Restriction map for
pFN21AE9404
(Circular) (Six-base) MAPSORT of: pFN21AE9404.seq Check: 8806 from: 1 to: 5746
pFN21AE9404 5746 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3556 278
Size: 53 83 186 936 2020 2468
Fragments arranged by size:
2468 2020 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3089 1937
Size: 1152 4594
Acc65I G'GTAC_C
Cuts at: 2868 3067 2868
Size: 199 5547
AclI AA'CG_TT
Cuts at: 1563 3873 4246 1563
Size: 2310 373 3063
Fragments arranged by size:
3063 2310 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2733 3809 5193 5461 1632
Size: 1101 1076 1384 268 1917
Fragments arranged by size:
1917 1384 1101 1076 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5549
AflIII A'CryG_T
Cuts at: 1203 4645 5622 1203
Size: 3442 977 1327
Fragments arranged by size:
3442 1327 977
AgeI A'CCGG_T
Cuts at: 4577 4577
Size: 5746
AhdI GACnn_n'nnGTC
Cuts at: 4475 4475
Size: 5746
AleI CACnn'nnGTG
Cuts at: 1166 2834 1166
Size: 1668 4078
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5746
AlwNI CAG_nnn'CTG
Cuts at: 3489 5061 3489
Size: 1572 4174
ApaLI G'TGCA_C
Cuts at: 3803 4959 3803
Size: 1156 4590
ApoI r'AATT_y
Cuts at: 1493 3055 3281 3433 5542 1493
Size: 1562 226 152 2109 1697
Fragments arranged by size:
2109 1697 1562 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4300 160
Size: 872 3268 1606
Fragments arranged by size:
3268 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5746
AvaI C'yCGr_G
Cuts at: 1870 1943 2775 3071 1870
Size: 73 832 296 4545
Fragments arranged by size:
4545 832 296 73
BamHI G'GATC_C
Cuts at: 3076 3443 3076
Size: 367 5379
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2020 2134 2627 2786 2868
Size: 325 217 627 233 114 493 159 82
Cuts at: 2868 3021 3067 4522 618
Size: 153 46 1455 1842
Fragments arranged by size:
1842 1455 627 493 325 233 217 159
153 114 82 46
BanII G_rGCy'C
Cuts at: 1391 2256 3065 1391
Size: 865 809 4072
Fragments arranged by size:
4072 865 809
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2380 928
Size: 971 481 4294
Fragments arranged by size:
4294 971 481
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3937 3971 1903
Size: 34 2000 34 3678
Fragments arranged by size:
3678 2000 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3638 4848 3638
Size: 1210 4536
BclI T'GATC_A
Cuts at: 1548 2811 1548
Size: 1263 4483
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2034 2195 2340
Size: 107 122 71 631 605 362 161 145
Cuts at: 2340 2504 4357 136
Size: 164 1853 1525
Fragments arranged by size:
1853 1525 631 605 362 164 161 145
122 107 71
BglII A'GATC_T
Cuts at: 5741 5741
Size: 5746
BlpI GC'TnA_GC
Cuts at: 2561 3163 2561
Size: 602 5144
Bme1580I G_kGCm'C
Cuts at: 1790 2039 2139 2630 2789 3807 4963 1790
Size: 249 100 491 159 1018 1156 2573
Fragments arranged by size:
2573 1156 1018 491 249 159 100
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5746
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3477 4435 442
Size: 416 53 83 399 24 2060 958 1753
Fragments arranged by size:
2060 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5746
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2311 2606 2743 4406 1239
Size: 1072 295 137 1663 2579
Fragments arranged by size:
2579 1663 1072 295 137
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2155 3871 4736 5034 2155
Size: 1716 865 298 2867
Fragments arranged by size:
2867 1716 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4409 882
Size: 617 2910 2219
Fragments arranged by size:
2910 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2824 3537 493
Size: 711 1620 713 2702
Fragments arranged by size:
2702 1620 713 711
BsaBI GATnn'nnATC
Cuts at: 5740 5740
Size: 5746
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3553 3935 4570 275
Size: 53 83 186 936 2020 382 635 1451
Fragments arranged by size:
2020 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4179 4577 4851 4998 5409 4179
Size: 398 274 147 411 4516
Fragments arranged by size:
4516 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5521
Fragments arranged by size:
5521 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2297 2314 2603 1186
Size: 1111 17 289 4329
Fragments arranged by size:
4329 1111 289 17
BseYI C'CCAG_C
Cuts at: 2138 2343 2350 2580 2631 4949 5445 2138
Size: 205 7 230 51 2318 496 2439
Fragments arranged by size:
2439 2318 496 230 205 51 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2383 2420 2680 1163
Size: 573 647 37 260 4229
Fragments arranged by size:
4229 647 573 260 37
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3196 3957 4985 1540
Size: 275 186 1195 761 1028 2301
Fragments arranged by size:
2301 1195 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2831 3807 3892 4963 2831
Size: 976 85 1071 3614
Fragments arranged by size:
3614 1071 976 85
BsmI GAATG_Cn'
Cuts at: 2370 3257 3350 2370
Size: 887 93 4766
Fragments arranged by size:
4766 887 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2039 2139 2256 2630 2789 2831 3065
Size: 399 249 100 117 374 159 42 234
Cuts at: 3065 3807 3892 4963 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 374 249 234 159
117 100 85 42
BspEI T'CCGG_A
Cuts at: 5409 5409
Size: 5746
BspHI T'CATG_A
Cuts at: 2928 3635 5365 2928
Size: 707 1730 3309
Fragments arranged by size:
3309 1730 707
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2271 2973 3101 844
Size: 1427 702 128 3489
Fragments arranged by size:
3489 1427 702 128
BsrBI CCG'CTC
Cuts at: 1394 1823 3633 1394
Size: 429 1810 3507
Fragments arranged by size:
3507 1810 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2706 4241 4415 65
Size: 1183 1458 1535 174 1396
Fragments arranged by size:
1535 1458 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2050 2976 4390 4577 1700
Size: 110 240 926 1414 187 2869
Fragments arranged by size:
2869 1414 926 240 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5746
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5746
BssSI C'ACGA_G
Cuts at: 3806 4818 3806
Size: 1012 4734
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5746
BstBI TT'CG_AA
Cuts at: 4550 4550
Size: 5746
BstEII G'GTnAC_C
Cuts at: 1184 2218 1184
Size: 1034 4712
BstXI CCAn_nnnn'nTGG
Cuts at: 2335 2335
Size: 5746
BstYI r'GATC_y
Cuts at: 1972 2727 3076 3443 3828 3845 5297 5383 5395
Size: 755 349 367 385 17 1452 86 12
Cuts at: 5395 5741 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 755 385 367 349 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2129 2129
Size: 5746
BtgI C'CryG_G
Cuts at: 513 1065 2004 2488 513
Size: 552 939 484 3771
Fragments arranged by size:
3771 939 552 484
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5007
Fragments arranged by size:
5007 683 56
BtsI GCAGTG_nn'
Cuts at: 2462 2514 3262 4056 4076 2462
Size: 52 748 794 20 4132
Fragments arranged by size:
4132 794 748 52 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5746
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5711
DraI TTT'AAA
Cuts at: 3050 3050
Size: 5746
DraIII CAC_nnn'GTG
Cuts at: 2806 2806
Size: 5746
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4753 809
Size: 507 3437 1802
Fragments arranged by size:
3437 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2048 3193 4082
Size: 54 1425 50 275 18 218 1145 889
Cuts at: 4082 5711 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1145 889 275 218 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3193 1537
Size: 275 1381 4090
Fragments arranged by size:
4090 1381 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3676 1353
Size: 2323 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4333 4707 4853 387
Size: 1064 138 2744 374 146 1280
Fragments arranged by size:
2744 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2311 2606 2733 2743 3809 4406 5193
Size: 393 679 295 127 10 1066 597 787
Cuts at: 5193 5461 1239
Size: 268 1524
Fragments arranged by size:
1524 1066 787 679 597 393 295 268
127 10
EcoO109I rG'GnC_Cy
Cuts at: 2125 2125
Size: 5746
EcoRI G'AATT_C
Cuts at: 3055 3433 3055
Size: 378 5368
FseI GG_CCGG'CC
Cuts at: 2054 2054
Size: 5746
FspI TGC'GCA
Cuts at: 4252 4252
Size: 5746
HaeII r_GCGC'y
Cuts at: 1678 4893 1678
Size: 3215 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2631 2663 4387 4419 4461
Size: 32 135 32 1059 32 1724 32 42
Cuts at: 4461 4493 1373
Size: 32 2626
Fragments arranged by size:
2626 1724 1059 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3090 3336 669
Size: 988 281 1152 246 3079
Fragments arranged by size:
3079 1152 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5746
HpaI GTT'AAC
Cuts at: 3336 3336
Size: 5746
KpnI G_GTAC'C
Cuts at: 2872 3071 2872
Size: 199 5547
MfeI C'AATT_G
Cuts at: 3345 3345
Size: 5746
MluI A'CGCG_T
Cuts at: 5622 5622
Size: 5746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4860 5044 1305
Size: 3555 184 2007
Fragments arranged by size:
3555 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3924
Fragments arranged by size:
3924 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2834 3704 4063 4222 518
Size: 648 1668 870 359 159 2042
Fragments arranged by size:
2042 1668 870 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2584 2983 3162 3839 4987 5232 1606
Size: 978 399 179 677 1148 245 2120
Fragments arranged by size:
2120 1148 978 677 399 245 179
NaeI GCC'GGC
Cuts at: 2052 2052
Size: 5746
NcoI C'CATG_G
Cuts at: 513 1065 2004 2488 513
Size: 552 939 484 3771
Fragments arranged by size:
3771 939 552 484
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5746
NgoMIV G'CCGG_C
Cuts at: 2050 2050
Size: 5746
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5746
NotI GC'GGCC_GC
Cuts at: 3193 3193
Size: 5746
NspI r_CATG'y
Cuts at: 1132 1229 2089 2317 3104 4649 1132
Size: 97 860 228 787 1545 2229
Fragments arranged by size:
2229 1545 860 787 228 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5746
PciI A'CATG_T
Cuts at: 4645 4645
Size: 5746
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5746
PfoI T'CCnGG_A
Cuts at: 3014 3014
Size: 5746
PmeI GTTT'AAAC
Cuts at: 3050 3050
Size: 5746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3815 3847 5328 5360 3815
Size: 32 1481 32 4201
Fragments arranged by size:
4201 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2125 2125
Size: 5746
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5746
PsiI TTA'TAA
Cuts at: 3316 3316
Size: 5746
PstI C_TGCA'G
Cuts at: 830 2188 3098 830
Size: 1358 910 3478
Fragments arranged by size:
3478 1358 910
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5746
PvuII CAG'CTG
Cuts at: 2584 2983 2584
Size: 399 5347
SalI G'TCGA_C
Cuts at: 1936 3088 1936
Size: 1152 4594
SbfI CC_TGCA'GG
Cuts at: 3098 3098
Size: 5746
ScaI AGT'ACT
Cuts at: 3994 5706 3994
Size: 1712 4034
SexAI A'CCwGG_T
Cuts at: 2864 2864
Size: 5746
SfcI C'TryA_G
Cuts at: 826 1045 2040 2184 3094 4229 4910 5101 826
Size: 219 995 144 910 1135 681 191 1471
Fragments arranged by size:
1471 1135 995 910 681 219 191 144
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5746
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5746
SmaI CCC'GGG
Cuts at: 1872 2777 3073 1872
Size: 905 296 4545
Fragments arranged by size:
4545 905 296
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2170 3850 4751 5013 820
Size: 197 926 227 1680 901 262 1553
Fragments arranged by size:
1680 1553 926 901 262 227 197
SnaBI TAC'GTA
Cuts at: 493 3537 493
Size: 3044 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5746
SphI G_CATG'C
Cuts at: 1132 2089 3104 1132
Size: 957 1015 3774
Fragments arranged by size:
3774 1015 957
SspI AAT'ATT
Cuts at: 5 52 3670 5
Size: 47 3618 2081
Fragments arranged by size:
3618 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5746
StyI C'CwwG_G
Cuts at: 513 1065 2004 2112 2488 3185 513
Size: 552 939 108 376 697 3074
Fragments arranged by size:
3074 939 697 552 376 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2327 2645 3773 3790 3943 4128 4573
Size: 1255 747 318 1128 17 153 185 445
Cuts at: 4573 325
Size: 1498
Fragments arranged by size:
1498 1255 1128 747 445 318 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2151 3992 5704
Size: 274 80 33 51 1443 174 1841 1712
Cuts at: 5704 96
Size: 138
Fragments arranged by size:
1841 1712 1443 274 174 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2996 3028 5706 5738 2996
Size: 32 2678 32 3004
Fragments arranged by size:
3004 2678 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5746
XbaI T'CTAG_A
Cuts at: 3082 3082
Size: 5746
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2639 2882 2639
Size: 243 5503
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5746
XmaI C'CCGG_G
Cuts at: 1870 2775 3071 1870
Size: 905 296 4545
Fragments arranged by size:
4545 905 296
XmnI GAAnn'nnTTC
Cuts at: 3875 3875
Size: 5746
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3554 276
Size: 53 83 186 936 2020 2468
Fragments arranged by size:
2468 2020 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI
EcoNI EcoRV FalI FspAI KasI NarI NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SfiI SfoI SrfI SwaI