Restriction map for
pFN21AE9402
(Circular) (Six-base) MAPSORT of: pFN21AE9402.seq Check: 8202 from: 1 to: 5707
pFN21AE9402 5707 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2573 2573
Size: 5707
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3517 278
Size: 53 83 186 936 1981 2468
Fragments arranged by size:
2468 1981 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3050 1937
Size: 1113 4594
Acc65I G'GTAC_C
Cuts at: 3028 3028
Size: 5707
AclI AA'CG_TT
Cuts at: 1563 3834 4207 1563
Size: 2271 373 3063
Fragments arranged by size:
3063 2271 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2039 2309 2630 3770 5154 5422 1632
Size: 407 270 321 1140 1384 268 1917
Fragments arranged by size:
1917 1384 1140 407 321 270 268
AfeI AGC'GCT
Cuts at: 2937 2937
Size: 5707
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5510
AflIII A'CryG_T
Cuts at: 1203 4606 5583 1203
Size: 3403 977 1327
Fragments arranged by size:
3403 1327 977
AgeI A'CCGG_T
Cuts at: 4538 4538
Size: 5707
AhdI GACnn_n'nnGTC
Cuts at: 4436 4436
Size: 5707
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5707
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5707
AlwNI CAG_nnn'CTG
Cuts at: 2123 3450 5022 2123
Size: 1327 1572 2808
Fragments arranged by size:
2808 1572 1327
ApaLI G'TGCA_C
Cuts at: 3764 4920 3764
Size: 1156 4551
ApoI r'AATT_y
Cuts at: 1493 3016 3242 3394 5503 1493
Size: 1523 226 152 2109 1697
Fragments arranged by size:
2109 1697 1523 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4261 160
Size: 872 3229 1606
Fragments arranged by size:
3229 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5707
AvaI C'yCGr_G
Cuts at: 1870 1943 2043 2097 2164 3032 1870
Size: 73 100 54 67 868 4545
Fragments arranged by size:
4545 868 100 73 67 54
BamHI G'GATC_C
Cuts at: 3037 3404 3037
Size: 367 5340
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3028 4483 618
Size: 325 217 627 1241 1455 1842
Fragments arranged by size:
1842 1455 1241 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2130 3026 1391
Size: 739 896 4072
Fragments arranged by size:
4072 896 739
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2019 928
Size: 971 120 4616
Fragments arranged by size:
4616 971 120
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3898 3932 1903
Size: 34 1961 34 3678
Fragments arranged by size:
3678 1961 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3599 4809 3599
Size: 1210 4497
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5707
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4318 136
Size: 107 122 71 631 605 2646 1525
Fragments arranged by size:
2646 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2661 5702 2661
Size: 3041 2666
BlpI GC'TnA_GC
Cuts at: 3124 3124
Size: 5707
Bme1580I G_kGCm'C
Cuts at: 1790 3768 4924 1790
Size: 1978 1156 2573
Fragments arranged by size:
2573 1978 1156
BmgBI CAC'GTC
Cuts at: 1318 2724 1318
Size: 1406 4301
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2991 3438 4396
Size: 416 53 83 399 24 1574 447 958
Cuts at: 4396 442
Size: 1753
Fragments arranged by size:
1753 1574 958 447 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5707
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2095 2095
Size: 5707
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4367 1239
Size: 3128 2579
Bpu10I CC'TnA_GC
Cuts at: 2124 2124
Size: 5707
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3832 4697 4995 3832
Size: 865 298 4544
Fragments arranged by size:
4544 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2758 4370 882
Size: 617 1259 1612 2219
Fragments arranged by size:
2219 1612 1259 617
BsaAI yAC'GTr
Cuts at: 493 1204 3498 493
Size: 711 2294 2702
Fragments arranged by size:
2702 2294 711
BsaBI GATnn'nnATC
Cuts at: 5701 5701
Size: 5707
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3514 3896 4531 275
Size: 53 83 186 936 1981 382 635 1451
Fragments arranged by size:
1981 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4140 4538 4812 4959 5370 4140
Size: 398 274 147 411 4477
Fragments arranged by size:
4477 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5482
Fragments arranged by size:
5482 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2083 1186
Size: 897 4810
BseYI C'CCAG_C
Cuts at: 2222 4910 5406 2222
Size: 2688 496 2523
Fragments arranged by size:
2688 2523 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5134
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2355 3157 3918 4946 1540
Size: 275 186 354 802 761 1028 2301
Fragments arranged by size:
2301 1028 802 761 354 275 186
BsiHKAI G_wGCw'C
Cuts at: 2130 2236 3768 3853 4924 2130
Size: 106 1532 85 1071 2913
Fragments arranged by size:
2913 1532 1071 106 85
BsmI GAATG_Cn'
Cuts at: 3218 3311 3218
Size: 93 5614
BsmBI CGTCTCn'nnnn_
Cuts at: 2547 2547
Size: 5707
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2130 2236 3026 3768 3853 4924 1391
Size: 399 340 106 790 742 85 1071 2174
Fragments arranged by size:
2174 1071 790 742 399 340 106 85
BspEI T'CCGG_A
Cuts at: 5370 5370
Size: 5707
BspHI T'CATG_A
Cuts at: 3596 5326 3596
Size: 1730 3977
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2283 2328 2573 3062 844
Size: 1439 45 245 489 3489
Fragments arranged by size:
3489 1439 489 245 45
BsrBI CCG'CTC
Cuts at: 1394 1823 2584 3594 1394
Size: 429 761 1010 3507
Fragments arranged by size:
3507 1010 761 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4202 4376 65
Size: 1183 2954 174 1396
Fragments arranged by size:
2954 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2282 4351 4538 1700
Size: 110 472 2069 187 2869
Fragments arranged by size:
2869 2069 472 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5707
BssHII G'CGCG_C
Cuts at: 1925 2987 1925
Size: 1062 4645
BssSI C'ACGA_G
Cuts at: 3767 4779 3767
Size: 1012 4695
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5707
BstBI TT'CG_AA
Cuts at: 4511 4511
Size: 5707
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5707
BstXI CCAn_nnnn'nTGG
Cuts at: 2344 2515 3004 2344
Size: 171 489 5047
Fragments arranged by size:
5047 489 171
BstYI r'GATC_y
Cuts at: 1972 2661 2925 3037 3404 3789 3806 5258 5344
Size: 689 264 112 367 385 17 1452 86
Cuts at: 5344 5356 5702 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 689 385 367 346 264 112
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5155
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4968
Fragments arranged by size:
4968 683 56
BtsI GCAGTG_nn'
Cuts at: 3223 4017 4037 3223
Size: 794 20 4893
Fragments arranged by size:
4893 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5707
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5672
DraI TTT'AAA
Cuts at: 3011 3011
Size: 5707
DraIII CAC_nnn'GTG
Cuts at: 2734 2734
Size: 5707
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2021 4714 809
Size: 507 705 2693 1802
Fragments arranged by size:
2693 1802 705 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2280 2601 2718
Size: 54 1425 50 275 18 450 321 117
Cuts at: 2718 3154 4043 5672 8
Size: 436 889 1629 43
Fragments arranged by size:
1629 1425 889 450 436 321 275 117
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3154 1537
Size: 275 1342 4090
Fragments arranged by size:
4090 1342 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2630 3637 1353
Size: 1277 1007 3423
Fragments arranged by size:
3423 1277 1007
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4294 4668 4814 387
Size: 1064 138 2705 374 146 1280
Fragments arranged by size:
2705 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2128 2128
Size: 5707
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 2309 2630 3770 4367 5154 5422
Size: 393 407 270 321 1140 597 787 268
Cuts at: 5422 1239
Size: 1524
Fragments arranged by size:
1524 1140 787 597 407 393 321 270
268
EcoO109I rG'GnC_Cy
Cuts at: 2502 2502
Size: 5707
EcoRI G'AATT_C
Cuts at: 3016 3394 3016
Size: 378 5329
FspI TGC'GCA
Cuts at: 4213 4213
Size: 5707
HaeII r_GCGC'y
Cuts at: 1678 2939 4854 1678
Size: 1261 1915 2531
Fragments arranged by size:
2531 1915 1261
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4348 4380 4422 4454 1373
Size: 32 135 32 2776 32 42 32 2626
Fragments arranged by size:
2776 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2137 2413 3051 3297 669
Size: 988 281 199 276 638 246 3079
Fragments arranged by size:
3079 988 638 281 276 246 199
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5707
HpaI GTT'AAC
Cuts at: 3297 3297
Size: 5707
KpnI G_GTAC'C
Cuts at: 3032 3032
Size: 5707
MfeI C'AATT_G
Cuts at: 3306 3306
Size: 5707
MluI A'CGCG_T
Cuts at: 5583 5583
Size: 5707
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4821 5005 1305
Size: 3516 184 2007
Fragments arranged by size:
3516 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2720 10
Size: 54 1425 343 888 2997
Fragments arranged by size:
2997 1425 888 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3665 4024 4183 518
Size: 648 2499 359 159 2042
Fragments arranged by size:
2499 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2063 3123 3800 4948 5193 1606
Size: 457 1060 677 1148 245 2120
Fragments arranged by size:
2120 1148 1060 677 457 245
NaeI GCC'GGC
Cuts at: 2284 2284
Size: 5707
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5155
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5707
NgoMIV G'CCGG_C
Cuts at: 2282 2282
Size: 5707
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5707
NotI GC'GGCC_GC
Cuts at: 3154 3154
Size: 5707
NspI r_CATG'y
Cuts at: 1132 1229 3065 4610 1132
Size: 97 1836 1545 2229
Fragments arranged by size:
2229 1836 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2142 1348
Size: 794 4913
PciI A'CATG_T
Cuts at: 4606 4606
Size: 5707
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2716 1108
Size: 1608 4099
PmeI GTTT'AAAC
Cuts at: 3011 3011
Size: 5707
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3776 3808 5289 5321 3776
Size: 32 1481 32 4162
Fragments arranged by size:
4162 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2502 2502
Size: 5707
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5707
PsiI TTA'TAA
Cuts at: 3277 3277
Size: 5707
PstI C_TGCA'G
Cuts at: 830 2393 2570 2706 3059 830
Size: 1563 177 136 353 3478
Fragments arranged by size:
3478 1563 353 177 136
PvuI CG_AT'CG
Cuts at: 2001 2355 2001
Size: 354 5353
SacI G_AGCT'C
Cuts at: 2130 2130
Size: 5707
SalI G'TCGA_C
Cuts at: 1936 3049 1936
Size: 1113 4594
SbfI CC_TGCA'GG
Cuts at: 3059 3059
Size: 5707
ScaI AGT'ACT
Cuts at: 3955 5667 3955
Size: 1712 3995
SfcI C'TryA_G
Cuts at: 826 1045 2389 2566 2702 3055 4190 4871 5062
Size: 219 1344 177 136 353 1135 681 191
Cuts at: 5062 826
Size: 1471
Fragments arranged by size:
1471 1344 1135 681 353 219 191 177
136
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5707
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5707
SmaI CCC'GGG
Cuts at: 1872 2099 3034 1872
Size: 227 935 4545
Fragments arranged by size:
4545 935 227
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3811 4712 4974 820
Size: 197 926 1868 901 262 1553
Fragments arranged by size:
1868 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3498 493
Size: 3005 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5707
SphI G_CATG'C
Cuts at: 1132 3065 1132
Size: 1933 3774
SspI AAT'ATT
Cuts at: 5 52 3631 5
Size: 47 3579 2081
Fragments arranged by size:
3579 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5707
StyI C'CwwG_G
Cuts at: 513 1065 3146 513
Size: 552 2081 3074
Fragments arranged by size:
3074 2081 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2251 3734 3751 3904 4089 4534 325
Size: 1255 671 1483 17 153 185 445 1498
Fragments arranged by size:
1498 1483 1255 671 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2430 3953 5665
Size: 274 80 33 51 1443 453 1523 1712
Cuts at: 5665 96
Size: 138
Fragments arranged by size:
1712 1523 1443 453 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2226 2258 5667 5699 2226
Size: 32 3409 32 2234
Fragments arranged by size:
3409 2234 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2150 1316
Size: 834 4873
XbaI T'CTAG_A
Cuts at: 3043 3043
Size: 5707
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2569 2569
Size: 5707
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5707
XmaI C'CCGG_G
Cuts at: 1870 2097 3032 1870
Size: 227 935 4545
Fragments arranged by size:
4545 935 227
XmnI GAAnn'nnTTC
Cuts at: 3836 3836
Size: 5707
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3515 276
Size: 53 83 186 936 1981 2468
Fragments arranged by size:
2468 1981 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI