 5535 2219
Fragments arranged by size:
5535 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 6162 493
Size: 711 4958 2702
Fragments arranged by size:
4958 2702 711
BsaBI GATnn'nnATC
Cuts at: 5544 8365 5544
Size: 2821 5550
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6178 6560 7195 275
Size: 53 83 186 936 4645 382 635 1451
Fragments arranged by size:
4645 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2228 5088 6804 7202 7476 7623 8034 2228
Size: 2860 1716 398 274 147 411 2565
Fragments arranged by size:
2860 2565 1716 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5201 5231 1178
Size: 30 165 30 3798 30 4318
Fragments arranged by size:
4318 3798 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4649 1186
Size: 3463 4908
BseYI C'CCAG_C
Cuts at: 3094 4854 4997 7574 8070 3094
Size: 1760 143 2577 496 3395
Fragments arranged by size:
3395 2577 1760 496 143
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3138 4255 5075 1163
Size: 573 1402 1117 820 4459
Fragments arranged by size:
4459 1402 1117 820 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5821 6582 7610 1540
Size: 275 186 3820 761 1028 2301
Fragments arranged by size:
3820 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2544 4153 4637 6432 6517 7588 2544
Size: 1609 484 1795 85 1071 3327
Fragments arranged by size:
3327 1795 1609 1071 484 85
BsmI GAATG_Cn'
Cuts at: 2962 5171 5882 5975 2962
Size: 2209 711 93 5358
Fragments arranged by size:
5358 2209 711 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2224 2544 2792 2888 3361 4153 4637
Size: 399 434 320 248 96 473 792 484
Cuts at: 4637 5690 6432 6517 7588 1391
Size: 1053 742 85 1071 2174
Fragments arranged by size:
2174 1071 1053 792 742 484 473 434
399 320 248 96 85
BspEI T'CCGG_A
Cuts at: 8034 8034
Size: 8371
BspHI T'CATG_A
Cuts at: 6260 7990 6260
Size: 1730 6641
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5127 5726 844
Size: 4283 599 3489
Fragments arranged by size:
4283 3489 599
BsrBI CCG'CTC
Cuts at: 1394 1823 3091 6258 1394
Size: 429 1268 3167 3507
Fragments arranged by size:
3507 3167 1268 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4710 4788 5028 5608 6866 7040 65
Size: 1183 3462 78 240 580 1258 174 1396
Fragments arranged by size:
3462 1396 1258 1183 580 240 174 78
BsrFI r'CCGG_y
Cuts at: 1700 1810 2228 7015 7202 1700
Size: 110 418 4787 187 2869
Fragments arranged by size:
4787 2869 418 187 110
BsrGI T'GTAC_A
Cuts at: 96 4785 96
Size: 4689 3682
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8371
BssSI C'ACGA_G
Cuts at: 6431 7443 6431
Size: 1012 7359
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5177 794
Size: 4383 3988
BstBI TT'CG_AA
Cuts at: 7175 7175
Size: 8371
BstEII G'GTnAC_C
Cuts at: 1184 3625 1184
Size: 2441 5930
BstXI CCAn_nnnn'nTGG
Cuts at: 3622 3748 5540 3622
Size: 126 1792 6453
Fragments arranged by size:
6453 1792 126
BstYI r'GATC_y
Cuts at: 1972 4072 4270 4339 4969 5058 5701 6068 6453
Size: 2100 198 69 630 89 643 367 385
Cuts at: 6453 6470 7922 8008 8020 8366 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
2100 1977 1452 643 630 385 367 346
198 89 86 69 17 12
Bsu36I CC'TnA_GG
Cuts at: 3195 3195
Size: 8371
BtgI C'CryG_G
Cuts at: 513 1065 3501 5505 513
Size: 552 2436 2004 3379
Fragments arranged by size:
3379 2436 2004 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7632
Fragments arranged by size:
7632 683 56
BtsI GCAGTG_nn'
Cuts at: 5131 5887 6681 6701 5131
Size: 756 794 20 6801
Fragments arranged by size:
6801 794 756 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8371
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8336
DraI TTT'AAA
Cuts at: 3663 3696 3882 5675 3663
Size: 33 186 1793 6359
Fragments arranged by size:
6359 1793 186 33
DraIII CAC_nnn'GTG
Cuts at: 2107 2107
Size: 8371
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3486 7378 809
Size: 507 2170 3892 1802
Fragments arranged by size:
3892 2170 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2581 3108 3527
Size: 54 1425 50 275 18 751 527 419
Cuts at: 3527 5818 6707 8336 8
Size: 2291 889 1629 43
Fragments arranged by size:
2291 1629 1425 889 751 527 419 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5818 1537
Size: 275 4006 4090
Fragments arranged by size:
4090 4006 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4141 4667 5219 6301 1353
Size: 2788 526 552 1082 3423
Fragments arranged by size:
3423 2788 1082 552 526
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6958 7332 7478 387
Size: 1064 138 5369 374 146 1280
Fragments arranged by size:
5369 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2542 2542
Size: 8371
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3318 3894 4286 5635 6434 7031 7818
Size: 393 1686 576 392 1349 799 597 787
Cuts at: 7818 8086 1239
Size: 268 1524
Fragments arranged by size:
1686 1524 1349 799 787 597 576 393
392 268
EcoO109I rG'GnC_Cy
Cuts at: 2154 3129 3358 3638 4196 5186 2154
Size: 975 229 280 558 990 5339
Fragments arranged by size:
5339 990 975 558 280 229
EcoRI G'AATT_C
Cuts at: 4952 5680 6058 4952
Size: 728 378 7265
Fragments arranged by size:
7265 728 378
EcoRV GAT'ATC
Cuts at: 3649 3649
Size: 8371
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3595 4227 3595
Size: 632 7739
FspI TGC'GCA
Cuts at: 5123 6877 5123
Size: 1754 6617
HaeII r_GCGC'y
Cuts at: 1678 7518 1678
Size: 5840 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2285 2317 3121 3153 4595
Size: 32 135 32 713 32 804 32 1442
Cuts at: 4595 4627 4954 4986 5537 5569 7012 7044 7086
Size: 32 327 32 551 32 1443 32 42
Cuts at: 7086 7118 1373
Size: 32 2626
Fragments arranged by size:
2626 1443 1442 804 713 551 327 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3007 5715 5961 669
Size: 988 281 1069 2708 246 3079
Fragments arranged by size:
3079 2708 1069 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2749 3876 748
Size: 2001 1127 5243
Fragments arranged by size:
5243 2001 1127
HpaI GTT'AAC
Cuts at: 5961 5961
Size: 8371
KpnI G_GTAC'C
Cuts at: 5696 5696
Size: 8371
MfeI C'AATT_G
Cuts at: 2281 2636 3888 5970 2281
Size: 355 1252 2082 4682
Fragments arranged by size:
4682 2082 1252 355
MluI A'CGCG_T
Cuts at: 8247 8247
Size: 8371
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2166 2997 3562 3678 4940 5448 7485 7669
Size: 861 831 565 116 1262 508 2037 184
Cuts at: 7669 1305
Size: 2007
Fragments arranged by size:
2037 2007 1262 861 831 565 508 184
116
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2583 3110 3529 10
Size: 54 1425 343 751 527 419 4852
Fragments arranged by size:
4852 1425 751 527 419 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3291 5033 5538 6329 6688 6847 518
Size: 648 2125 1742 505 791 359 159 2042
Fragments arranged by size:
2125 2042 1742 791 648 505 359 159
MspA1I CmG'CkG
Cuts at: 1606 2982 3114 5787 6464 7612 7857 1606
Size: 1376 132 2673 677 1148 245 2120
Fragments arranged by size:
2673 2120 1376 1148 677 245 132
NcoI C'CATG_G
Cuts at: 513 1065 3501 5505 513
Size: 552 2436 2004 3379
Fragments arranged by size:
3379 2436 2004 552
NdeI CA'TA_TG
Cuts at: 387 4576 387
Size: 4189 4182
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8371
NotI GC'GGCC_GC
Cuts at: 5818 5818
Size: 8371
NsiI A_TGCA'T
Cuts at: 2388 2626 2713 2388
Size: 238 87 8046
Fragments arranged by size:
8046 238 87
NspI r_CATG'y
Cuts at: 1132 1229 2209 2390 3935 4786 5729 7274 1132
Size: 97 980 181 1545 851 943 1545 2229
Fragments arranged by size:
2229 1545 1545 980 943 851 181 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8371
PciI A'CATG_T
Cuts at: 3931 4782 7270 3931
Size: 851 2488 5032
Fragments arranged by size:
5032 2488 851
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8371
PfoI T'CCnGG_A
Cuts at: 4185 4185
Size: 8371
PmeI GTTT'AAAC
Cuts at: 5675 5675
Size: 8371
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3710 3742 6440 6472 7953 7985 3710
Size: 32 2698 32 1481 32 4096
Fragments arranged by size:
4096 2698 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2154 3129 3638 4196 5186 2154
Size: 975 509 558 990 5339
Fragments arranged by size:
5339 990 975 558 509
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8371
PsiI TTA'TAA
Cuts at: 2928 5941 2928
Size: 3013 5358
PspOMI G'GGCC_C
Cuts at: 3357 3357
Size: 8371
PstI C_TGCA'G
Cuts at: 830 5723 830
Size: 4893 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8371
PvuII CAG'CTG
Cuts at: 2982 3114 2982
Size: 132 8239
SacI G_AGCT'C
Cuts at: 2544 2544
Size: 8371
SalI G'TCGA_C
Cuts at: 1936 3005 5713 1936
Size: 1069 2708 4594
Fragments arranged by size:
4594 2708 1069
SanDI GG'GwC_CC
Cuts at: 3129 5186 3129
Size: 2057 6314
SapI GCTCTTCn'nnn_
Cuts at: 4141 4141
Size: 8371
SbfI CC_TGCA'GG
Cuts at: 5723 5723
Size: 8371
ScaI AGT'ACT
Cuts at: 6619 8331 6619
Size: 1712 6659
SexAI A'CCwGG_T
Cuts at: 2505 2505
Size: 8371
SfcI C'TryA_G
Cuts at: 826 1045 3154 5719 6854 7535 7726 826
Size: 219 2109 2565 1135 681 191 1471
Fragments arranged by size:
2565 2109 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8371
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8371
SmaI CCC'GGG
Cuts at: 1872 5698 1872
Size: 3826 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2368 2461 3329 3454 3835 3860
Size: 197 926 425 93 868 125 381 25
Cuts at: 3860 4109 6475 7376 7638 820
Size: 249 2366 901 262 1553
Fragments arranged by size:
2366 1553 926 901 868 425 381 262
249 197 125 93 25
SnaBI TAC'GTA
Cuts at: 493 6162 493
Size: 5669 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8371
SphI G_CATG'C
Cuts at: 1132 5729 1132
Size: 4597 3774
SspI AAT'ATT
Cuts at: 5 52 3736 3940 5632 6295 5
Size: 47 3684 204 1692 663 2081
Fragments arranged by size:
3684 2081 1692 663 204 47
StuI AGG'CCT
Cuts at: 1517 2355 1517
Size: 838 7533
StyI C'CwwG_G
Cuts at: 513 1065 3341 3441 3501 5505 5810 513
Size: 552 2276 100 60 2004 305 3074
Fragments arranged by size:
3074 2276 2004 552 305 100 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4521 6398 6415 6568 6753 7198 325
Size: 1255 2941 1877 17 153 185 445 1498
Fragments arranged by size:
2941 1877 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3205 4785 6617
Size: 274 80 33 51 1443 1228 1580 1832
Cuts at: 6617 8329 96
Size: 1712 138
Fragments arranged by size:
1832 1712 1580 1443 1228 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8331 8363 8331
Size: 32 8339
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8371
XbaI T'CTAG_A
Cuts at: 5707 5707
Size: 8371
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2128 5041 2128
Size: 2913 5458
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8371
XmaI C'CCGG_G
Cuts at: 1870 5696 1870
Size: 3826 4545
XmnI GAAnn'nnTTC
Cuts at: 2426 2826 3552 4776 6500 2426
Size: 400 726 1224 1724 4297
Fragments arranged by size:
4297 1724 1224 726 400
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6179 276
Size: 53 83 186 936 4645 2468
Fragments arranged by size:
4645 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstZ17I EcoNI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII
SacII SfiI SfoI SrfI SwaI