Restriction map for
pFN21AE9408
(Circular) (Six-base) MAPSORT of: pFN21AE9408.seq Check: 8238 from: 1 to: 5779
pFN21AE9408 5779 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2438 2438
Size: 5779
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3589 278
Size: 53 83 186 936 2053 2468
Fragments arranged by size:
2468 2053 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2838 2892 3122 1937
Size: 901 54 230 4594
Fragments arranged by size:
4594 901 230 54
Acc65I G'GTAC_C
Cuts at: 3100 3100
Size: 5779
AclI AA'CG_TT
Cuts at: 1563 3906 4279 1563
Size: 2343 373 3063
Fragments arranged by size:
3063 2343 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2490 3842 5226 5494 1632
Size: 858 1352 1384 268 1917
Fragments arranged by size:
1917 1384 1352 858 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5582
AflIII A'CryG_T
Cuts at: 1203 4678 5655 1203
Size: 3475 977 1327
Fragments arranged by size:
3475 1327 977
AgeI A'CCGG_T
Cuts at: 4610 4610
Size: 5779
AhdI GACnn_n'nnGTC
Cuts at: 4508 4508
Size: 5779
AleI CACnn'nnGTG
Cuts at: 1166 2060 1166
Size: 894 4885
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5779
AlwNI CAG_nnn'CTG
Cuts at: 2358 2731 3058 3522 5094 2358
Size: 373 327 464 1572 3043
Fragments arranged by size:
3043 1572 464 373 327
ApaI G_GGCC'C
Cuts at: 2031 3072 2031
Size: 1041 4738
ApaLI G'TGCA_C
Cuts at: 2180 2667 3836 4992 2180
Size: 487 1169 1156 2967
Fragments arranged by size:
2967 1169 1156 487
ApoI r'AATT_y
Cuts at: 1493 3088 3314 3466 5575 1493
Size: 1595 226 152 2109 1697
Fragments arranged by size:
2109 1697 1595 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4333 160
Size: 872 3301 1606
Fragments arranged by size:
3301 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5779
AvaI C'yCGr_G
Cuts at: 1870 1943 2250 3071 3104 1870
Size: 73 307 821 33 4545
Fragments arranged by size:
4545 821 307 73 33
BamHI G'GATC_C
Cuts at: 3109 3476 3109
Size: 367 5412
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2098 2144 2348 2540 3100
Size: 325 217 627 311 46 204 192 560
Cuts at: 3100 4555 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 560 325 311 217 204
192 46
BanII G_rGCy'C
Cuts at: 1391 2031 2964 2999 3008 3072 3098 1391
Size: 640 933 35 9 64 26 4072
Fragments arranged by size:
4072 933 640 64 35 26 9
BbeI G_GCGC'C
Cuts at: 2352 2352
Size: 5779
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2227 928
Size: 971 328 4480
Fragments arranged by size:
4480 971 328
BbvCI CC'TCA_GC
Cuts at: 2858 2858
Size: 5779
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3970 4004 1903
Size: 34 50 34 1949 34 3678
Fragments arranged by size:
3678 1949 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3671 4881 3671
Size: 1210 4569
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5779
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2940 4390 136
Size: 107 122 71 631 605 1268 1450 1525
Fragments arranged by size:
1525 1450 1268 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5774 5774
Size: 5779
BlpI GC'TnA_GC
Cuts at: 3196 3196
Size: 5779
Bme1580I G_kGCm'C
Cuts at: 1790 2031 2103 2184 2671 3072 3840 4996 1790
Size: 241 72 81 487 401 768 1156 2573
Fragments arranged by size:
2573 1156 768 487 401 241 81 72
BmgBI CAC'GTC
Cuts at: 1318 2383 2977 1318
Size: 1065 594 4120
Fragments arranged by size:
4120 1065 594
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3510 4468 442
Size: 416 53 83 399 24 2093 958 1753
Fragments arranged by size:
2093 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5779
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2004 2114 2633 4439 1239
Size: 765 110 519 1806 2579
Fragments arranged by size:
2579 1806 765 519 110
Bpu10I CC'TnA_GC
Cuts at: 2858 3007 2858
Size: 149 5630
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3904 4769 5067 3904
Size: 865 298 4616
Fragments arranged by size:
4616 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4442 882
Size: 617 2943 2219
Fragments arranged by size:
2943 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2845 3570 493
Size: 711 1641 725 2702
Fragments arranged by size:
2702 1641 725 711
BsaBI GATnn'nnATC
Cuts at: 5773 5773
Size: 5779
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2349 3586 3968 4603
Size: 53 83 186 936 816 1237 382 635
Cuts at: 4603 275
Size: 1451
Fragments arranged by size:
1451 1237 936 816 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4212 4610 4884 5031 5442 4212
Size: 398 274 147 411 4549
Fragments arranged by size:
4549 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2981 3011 1178
Size: 30 165 30 1578 30 3946
Fragments arranged by size:
3946 1578 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2147 1186
Size: 961 4818
BseYI C'CCAG_C
Cuts at: 2560 2963 4982 5478 2560
Size: 403 2019 496 2861
Fragments arranged by size:
2861 2019 496 403
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5206
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2037 2656 2681 3229 3990 5018
Size: 275 186 36 619 25 548 761 1028
Cuts at: 5018 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 619 548 275 186 36
25
BsiHKAI G_wGCw'C
Cuts at: 2184 2214 2671 2745 2999 3840 3925 4996 2184
Size: 30 457 74 254 841 85 1071 2967
Fragments arranged by size:
2967 1071 841 457 254 85 74 30
BsmI GAATG_Cn'
Cuts at: 3290 3383 3290
Size: 93 5686
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2103 2184 2214 2671 2745 2964
Size: 399 241 72 81 30 457 74 219
Cuts at: 2964 2999 3008 3072 3098 3840 3925 4996 1391
Size: 35 9 64 26 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 457 399 241 219 85
81 74 72 64 35 30 26 9
BspEI T'CCGG_A
Cuts at: 5442 5442
Size: 5779
BspHI T'CATG_A
Cuts at: 3668 5398 3668
Size: 1730 4049
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2438 3134 844
Size: 1594 696 3489
Fragments arranged by size:
3489 1594 696
BsrBI CCG'CTC
Cuts at: 1394 1823 3666 1394
Size: 429 1843 3507
Fragments arranged by size:
3507 1843 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4274 4448 65
Size: 1183 3026 174 1396
Fragments arranged by size:
3026 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2537 2577 2651 3032 4423 4610 1700
Size: 110 727 40 74 381 1391 187 2869
Fragments arranged by size:
2869 1391 727 381 187 110 74 40
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5779
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5779
BssSI C'ACGA_G
Cuts at: 2824 2831 3839 4851 2824
Size: 7 1008 1012 3752
Fragments arranged by size:
3752 1012 1008 7
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2731 794
Size: 1937 3842
BstBI TT'CG_AA
Cuts at: 4583 4583
Size: 5779
BstEII G'GTnAC_C
Cuts at: 1184 2293 1184
Size: 1109 4670
BstXI CCAn_nnnn'nTGG
Cuts at: 2092 2160 2197 2287 2092
Size: 68 37 90 5584
Fragments arranged by size:
5584 90 68 37
BstYI r'GATC_y
Cuts at: 1972 3109 3476 3861 3878 5330 5416 5428 5774
Size: 1137 367 385 17 1452 86 12 346
Cuts at: 5774 1972
Size: 1977
Fragments arranged by size:
1977 1452 1137 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2793 513
Size: 552 1728 3499
Fragments arranged by size:
3499 1728 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5040
Fragments arranged by size:
5040 683 56
BtsI GCAGTG_nn'
Cuts at: 2067 2407 3295 4089 4109 2067
Size: 340 888 794 20 3737
Fragments arranged by size:
3737 888 794 340 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5779
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5744
DraI TTT'AAA
Cuts at: 3083 3083
Size: 5779
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2888 4786 809
Size: 507 1572 1898 1802
Fragments arranged by size:
1898 1802 1572 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 2454 2532
Size: 54 1425 50 275 18 204 420 78
Cuts at: 2532 2653 2678 2790 2847 2941 3226 4115 5744
Size: 121 25 112 57 94 285 889 1629
Cuts at: 5744 8
Size: 43
Fragments arranged by size:
1629 1425 889 420 285 275 204 121
112 94 78 57 54 50 43 25
18
EagI C'GGCC_G
Cuts at: 1537 1812 2034 2653 2678 3226 1537
Size: 275 222 619 25 548 4090
Fragments arranged by size:
4090 619 548 275 222 25
EarI CTCTTCn'nnn_
Cuts at: 1353 2426 2942 3709 1353
Size: 1073 516 767 3423
Fragments arranged by size:
3423 1073 767 516
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2681 4366 4740 4886 387
Size: 1064 138 1092 1685 374 146 1280
Fragments arranged by size:
1685 1280 1092 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2997 2997
Size: 5779
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2004 2114 2490 2633 3842 4439 5226
Size: 393 372 110 376 143 1209 597 787
Cuts at: 5226 5494 1239
Size: 268 1524
Fragments arranged by size:
1524 1209 787 597 393 376 372 268
143 110
EcoNI CCTnn'n_nnAGG
Cuts at: 2503 3050 2503
Size: 547 5232
EcoO109I rG'GnC_Cy
Cuts at: 2027 2028 3055 2027
Size: 1 1027 4751
Fragments arranged by size:
4751 1027 1
EcoRI G'AATT_C
Cuts at: 3088 3466 3088
Size: 378 5401
FspI TGC'GCA
Cuts at: 4285 4285
Size: 5779
HaeII r_GCGC'y
Cuts at: 1678 2171 2228 2352 2710 2801 4926 1678
Size: 493 57 124 358 91 2125 2531
Fragments arranged by size:
2531 2125 493 358 124 91 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4420 4452 4494 4526 1373
Size: 32 135 32 2848 32 42 32 2626
Fragments arranged by size:
2848 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3123 3369 669
Size: 988 281 1185 246 3079
Fragments arranged by size:
3079 1185 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5779
HpaI GTT'AAC
Cuts at: 3369 3369
Size: 5779
KasI G'GCGC_C
Cuts at: 2348 2348
Size: 5779
KpnI G_GTAC'C
Cuts at: 3104 3104
Size: 5779
MfeI C'AATT_G
Cuts at: 3378 3378
Size: 5779
MluI A'CGCG_T
Cuts at: 5655 5655
Size: 5779
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4893 5077 1305
Size: 3588 184 2007
Fragments arranged by size:
3588 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2456 10
Size: 54 1425 343 624 3333
Fragments arranged by size:
3333 1425 624 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2060 2438 2642 3737 4096 4255 518
Size: 648 894 378 204 1095 359 159 2042
Fragments arranged by size:
2042 1095 894 648 378 359 204 159
MspA1I CmG'CkG
Cuts at: 1606 2024 2088 2484 2874 2970 3195 3872 5020
Size: 418 64 396 390 96 225 677 1148
Cuts at: 5020 5265 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 418 396 390 245 225
96 64
NaeI GCC'GGC
Cuts at: 2579 3034 2579
Size: 455 5324
NarI GG'CG_CC
Cuts at: 2349 2349
Size: 5779
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5227
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5779
NgoMIV G'CCGG_C
Cuts at: 2577 3032 2577
Size: 455 5324
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5779
NotI GC'GGCC_GC
Cuts at: 3226 3226
Size: 5779
NspI r_CATG'y
Cuts at: 1132 1229 2407 2815 2989 3137 4682 1132
Size: 97 1178 408 174 148 1545 2229
Fragments arranged by size:
2229 1545 1178 408 174 148 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5779
PciI A'CATG_T
Cuts at: 4678 4678
Size: 5779
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3058 1108
Size: 1950 3829
PfoI T'CCnGG_A
Cuts at: 2610 2610
Size: 5779
PmeI GTTT'AAAC
Cuts at: 3083 3083
Size: 5779
PmlI CAC'GTG
Cuts at: 2845 2845
Size: 5779
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3848 3880 5361 5393 3848
Size: 32 1481 32 4234
Fragments arranged by size:
4234 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5779
PsiI TTA'TAA
Cuts at: 3349 3349
Size: 5779
PspOMI G'GGCC_C
Cuts at: 2027 3068 2027
Size: 1041 4738
PstI C_TGCA'G
Cuts at: 830 3131 830
Size: 2301 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5779
PvuII CAG'CTG
Cuts at: 2874 2874
Size: 5779
SacI G_AGCT'C
Cuts at: 2999 2999
Size: 5779
SalI G'TCGA_C
Cuts at: 1936 3121 1936
Size: 1185 4594
SbfI CC_TGCA'GG
Cuts at: 3131 3131
Size: 5779
ScaI AGT'ACT
Cuts at: 4027 5739 4027
Size: 1712 4067
SexAI A'CCwGG_T
Cuts at: 2286 2286
Size: 5779
SfcI C'TryA_G
Cuts at: 826 1045 3127 4262 4943 5134 826
Size: 219 2082 1135 681 191 1471
Fragments arranged by size:
2082 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2940 2940
Size: 5779
SfoI GGC'GCC
Cuts at: 2350 2350
Size: 5779
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5779
SgrAI Cr'CCGG_yG
Cuts at: 1700 2537 1700
Size: 837 4942
SmaI CCC'GGG
Cuts at: 1872 2252 3106 1872
Size: 380 854 4545
Fragments arranged by size:
4545 854 380
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3883 4784 5046 820
Size: 197 926 1940 901 262 1553
Fragments arranged by size:
1940 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3570 493
Size: 3077 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5779
SphI G_CATG'C
Cuts at: 1132 2989 3137 1132
Size: 1857 148 3774
Fragments arranged by size:
3774 1857 148
SspI AAT'ATT
Cuts at: 5 52 3703 5
Size: 47 3651 2081
Fragments arranged by size:
3651 2081 47
StuI AGG'CCT
Cuts at: 1517 2157 2934 1517
Size: 640 777 4362
Fragments arranged by size:
4362 777 640
StyI C'CwwG_G
Cuts at: 513 1065 3000 3218 513
Size: 552 1935 218 3074
Fragments arranged by size:
3074 1935 552 218
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2312 3065 3806 3823 3976 4161 4606
Size: 1255 732 753 741 17 153 185 445
Cuts at: 4606 325
Size: 1498
Fragments arranged by size:
1498 1255 753 741 732 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2340 4025 5737
Size: 274 80 33 51 1443 363 1685 1712
Cuts at: 5737 96
Size: 138
Fragments arranged by size:
1712 1685 1443 363 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2981 3013 5739 5771 2981
Size: 32 2726 32 2989
Fragments arranged by size:
2989 2726 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2978 1316
Size: 1662 4117
XbaI T'CTAG_A
Cuts at: 3115 3115
Size: 5779
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2287 2287
Size: 5779
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5779
XmaI C'CCGG_G
Cuts at: 1870 2250 3104 1870
Size: 380 854 4545
Fragments arranged by size:
4545 854 380
XmnI GAAnn'nnTTC
Cuts at: 3908 3908
Size: 5779
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3587 276
Size: 53 83 186 936 2053 2468
Fragments arranged by size:
2468 2053 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI
NruI NsiI PacI PpuMI PspXI PsrI RsrII SacII
SanDI SapI SrfI SwaI