Restriction map for
pFN21AE9420
(Circular) (Six-base) MAPSORT of: pFN21AE9420.seq Check: 5673 from: 1 to: 5716
pFN21AE9420 5716 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3526 278
Size: 53 83 186 936 1990 2468
Fragments arranged by size:
2468 1990 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2826 3059 1937
Size: 889 233 4594
Fragments arranged by size:
4594 889 233
Acc65I G'GTAC_C
Cuts at: 3037 3037
Size: 5716
AclI AA'CG_TT
Cuts at: 1563 3843 4216 1563
Size: 2280 373 3063
Fragments arranged by size:
3063 2280 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2052 3779 5163 5431 1632
Size: 420 1727 1384 268 1917
Fragments arranged by size:
1917 1727 1384 420 268
AfeI AGC'GCT
Cuts at: 2196 2196
Size: 5716
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5519
AflIII A'CryG_T
Cuts at: 1203 2244 4615 5592 1203
Size: 1041 2371 977 1327
Fragments arranged by size:
2371 1327 1041 977
AgeI A'CCGG_T
Cuts at: 4547 4547
Size: 5716
AhdI GACnn_n'nnGTC
Cuts at: 4445 4445
Size: 5716
AleI CACnn'nnGTG
Cuts at: 1166 2522 1166
Size: 1356 4360
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5716
AlwNI CAG_nnn'CTG
Cuts at: 2551 2587 3459 5031 2551
Size: 36 872 1572 3236
Fragments arranged by size:
3236 1572 872 36
ApaLI G'TGCA_C
Cuts at: 3773 4929 3773
Size: 1156 4560
ApoI r'AATT_y
Cuts at: 1493 2597 3025 3251 3403 5512 1493
Size: 1104 428 226 152 2109 1697
Fragments arranged by size:
2109 1697 1104 428 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4270 160
Size: 872 3238 1606
Fragments arranged by size:
3238 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5716
AvaI C'yCGr_G
Cuts at: 1870 1943 3041 1870
Size: 73 1098 4545
Fragments arranged by size:
4545 1098 73
BamHI G'GATC_C
Cuts at: 3046 3413 3046
Size: 367 5349
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2485 3037 4492 618
Size: 325 217 627 698 552 1455 1842
Fragments arranged by size:
1842 1455 698 627 552 325 217
BanII G_rGCy'C
Cuts at: 1391 3035 1391
Size: 1644 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2008 2968 928
Size: 971 109 960 3676
Fragments arranged by size:
3676 971 960 109
BbvCI CC'TCA_GC
Cuts at: 2273 2273
Size: 5716
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2484 2518 3907 3941 1903
Size: 34 547 34 1389 34 3678
Fragments arranged by size:
3678 1389 547 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3608 4818 3608
Size: 1210 4506
BclI T'GATC_A
Cuts at: 1548 2649 1548
Size: 1101 4615
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4327 136
Size: 107 122 71 631 605 2655 1525
Fragments arranged by size:
2655 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2667 5711 2667
Size: 3044 2672
BlpI GC'TnA_GC
Cuts at: 2540 2694 3133 2540
Size: 154 439 5123
Fragments arranged by size:
5123 439 154
Bme1580I G_kGCm'C
Cuts at: 1790 2490 3777 4933 1790
Size: 700 1287 1156 2573
Fragments arranged by size:
2573 1287 1156 700
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5716
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3447 4405 442
Size: 416 53 83 399 24 2030 958 1753
Fragments arranged by size:
2030 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5716
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2472 2472
Size: 5716
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4376 1239
Size: 3137 2579
Bpu10I CC'TnA_GC
Cuts at: 2273 2273
Size: 5716
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3841 4706 5004 3841
Size: 865 298 4553
Fragments arranged by size:
4553 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4379 882
Size: 617 2880 2219
Fragments arranged by size:
2880 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3507 493
Size: 711 2303 2702
Fragments arranged by size:
2702 2303 711
BsaBI GATnn'nnATC
Cuts at: 5710 5710
Size: 5716
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3523 3905 4540 275
Size: 53 83 186 936 1990 382 635 1451
Fragments arranged by size:
1990 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4149 4547 4821 4968 5379 4149
Size: 398 274 147 411 4486
Fragments arranged by size:
4486 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5491
Fragments arranged by size:
5491 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5716
BseYI C'CCAG_C
Cuts at: 2726 4919 5415 2726
Size: 2193 496 3027
Fragments arranged by size:
3027 2193 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2148 2315 1163
Size: 573 412 167 4564
Fragments arranged by size:
4564 573 412 167
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3166 3927 4955 1540
Size: 275 186 1165 761 1028 2301
Fragments arranged by size:
2301 1165 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2040 3777 3862 4933 2040
Size: 1737 85 1071 2823
Fragments arranged by size:
2823 1737 1071 85
BsmI GAATG_Cn'
Cuts at: 2048 3227 3320 2048
Size: 1179 93 4444
Fragments arranged by size:
4444 1179 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2490 3035 3777 3862 4933 1391
Size: 399 250 450 545 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 545 450 399 250 85
BspEI T'CCGG_A
Cuts at: 5379 5379
Size: 5716
BspHI T'CATG_A
Cuts at: 3605 5335 3605
Size: 1730 3986
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3071 844
Size: 2227 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3603 1394
Size: 429 1780 3507
Fragments arranged by size:
3507 1780 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2769 4211 4385 65
Size: 1183 1521 1442 174 1396
Fragments arranged by size:
1521 1442 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4360 4547 1700
Size: 110 2550 187 2869
Fragments arranged by size:
2869 2550 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5716
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5716
BssSI C'ACGA_G
Cuts at: 3776 4788 3776
Size: 1012 4704
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5716
BstBI TT'CG_AA
Cuts at: 4520 4520
Size: 5716
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5716
BstXI CCAn_nnnn'nTGG
Cuts at: 2066 2764 2066
Size: 698 5018
BstYI r'GATC_y
Cuts at: 1972 2667 3046 3413 3798 3815 5267 5353 5365
Size: 695 379 367 385 17 1452 86 12
Cuts at: 5365 5711 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 695 385 379 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5164
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4977
Fragments arranged by size:
4977 683 56
BtsI GCAGTG_nn'
Cuts at: 3232 4026 4046 3232
Size: 794 20 4902
Fragments arranged by size:
4902 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5716
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5681
DraI TTT'AAA
Cuts at: 3020 3020
Size: 5716
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2822 4723 809
Size: 507 1506 1901 1802
Fragments arranged by size:
1901 1802 1506 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3163 4052 5681
Size: 54 1425 50 275 18 1333 889 1629
Cuts at: 5681 8
Size: 43
Fragments arranged by size:
1629 1425 1333 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3163 1537
Size: 275 1351 4090
Fragments arranged by size:
4090 1351 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2028 2357 2550 3646 1353
Size: 675 329 193 1096 3423
Fragments arranged by size:
3423 1096 675 329 193
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4303 4677 4823 387
Size: 1064 138 2714 374 146 1280
Fragments arranged by size:
2714 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2052 3779 4376 5163 5431 1239
Size: 393 420 1727 597 787 268 1524
Fragments arranged by size:
1727 1524 787 597 420 393 268
EcoRI G'AATT_C
Cuts at: 3025 3403 3025
Size: 378 5338
FspI TGC'GCA
Cuts at: 4222 4222
Size: 5716
HaeII r_GCGC'y
Cuts at: 1678 2198 4863 1678
Size: 520 2665 2531
Fragments arranged by size:
2665 2531 520
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4357 4389 4431 4463 1373
Size: 32 135 32 2785 32 42 32 2626
Fragments arranged by size:
2785 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2827 3060 3306 669
Size: 988 281 889 233 246 3079
Fragments arranged by size:
3079 988 889 281 246 233
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5716
HpaI GTT'AAC
Cuts at: 3306 3306
Size: 5716
KpnI G_GTAC'C
Cuts at: 3041 3041
Size: 5716
MfeI C'AATT_G
Cuts at: 3315 3315
Size: 5716
MluI A'CGCG_T
Cuts at: 5592 5592
Size: 5716
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4830 5014 1305
Size: 3525 184 2007
Fragments arranged by size:
3525 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3894
Fragments arranged by size:
3894 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2522 2774 3674 4033 4192 518
Size: 648 1356 252 900 359 159 2042
Fragments arranged by size:
2042 1356 900 648 359 252 159
MspA1I CmG'CkG
Cuts at: 1606 2584 3132 3809 4957 5202 1606
Size: 978 548 677 1148 245 2120
Fragments arranged by size:
2120 1148 978 677 548 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5164
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5716
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5716
NotI GC'GGCC_GC
Cuts at: 3163 3163
Size: 5716
NspI r_CATG'y
Cuts at: 1132 1229 2140 2248 3074 4619 1132
Size: 97 911 108 826 1545 2229
Fragments arranged by size:
2229 1545 911 826 108 97
PasI CC'CwG_GG
Cuts at: 1348 2764 1348
Size: 1416 4300
PciI A'CATG_T
Cuts at: 2244 4615 2244
Size: 2371 3345
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2551 1108
Size: 1443 4273
PmeI GTTT'AAAC
Cuts at: 3020 3020
Size: 5716
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3785 3817 5298 5330 3785
Size: 32 1481 32 4171
Fragments arranged by size:
4171 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5716
PsiI TTA'TAA
Cuts at: 3286 3286
Size: 5716
PstI C_TGCA'G
Cuts at: 830 3068 830
Size: 2238 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5716
PvuII CAG'CTG
Cuts at: 2584 2584
Size: 5716
SalI G'TCGA_C
Cuts at: 1936 2825 3058 1936
Size: 889 233 4594
Fragments arranged by size:
4594 889 233
SapI GCTCTTCn'nnn_
Cuts at: 2028 2028
Size: 5716
SbfI CC_TGCA'GG
Cuts at: 3068 3068
Size: 5716
ScaI AGT'ACT
Cuts at: 3964 5676 3964
Size: 1712 4004
SexAI A'CCwGG_T
Cuts at: 2058 2058
Size: 5716
SfcI C'TryA_G
Cuts at: 826 1045 3064 4199 4880 5071 826
Size: 219 2019 1135 681 191 1471
Fragments arranged by size:
2019 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5716
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5716
SmaI CCC'GGG
Cuts at: 1872 3043 1872
Size: 1171 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3820 4721 4983 820
Size: 197 926 1877 901 262 1553
Fragments arranged by size:
1877 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3507 493
Size: 3014 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5716
SphI G_CATG'C
Cuts at: 1132 2140 3074 1132
Size: 1008 934 3774
Fragments arranged by size:
3774 1008 934
SspI AAT'ATT
Cuts at: 5 52 3640 5
Size: 47 3588 2081
Fragments arranged by size:
3588 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5716
StyI C'CwwG_G
Cuts at: 513 1065 2023 3155 513
Size: 552 958 1132 3074
Fragments arranged by size:
3074 1132 958 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3743 3760 3913 4098 4543 325
Size: 1255 2163 17 153 185 445 1498
Fragments arranged by size:
2163 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2086 3962 5674
Size: 274 80 33 51 1443 109 1876 1712
Cuts at: 5674 96
Size: 138
Fragments arranged by size:
1876 1712 1443 274 138 109 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2469 2501 5676 5708 2469
Size: 32 3175 32 2477
Fragments arranged by size:
3175 2477 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5716
XbaI T'CTAG_A
Cuts at: 3052 3052
Size: 5716
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2287 2818 2287
Size: 531 5185
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5716
XmaI C'CCGG_G
Cuts at: 1870 3041 1870
Size: 1171 4545
XmnI GAAnn'nnTTC
Cuts at: 2975 3845 2975
Size: 870 4846
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3524 276
Size: 53 83 186 936 1990 2468
Fragments arranged by size:
2468 1990 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI
SwaI