Restriction map for
pFN21AE9412
(Circular) (Six-base) MAPSORT of: pFN21AE9412.seq Check: 7850 from: 1 to: 5632
pFN21AE9412 5632 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3442 278
Size: 53 83 186 936 1906 2468
Fragments arranged by size:
2468 1906 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2975 1937
Size: 1038 4594
Acc65I G'GTAC_C
Cuts at: 2953 2953
Size: 5632
AclI AA'CG_TT
Cuts at: 1563 3759 4132 1563
Size: 2196 373 3063
Fragments arranged by size:
3063 2196 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2476 2600 3695 5079 5347 1632
Size: 844 124 1095 1384 268 1917
Fragments arranged by size:
1917 1384 1095 844 268 124
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5435
AflIII A'CryG_T
Cuts at: 1203 4531 5508 1203
Size: 3328 977 1327
Fragments arranged by size:
3328 1327 977
AgeI A'CCGG_T
Cuts at: 4463 4463
Size: 5632
AhdI GACnn_n'nnGTC
Cuts at: 2766 4361 2766
Size: 1595 4037
AleI CACnn'nnGTG
Cuts at: 1166 2618 1166
Size: 1452 4180
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5632
AlwNI CAG_nnn'CTG
Cuts at: 3375 4947 3375
Size: 1572 4060
ApaLI G'TGCA_C
Cuts at: 2215 3689 4845 2215
Size: 1474 1156 3002
Fragments arranged by size:
3002 1474 1156
ApoI r'AATT_y
Cuts at: 1493 2941 3167 3319 5428 1493
Size: 1448 226 152 2109 1697
Fragments arranged by size:
2109 1697 1448 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4186 160
Size: 872 3154 1606
Fragments arranged by size:
3154 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5632
AvaI C'yCGr_G
Cuts at: 1870 1943 2957 1870
Size: 73 1014 4545
Fragments arranged by size:
4545 1014 73
BamHI G'GATC_C
Cuts at: 2962 3329 2962
Size: 367 5265
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2012 2361 2953 4408 618
Size: 325 217 627 225 349 592 1455 1842
Fragments arranged by size:
1842 1455 627 592 349 325 225 217
BanII G_rGCy'C
Cuts at: 1391 2951 1391
Size: 1560 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2560 928
Size: 971 661 4000
Fragments arranged by size:
4000 971 661
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3823 3857 1903
Size: 34 1886 34 3678
Fragments arranged by size:
3678 1886 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3524 4734 3524
Size: 1210 4422
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5632
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4243 136
Size: 107 122 71 631 605 2571 1525
Fragments arranged by size:
2571 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5627 5627
Size: 5632
BlpI GC'TnA_GC
Cuts at: 3049 3049
Size: 5632
Bme1580I G_kGCm'C
Cuts at: 1790 2219 2377 2752 3693 4849 1790
Size: 429 158 375 941 1156 2573
Fragments arranged by size:
2573 1156 941 429 375 158
BmgBI CAC'GTC
Cuts at: 1318 2620 1318
Size: 1302 4330
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3363 4321 442
Size: 416 53 83 399 24 1946 958 1753
Fragments arranged by size:
1946 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5632
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2017 2777 4292 1239
Size: 778 760 1515 2579
Fragments arranged by size:
2579 1515 778 760
Bpu10I CC'TnA_GC
Cuts at: 2889 2889
Size: 5632
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3757 4622 4920 3757
Size: 865 298 4469
Fragments arranged by size:
4469 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2159 4295 882
Size: 617 660 2136 2219
Fragments arranged by size:
2219 2136 660 617
BsaAI yAC'GTr
Cuts at: 493 1204 2707 3423 493
Size: 711 1503 716 2702
Fragments arranged by size:
2702 1503 716 711
BsaBI GATnn'nnATC
Cuts at: 5626 5626
Size: 5632
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2088 2820 2861 3439
Size: 53 83 186 936 555 732 41 578
Cuts at: 3439 3821 4456 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 732 635 578 555 382 186
83 53 41
BsaWI w'CCGG_w
Cuts at: 4065 4463 4737 4884 5295 4065
Size: 398 274 147 411 4402
Fragments arranged by size:
4402 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5407
Fragments arranged by size:
5407 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5632
BseYI C'CCAG_C
Cuts at: 2053 2065 2536 4835 5331 2053
Size: 12 471 2299 496 2354
Fragments arranged by size:
2354 2299 496 471 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2219 2687 2850 1163
Size: 573 483 468 163 3945
Fragments arranged by size:
3945 573 483 468 163
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2409 3082 3843 4871 1540
Size: 275 186 408 673 761 1028 2301
Fragments arranged by size:
2301 1028 761 673 408 275 186
BsiHKAI G_wGCw'C
Cuts at: 2219 2501 3693 3778 4849 2219
Size: 282 1192 85 1071 3002
Fragments arranged by size:
3002 1192 1071 282 85
BsmI GAATG_Cn'
Cuts at: 3143 3236 3143
Size: 93 5539
BsmBI CGTCTCn'nnnn_
Cuts at: 2753 2753
Size: 5632
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2219 2377 2501 2752 2951 3693 3778
Size: 399 429 158 124 251 199 742 85
Cuts at: 3778 4849 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 429 399 251 199 158
124 85
BspEI T'CCGG_A
Cuts at: 5295 5295
Size: 5632
BspHI T'CATG_A
Cuts at: 3521 5251 3521
Size: 1730 3902
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2556 2987 844
Size: 1712 431 3489
Fragments arranged by size:
3489 1712 431
BsrBI CCG'CTC
Cuts at: 1394 1823 3519 1394
Size: 429 1696 3507
Fragments arranged by size:
3507 1696 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4127 4301 65
Size: 1183 2879 174 1396
Fragments arranged by size:
2879 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4276 4463 1700
Size: 110 2466 187 2869
Fragments arranged by size:
2869 2466 187 110
BsrGI T'GTAC_A
Cuts at: 96 2778 96
Size: 2682 2950
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5632
BssSI C'ACGA_G
Cuts at: 3692 4704 3692
Size: 1012 4620
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2242 2368 794
Size: 1448 126 4058
Fragments arranged by size:
4058 1448 126
BstBI TT'CG_AA
Cuts at: 4436 4436
Size: 5632
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5632
BstXI CCAn_nnnn'nTGG
Cuts at: 2338 2620 2674 2338
Size: 282 54 5296
Fragments arranged by size:
5296 282 54
BstYI r'GATC_y
Cuts at: 1972 2962 3329 3714 3731 5183 5269 5281 5627
Size: 990 367 385 17 1452 86 12 346
Cuts at: 5627 1972
Size: 1977
Fragments arranged by size:
1977 1452 990 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2337 2377 2520 2559 2874 513
Size: 552 1272 40 143 39 315 3271
Fragments arranged by size:
3271 1272 552 315 143 40 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2317 487
Size: 683 56 1091 3802
Fragments arranged by size:
3802 1091 683 56
BtsI GCAGTG_nn'
Cuts at: 2162 2736 3148 3942 3962 2162
Size: 574 412 794 20 3832
Fragments arranged by size:
3832 794 574 412 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5632
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2008 2043 529
Size: 35 1444 35 4118
Fragments arranged by size:
4118 1444 35 35
DraI TTT'AAA
Cuts at: 2936 2936
Size: 5632
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4639 809
Size: 507 3323 1802
Fragments arranged by size:
3323 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2196 2328 2352
Size: 54 1425 50 275 18 366 132 24
Cuts at: 2352 2406 2556 2841 2871 3079 3968 5597 8
Size: 54 150 285 30 208 889 1629 43
Fragments arranged by size:
1629 1425 889 366 285 275 208 150
132 54 54 50 43 30 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 2406 3079 1537
Size: 275 594 673 4090
Fragments arranged by size:
4090 673 594 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3562 1353
Size: 2209 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4219 4593 4739 387
Size: 1064 138 2630 374 146 1280
Fragments arranged by size:
2630 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2017 2476 2600 2777 3695 4292 5079
Size: 393 385 459 124 177 918 597 787
Cuts at: 5079 5347 1239
Size: 268 1524
Fragments arranged by size:
1524 918 787 597 459 393 385 268
177 124
EcoRI G'AATT_C
Cuts at: 2941 3319 2941
Size: 378 5254
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2086 2086
Size: 5632
FspI TGC'GCA
Cuts at: 4138 4138
Size: 5632
HaeII r_GCGC'y
Cuts at: 1678 2129 4779 1678
Size: 451 2650 2531
Fragments arranged by size:
2650 2531 451
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2752 2784 4273 4305 4347
Size: 32 135 32 1180 32 1489 32 42
Cuts at: 4347 4379 1373
Size: 32 2626
Fragments arranged by size:
2626 1489 1180 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2976 3222 669
Size: 988 281 1038 246 3079
Fragments arranged by size:
3079 1038 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5632
HpaI GTT'AAC
Cuts at: 3222 3222
Size: 5632
KpnI G_GTAC'C
Cuts at: 2957 2957
Size: 5632
MfeI C'AATT_G
Cuts at: 3231 3231
Size: 5632
MluI A'CGCG_T
Cuts at: 5508 5508
Size: 5632
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4746 4930 1305
Size: 3441 184 2007
Fragments arranged by size:
3441 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2843 10
Size: 54 1425 343 1011 2799
Fragments arranged by size:
2799 1425 1011 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 2336 2618 3590 3949 4108 518
Size: 648 1011 159 282 972 359 159 2042
Fragments arranged by size:
2042 1011 972 648 359 282 159 159
MspA1I CmG'CkG
Cuts at: 1606 2266 2368 2379 2533 3048 3725 4873 5118
Size: 660 102 11 154 515 677 1148 245
Cuts at: 5118 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 660 515 245 154 102
11
NcoI C'CATG_G
Cuts at: 513 1065 2520 513
Size: 552 1455 3625
Fragments arranged by size:
3625 1455 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5632
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5632
NotI GC'GGCC_GC
Cuts at: 3079 3079
Size: 5632
NspI r_CATG'y
Cuts at: 1132 1229 2152 2990 4535 1132
Size: 97 923 838 1545 2229
Fragments arranged by size:
2229 1545 923 838 97
PasI CC'CwG_GG
Cuts at: 1348 2281 1348
Size: 933 4699
PciI A'CATG_T
Cuts at: 4531 4531
Size: 5632
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5632
PfoI T'CCnGG_A
Cuts at: 2754 2853 2754
Size: 99 5533
PmeI GTTT'AAAC
Cuts at: 2936 2936
Size: 5632
PmlI CAC'GTG
Cuts at: 2707 2707
Size: 5632
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3701 3733 5214 5246 3701
Size: 32 1481 32 4087
Fragments arranged by size:
4087 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5632
PsiI TTA'TAA
Cuts at: 3202 3202
Size: 5632
PstI C_TGCA'G
Cuts at: 830 2984 830
Size: 2154 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5632
SacII CC_GC'GG
Cuts at: 2380 2380
Size: 5632
SalI G'TCGA_C
Cuts at: 1936 2974 1936
Size: 1038 4594
SbfI CC_TGCA'GG
Cuts at: 2984 2984
Size: 5632
ScaI AGT'ACT
Cuts at: 3880 5592 3880
Size: 1712 3920
SfcI C'TryA_G
Cuts at: 826 1045 2980 4115 4796 4987 826
Size: 219 1935 1135 681 191 1471
Fragments arranged by size:
1935 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5632
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5632
SmaI CCC'GGG
Cuts at: 1872 2959 1872
Size: 1087 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3736 4637 4899 820
Size: 197 926 1793 901 262 1553
Fragments arranged by size:
1793 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3423 493
Size: 2930 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5632
SphI G_CATG'C
Cuts at: 1132 2152 2990 1132
Size: 1020 838 3774
Fragments arranged by size:
3774 1020 838
SspI AAT'ATT
Cuts at: 5 52 3556 5
Size: 47 3504 2081
Fragments arranged by size:
3504 2081 47
StuI AGG'CCT
Cuts at: 1517 2106 1517
Size: 589 5043
StyI C'CwwG_G
Cuts at: 513 1065 2520 2844 3071 513
Size: 552 1455 324 227 3074
Fragments arranged by size:
3074 1455 552 324 227
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2122 2416 2626 3659 3676 3829 4014
Size: 1255 542 294 210 1033 17 153 185
Cuts at: 4014 4459 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1033 542 445 294 210 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2778 3878 5590
Size: 274 80 33 51 1443 801 1100 1712
Cuts at: 5590 96
Size: 138
Fragments arranged by size:
1712 1443 1100 801 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2731 2763 2900 2932 5592 5624 2731
Size: 32 137 32 2660 32 2739
Fragments arranged by size:
2739 2660 137 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5632
XbaI T'CTAG_A
Cuts at: 2968 2968
Size: 5632
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2191 2812 2191
Size: 621 5011
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5632
XmaI C'CCGG_G
Cuts at: 1870 2957 1870
Size: 1087 4545
XmnI GAAnn'nnTTC
Cuts at: 3761 3761
Size: 5632
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3440 276
Size: 53 83 186 936 1906 2468
Fragments arranged by size:
2468 1906 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI
PvuI SacII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SanDI SapI SexAI SfiI SfoI
SrfI SwaI