Restriction map for
pFN21AE9403
(Circular) (Six-base) MAPSORT of: pFN21AE9403.seq Check: 4678 from: 1 to: 6187
pFN21AE9403 6187 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3997 278
Size: 53 83 186 936 2461 2468
Fragments arranged by size:
2468 2461 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3530 1937
Size: 1593 4594
Acc65I G'GTAC_C
Cuts at: 3508 3508
Size: 6187
AclI AA'CG_TT
Cuts at: 1563 4314 4687 1563
Size: 2751 373 3063
Fragments arranged by size:
3063 2751 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2119 2557 2818 2959 4250 5634 5902 1632
Size: 487 438 261 141 1291 1384 268 1917
Fragments arranged by size:
1917 1384 1291 487 438 268 261 141
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5990
AflIII A'CryG_T
Cuts at: 1203 5086 6063 1203
Size: 3883 977 1327
Fragments arranged by size:
3883 1327 977
AgeI A'CCGG_T
Cuts at: 5018 5018
Size: 6187
AhdI GACnn_n'nnGTC
Cuts at: 4916 4916
Size: 6187
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6187
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2322 2930 1184
Size: 1138 608 4441
Fragments arranged by size:
4441 1138 608
AlwNI CAG_nnn'CTG
Cuts at: 2692 3930 5502 2692
Size: 1238 1572 3377
Fragments arranged by size:
3377 1572 1238
ApaI G_GGCC'C
Cuts at: 2541 3042 3263 3388 2541
Size: 501 221 125 5340
Fragments arranged by size:
5340 501 221 125
ApaLI G'TGCA_C
Cuts at: 4244 5400 4244
Size: 1156 5031
ApoI r'AATT_y
Cuts at: 1493 3496 3722 3874 5983 1493
Size: 2003 226 152 2109 1697
Fragments arranged by size:
2109 2003 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4741 160
Size: 872 3709 1606
Fragments arranged by size:
3709 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6187
AvaI C'yCGr_G
Cuts at: 1870 1943 2507 2744 2754 3262 3512 1870
Size: 73 564 237 10 508 250 4545
Fragments arranged by size:
4545 564 508 250 237 73 10
AvrII C'CTAG_G
Cuts at: 3196 3196
Size: 6187
BamHI G'GATC_C
Cuts at: 3517 3884 3517
Size: 367 5820
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2069 2284 2518 2699 2749
Size: 325 217 627 282 215 234 181 50
Cuts at: 2749 3508 4963 618
Size: 759 1455 1842
Fragments arranged by size:
1842 1455 759 627 325 282 234 217
215 181 50
BanII G_rGCy'C
Cuts at: 1391 2541 3042 3263 3388 3506 1391
Size: 1150 501 221 125 118 4072
Fragments arranged by size:
4072 1150 501 221 125 118
BbeI G_GCGC'C
Cuts at: 2073 2288 2522 2703 2753 2073
Size: 215 234 181 50 5507
Fragments arranged by size:
5507 234 215 181 50
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3010 928
Size: 971 1111 4105
Fragments arranged by size:
4105 1111 971
BbvCI CC'TCA_GC
Cuts at: 3107 3107
Size: 6187
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4378 4412 1903
Size: 34 2441 34 3678
Fragments arranged by size:
3678 2441 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3469 4079 5289 3469
Size: 610 1210 4367
Fragments arranged by size:
4367 1210 610
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6187
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2094 2120 3077
Size: 107 122 71 631 605 422 26 957
Cuts at: 3077 3143 3383 4798 136
Size: 66 240 1415 1525
Fragments arranged by size:
1525 1415 957 631 605 422 240 122
107 71 66 26
BglII A'GATC_T
Cuts at: 6182 6182
Size: 6187
BlpI GC'TnA_GC
Cuts at: 2603 3604 2603
Size: 1001 5186
Bme1580I G_kGCm'C
Cuts at: 1790 2541 3042 3263 3388 4248 5404 1790
Size: 751 501 221 125 860 1156 2573
Fragments arranged by size:
2573 1156 860 751 501 221 125
BmgBI CAC'GTC
Cuts at: 1318 2929 3373 1318
Size: 1611 444 4132
Fragments arranged by size:
4132 1611 444
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2060 3918 4876
Size: 416 53 83 399 24 643 1858 958
Cuts at: 4876 442
Size: 1753
Fragments arranged by size:
1858 1753 958 643 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6187
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4847 1239
Size: 3608 2579
Bpu10I CC'TnA_GC
Cuts at: 2168 3107 2168
Size: 939 5248
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4312 5177 5475 4312
Size: 865 298 5024
Fragments arranged by size:
5024 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4850 882
Size: 617 3351 2219
Fragments arranged by size:
3351 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3978 493
Size: 711 2774 2702
Fragments arranged by size:
2774 2702 711
BsaBI GATnn'nnATC
Cuts at: 6181 6181
Size: 6187
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2070 2285 2519 2700
Size: 53 83 186 936 537 215 234 181
Cuts at: 2700 2750 3994 4376 5011 275
Size: 50 1244 382 635 1451
Fragments arranged by size:
1451 1244 936 635 537 382 234 215
186 181 83 53 50
BsaWI w'CCGG_w
Cuts at: 2796 3222 4620 5018 5292 5439 5850 2796
Size: 426 1398 398 274 147 411 3133
Fragments arranged by size:
3133 1398 426 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2295 2325 2786 2816 1178
Size: 30 165 30 892 30 461 30 4549
Fragments arranged by size:
4549 892 461 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 3095 3227 1186
Size: 838 1071 132 4146
Fragments arranged by size:
4146 1071 838 132
BseYI C'CCAG_C
Cuts at: 2158 2821 3033 3251 3316 3470 5390 5886 2158
Size: 663 212 218 65 154 1920 496 2459
Fragments arranged by size:
2459 1920 663 496 218 212 154 65
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5614
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2085 2124 2151 2267 2651 2671
Size: 275 186 84 39 27 116 384 20
Cuts at: 2671 2942 3637 4398 5426 1540
Size: 271 695 761 1028 2301
Fragments arranged by size:
2301 1028 761 695 384 275 271 186
116 84 39 27 20
BsiHKAI G_wGCw'C
Cuts at: 2130 4248 4333 5404 2130
Size: 2118 85 1071 2913
Fragments arranged by size:
2913 2118 1071 85
BsmI GAATG_Cn'
Cuts at: 3698 3791 3698
Size: 93 6094
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2130 2541 3042 3263 3388 3506 4248
Size: 399 340 411 501 221 125 118 742
Cuts at: 4248 4333 5404 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 501 411 399 340 221
125 118 85
BspEI T'CCGG_A
Cuts at: 5850 5850
Size: 6187
BspHI T'CATG_A
Cuts at: 3009 4076 5806 3009
Size: 1067 1730 3390
Fragments arranged by size:
3390 1730 1067
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2871 3542 844
Size: 2027 671 3489
Fragments arranged by size:
3489 2027 671
BsrBI CCG'CTC
Cuts at: 1394 1823 2201 4074 1394
Size: 429 378 1873 3507
Fragments arranged by size:
3507 1873 429 378
BsrDI GCAATG_nn'
Cuts at: 65 1248 4682 4856 65
Size: 1183 3434 174 1396
Fragments arranged by size:
3434 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2084 2397 2941 3312 3433 4831 5018
Size: 110 274 313 544 371 121 1398 187
Cuts at: 5018 1700
Size: 2869
Fragments arranged by size:
2869 1398 544 371 313 274 187 121
110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6187
BssHII G'CGCG_C
Cuts at: 1925 2679 2957 1925
Size: 754 278 5155
Fragments arranged by size:
5155 754 278
BssSI C'ACGA_G
Cuts at: 2578 4247 5259 2578
Size: 1669 1012 3506
Fragments arranged by size:
3506 1669 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6187
BstBI TT'CG_AA
Cuts at: 3295 4991 3295
Size: 1696 4491
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6187
BstXI CCAn_nnnn'nTGG
Cuts at: 3316 3361 3316
Size: 45 6142
BstYI r'GATC_y
Cuts at: 1972 2449 3517 3884 4269 4286 5738 5824 5836
Size: 477 1068 367 385 17 1452 86 12
Cuts at: 5836 6182 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1068 477 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2079 2109 2115 2142 2151 2532
Size: 552 939 75 30 6 27 9 381
Cuts at: 2532 2654 2782 513
Size: 122 128 3918
Fragments arranged by size:
3918 939 552 381 128 122 75 30
27 9 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5448
Fragments arranged by size:
5448 683 56
BtsI GCAGTG_nn'
Cuts at: 2316 3450 3703 4497 4517 2316
Size: 1134 253 794 20 3986
Fragments arranged by size:
3986 1134 794 253 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6187
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6152
DraI TTT'AAA
Cuts at: 3491 3491
Size: 6187
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5194 809
Size: 507 3878 1802
Fragments arranged by size:
3878 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2082 2112 2121
Size: 54 1425 50 275 18 252 30 9
Cuts at: 2121 2264 2648 2668 2939 2991 3063 3117 3351
Size: 143 384 20 271 52 72 54 234
Cuts at: 3351 3375 3634 4523 6152 8
Size: 24 259 889 1629 43
Fragments arranged by size:
1629 1425 889 384 275 271 259 252
234 143 72 54 54 52 50 43
30 24 20 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 2082 2121 2264 2648 2668 2939 3634
Size: 275 270 39 143 384 20 271 695
Cuts at: 3634 1537
Size: 4090
Fragments arranged by size:
4090 695 384 275 271 270 143 39
20
EarI CTCTTCn'nnn_
Cuts at: 1353 2501 3346 4117 1353
Size: 1148 845 771 3423
Fragments arranged by size:
3423 1148 845 771
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2493 4774 5148 5294 387
Size: 1064 138 904 2281 374 146 1280
Fragments arranged by size:
2281 1280 1064 904 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2119 2557 2818 2959 4250 4847 5634
Size: 393 487 438 261 141 1291 597 787
Cuts at: 5634 5902 1239
Size: 268 1524
Fragments arranged by size:
1524 1291 787 597 487 438 393 268
261 141
EcoNI CCTnn'n_nnAGG
Cuts at: 3328 3328
Size: 6187
EcoO109I rG'GnC_Cy
Cuts at: 2537 2538 3038 3039 3094 3259 3321 2537
Size: 1 500 1 55 165 62 5403
Fragments arranged by size:
5403 500 165 62 55 1 1
EcoRI G'AATT_C
Cuts at: 3496 3874 3496
Size: 378 5809
FseI GG_CCGG'CC
Cuts at: 2088 2088
Size: 6187
FspI TGC'GCA
Cuts at: 2987 4693 2987
Size: 1706 4481
FspAI rTGC'GCAy
Cuts at: 2987 2987
Size: 6187
HaeII r_GCGC'y
Cuts at: 1678 2073 2159 2288 2327 2465 2522 2615 2703
Size: 395 86 129 39 138 57 93 88
Cuts at: 2703 2753 5334 1678
Size: 50 2581 2531
Fragments arranged by size:
2581 2531 395 138 129 93 88 86
57 50 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4828 4860 4902 4934 1373
Size: 32 135 32 3256 32 42 32 2626
Fragments arranged by size:
3256 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3531 3777 669
Size: 988 281 1593 246 3079
Fragments arranged by size:
3079 1593 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6187
HpaI GTT'AAC
Cuts at: 3777 3777
Size: 6187
KasI G'GCGC_C
Cuts at: 2069 2284 2518 2699 2749 2069
Size: 215 234 181 50 5507
Fragments arranged by size:
5507 234 215 181 50
KpnI G_GTAC'C
Cuts at: 3512 3512
Size: 6187
MfeI C'AATT_G
Cuts at: 3786 3786
Size: 6187
MluI A'CGCG_T
Cuts at: 6063 6063
Size: 6187
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2604 2904 3369 5301 5485 1305
Size: 1299 300 465 1932 184 2007
Fragments arranged by size:
2007 1932 1299 465 300 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3377 10
Size: 54 1425 343 1545 2820
Fragments arranged by size:
2820 1545 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3188 4145 4504 4663 518
Size: 648 2022 957 359 159 2042
Fragments arranged by size:
2042 2022 957 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2081 2111 2153 2195 2290 2534 2656 2689
Size: 475 30 42 42 95 244 122 33
Cuts at: 2689 2821 3357 3603 4280 5428 5673 1606
Size: 132 536 246 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 536 475 246 245 244
132 122 95 42 42 33 30
NaeI GCC'GGC
Cuts at: 2086 2086
Size: 6187
NarI GG'CG_CC
Cuts at: 2070 2285 2519 2700 2750 2070
Size: 215 234 181 50 5507
Fragments arranged by size:
5507 234 215 181 50
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4696
Fragments arranged by size:
4696 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6187
NgoMIV G'CCGG_C
Cuts at: 2084 2084
Size: 6187
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6187
NotI GC'GGCC_GC
Cuts at: 2264 2648 3634 2264
Size: 384 986 4817
Fragments arranged by size:
4817 986 384
NspI r_CATG'y
Cuts at: 1132 1229 3545 5090 1132
Size: 97 2316 1545 2229
Fragments arranged by size:
2316 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2716 2733 1348
Size: 1368 17 4802
Fragments arranged by size:
4802 1368 17
PciI A'CATG_T
Cuts at: 5086 5086
Size: 6187
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6187
PfoI T'CCnGG_A
Cuts at: 2487 2487
Size: 6187
PmeI GTTT'AAAC
Cuts at: 3491 3491
Size: 6187
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4256 4288 5769 5801 4256
Size: 32 1481 32 4642
Fragments arranged by size:
4642 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6187
PsiI TTA'TAA
Cuts at: 3757 3757
Size: 6187
PspOMI G'GGCC_C
Cuts at: 2537 3038 3259 3384 2537
Size: 501 221 125 5340
Fragments arranged by size:
5340 501 221 125
PstI C_TGCA'G
Cuts at: 830 2311 2688 3539 830
Size: 1481 377 851 3478
Fragments arranged by size:
3478 1481 851 377
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6187
PvuII CAG'CTG
Cuts at: 2195 2689 2821 2195
Size: 494 132 5561
Fragments arranged by size:
5561 494 132
SacII CC_GC'GG
Cuts at: 2082 2112 2154 2535 2657 2082
Size: 30 42 381 122 5612
Fragments arranged by size:
5612 381 122 42 30
SalI G'TCGA_C
Cuts at: 1936 3529 1936
Size: 1593 4594
SbfI CC_TGCA'GG
Cuts at: 3539 3539
Size: 6187
ScaI AGT'ACT
Cuts at: 4435 6147 4435
Size: 1712 4475
SfcI C'TryA_G
Cuts at: 826 1045 2307 2684 3535 4670 5351 5542 826
Size: 219 1262 377 851 1135 681 191 1471
Fragments arranged by size:
1471 1262 1135 851 681 377 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2120 3143 3383 2120
Size: 1023 240 4924
Fragments arranged by size:
4924 1023 240
SfoI GGC'GCC
Cuts at: 2071 2286 2520 2701 2751 2071
Size: 215 234 181 50 5507
Fragments arranged by size:
5507 234 215 181 50
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6187
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6187
SmaI CCC'GGG
Cuts at: 1872 2509 2756 3264 3514 1872
Size: 637 247 508 250 4545
Fragments arranged by size:
4545 637 508 250 247
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4291 5192 5454 820
Size: 197 926 2348 901 262 1553
Fragments arranged by size:
2348 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3978 493
Size: 3485 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6187
SphI G_CATG'C
Cuts at: 1132 3545 1132
Size: 2413 3774
SspI AAT'ATT
Cuts at: 5 52 4111 5
Size: 47 4059 2081
Fragments arranged by size:
4059 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6187
StyI C'CwwG_G
Cuts at: 513 1065 2004 2218 2359 3196 3626 513
Size: 552 939 214 141 837 430 3074
Fragments arranged by size:
3074 939 837 552 430 214 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4214 4231 4384 4569 5014 325
Size: 1255 2634 17 153 185 445 1498
Fragments arranged by size:
2634 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3403 4433 6145
Size: 274 80 33 51 1443 1426 1030 1712
Cuts at: 6145 96
Size: 138
Fragments arranged by size:
1712 1443 1426 1030 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2295 2327 6147 6179 2295
Size: 32 3820 32 2303
Fragments arranged by size:
3820 2303 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6187
XbaI T'CTAG_A
Cuts at: 3523 3523
Size: 6187
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2605 2605
Size: 6187
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6187
XmaI C'CCGG_G
Cuts at: 1870 2507 2754 3262 3512 1870
Size: 637 247 508 250 4545
Fragments arranged by size:
4545 637 508 250 247
XmnI GAAnn'nnTTC
Cuts at: 4316 4316
Size: 6187
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3995 276
Size: 53 83 186 936 2461 2468
Fragments arranged by size:
2468 2461 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI NruI
NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI
SanDI SapI SexAI SrfI SwaI