Restriction map for
pFN21AE9401
(Circular) (Six-base) MAPSORT of: pFN21AE9401.seq Check: 5398 from: 1 to: 6229
pFN21AE9401 6229 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2024 2185 2024
Size: 161 6068
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2012 3105 4039 278
Size: 53 83 186 936 476 1093 934 2468
Fragments arranged by size:
2468 1093 936 934 476 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3572 1937
Size: 1635 4594
Acc65I G'GTAC_C
Cuts at: 3295 3550 3295
Size: 255 5974
AclI AA'CG_TT
Cuts at: 1563 4356 4729 1563
Size: 2793 373 3063
Fragments arranged by size:
3063 2793 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2876 3431 3507 4292 5676 5944 1632
Size: 1244 555 76 785 1384 268 1917
Fragments arranged by size:
1917 1384 1244 785 555 268 76
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6032
AflIII A'CryG_T
Cuts at: 1203 2052 5128 6105 1203
Size: 849 3076 977 1327
Fragments arranged by size:
3076 1327 977 849
AgeI A'CCGG_T
Cuts at: 5060 5060
Size: 6229
AhdI GACnn_n'nnGTC
Cuts at: 4958 4958
Size: 6229
AleI CACnn'nnGTG
Cuts at: 1166 2279 1166
Size: 1113 5116
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2171 1184
Size: 987 5242
AlwNI CAG_nnn'CTG
Cuts at: 2191 3972 5544 2191
Size: 1781 1572 2876
Fragments arranged by size:
2876 1781 1572
ApaI G_GGCC'C
Cuts at: 3246 3246
Size: 6229
ApaLI G'TGCA_C
Cuts at: 4286 5442 4286
Size: 1156 5073
ApoI r'AATT_y
Cuts at: 1493 3538 3764 3916 6025 1493
Size: 2045 226 152 2109 1697
Fragments arranged by size:
2109 2045 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4783 160
Size: 872 3751 1606
Fragments arranged by size:
3751 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6229
AvaI C'yCGr_G
Cuts at: 1870 1943 3554 1870
Size: 73 1611 4545
Fragments arranged by size:
4545 1611 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3279 3287 3312 3320 3279
Size: 8 25 8 6188
Fragments arranged by size:
6188 25 8 8
BamHI G'GATC_C
Cuts at: 3559 3926 3559
Size: 367 5862
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2465 3295 3550 5005 618
Size: 325 217 627 678 830 255 1455 1842
Fragments arranged by size:
1842 1455 830 678 627 325 255 217
BanII G_rGCy'C
Cuts at: 1391 3246 3548 1391
Size: 1855 302 4072
Fragments arranged by size:
4072 1855 302
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3081 928
Size: 971 1182 4076
Fragments arranged by size:
4076 1182 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4420 4454 1903
Size: 34 2483 34 3678
Fragments arranged by size:
3678 2483 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4121 5331 4121
Size: 1210 5019
BclI T'GATC_A
Cuts at: 1548 3309 1548
Size: 1761 4468
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4840 136
Size: 107 122 71 631 605 3168 1525
Fragments arranged by size:
3168 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6224 6224
Size: 6229
BlpI GC'TnA_GC
Cuts at: 3646 3646
Size: 6229
Bme1580I G_kGCm'C
Cuts at: 1790 3246 4290 5446 1790
Size: 1456 1044 1156 2573
Fragments arranged by size:
2573 1456 1156 1044
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6229
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3132 3295 3960
Size: 416 53 83 399 24 1715 163 665
Cuts at: 3960 4918 442
Size: 958 1753
Fragments arranged by size:
1753 1715 958 665 416 399 163 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6229
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2514 2514
Size: 6229
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4889 1239
Size: 3650 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4354 5219 5517 4354
Size: 865 298 5066
Fragments arranged by size:
5066 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4892 882
Size: 617 3393 2219
Fragments arranged by size:
3393 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2055 4020 493
Size: 711 851 1965 2702
Fragments arranged by size:
2702 1965 851 711
BsaBI GATnn'nnATC
Cuts at: 3209 6223 3209
Size: 3014 3215
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 3102 4036 4418
Size: 53 83 186 936 476 1093 934 382
Cuts at: 4418 5053 275
Size: 635 1451
Fragments arranged by size:
1451 1093 936 934 635 476 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4662 5060 5334 5481 5892 4662
Size: 398 274 147 411 4999
Fragments arranged by size:
4999 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2399 2429 1178
Size: 30 165 30 996 30 4978
Fragments arranged by size:
4978 996 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2213 2447 2487 2780 2930 3002 3156 1186
Size: 1027 234 40 293 150 72 154 4259
Fragments arranged by size:
4259 1027 293 234 154 150 72 40
BseYI C'CCAG_C
Cuts at: 2436 2511 2573 5432 5928 2436
Size: 75 62 2859 496 2737
Fragments arranged by size:
2859 2737 496 75 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2002 2299 2676 3055 1163
Size: 573 266 297 377 379 4337
Fragments arranged by size:
4337 573 379 377 297 266
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3679 4440 5468 1540
Size: 275 186 1678 761 1028 2301
Fragments arranged by size:
2301 1678 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2495 4290 4375 5446 2495
Size: 1795 85 1071 3278
Fragments arranged by size:
3278 1795 1071 85
BsmI GAATG_Cn'
Cuts at: 3740 3833 3740
Size: 93 6136
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2495 3246 3548 4290 4375 5446 1391
Size: 399 705 751 302 742 85 1071 2174
Fragments arranged by size:
2174 1071 751 742 705 399 302 85
BspEI T'CCGG_A
Cuts at: 5892 5892
Size: 6229
BspHI T'CATG_A
Cuts at: 2625 4118 5848 2625
Size: 1493 1730 3006
Fragments arranged by size:
3006 1730 1493
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2024 2185 2244 2460 3471 3584 844
Size: 1180 161 59 216 1011 113 3489
Fragments arranged by size:
3489 1180 1011 216 161 113 59
BsrBI CCG'CTC
Cuts at: 1394 1823 2106 4116 1394
Size: 429 283 2010 3507
Fragments arranged by size:
3507 2010 429 283
BsrDI GCAATG_nn'
Cuts at: 65 1248 2545 4724 4898 65
Size: 1183 1297 2179 174 1396
Fragments arranged by size:
2179 1396 1297 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4873 5060 1700
Size: 110 3063 187 2869
Fragments arranged by size:
3063 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2682 96
Size: 2586 3643
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6229
BssSI C'ACGA_G
Cuts at: 2252 4289 5301 2252
Size: 2037 1012 3180
Fragments arranged by size:
3180 2037 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2191 2677 794
Size: 1397 486 4346
Fragments arranged by size:
4346 1397 486
BstBI TT'CG_AA
Cuts at: 5033 5033
Size: 6229
BstEII G'GTnAC_C
Cuts at: 1184 2197 2447 1184
Size: 1013 250 4966
Fragments arranged by size:
4966 1013 250
BstXI CCAn_nnnn'nTGG
Cuts at: 2086 2374 2086
Size: 288 5941
BstYI r'GATC_y
Cuts at: 1972 3559 3926 4311 4328 5780 5866 5878 6224
Size: 1587 367 385 17 1452 86 12 346
Cuts at: 6224 1972
Size: 1977
Fragments arranged by size:
1977 1587 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2092 2443 2643 513
Size: 552 1027 351 200 4099
Fragments arranged by size:
4099 1027 552 351 200
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5490
Fragments arranged by size:
5490 683 56
BtsI GCAGTG_nn'
Cuts at: 3117 3347 3745 4539 4559 3117
Size: 230 398 794 20 4787
Fragments arranged by size:
4787 794 398 230 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6229
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6194
DraI TTT'AAA
Cuts at: 3533 3533
Size: 6229
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5236 809
Size: 507 3920 1802
Fragments arranged by size:
3920 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2271 2478 2940
Size: 54 1425 50 275 18 441 207 462
Cuts at: 2940 3267 3676 4565 6194 8
Size: 327 409 889 1629 43
Fragments arranged by size:
1629 1425 889 462 441 409 327 275
207 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3676 1537
Size: 275 1864 4090
Fragments arranged by size:
4090 1864 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2294 2739 4159 1353
Size: 941 445 1420 3423
Fragments arranged by size:
3423 1420 941 445
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4816 5190 5336 387
Size: 1064 138 3227 374 146 1280
Fragments arranged by size:
3227 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2876 3431 3507 4292 4889 5676 5944
Size: 393 1244 555 76 785 597 787 268
Cuts at: 5944 1239
Size: 1524
Fragments arranged by size:
1524 1244 787 785 597 555 393 268
76
EcoO109I rG'GnC_Cy
Cuts at: 3242 3243 3242
Size: 1 6228
EcoRI G'AATT_C
Cuts at: 3538 3916 3538
Size: 378 5851
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3436 3436
Size: 6229
FspI TGC'GCA
Cuts at: 4735 4735
Size: 6229
HaeII r_GCGC'y
Cuts at: 1678 2165 2443 5376 1678
Size: 487 278 2933 2531
Fragments arranged by size:
2933 2531 487 278
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4870 4902 4944 4976 1373
Size: 32 135 32 3298 32 42 32 2626
Fragments arranged by size:
3298 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2362 3028 3573 3819 669
Size: 988 281 424 666 545 246 3079
Fragments arranged by size:
3079 988 666 545 424 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6229
HpaI GTT'AAC
Cuts at: 3819 3819
Size: 6229
KpnI G_GTAC'C
Cuts at: 3299 3554 3299
Size: 255 5974
MfeI C'AATT_G
Cuts at: 3828 3828
Size: 6229
MluI A'CGCG_T
Cuts at: 6105 6105
Size: 6229
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5343 5527 1305
Size: 4038 184 2007
Fragments arranged by size:
4038 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2273 2480 2942 10
Size: 54 1425 343 441 207 462 3297
Fragments arranged by size:
3297 1425 462 441 343 207 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2057 2279 2372 2805 3357 4187 4546
Size: 648 891 222 93 433 552 830 359
Cuts at: 4546 4705 518
Size: 159 2042
Fragments arranged by size:
2042 891 830 648 552 433 359 222
159 93
MspA1I CmG'CkG
Cuts at: 1606 2188 2445 3645 4322 5470 5715 1606
Size: 582 257 1200 677 1148 245 2120
Fragments arranged by size:
2120 1200 1148 677 582 257 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5677
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6229
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6229
NotI GC'GGCC_GC
Cuts at: 3676 3676
Size: 6229
NspI r_CATG'y
Cuts at: 1132 1229 2062 2675 3376 3409 3587 5132 1132
Size: 97 833 613 701 33 178 1545 2229
Fragments arranged by size:
2229 1545 833 701 613 178 97 33
PasI CC'CwG_GG
Cuts at: 1348 2262 3238 1348
Size: 914 976 4339
Fragments arranged by size:
4339 976 914
PciI A'CATG_T
Cuts at: 5128 5128
Size: 6229
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2559 1108
Size: 1451 4778
PfoI T'CCnGG_A
Cuts at: 2772 3120 3399 2772
Size: 348 279 5602
Fragments arranged by size:
5602 348 279
PmeI GTTT'AAAC
Cuts at: 3533 3533
Size: 6229
PmlI CAC'GTG
Cuts at: 2055 2055
Size: 6229
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4298 4330 5811 5843 4298
Size: 32 1481 32 4684
Fragments arranged by size:
4684 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3012 1613
Size: 1399 4830
PsiI TTA'TAA
Cuts at: 3799 3799
Size: 6229
PspOMI G'GGCC_C
Cuts at: 3242 3242
Size: 6229
PstI C_TGCA'G
Cuts at: 830 2196 2244 2490 3342 3581 830
Size: 1366 48 246 852 239 3478
Fragments arranged by size:
3478 1366 852 246 239 48
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6229
PvuII CAG'CTG
Cuts at: 2188 2188
Size: 6229
SacII CC_GC'GG
Cuts at: 2446 2446
Size: 6229
SalI G'TCGA_C
Cuts at: 1936 3571 1936
Size: 1635 4594
SbfI CC_TGCA'GG
Cuts at: 3581 3581
Size: 6229
ScaI AGT'ACT
Cuts at: 4477 6189 4477
Size: 1712 4517
SfcI C'TryA_G
Cuts at: 826 1045 2192 2240 2486 3338 3577 4712 5393
Size: 219 1147 48 246 852 239 1135 681
Cuts at: 5393 5584 826
Size: 191 1471
Fragments arranged by size:
1471 1147 1135 852 681 246 239 219
191 48
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6229
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6229
SmaI CCC'GGG
Cuts at: 1872 3556 1872
Size: 1684 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4333 5234 5496 820
Size: 197 926 2390 901 262 1553
Fragments arranged by size:
2390 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4020 493
Size: 3527 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6229
SphI G_CATG'C
Cuts at: 1132 2062 2675 3587 1132
Size: 930 613 912 3774
Fragments arranged by size:
3774 930 912 613
SspI AAT'ATT
Cuts at: 5 52 4153 5
Size: 47 4101 2081
Fragments arranged by size:
4101 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6229
StyI C'CwwG_G
Cuts at: 513 1065 2820 2841 2862 3668 513
Size: 552 1755 21 21 806 3074
Fragments arranged by size:
3074 1755 806 552 21 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2254 2351 3038 3151 3265 3295 3471
Size: 1255 674 97 687 113 114 30 176
Cuts at: 3471 4256 4273 4426 4611 5056 325
Size: 785 17 153 185 445 1498
Fragments arranged by size:
1498 1255 785 687 674 445 185 176
153 114 113 97 30 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2682 4475 6187
Size: 274 80 33 51 1443 705 1793 1712
Cuts at: 6187 96
Size: 138
Fragments arranged by size:
1793 1712 1443 705 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2399 2431 6189 6221 2399
Size: 32 3758 32 2407
Fragments arranged by size:
3758 2407 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6229
XbaI T'CTAG_A
Cuts at: 3565 3565
Size: 6229
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2508 2508
Size: 6229
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6229
XmaI C'CCGG_G
Cuts at: 1870 3554 1870
Size: 1684 4545
XmnI GAAnn'nnTTC
Cuts at: 3416 3419 4358 3416
Size: 3 939 5287
Fragments arranged by size:
5287 939 3
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2010 3103 4037 276
Size: 53 83 186 936 476 1093 934 2468
Fragments arranged by size:
2468 1093 936 934 476 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PpuMI PspXI PsrI RsrII SacI SanDI SapI
SexAI SfiI SfoI SrfI SwaI