Restriction map for
pFN21AE9413
(Circular) (Six-base) MAPSORT of: pFN21AE9413.seq Check: 5262 from: 1 to: 5821
pFN21AE9413 5821 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2933 2933
Size: 5821
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3631 278
Size: 53 83 186 936 2095 2468
Fragments arranged by size:
2468 2095 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2859 2973 3090 3164 1937
Size: 922 114 117 74 4594
Fragments arranged by size:
4594 922 117 114 74
Acc65I G'GTAC_C
Cuts at: 3142 3142
Size: 5821
AclI AA'CG_TT
Cuts at: 1563 3948 4321 1563
Size: 2385 373 3063
Fragments arranged by size:
3063 2385 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3884 5268 5536 1632
Size: 2252 1384 268 1917
Fragments arranged by size:
2252 1917 1384 268
AfeI AGC'GCT
Cuts at: 2888 2888
Size: 5821
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5624
AflIII A'CryG_T
Cuts at: 1203 2649 3093 4720 5697 1203
Size: 1446 444 1627 977 1327
Fragments arranged by size:
1627 1446 1327 977 444
AgeI A'CCGG_T
Cuts at: 4652 4652
Size: 5821
AhdI GACnn_n'nnGTC
Cuts at: 2077 4550 2077
Size: 2473 3348
AleI CACnn'nnGTG
Cuts at: 1166 2600 1166
Size: 1434 4387
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3032 1184
Size: 1848 3973
AlwNI CAG_nnn'CTG
Cuts at: 2925 3564 5136 2925
Size: 639 1572 3610
Fragments arranged by size:
3610 1572 639
ApaI G_GGCC'C
Cuts at: 2499 2542 2499
Size: 43 5778
ApaLI G'TGCA_C
Cuts at: 2690 3878 5034 2690
Size: 1188 1156 3477
Fragments arranged by size:
3477 1188 1156
ApoI r'AATT_y
Cuts at: 1493 2993 3130 3356 3508 5617 1493
Size: 1500 137 226 152 2109 1697
Fragments arranged by size:
2109 1697 1500 226 152 137
AseI AT'TA_AT
Cuts at: 160 1032 4375 160
Size: 872 3343 1606
Fragments arranged by size:
3343 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
AvaI C'yCGr_G
Cuts at: 1870 1943 2074 3146 1870
Size: 73 131 1072 4545
Fragments arranged by size:
4545 1072 131 73
BamHI G'GATC_C
Cuts at: 3060 3151 3518 3060
Size: 91 367 5363
Fragments arranged by size:
5363 367 91
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2527 2745 3142 4597 618
Size: 325 217 627 740 218 397 1455 1842
Fragments arranged by size:
1842 1455 740 627 397 325 218 217
BanII G_rGCy'C
Cuts at: 1391 2499 2542 2571 3140 1391
Size: 1108 43 29 569 4072
Fragments arranged by size:
4072 1108 569 43 29
BbeI G_GCGC'C
Cuts at: 2749 2749
Size: 5821
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2950 928
Size: 971 1051 3799
Fragments arranged by size:
3799 1051 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4012 4046 1903
Size: 34 2075 34 3678
Fragments arranged by size:
3678 2075 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3713 4923 3713
Size: 1210 4611
BclI T'GATC_A
Cuts at: 1548 2454 1548
Size: 906 4915
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2231 4432 136
Size: 107 122 71 631 605 559 2201 1525
Fragments arranged by size:
2201 1525 631 605 559 122 107 71
BglII A'GATC_T
Cuts at: 5816 5816
Size: 5821
BlpI GC'TnA_GC
Cuts at: 3238 3238
Size: 5821
Bme1580I G_kGCm'C
Cuts at: 1790 2499 2542 2694 3882 5038 1790
Size: 709 43 152 1188 1156 2573
Fragments arranged by size:
2573 1188 1156 709 152 43
BmgBI CAC'GTC
Cuts at: 1318 2022 1318
Size: 704 5117
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2080 3552 4510
Size: 416 53 83 399 24 663 1472 958
Cuts at: 4510 442
Size: 1753
Fragments arranged by size:
1753 1472 958 663 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5821
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2381 3001 4481 1239
Size: 1142 620 1480 2579
Fragments arranged by size:
2579 1480 1142 620
Bpu10I CC'TnA_GC
Cuts at: 3054 3054
Size: 5821
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2048 2800 2933 3946 4811 5109 2048
Size: 752 133 1013 865 298 2760
Fragments arranged by size:
2760 1013 865 752 298 133
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2470 4484 882
Size: 617 971 2014 2219
Fragments arranged by size:
2219 2014 971 617
BsaAI yAC'GTr
Cuts at: 493 1204 2590 3094 3612 493
Size: 711 1386 504 518 2702
Fragments arranged by size:
2702 1386 711 518 504
BsaBI GATnn'nnATC
Cuts at: 5815 5815
Size: 5821
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2631 2746 3628 4010
Size: 53 83 186 936 1098 115 882 382
Cuts at: 4010 4645 275
Size: 635 1451
Fragments arranged by size:
1451 1098 936 882 635 382 186 115
83 53
BsaWI w'CCGG_w
Cuts at: 4254 4652 4926 5073 5484 4254
Size: 398 274 147 411 4591
Fragments arranged by size:
4591 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5596
Fragments arranged by size:
5596 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5821
BseYI C'CCAG_C
Cuts at: 2554 5024 5520 2554
Size: 2470 496 2855
Fragments arranged by size:
2855 2470 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5248
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3271 4032 5060 1540
Size: 275 186 1270 761 1028 2301
Fragments arranged by size:
2301 1270 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2571 2694 3882 3967 5038 2571
Size: 123 1188 85 1071 3354
Fragments arranged by size:
3354 1188 1071 123 85
BsmI GAATG_Cn'
Cuts at: 3332 3425 3332
Size: 93 5728
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2499 2542 2571 2694 3140 3882 3967
Size: 399 709 43 29 123 446 742 85
Cuts at: 3967 5038 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 709 446 399 123 85
43 29
BspEI T'CCGG_A
Cuts at: 5484 5484
Size: 5821
BspHI T'CATG_A
Cuts at: 3710 5440 3710
Size: 1730 4091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2052 2223 2724 2933 3176 844
Size: 1208 171 501 209 243 3489
Fragments arranged by size:
3489 1208 501 243 209 171
BsrBI CCG'CTC
Cuts at: 1394 1823 3058 3708 1394
Size: 429 1235 650 3507
Fragments arranged by size:
3507 1235 650 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3106 4316 4490 65
Size: 1183 1858 1210 174 1396
Fragments arranged by size:
1858 1396 1210 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2191 2530 4465 4652 1700
Size: 110 381 339 1935 187 2869
Fragments arranged by size:
2869 1935 381 339 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5821
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5821
BssSI C'ACGA_G
Cuts at: 3881 4893 3881
Size: 1012 4809
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5821
BstBI TT'CG_AA
Cuts at: 4625 4625
Size: 5821
BstEII G'GTnAC_C
Cuts at: 1184 2593 1184
Size: 1409 4412
BstYI r'GATC_y
Cuts at: 1972 2106 3060 3151 3518 3903 3920 5372 5458
Size: 134 954 91 367 385 17 1452 86
Cuts at: 5458 5470 5816 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 954 385 367 346 134 91
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2102 2102
Size: 5821
BtgI C'CryG_G
Cuts at: 513 1065 2004 2139 513
Size: 552 939 135 4195
Fragments arranged by size:
4195 939 552 135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2443 2473 487
Size: 683 56 1217 30 3835
Fragments arranged by size:
3835 1217 683 56 30
BtsI GCAGTG_nn'
Cuts at: 3337 4131 4151 3337
Size: 794 20 5007
Fragments arranged by size:
5007 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5821
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5786
DraI TTT'AAA
Cuts at: 3125 3125
Size: 5821
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4828 809
Size: 507 3512 1802
Fragments arranged by size:
3512 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2115 2205 2223
Size: 54 1425 50 275 18 285 90 18
Cuts at: 2223 2370 2607 3268 4157 5786 8
Size: 147 237 661 889 1629 43
Fragments arranged by size:
1629 1425 889 661 285 275 237 147
90 54 50 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 3268 1537
Size: 275 1456 4090
Fragments arranged by size:
4090 1456 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2825 3751 1353
Size: 1472 926 3423
Fragments arranged by size:
3423 1472 926
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4408 4782 4928 387
Size: 1064 138 2819 374 146 1280
Fragments arranged by size:
2819 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2569 2569
Size: 5821
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2381 3001 3884 4481 5268 5536 1239
Size: 393 749 620 883 597 787 268 1524
Fragments arranged by size:
1524 883 787 749 620 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2495 2496 2539 2495
Size: 1 43 5777
Fragments arranged by size:
5777 43 1
EcoRI G'AATT_C
Cuts at: 3130 3508 3130
Size: 378 5443
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2974 2974
Size: 5821
FspI TGC'GCA
Cuts at: 4327 4327
Size: 5821
HaeII r_GCGC'y
Cuts at: 1678 2422 2749 2890 4968 1678
Size: 744 327 141 2078 2531
Fragments arranged by size:
2531 2078 744 327 141
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2063 2095 4462 4494 4536
Size: 32 135 32 491 32 2367 32 42
Cuts at: 4536 4568 1373
Size: 32 2626
Fragments arranged by size:
2626 2367 491 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3165 3411 669
Size: 988 281 1227 246 3079
Fragments arranged by size:
3079 1227 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5821
HpaI GTT'AAC
Cuts at: 3411 3411
Size: 5821
KasI G'GCGC_C
Cuts at: 2745 2745
Size: 5821
KpnI G_GTAC'C
Cuts at: 3146 3146
Size: 5821
MfeI C'AATT_G
Cuts at: 3420 3420
Size: 5821
MluI A'CGCG_T
Cuts at: 5697 5697
Size: 5821
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2265 2682 4935 5119 1305
Size: 960 417 2253 184 2007
Fragments arranged by size:
2253 2007 960 417 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2117 2225 2372 10
Size: 54 1425 343 285 108 147 3459
Fragments arranged by size:
3459 1425 343 285 147 108 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2123 2177 2294 2390 2600 3779 4138
Size: 648 957 54 117 96 210 1179 359
Cuts at: 4138 4297 518
Size: 159 2042
Fragments arranged by size:
2042 1179 957 648 359 210 159 117
96 54
MspA1I CmG'CkG
Cuts at: 1606 2576 2755 3237 3914 5062 5307 1606
Size: 970 179 482 677 1148 245 2120
Fragments arranged by size:
2120 1148 970 677 482 245 179
NaeI GCC'GGC
Cuts at: 2193 2532 2193
Size: 339 5482
NarI GG'CG_CC
Cuts at: 2746 2746
Size: 5821
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4330
Fragments arranged by size:
4330 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5821
NgoMIV G'CCGG_C
Cuts at: 2191 2530 2191
Size: 339 5482
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5821
NotI GC'GGCC_GC
Cuts at: 3268 3268
Size: 5821
NspI r_CATG'y
Cuts at: 1132 1229 2269 2653 3179 4724 1132
Size: 97 1040 384 526 1545 2229
Fragments arranged by size:
2229 1545 1040 526 384 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5821
PciI A'CATG_T
Cuts at: 2649 4720 2649
Size: 2071 3750
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5821
PfoI T'CCnGG_A
Cuts at: 2358 2358
Size: 5821
PmeI GTTT'AAAC
Cuts at: 3125 3125
Size: 5821
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3890 3922 5403 5435 3890
Size: 32 1481 32 4276
Fragments arranged by size:
4276 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5821
PsiI TTA'TAA
Cuts at: 3391 3391
Size: 5821
PspOMI G'GGCC_C
Cuts at: 2495 2538 2495
Size: 43 5778
PstI C_TGCA'G
Cuts at: 830 2754 3173 830
Size: 1924 419 3478
Fragments arranged by size:
3478 1924 419
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5821
RsrII CG'GwC_CG
Cuts at: 2668 2668
Size: 5821
SacI G_AGCT'C
Cuts at: 2571 2571
Size: 5821
SalI G'TCGA_C
Cuts at: 1936 3163 1936
Size: 1227 4594
SbfI CC_TGCA'GG
Cuts at: 3173 3173
Size: 5821
ScaI AGT'ACT
Cuts at: 4069 5781 4069
Size: 1712 4109
SfcI C'TryA_G
Cuts at: 826 1045 2750 2860 3169 4304 4985 5176 826
Size: 219 1705 110 309 1135 681 191 1471
Fragments arranged by size:
1705 1471 1135 681 309 219 191 110
SfiI GGCCn_nnn'nGGCC
Cuts at: 2231 2231
Size: 5821
SfoI GGC'GCC
Cuts at: 2747 2747
Size: 5821
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5821
SmaI CCC'GGG
Cuts at: 1872 3148 1872
Size: 1276 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2027 2779 2948 3925 4826 5088
Size: 197 926 84 752 169 977 901 262
Cuts at: 5088 820
Size: 1553
Fragments arranged by size:
1553 977 926 901 752 262 197 169
84
SnaBI TAC'GTA
Cuts at: 493 3612 493
Size: 3119 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5821
SphI G_CATG'C
Cuts at: 1132 2269 3179 1132
Size: 1137 910 3774
Fragments arranged by size:
3774 1137 910
SspI AAT'ATT
Cuts at: 5 52 3745 5
Size: 47 3693 2081
Fragments arranged by size:
3693 2081 47
StuI AGG'CCT
Cuts at: 1517 2798 3005 1517
Size: 1281 207 4333
Fragments arranged by size:
4333 1281 207
StyI C'CwwG_G
Cuts at: 513 1065 2004 2112 2448 2598 2793 3260 513
Size: 552 939 108 336 150 195 467 3074
Fragments arranged by size:
3074 939 552 467 336 195 150 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2099 2461 3848 3865 4018 4203 4648
Size: 1255 519 362 1387 17 153 185 445
Cuts at: 4648 325
Size: 1498
Fragments arranged by size:
1498 1387 1255 519 445 362 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4067 5779 96
Size: 274 80 33 51 1443 2090 1712 138
Fragments arranged by size:
2090 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5781 5813 5781
Size: 32 5789
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5821
XbaI T'CTAG_A
Cuts at: 3157 3157
Size: 5821
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5821
XmaI C'CCGG_G
Cuts at: 1870 3146 1870
Size: 1276 4545
XmnI GAAnn'nnTTC
Cuts at: 3950 3950
Size: 5821
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3629 276
Size: 53 83 186 936 2095 2468
Fragments arranged by size:
2468 2095 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI RsrII SacI SalI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FseI FspAI
NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII
SacII SanDI SapI SexAI SrfI SwaI XcmI