Restriction map for
pFN21AE9406
(Circular) (Six-base) MAPSORT of: pFN21AE9406.seq Check: 7129 from: 1 to: 6115
pFN21AE9406 6115 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2678 3262 2678
Size: 584 5531
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2805 3925 278
Size: 53 83 186 936 1269 1120 2468
Fragments arranged by size:
2468 1269 1120 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2805 2838 3458 1937
Size: 868 33 620 4594
Fragments arranged by size:
4594 868 620 33
Acc65I G'GTAC_C
Cuts at: 2418 3436 2418
Size: 1018 5097
AclI AA'CG_TT
Cuts at: 1563 4242 4615 1563
Size: 2679 373 3063
Fragments arranged by size:
3063 2679 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2473 3378 4178 5562 5830 1632
Size: 841 905 800 1384 268 1917
Fragments arranged by size:
1917 1384 905 841 800 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5918
AflIII A'CryG_T
Cuts at: 1203 2195 5014 5991 1203
Size: 992 2819 977 1327
Fragments arranged by size:
2819 1327 992 977
AgeI A'CCGG_T
Cuts at: 4946 4946
Size: 6115
AhdI GACnn_n'nnGTC
Cuts at: 4844 4844
Size: 6115
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6115
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2971 1184
Size: 1787 4328
AlwNI CAG_nnn'CTG
Cuts at: 3209 3279 3858 5430 3209
Size: 70 579 1572 3894
Fragments arranged by size:
3894 1572 579 70
ApaI G_GGCC'C
Cuts at: 3328 3328
Size: 6115
ApaLI G'TGCA_C
Cuts at: 4172 5328 4172
Size: 1156 4959
ApoI r'AATT_y
Cuts at: 1493 3424 3650 3802 5911 1493
Size: 1931 226 152 2109 1697
Fragments arranged by size:
2109 1931 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4669 160
Size: 872 3637 1606
Fragments arranged by size:
3637 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6115
AvaI C'yCGr_G
Cuts at: 1870 1943 2513 3397 3440 1870
Size: 73 570 884 43 4545
Fragments arranged by size:
4545 884 570 73 43
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2402 2435 2402
Size: 33 6082
BamHI G'GATC_C
Cuts at: 3061 3109 3157 3445 3812 3061
Size: 48 48 288 367 5364
Fragments arranged by size:
5364 367 288 48 48
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2033 2418 2427 3436 4891
Size: 325 217 627 246 385 9 1009 1455
Cuts at: 4891 618
Size: 1842
Fragments arranged by size:
1842 1455 1009 627 385 325 246 217
9
BanII G_rGCy'C
Cuts at: 1391 3328 3434 1391
Size: 1937 106 4072
Fragments arranged by size:
4072 1937 106
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2098 2500 928
Size: 971 199 402 4543
Fragments arranged by size:
4543 971 402 199
BbvCI CC'TCA_GC
Cuts at: 2378 2582 2873 2378
Size: 204 291 5620
Fragments arranged by size:
5620 291 204
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4306 4340 1903
Size: 34 2369 34 3678
Fragments arranged by size:
3678 2369 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4007 5217 4007
Size: 1210 4905
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6115
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2717 4726 136
Size: 107 122 71 631 605 1045 2009 1525
Fragments arranged by size:
2009 1525 1045 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6110 6110
Size: 6115
BlpI GC'TnA_GC
Cuts at: 2216 3120 3532 2216
Size: 904 412 4799
Fragments arranged by size:
4799 904 412
Bme1580I G_kGCm'C
Cuts at: 1790 2411 2430 2499 3273 3328 4176 5332 1790
Size: 621 19 69 774 55 848 1156 2573
Fragments arranged by size:
2573 1156 848 774 621 69 55 19
BmgBI CAC'GTC
Cuts at: 1318 2198 1318
Size: 880 5235
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2429 3043 3267
Size: 416 53 83 399 24 1012 614 224
Cuts at: 3267 3846 4804 442
Size: 579 958 1753
Fragments arranged by size:
1753 1012 958 614 579 416 399 224
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6115
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2612 3080 4775 1239
Size: 1373 468 1695 2579
Fragments arranged by size:
2579 1695 1373 468
Bpu10I CC'TnA_GC
Cuts at: 2378 2582 2873 2378
Size: 204 291 5620
Fragments arranged by size:
5620 291 204
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3350 4240 5105 5403 3350
Size: 890 865 298 4062
Fragments arranged by size:
4062 890 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4778 882
Size: 617 3279 2219
Fragments arranged by size:
3279 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2641 3906 493
Size: 711 1437 1265 2702
Fragments arranged by size:
2702 1437 1265 711
BsaBI GATnn'nnATC
Cuts at: 6109 6109
Size: 6115
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2802 3922 4304 4939
Size: 53 83 186 936 1269 1120 382 635
Cuts at: 4939 275
Size: 1451
Fragments arranged by size:
1451 1269 1120 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2910 4548 4946 5220 5367 5778 2910
Size: 1638 398 274 147 411 3247
Fragments arranged by size:
3247 1638 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5890
Fragments arranged by size:
5890 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2341 2585 2975 1186
Size: 1155 244 390 4326
Fragments arranged by size:
4326 1155 390 244
BseYI C'CCAG_C
Cuts at: 2094 2299 2772 3000 3072 3144 3168 3192 3216
Size: 205 473 228 72 72 24 24 24
Cuts at: 3216 3337 3371 5318 5814 2094
Size: 121 34 1947 496 2395
Fragments arranged by size:
2395 1947 496 473 228 205 121 72
72 34 24 24 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3234 1163
Size: 573 1498 4044
Fragments arranged by size:
4044 1498 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2389 2969 3107 3565 4326 5354
Size: 275 186 388 580 138 458 761 1028
Cuts at: 5354 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 580 458 388 275 186
138
BsiHKAI G_wGCw'C
Cuts at: 2646 2934 4176 4261 5332 2646
Size: 288 1242 85 1071 3429
Fragments arranged by size:
3429 1242 1071 288 85
BsmI GAATG_Cn'
Cuts at: 3626 3719 3626
Size: 93 6022
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2411 2430 2499 2646 2934 3273 3328
Size: 399 621 19 69 147 288 339 55
Cuts at: 3328 3434 4176 4261 5332 1391
Size: 106 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 621 399 339 288 147
106 85 69 55 19
BspEI T'CCGG_A
Cuts at: 2910 5778 2910
Size: 2868 3247
BspHI T'CATG_A
Cuts at: 4004 5734 4004
Size: 1730 4385
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2678 2826 3262 3470 844
Size: 1834 148 436 208 3489
Fragments arranged by size:
3489 1834 436 208 148
BsrBI CCG'CTC
Cuts at: 1394 1823 2965 4002 1394
Size: 429 1142 1037 3507
Fragments arranged by size:
3507 1142 1037 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4610 4784 65
Size: 1183 3362 174 1396
Fragments arranged by size:
3362 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2165 2462 4759 4946 1700
Size: 110 355 297 2297 187 2869
Fragments arranged by size:
2869 2297 355 297 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6115
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6115
BssSI C'ACGA_G
Cuts at: 4175 5187 4175
Size: 1012 5103
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3257 794
Size: 2463 3652
BstBI TT'CG_AA
Cuts at: 4919 4919
Size: 6115
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6115
BstYI r'GATC_y
Cuts at: 1972 2265 2274 3061 3109 3157 3445 3812 4197
Size: 293 9 787 48 48 288 367 385
Cuts at: 4197 4214 5666 5752 5764 6110 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 787 385 367 346 293 288
86 48 48 17 12 9
Bsu36I CC'TnA_GG
Cuts at: 2750 3078 2750
Size: 328 5787
BtgI C'CryG_G
Cuts at: 513 1065 2004 2016 3411 513
Size: 552 939 12 1395 3217
Fragments arranged by size:
3217 1395 939 552 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5376
Fragments arranged by size:
5376 683 56
BtsI GCAGTG_nn'
Cuts at: 3631 4425 4445 3631
Size: 794 20 5301
Fragments arranged by size:
5301 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6115
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6080
DraI TTT'AAA
Cuts at: 3419 3419
Size: 6115
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2731 3236 5122 809
Size: 507 1415 505 1886 1802
Fragments arranged by size:
1886 1802 1415 507 505
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2041 2163 2294
Size: 54 1425 50 275 18 211 122 131
Cuts at: 2294 2370 2478 2718 2895 2966 3562 4451 6080
Size: 76 108 240 177 71 596 889 1629
Cuts at: 6080 8
Size: 43
Fragments arranged by size:
1629 1425 889 596 275 240 211 177
131 122 108 76 71 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2966 3562 1537
Size: 275 1154 596 4090
Fragments arranged by size:
4090 1154 596 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2366 2492 4045 1353
Size: 1013 126 1553 3423
Fragments arranged by size:
3423 1553 1013 126
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2429 4702 5076 5222 387
Size: 1064 138 840 2273 374 146 1280
Fragments arranged by size:
2273 1280 1064 840 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2473 2612 3080 3378 4178 4775 5562
Size: 393 841 139 468 298 800 597 787
Cuts at: 5562 5830 1239
Size: 268 1524
Fragments arranged by size:
1524 841 800 787 597 468 393 298
268 139
EcoNI CCTnn'n_nnAGG
Cuts at: 3318 3318
Size: 6115
EcoO109I rG'GnC_Cy
Cuts at: 2049 2596 2830 3324 3325 3334 3407 2049
Size: 547 234 494 1 9 73 4757
Fragments arranged by size:
4757 547 494 234 73 9 1
EcoRI G'AATT_C
Cuts at: 3424 3802 3424
Size: 378 5737
FspI TGC'GCA
Cuts at: 4621 4621
Size: 6115
HaeII r_GCGC'y
Cuts at: 1678 2207 2405 5262 1678
Size: 529 198 2857 2531
Fragments arranged by size:
2857 2531 529 198
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2060 2092 2561 2593 2792
Size: 32 135 32 488 32 469 32 199
Cuts at: 2792 2824 3035 3067 4756 4788 4830 4862 1373
Size: 32 211 32 1689 32 42 32 2626
Fragments arranged by size:
2626 1689 488 469 211 199 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3459 3705 669
Size: 988 281 1521 246 3079
Fragments arranged by size:
3079 1521 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3288 748
Size: 2540 3575
HpaI GTT'AAC
Cuts at: 3705 3705
Size: 6115
KpnI G_GTAC'C
Cuts at: 2422 3440 2422
Size: 1018 5097
MfeI C'AATT_G
Cuts at: 3714 3714
Size: 6115
MluI A'CGCG_T
Cuts at: 5991 5991
Size: 6115
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2868 3044 3135 5229 5413 1305
Size: 1563 176 91 2094 184 2007
Fragments arranged by size:
2094 2007 1563 184 176 91
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2043 2480 2720 2897 10
Size: 54 1425 343 211 437 240 177 3228
Fragments arranged by size:
3228 1425 437 343 240 211 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2015 4073 4432 4591 518
Size: 648 849 2058 359 159 2042
Fragments arranged by size:
2058 2042 849 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2255 2299 2309 2391 2767 2776 2868 3130
Size: 649 44 10 82 376 9 92 262
Cuts at: 3130 3178 3531 4208 5356 5601 1606
Size: 48 353 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 649 376 353 262 245
92 82 48 44 10 9
NaeI GCC'GGC
Cuts at: 2167 2167
Size: 6115
NcoI C'CATG_G
Cuts at: 513 1065 2004 2016 513
Size: 552 939 12 4612
Fragments arranged by size:
4612 939 552 12
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6115
NgoMIV G'CCGG_C
Cuts at: 2165 2165
Size: 6115
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6115
NotI GC'GGCC_GC
Cuts at: 3562 3562
Size: 6115
NspI r_CATG'y
Cuts at: 1132 1229 3473 5018 1132
Size: 97 2244 1545 2229
Fragments arranged by size:
2244 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2423 3320 1348
Size: 1075 897 4143
Fragments arranged by size:
4143 1075 897
PciI A'CATG_T
Cuts at: 5014 5014
Size: 6115
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2144 1108
Size: 1036 5079
PmeI GTTT'AAAC
Cuts at: 3419 3419
Size: 6115
PmlI CAC'GTG
Cuts at: 2641 2641
Size: 6115
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4184 4216 5697 5729 4184
Size: 32 1481 32 4570
Fragments arranged by size:
4570 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2596 3334 2596
Size: 738 5377
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6115
PsiI TTA'TAA
Cuts at: 3685 3685
Size: 6115
PspOMI G'GGCC_C
Cuts at: 3324 3324
Size: 6115
PstI C_TGCA'G
Cuts at: 830 3467 830
Size: 2637 3478
PvuI CG_AT'CG
Cuts at: 2001 3107 2001
Size: 1106 5009
PvuII CAG'CTG
Cuts at: 2309 2767 2776 2868 3130 3178 2309
Size: 458 9 92 262 48 5246
Fragments arranged by size:
5246 458 262 92 48 9
SalI G'TCGA_C
Cuts at: 1936 3457 1936
Size: 1521 4594
SbfI CC_TGCA'GG
Cuts at: 3467 3467
Size: 6115
ScaI AGT'ACT
Cuts at: 4363 6075 4363
Size: 1712 4403
SexAI A'CCwGG_T
Cuts at: 2538 2538
Size: 6115
SfcI C'TryA_G
Cuts at: 826 1045 3463 4598 5279 5470 826
Size: 219 2418 1135 681 191 1471
Fragments arranged by size:
2418 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6115
SgrAI Cr'CCGG_yG
Cuts at: 1700 2462 1700
Size: 762 5353
SmaI CCC'GGG
Cuts at: 1872 3399 3442 1872
Size: 1527 43 4545
Fragments arranged by size:
4545 1527 43
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3329 4219 5120 5382 820
Size: 197 926 1386 890 901 262 1553
Fragments arranged by size:
1553 1386 926 901 890 262 197
SnaBI TAC'GTA
Cuts at: 493 3906 493
Size: 3413 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6115
SphI G_CATG'C
Cuts at: 1132 3473 1132
Size: 2341 3774
SspI AAT'ATT
Cuts at: 5 52 4039 5
Size: 47 3987 2081
Fragments arranged by size:
3987 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6115
StyI C'CwwG_G
Cuts at: 513 1065 2004 2016 2086 2128 3554 513
Size: 552 939 12 70 42 1426 3074
Fragments arranged by size:
3074 1426 939 552 70 42 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2021 2461 2663 4142 4159 4312 4497
Size: 1255 441 440 202 1479 17 153 185
Cuts at: 4497 4942 325
Size: 445 1498
Fragments arranged by size:
1498 1479 1255 445 441 440 202 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4361 6073 96
Size: 274 80 33 51 1443 2384 1712 138
Fragments arranged by size:
2384 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6075 6107 6075
Size: 32 6083
Tth111I GACn'n_nGTC
Cuts at: 1316 2102 2540 1316
Size: 786 438 4891
Fragments arranged by size:
4891 786 438
XbaI T'CTAG_A
Cuts at: 3451 3451
Size: 6115
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2149 2180 2149
Size: 31 6084
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6115
XmaI C'CCGG_G
Cuts at: 1870 3397 3440 1870
Size: 1527 43 4545
Fragments arranged by size:
4545 1527 43
XmnI GAAnn'nnTTC
Cuts at: 4244 4244
Size: 6115
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2803 3923 276
Size: 53 83 186 936 1269 1120 2468
Fragments arranged by size:
2468 1269 1120 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PfoI PspXI
PsrI RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI